B.

Coenzyme
ENZYMES C. Activator
For video explanation visit D. Cofactor

our youtube channel DR 7. Enzymes _________ the activation energy of

a chemical reaction
MOHSIN JAVED A. Increases
B. Decreases
1. Biological molecules (proteins) which C. Does not effect
catalyze a biochemical reaction and remain D. Increases or decreases depending upon
unchanged after completion of reaction are individual enzyme
called
A. Cofactor 8. A three-dimensional cavity bearing a specific
B. Coenzymes charge by which the enzyme reacts with its
C. Activator substrate is called
D. Enzymes A. Active site
B. Binding site
2. Which statement about enzyme is incorrect: C. Catalytic site
A. Some of them consist solely of protein with D. Allosteric site
no non-protein part.
B. They catalyze a chemical reaction without 9. Which step causes the activation of the
being utilized. catalytic site of an enzyme?
C. All enzymes are fibrous Proteins. A. Change in pH of the surroundings.
D. They without their cofactor are called B. Formation of Enzyme Substrate complex.
apoenzyme. C. Change in the charge of the active site.
D. Change in temperature
3. In which of the following location enzymes
controlling cellular respiration are present? 10. Lock and Key model was proposed by
A. Nucleus A. Emil Fischer
B. Chloroplast B. Koshland
C. Mitochondria C. Robin Williams
D. Ribosome D. Rudolph Virchow

4. An activated enzyme consisting of the 11. Which statement is incorrect about Lock and
polypeptide chain and a cofactor is called: Key Model?
A. Apoenzyme A. The specific enzyme can transform only a
B. Holoenzyme specific substrate
C. Activated enzyme B. The active site of an enzyme is a non-flexible
D. Both B and C structure.
C. The active site does not change before during
5. Which one forms the raw material for or even after the reaction.
coenzymes? D. It explains the mechanism of every chemical
A. Vitamins reaction.
B. Carbohydrates
C. Proteins 12. The rate of reaction is directly proportional
D. Metals to the concentration of an enzyme which
statement is incorrect in this respect:
6. A cofactor made of inorganic ion which is A. Increase in enzyme molecule increases the
detachable is called available active sites.
A. Prosthetic group B. This relation is for an unlimited time period
with unlimited enzyme concentration
C. If the concentration is doubled the rate will
become two-fold. 18. The extreme change in pH results in:
D. None of these. A. Change in ionization of amino acids at the
active site of the enzyme.
13. If the concentration of enzyme is kept B. Change in the ionization of the substrate.
constant and amount of substrate is increased a C. Denaturation of the enzyme
point is reached where an increase in substrates D. Increase in the reaction rate.
concentration does not affect the reaction rate
because of 19. Optimal temperature of enzymes present in
A. Enzymes get denatured at higher substrate the human body is
concentration. A. 27°C
B. The rate of reaction is indirectly proportional B. 37°C
to substrate concentration at this point. C. 47°C
C. All the active sites on enzyme molecule are D. 30°C
occupied.
D. None of these. 20. A chemical substance which can react (in
place of substrate) with the enzyme but is not
14. If more substrate to already occurring transformed into product/s and thus blocks the
enzymatic reaction is added more enzyme active site temporarily or permanently is called
activity is seen because: A. Co-enzyme
A. There is probably more substrate present than B. Blocker
there is an enzyme. C. Inhibitor
B. There is probably more enzyme available D. Cofactor
than there is the substrate.
C. There is probably more product present than 21. Inhibitors which block the enzyme by
there is either substrate or enzyme. forming a weak bond are called
D. The enzyme-substrate complex is probably A. Competitive inhibitors.
failing to form during the reaction. B. Non-competitive inhibitors
C. Irreversible inhibitors.
15. If more substrate to already occurring D. Both a and b
enzymatic reaction is added and there is no
effect on the rate of the reaction what is the form 22. A substance which binds at the active site of
given to this situation: the enzyme but does not result in the formation
A. Saturation of the products is called:
B. Denaturation A. Irreversible inhibitor
C. Composition B. Reversible inhibitor
D. Inhibition C. Competitive inhibitor
D. Non-competitive inhibitor
16. The active site of an enzyme:
A. Never changes 23. The structure of an enzyme is altered by:
B. Forms no chemical bond with the substrate A. Irreversible inhibitor
C. Determined by the structure and the B. Reversible inhibitor
specificity of the enzyme. C. Competitive inhibitor
D. They are non-specific in their action. D. Non-competitive inhibitor

17. Excessive increase in temperature of the 24. Malonic acid is an example of:
medium causes the enzyme molecule to A. Irreversible inhibitor
A. Activate B. Reversible inhibitor
B. Unaffected C. Competitive inhibitor
C. Denatured D. Non-competitive inhibitor
D. None of these.
25. If enzyme concentration is low than substrate b. Basic, basic
pH and temperature values are equal to c. Basic, acidic
requirement then which of the following will d. Acidic, basic
increase the rate of reaction.
A. Increase in the concentration of enzyme 32. Prosthetic group is a part of conjugated
B. Increase in the concentration of substrate enzyme it is:
C. Increase in pH a. loosely attached inorganic part
D. Increase in temperature b. loosely attached organic part
c. firmly attached proteins part
26. The enzymes amylase, lipase, and protease d. firmly attached non-protein part
best describe as:
a. ligase 33. Which one of the following is the correct
b. lysase statement with reference to enzyme.
c. Hydrolysase a. apoenzyme = holoenzyme + coenzyme
d. isomerase b. holoenzyme = apoenzyme + coenzyme
e. Oxidoreductase c. coenzyme =apoenzyme + coenzyme
d. holoenzyme = coenzyme+cofactor
27. All of the following are the ligase enzymes
EXCEPT. 34. Which enzyme in the following shows the
a. Ribozymes greatest substrate specificity?
b. DNA-polymese a. Nuclease
c. RNA polymerase b. pepsin
d. All of these c. trypsin
e. None of these d. sucrase

28. An enzyme that convert Glucose-6-posphate 35. An enzyme act by:

into fructose-6-posphate would be a ……. a. reducing the energy of activation
enzyme. b. increasing the energy of activation
a. ligase c. Decreasing the pH
b. lysase d. increasing the pH
c.Hydrolysase
d. isomerase 36. coEnzyme is:
e. Oxidoreductase a. Always a protein
b. often a vitamin
29. Which part of a ribsome is catalytic in the c. often a metal
future? d. always an inorganic compound
a. Nucleic acid part
b. Protein in part 37. Which of the following has the least
c. Lipid part possibility to have enzyme within it:
d. none of these a. Algae
b. Bacteria
30. What is correct for substrates for pepsin and c. Fungi
trypsin respectively? d. Viruses
a. Lipids, proteins
b. protein, proteins 38. All of the following are correct for pepsin
c. Proteins, carbohydrates EXCEPT.
d. lipids, lipids a. found active in the stomach
b. work at about 2pH in an acidic environment
31. What is correct for the pH environment for c. convert the long chain of proteins into the
pepsin and trypsin respectively? short chain of proteins
a. Acid, acid d. It’s a member of hydrolytic enzymes.
e. All are correct statements for the pepsin 46. Activators are:
enzyme. a. prosthetic group
b. organic co-factors
39. Find the incorrect statements for enzymes. c. inorganic co-factors
a. shows maximum work at optimum d. All a, b and c
temperature and pH
b. Freeze at very low-temperature 47. Active site of an enzyme may contain.
c. Denatured at very high temperatures. a. 2-3 amino acids
d. Freeze at a very low pH. b. 3-12 amino acids
e. All statements are correct regarding enzymes. c. 70-90 amino acids
d. 100s to 1000s amino acids
40. Enzymes functional inside the cell are
termed as: 48. An enzyme that joins the ends of two strands
a. Endoenzymes of nucleic acid is:
b. Exoenzymes A. Polymerase
c. Apoenzymes B. ligase
d. Isoenzymes C. synthetase
D. Helicase
41. Hydrolysis of starch occurs with the help of:
a. Peptidase 49. Blocking of enzyme action by blocking its
b. Amylase active site is called as:
c. surcease A. Allosteric inhibition
d. lipase B. Feedback inhibition
C. Competitive inhibition
42. In the cell, the digestive enzyme mostly D. Non-competitive inhibition
occur in:
a. mitochondria 50. Enzyme complex involved in alcoholic
b.lysosemes fermentation is:
c. ribosomes A. Zymase
d. plastids B. Invertase
C. Lipase
43. A conjugated enzyme minus its coenzyme is D. Amylase
known as:
a. Apoenzyme
b. Metalloenzyme
c. Isoenzyme
d. All of these

44. Which of the following is not an attribute of

enzymes:
a. specific in nature
b. protein in chemistry
c. consumed in reaction
d. increase rate of reaction

45. Fat is hydrolyzed by the enzyme known as:

a. trypsin
b. lipase
c. pepsin
d. Amy lase