WAVELENGTH ACCURACY REPORT

A C Q U I S I TI O N I N F O R M A TI O N

System Name: e2695_2489 MSG

Project Path: RC2_SQT _e2695_2489 final
Processing Method: 030 Wavelength Accuracy PM
Sample Kit Expiration Date: Friday, August 16, 2019 12:00:00 AM EDT
Sample Kit Lot Number: W16081621
Report Method: 030 Wavelength Acc RM Acquired By: Melissa
Sample Set Name: Wavelength Accuracy SSM Column Serial Number: 01833630013817
Sample Set Id: 6124 Result Set Id: 6135

Wavelength accuracy is determined by identifying the two (2) lambda max values of Caffeine. The spectrum of
Caffeine is well defined with a lambda max of 205nm and 273nm. The deviation from these wavelengths is
measured to determine the accuracy of the detector. In combination with power-on diagnostic, the SystemsQT
provides accuracy verification across a range of 205nm to 656nm.

The test passes if the measured wavelengths are within ±2 nm of each Caffeine lambda max values.

Results are rounded to the same precision as displayed by the acceptance criterion prior to comparing against
the specification.

Wav elength Accuracy Results

W avelength Accuracy Lambda Max Lambda Max
Spec 1 (nm) Pass/Fail
1 203nm-207nm 205 PASS

W avelength Accuracy Lambda Max Lambda Max

Spec 2 (nm) Pass/Fail
1 271nm-275nm 274 PASS

Waters Project Name and Version: SQT_E_2695_2489 v4.00 © 2007 - 2017 Waters Corporation. All rights reserved.
Date: Monday, February 20, 2017
Printed: 11:16:50 AM US/Eastern Page: 1 of 3
 WAVELENGTH ACCURACY REPORT

A C Q U I S I TI O N I N F O R M A TI O N

System Name: e2695_2489 MSG Sample Set Id: 6124

Project Path: RC2_SQT_e2695_2489 final Result Set Id: 6135

Wav elength Accuracy Ov erlaid Chromatograms (Caffeine peak) 202 nm - 208 nm

(Ov erlaid Chromatograms hav e offset X axis)

1.50

1.00
AU

0.50

0.00

Wav elength Accuracy Ov erlaid Chromatograms (Caffeine peak) 270 nm - 276 nm

(Ov erlaid Chromatograms hav e offset X axis)
0.70

0.60

0.50

0.40
AU

0.30

0.20

0.10

0.00

Waters Project Name and Version: SQT_E_2695_2489 v4.00 © 2007 - 2017 Waters Corporation. All rights reserved.
Date: Monday, February 20, 2017
Printed: 11:16:50 AM US/Eastern Page: 2 of 3
 WAVELENGTH ACCURACY REPORT

A C Q U I S I TI O N I N F O R M A TI O N

System Name: e2695_2489 MSG Sample Set Id: 6124

Project Path: RC2_SQT_e2695_2489 final Result Set Id: 6135

Wav elength Accuracy Results

Injection
Inj ection W avelength Height Area
Sample Name Vial Volume Channel Name Inj ection Id Result Id
Number (nm) (µV) (µV*sec)
(µL)
1 Caffeine 0.06 mg/mL 74 1 10.00 W2489 ChA 205 1150766.82 4707041.09 6145 6198
2 Caffeine 0.06 mg/mL 74 1 10.00 W2489 ChA 204 1146756.55 4696259.65 6141 6197
3 Caffeine 0.06 mg/mL 74 1 10.00 W2489 ChA 206 1138427.12 4657535.52 6149 6199
4 Caffeine 0.06 mg/mL 74 1 10.00 W2489 ChA 203 1129937.74 4619094.63 6137 6193
5 Caffeine 0.06 mg/mL 74 1 10.00 W2489 ChA 207 1119450.23 4574984.02 6153 6200
6 Caffeine 0.06 mg/mL 74 1 10.00 W2489 ChA 202 1096354.49 4486079.97 6132 6192
7 Caffeine 0.06 mg/mL 74 1 10.00 W2489 ChA 208 1083110.24 4427018.19 6157 6201

Wav elength Accuracy Results

Injection
Inj ection W avelength Height Area
Sample Name Vial Volume Channel Name Injection Id Result Id
Number (nm) (µV) (µV*sec)
(µL)
1 Caffeine 0.06 mg/mL 74 1 10.00 W2489 ChA 274 429574.45 1754184.85 6177 6206
2 Caffeine 0.06 mg/mL 74 1 10.00 W2489 ChA 273 428902.22 1758990.37 6173 6205
3 Caffeine 0.06 mg/mL 74 1 10.00 W2489 ChA 272 428019.90 1755856.09 6169 6204
4 Caffeine 0.06 mg/mL 74 1 10.00 W2489 ChA 271 427313.74 1753386.84 6165 6203
5 Caffeine 0.06 mg/mL 74 1 10.00 W2489 ChA 275 423567.86 1737309.30 6181 6207
6 Caffeine 0.06 mg/mL 74 1 10.00 W2489 ChA 270 419414.76 1723722.68 6161 6202
7 Caffeine 0.06 mg/mL 74 1 10.00 W2489 ChA 276 416927.70 1709478.66 6185 6208

Waters Project Name and Version: SQT_E_2695_2489 v4.00 © 2007 - 2017 Waters Corporation. All rights reserved.
Date: Monday, February 20, 2017
Printed: 11:16:50 AM US/Eastern Page: 3 of 3