Microscope

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Microscope:

A microscope is an instrument that can be used to observe specimens such as


microorganisms and cells that cannot be seen with your naked eye. The image of an
object is magnified through at least one lens in the microscope. This lens bends light
toward the eye and makes an object appear larger than it actually is.

Light Source:

The light source helps to light up the specimen so that it can easily be seen through the
microscope although you should not keep it on for long as it might burn. Some
microscopes have a built-in light and some require a mirror and a lamp. Light is
collected by a mirror at the base of the microscope. The mirror is held in special joints
that allow it to move in any direction. The light comes from a lamp or from a sunless
sky. It must never be collected directly from the Sun as this can cause severe eye
damage and blindness.

Slide under Microscope:

A specimen for viewing under the microscope must be put on a glass slide. The slide is
put on the stage and held in place by stage clips. The slide should be positioned so that
the specimen is in the center of the hole in the stage.

Stage clips:

They hold the microscope slide on the stage.

Stage:

The microscope slide is placed here to be observed.

Base:

It provides stability and holds all essential components of the microscope.

Arm:

It supports the microscope. This part should be held when the microscope is carried.

Ocular tube:

It connects the eyepiece lens to the objective lens. The light coming from objectives will
bend inside this tube. In binocular microscopes, they are adjustable so that the viewer
can adjust the eyepiece for maximum visualization. It is located above the stage and
has an eyepiece lens on top and objective lens at the bottom. The magnification of the
two lenses is written on them.
Eyepiece lens:

It is the part where the eye looks into to view the magnified specimen. An eyepiece lens
may give a magnification x5 or x10.

Objective lens:

The objective lens magnifies(enlarges) the specimen. The magnification provided by b


Oth the eyepiece lens and the objective lens is found by multiplying their magnifying
power. Most microscopes have three objective lenses on a nosepiece at the bottom part
the ocular tube. The nosepiece can be rotated to bring each objective lens under the
ocular tube in turn. An investigation with the microscope always starts by using the
lowest power objective lens, and then working up to the highest power objective lens if it
tis requires.

Fine and coarse adjustment knob (focusing knob):

The view of the specimen is brought into focus by turning the focusing knob on the side
of the microscope. Basically, they are turned to get a sharp, clear and magnified view of
the specimen. This may raise or lower the ocular tube, or it may raise or lower the
stage, in either case we should watch the specimen from the side of the microscope as
you turn the knob to bring the objective lens and the specimen close together so that
they do not touch and the slide does not break. When the objective lens and the
specimen are close together but not touching look down the eyepiece and turn the
focusing knob so that the objective lens and the specimen move apart. If you do this
slowly the blurred image will slowly become clear.

Q3) You would get a total magnification of x15

Q4) In light compound microscope in order to ascertain the total magnification when
viewing an image with a compound light microscope, take the power of the objective
lens which is at 4x, 10x or 40x and multiply it by the power of the eyepiece which is
typically 10x. Therefore, a 10x eyepiece used with a 40X objective lens, will produce a
magnification of 400X. The naked eye can now view the specimen at a magnification
400 times greater and so microscopic details are revealed. So, light compound
microscope with 15X eye piece lens and 45X objective lens will have a magnification of
675.

Q5) we can use three objective lenses on the nose piece:

Explanation:

 Objective lenses are the optical element closest to the specimen.


 Objectives glasses is used in microscope telescope etc.

 Usually, we can find 3 or 4 objective lenses on a microscope. these are


consisted of 4x, 10x, 40x and 100x powers by changing these we can
magnify more.

Here the function of nose piece is that it is one of the parts of a microscope it is
responsible for holding the objective lenses. they can be used to change the
magnification as well by changing the objective lens by revolving the nosepiece.

Q6) While you look down the microscope, it racks the lens away from the slide. And
stops when something comes into focus. This is what you are meant to be seeing, but it
could be dust on the bottom of the slide or the top of the cover slip. The dust can also
be on the condenser lenses on the light bulb.

Q7) The light then passes through the object and is refracted by the objective lens. The
objective lens is the more powerful lens of a microscope and is closest to the object.
The light then travels to the ocular lens, which focuses the image onto the user's eye.

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