Repetability-(Beer)

Concentration Area
0.50 33.61
1.00 64.42
2.50 147.29
5.00 280.69
10.0 578.04

HYPERICIN
700

600
f(x) = 57.0134328358209 x + 4.15895522388061
500 R² = 0.999496705632019
400
Area

300

200

100

0
0.00 2.00 4.00 6.00 8.00 10.00 12.00
Concentration

Analysed By Verified By
 Repetability - Hypericin(BEER)
Sample Weight Make up volume Volume taken Concentration Result Spike Con. Spike and Recoevry REPEATABILITY LEVES
1.09105 20 5 1.310 4.80 5 96.05 Repeatability Level(1-1)
1.08201 20 5 1.354 5.01 5 100.11 Repeatability Level(1-2)
1.07321 20 5 1.380 5.14 5 102.87 Repeatability Level(1-3)
1.09784 20 5 1.370 4.99 5 99.83 Repeatability Level(1-4)
1.05551 20 5 1.370 5.19 5 103.84 Repeatability Level(1-5)
1.09365 20 5 1.370 5.01 5 100.21 Repeatability Level(1-6)
Avg 5.02 100.49
SD 0.14 2.73
%RSD 2.71 9.9
1.00314 20 5 0.48 1.91 2.5 76.40 Repeatability Level(2-1)
1.00854 20 5 0.51 2.03 2.5 81.07 Repeatability Level(2-2)
1.00431 20 5 0.53 2.09 2.5 83.64 Repeatability Level(2-3)
1.00351 20 5 0.52 2.08 2.5 83.07 Repeatability Level(2-4)
1.00651 20 5 0.53 2.09 2.5 83.46 Repeatability Level(2-5)
1.00764 20 5 0.52 2.05 2.5 81.93 Repeatability Level(2-6)
Avg 2.04 81.59
SD 0.07 2.73
%RSD 3.34 3.58
1.00026 20 5 0.21 0.86 1 85.58 Repeatability Level(3-1)
1.00131 20 5 0.22 0.87 1 86.69 Repeatability Level(3-2)
1.00013 20 5 0.21 0.85 1 85.19 Repeatability Level(3-3)
1.05241 20 5 0.22 0.84 1 83.62 Repeatability Level(3-4)
1.00074 20 5 0.22 0.88 1 87.54 Repeatability Level(3-5)
1.00163 20 5 0.21 0.84 1 83.86 Repeatability Level(3-6)
Avg 0.85 85.41
SD 0.02 1.54
%RSD 1.80 3.9

Analysed By Verified By
 Reproducbility -(Beer)

Concentration Area
0.50 35.72
1.00 66.72
2.50 150.68
5.00 285.75
10.0 573.66

HYPERICIN
700
600
f(x) = 56.3684245439469 x + 8.30598673300162
500 R² = 0.999848558282908
400
Area

300
200
100
0
0.00 2.00 4.00 6.00 8.00 10.00 12.00
Concentration

Analysed By Verified By
 Reproducbility Hypericin(Beer)
Sample weight Make up volume Volume taken Concentration Result Spike Con. Recoevry REPRODUCIBILITY LEVELS
1.09124 20 5 1.38 5.04 5 100.88 REPRODUCIBILITY LEVEL(1-1)
1.08631 20 5 1.31 4.83 5 96.69 REPRODUCIBILITY LEVEL(1-2)
1.06854 20 5 1.32 4.94 5 98.83 REPRODUCIBILITY LEVEL(1-3)
1.09885 20 5 1.32 4.79 5 95.74 REPRODUCIBILITY LEVEL(1-4)
1.06961 20 5 1.32 4.94 5 98.73 REPRODUCIBILITY LEVEL(1-5)
1.07805 20 5 1.38 5.11 5 102.11 REPRODUCIBILITY LEVEL(1-6)
Avg 4.94 98.83
SD 0.12 2.41
%RSD 2.44 2.44
1.00314 20 5 0.532 2.12 2.5 84.85 REPRODUCIBILITY LEVEL(2-1)
1.00131 20 5 0.525 2.10 2.5 83.89 REPRODUCIBILITY LEVEL(2-2)
1.01984 20 5 0.541 2.12 2.5 84.88 REPRODUCIBILITY LEVEL(2-3)
1.02325 20 5 0.530 2.07 2.5 82.87 REPRODUCIBILITY LEVEL(2-4)
1.00961 20 5 0.515 2.04 2.5 81.62 REPRODUCIBILITY LEVEL(2-5)
1.00895 20 5 0.526 2.09 2.5 83.41 REPRODUCIBILITY LEVEL(2-6)
Avg 2.09 83.59
SD 0.03 1.25
%RSD 1.49 1.49
1.00321 20 5 0.21 0.84 1 83.73 REPRODUCIBILITY LEVEL(3-1)
1.00207 20 5 0.21 0.84 1 83.83 REPRODUCIBILITY LEVEL(3-2)
1.00114 20 5 0.22 0.88 1 87.90 REPRODUCIBILITY LEVEL(3-3)
1.00063 20 5 0.21 0.84 1 83.95 REPRODUCIBILITY LEVEL(3-4)
1.00066 20 5 0.226 0.90 1 90.34 REPRODUCIBILITY LEVEL(3-5)
1.00251 20 5 0.207 0.83 1 82.59 REPRODUCIBILITY LEVEL(3-6)
AVG 0.85 85.39
SD 0.03 3.03
%RSD 3.55 3.55

Analysed By Verified By
 ROBUSTNESS HYPERICIN
ROBUSTNESS Sample weight Make up volume Volume taken Concentration Result Spike Con. Recoevry CHANGES
ROBUSTNESS(1-1) 1.00854 20 5 0.550 2.18 2.5 87.25 EVAPORATION
ROBUSTNESS(1-1) 1.00225 20 5 0.530 2.12 2.5 84.61 TEMPERATURE
ROBUSTNESS(1-1) 1.00125 20 5 0.519 2.07 2.5 82.94
ROBUSTNESS(1-1) 1.00158 20 5 0.536 2.14 2.5 85.62 CENTRIFUGE TIME
ROBUSTNESS(1-1) 1.00254 20 5 0.513 2.05 2.5 81.87
ROBUSTNESS(1-1) 1.00321 20 5 0.540 2.15 2.5 86.12 COLOUM TEMPERATURE
Avg 2.12 84.74
SD 0.05 2.03
%RSD 2.18 2.39

Analysed By Verified By