AN 5107 Histology Lab Blood and Hematopoiesis

Be able to identify peripheral blood cells (erythrocytes, lymphocytes, monocytes, eosinophils, neutrophils, basophils) and platelets and describe their functions (Pg 94-95) Erythrocytes RBCs (red blood corpuscles) o Transport O2 and CO2 in blood o No nucleus and live about 120 days o 7.5 um lots of them Leukocytes (WBC) use blood for transportation but function outside of it o 2 types Granulocytes and Agranuloctyes o Granulocytes Neutrophils 2-5 lobed nucleus will see granules but will be lighter 12-15um bigger than RBC Phagocytes of bacteria and small particles Basophils doesnt really have a distinct lobed nucleus Heavily granulated with histamine and heparin o Inflammatory response Eosinophil bilobed nucleus Medium dark stain on granules Associated with allergic, inflammatory, and parasitic infection o Agranulocytes Lymphocytes dark nucleus, thin rim 6-18um Looks like a big eye, tadpole egg Courses through blood to tissue and back Monocytes In blood 12-20um big Oval or kidney bean shaped nucleus when in connective tissue = Macrophage Platelets 2-4um small o No nucleus o Fragments of megakaryocytes o Clotting and vessel repair Be able to identify peripheral blood from bone marrow Blood when in blood we will see way more RBC and will only see 1-3 Leukocytes on a slide Bone Marrow when in bone marrow you will see few RBC and will also many more Leukocytes in a small area, as well as a variety of Leukocytes Be able to identify the 5 cells (immature) of the erythrocyte cell line (Pg 97 and 100) Proerythroblast Located in bone marrow (earliest stage) o Large cell with large nucleus Nucleus virtually takes up whole cell, no cytoplasm visible Basophilic Erythroblast Located in bone marrow o Like proerythroblast but is a smaller cell, still has big nucleus

looks granulated and no cytoplasm Polychromatophilic Erythroblast located in bone marrow (intermediate stage in development) o Cell has shrunk and now is a little bigger than a RBC o Nucleus has shrunk in size still stain dark, can now see cytoplasm light stain Orthochromatophilic Erythroblast Located in bone marrow o Smaller than previous cells o Dark condensed nucleus may be in the process of being expelled On the edge of the cell Reticulocyte Located in bone marrow o Pretty much resembles a circulating RBC o No nucleus

Be able to identify the myeloblast, promyelocyte, and band (stab) cells of the granulocyte cell line (Pg 97 and 101) Myeloblast Granulocyte Located in bone marrow (1st stage) o Has a large nucleus, but can see cytoplasm which stains darker than erythroblast Promyelocyte Granulocyte Located in bone marrow o Cytoplasm lighter color but contains numerous granules o Nucleus has shrunk in size, possible a little bean shape Stab cell located in bone marrow (granulocyte) o Neutrophilic stab cell Nucleus will be dark and horseshoe-shaped, light color cytoplasm o Eosinophilic - heavily granulated , cant see cytoplasm Be able to identify a megakaryocyte and describe its function Megakaryocyte derived from Megakaroblast o Very large in size 15-50um o Large oval/kidney shaped nucleus o Platelets are fragments that are shed from this. Be responsible for all functions/characteristics mentioned in lecture that are relevant to the structures mentioned above.

Lymphoid Tissue
Be able to recognize and describe the histologic features of the thymus gland Thymus: Capsule Indicator of a Primary lymphatic organ or tissue Cortex - Darker colored area surround the medulla o This is where immature T-cells are stored, most will die only a select few will make it to the medulla and become immunocompetent Medulla consist of epithelial reticular cells, mature T-cells and Hassalls corpuscles o Epithelial reticular cells form a thymic-blood barrier (prevents antigens from entering the thymic cortex No nodules Be able to recognize and describe the histologic features of diffuse lymphatic tissue and the lymphatic nodule These lymphatic tissues are found in systems to exterior (MALT) mucosa-associated lymphatic tissue Diffuse lymphatic tissue o Lymphatic tissue not enclosed by a capsule Be able to recognize and describe the histologic features of the lymph node and spleen Be able to discuss the types of cells found in lymphatic tissue Be able to recognize lymphatic vessels Be responsible for all functions/characteristics mentioned in lecture that are relevant to the structures mentioned above.

References: Textbook: Junqueira and Carneiro Atlas: Gartner and Hiatt Histo-time Websites: http://www.bu.edu/histology/m/index.htm http://medic.med.uth.tmc.edu/edprog/histology/carousel.htm http://www.siumed.edu/~dking2/indes.htm