BONUS BTR

MICROBIOLOGY
MICROSCOPES

Fixative:
Slide: Fixative:
Resolution: Slide:
Magnification: Resolution:
Internal structures:
3D image:
Max magnification:
MICROSCOPES

UV light
Dichroic mirror
Auramine/Rhodamine:
Acridine orange:
Calcoflour white:
FITC:

Auto-fluorescence:
Cyclospora
NADPH
STAINS
Primary:
Mordant:
Decoloriser:
Counterstain:

ACID FAST-
TB:
Leprosy:
Nocardia, Legionella:
Crypto/ Isospora/ Bacterial spores/Head of
sperm:
COLD:

SPORES:
Schaeffer and Fulton stain
Ashby stain

FLAGELLA: Leifson and Ryu

Levaditi
SPORES: Ponder Albert Neisser
Fontana
BACTERIAL WALL

O
C
A

DIAMINOPIMETIC ACID Linulus amebocyte assay (LAL):

BACTERIAL WALL
Capsule:Polysaccharide
SHIN

Klebsiella
C.perfringes
B.pertussis
Y.pestis
V.parahemolyticus

Polypeptide:
Hyaluronic acid:

Cell wall deficient

Protoplast
Spheroplast
Stable:
ENDOTOXIN-EXOTOXIN

Low antigenicity. Toxicity

No toxoid, vaccine
Heat stable
Released by lysis
FLAGELLA

Vibrio
Pseudomonas

H.Pylori, Bartonella bacilliformis

Spirilla

Listeria AGAR:
E.Coli Solid:
Clostridium Prevent swarming:
B.cereus Nutrition?
Proteus
BACTERIAL GROWTH
CULTURE MEDIA
ENRICHED (Solid) SELECTIVE
TRANSPORT
Blood Crystal violet
Cary-Blair, VR
Chocolate LJ
Sach’s buffered glycerol saline
LSS Thayer martin
BHI Cetrimide
Pike
PLET
Stuart, Amies
ENRICHMENT (Liquid) MYPA
Selenite F, Tetrathionate broth Deoxycholate citrate, XLD
Alkaline peptone water Potassium tellurite (McLeod)
Maconkey (S,D,I)
CLED (S, D)
Mannitol salt (S,D, I)
TCBS (S,D)
CULTURE MEDIA
CULTURE MEDIA

Lawn culture
Stab
Streak

Colony morphology:

Fish eye-
Dinger’s ring (EMJH)-
Bissected pearl-
Fried egg-
BACTERIAL GENETICS

Antibiotic resistance
Bacteriocins
Enterotoxin production

Virulent phage:
Temperate phage:
TOXINS CODED BY LYSOGENIC PHAGE
Antigen-Antibody reactions
Soluble Ag: PRECIPITATION/FLOCCULATION:
Particulate Ag: Ring test: Ascoli Lancefield
Slide: VDRL
Tube: Kahn
Immunodiffusion/ Gel: Elek test
Rocket electrophoresis

AGGLUTINATION
Slide: Blood grouping
Tube: Widal Weil Felix Paul-Bunnel CAT SAT MAT

Coombs test
Indirect/Passive agglutination:
Latex-ASO, CRP, RF, HCG
Heme-Rose Waaler test
Mancini
Complement fixation:
Wassermann, TPI
Sabin Feldman
Ouchterlony

Oudin Oakley-Fulthrope
ELISA / IF / RIA
BACTERIOLOGY
BACTERIOLOGY
BACTERIOLOGY

Jet-actin Iridiscent colonies

motility
VIROLOGY
DNA VIRUSES
RNA VIRUSES:
ssRNA
dsDNA ssDNA
SINGLE COPY

Nonenveloped Enveloped UNSEGMENTED

Nonenveloped
Smallest

Replicates
through an RNA Nucleic acid replication
intermediate DNA RNA
Largest
Complex
symmetry
Cytoplasm
VIROLOGY
Shipyard eye:
Devil’s grip:
Torres bodies
Breteau index
PARASITOLOGY
 PARASITOLOGY
SKIN TESTS

Casoni-
Montenegro-
Mazzotti-
Bachmann
Fairly
Mosquito bite: Ingestion-
Fullborne
Cat feces: Frenkel
Transfusion: Meat:
Vertical:
Placental transfer:
 MYCOLOLGY – Morphological Classification
YEAST YEAST - LIKE MOLD DIMORPHIC

B-Glucan Galactomannan