DNA

RECOMBINANT
Jauhali, Miranda, Nitro, Perez, Rentura, Turco
GROUP 2 (BSMT 2A)
DNA RECOMBINANT
Recombinant DNA (rDNA) is a
technology that uses enzymes
to cut and paste together DNA
sequences of interest. The
recombined DNA sequences
can be placed into vehicles
called vectors that ferry the
DNA into a suitable host cell
where it can be copied or
expressed.
GOALS OF RECOMBINANT
DNA TECHNOLOGY
To isolate and characterize a gene.
To make a desired alterations in one or more isolation
genes.
To return altered genes to living cells.
Artificially synthesize new gene.
Alternating the genome of an organism.
Understanding the hereditary dieases and their cure.
Improving human genome.
procedure of making RDNA
1) 3)
iSOLATING JOINING
OF DNA OF DNA
2) 4)
CUTTING AMPLIFYING
OF DNA OF DNA
ISOLATING OF DNA
CUTTING OF DNA
DNA can be cut into large fragments
by mechanical shearing.
Restriction of enzymes are the scissors
of molecular genetics.
RESTRICTION ENZYME
A special class of sequence-
specific enzyme.
Found in bacteria
Site-specific-cleave DNA
molecules only at specific
nucleotide sequence.
REases recognize DNA base
sequence that are palindrome.
REase make staggered cuts
with complementary base
sequences for easy circulization.
JOINING DNA
AMPLIFYING THE
RECOMBINANT DNA
Transforming the recombinant DNA into
a bacterial host strain.
The cells are treated with CaCl2.
DNA is added.
Cells are heat shocked at 42C
DNA goes into cell by somewhat unknown
mechanism.
Once in a cell, the recombinant DNA will
be replicated.
When the cell divides, the replicated
recombinant molecules go to both
daughter cells which themselves will divide
later. Thus, DNA is amplified.
 Activity:
With the words provided above, complete the diagram.

NOTE: Only the

leaders will input
the answers in the
chat box.
DOCUMENTATION