Practical 4

Study of gamete development in mouse

Aim - To study gamete development in T.S. of Testis and V.S. of ovary using permanent slides.

Requirements - Permanent slides of T.S. of Testis and V.S. of ovary, compound microscope.

Observations -
A. T.S. of Testis -

1. The testes comprise several seminiferous tubules embedded in the interstitial tissues.
2. Thick fibrous tissues called tunica albuginea cover the testes.
3. It comprises different types of cells from the outside to the lunar in the manner given below:
Spermatogonia → Spermatocytes → Spermatids → Spermatozoa (sperms)

4. Sertoli cells are located between the germinal cells.

5. The Leydig cells that produce testosterone are present in the interstitial tissues.

B. V.S. of ovary -

1. An ovary is a germinal epithelium bounded by a solid structure covered by a thick layer of

fibrous tissue known as tunica albuginea.
2. It consists of an inner medulla and an outer cortex.
3. The medulla comprises several round or oval bodies known as ovarian follicles.
4. Follicle development takes place in the following stages:

Primary follicle → Secondary follicle → Tertiary follicle → Graffian follicle → Corpus luteum

5. Cortex comprises corpus luteum along with mature follicles.

 Practical 5
Study of T.S. of blastula

Aim - To study the T.S. of blastula using permanent slides.

Requirements - Permanent slide o T.S. of blastula, compound microscope.

Observations -
1. In transverse section, the blastula appears as a sphere with a cavity, called blastocoel within it.
2. An outer layer of blastomeres is called trophoblast.
3. A cellular mass, adhered to the trophoblast is present on one end of the blastula. It is called inner
cell mass.