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Document 9
Document 9
25 (2023) pp
© (2023) Sekolah Pascasarjana Universitas Airlangga, Indonesia
* 1 e- mail : desiadriyantininataebenu@gmail.com _
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Abstrak
Cara agar terhindar dari radiasi sinar UV ialah dengan memakai lotion tabir surya. Tanaman kelor
(Moringa oleifera L.) mengandung 46 jenis antioksidan dan 36 senyawa anti-inflamasi yang berpotensi sebagai
tabir surya. Tujuan dari penelitian ini ialah agar dapat mengetahui pengaruh jenis konsentrasi ekstrak daun kelor
bagi nilai SPF, untuk menganalisis kualitas lotion yang di hasilkan dan tingkat kesukaan probandus terhadap lotion
tabir surya. Penelitian dilakukan di Laboratorium Fisiologi Tumbuhan FMIPA Unud, Laboratorium Kimia Analisis
dan Analisis Farmasi FMIPA Unud, dan Laboratorium Penelitian Terpadu Unud. Rancangan yang digunakan
dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) dengan menggunakan ekstrak daun kelor konsentrasi
0%; 0,5%; 1%; 1,5% dan 2%. Parameter yang ujikan antara lain; uji organoleptik, homogenitas, pH, viskositas,
hedonik serta penentuan angka SPF dengan teknik in vitro. Hasil dari penelitian memperlihatkan jika pengujian pH,
homogenitas dan viskositas F0, F1, F2, F3 dan F4 telah memenuhi syarat SNI. Nilai SPF dari masing-masing
formula adalah 5,6780; 9,7605; 19,0299; 38,0369; 68,8832. Analisis statistik pH dan viskositas memperlihatkan
bahwa adanya perbedaan variasi di setiap formulasi. Analisis statistik uji hedonik memperlihatkan tidak adanya
perbedaan variasi di setiap formulasi. Seiring meningkatnya penambahan ekstrak daun kelor lotion tabir surya maka
semakin tinggi angka SPF.
Kata kunci: kelor, lotion, tabir surya, nilai sun protection factor (SPF)
Abstract
The way to avoid UV radiation is to use sunscreen lotion. Moringa plant (Moringa oleifera L.) contains 46
types of antioxidants and 36 anti-inflammatory compounds that have the potential as sunscreens. The purpose of this
study was to determine the effect of the type of concentration of Moringa leaf extract on the SPF value, to analyze
the quality of the lotion produced and the level of probandus preference for sunscreen lotions. The research was
conducted at the Laboratory of Plant Physiology, FMIPA Unud, Laboratory of Chemical Analysis and
Pharmaceutical Analysis, FMIPA Unud, and the Integrated Research Laboratory of Unud. The design used in this
study was a completely randomized design (CRD) using 0% concentration of Moringa leaf extract; 0.5%; 1%; 1.5%
and 2%. Parameters tested include; organoleptic test, homogeneity, pH, viscosity, hedonic and the determination of
SPF number with in vitro technique. The results of the study show that the pH, homogeneity and viscosity tests of
F0, F1, F2, F3 and F4 have met the requirements of SNI. The SPF value of each formula is 5.6780; 9.7605; 19.0299;
38.0369; 68.8832. Statistical analysis of pH and viscosity showed that there were different variations in each
formulation. Statistical analysis of the hedonic test showed that there was no difference in variation in each
formulation. As the addition of moringa leaf extract to sunscreen lotion, the higher the SPF number.
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JBP Vol. 25, No. 2, Desember 2023- Desi Adriyanti Nina Taebenu , Ni Luh Arpiwi , Ni Wayan
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DOI 10.20473/jbp.v25i2.2023.137-145
Jurnal Biosains Pascasarjana Vol. 25 (2023) pp
© (2023) Sekolah Pascasarjana Universitas Airlangga, Indonesia
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© (2023) Sekolah Pascasarjana Universitas Airlangga, Indonesia
organoleptic test is carried out optically by included liquid paraffin, lexemul, cetyl alcohol,
observing the texture, aroma, color and dimethicone and lanolin. The water phase
impression on the skin . The homogeneity test includes glycerin , TEA, and distilled water. The
was carried out by coating one gram of lotion on water and oil phases were brought together at a
the object glass and then affixing it with another temperature ( ± 65°C) while stirring was carried
object glass , repeating three times for each out thus forming a homogeneous mixture .
formulation . The pH test was carried out using Nipagin and Moringa leaf extract, which are the
a pH meter, replication was carried out 3 times cooling phase, are added to the lotion when the
at each sample concentration. Viscosity test was temperature reaches 40 ° C. In each formula, the
measured using a Brookfield viscometer model concentration of Moringa extract varies by 0%;
DVE spindle number 0 2 with an acceleration of 0.5 % ; 1%; 1.5% and 2%.
100 rpm, three repetitions for each si formula . Table 1. Formulation of Sunscreen Body Lotion
Test to determine the SPF value of the lotion preparations.
using in vitro uv - vis spectrophotometer .
Absorbance readings at wavelengths from 290 Raw material formulation
to 320 nm, each one wavelength can be repeated F0 F1 F2 F3 F4
three times so that 21 repetitions of 7 Liquid Paraffin 3 3 3 3 3
wavelengths are obtained at each sample Lexemul 3 3 3 3 3
concentration . Cetyl alcohol 2 2 2 2 2
Dimethicone 3 3 3 3 3
2.3 Preparation of Moringa leaf extract lanolin 2 2 2 2 2
Glycerin 3 3 3 3 3
Moringa leaves freshly picked from
TEA 1,2 1,2 1,2 1,2 1,2
The tree is washed with water , drained and then Methylparaben 0.1 0.1 0.1 0.1 0.1
weighed to obtain the wet weight and dried until Moringa leaf - 0.5% 1 % 1.5% 2%
the weight is constant. The sample was pureed extract
using a blender, then weighed in order to Aquades add 100 100 100 100 100
determine the dry weight. Maceration was
carried out with 96% ethanol solvent at a sample 2.5 Testing the Physical Properties of Lotion
and ethanol ratio of 1:10 for 5 days and while a. Organoleptic Test
stirring occasionally. Mesarat was filtered using Organoleptic checking is carried out by
filter paper then concentrated at 40 °C with a observing optically by examining the color,
vacuum device rotary evaporator to get crude texture, aroma and effect on the skin of the
extract. Yield is calculated by the formula lotion preparation.
𝐵𝑜𝑏𝑜𝑡 𝐸𝑘𝑠𝑡𝑟𝑎𝑘
% Yield =𝐵𝑜𝑏𝑜𝑡 𝐴𝑤𝑎𝑙 𝑆𝑖𝑚𝑝𝑙𝑖𝑠𝑖𝑎 𝑥 100% b. Homogeneity test
Homogeneity check is carried out by method
coat One gr lotion on
2.4 Making Lotion Preparations Sunblock
lotion preparation formulation is glass object is then affixed with other glass
presented in table 1 which consists of three objects . Observations were made visually for
phases ( water, oil, and cooling ) . The oil and the presence or absence of coarse particles.
water phases were placed in separate beakers Repeat three times for each formula.
and melted on a hotplate at 65 ° C. The oil phase
c. pH test DV-E using spindle number 2 at a speed of 100
Checking the pH was measured using a pH rpm. Repeat three times for each formula.
meter, repeating 3 times for each formula . e. In vitro test value of sun Protection factor
d. Viscosity test (spf)
Checking the viscosity of the lotion was As much as 0.5 g of lotion was dissolved
measured using a Brookfield viscometer model little by little with ethanol pa (Pro Analysis) then
put into a 10 mL volumetric flask along with
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ethanol then sonified for 5 minutes. Liquid in The hedonic test was carried out involving 30
filter using filter paper and pour to the cuvette. probands to determine their level of preference
Read the absorbance using a uv vis for sunscreen lotion . The probandus was asked
spectrophotometer from a wavelength of 290 to to compare the five formulas based on terms of
320 n anometer . texture, color, aroma, impression on the skin and
The SPF value of the lotion is calculated using the presence or absence of irritation. The
the formula: assessment category consists of 3 levels, namely
dislike , like, and really like.
SPF = CF x ∑320
290 EE (λ) X l (λ) X abs (λ)
2.6 Data Analysis
Information: Quantitative data obtained is analyzed by
CF = Correction factors ( 10) ANOVA Analysis of Variance , if different data
EE = Erythemal effect spectrum is obtained α˂0.05 will be followed by Post Hoc
l = Solar intensity spectrum Duncan Test.
Abs = Absorbance of sunscreen products
The value of EE x I is a constant which is
3. RESEARCH RESULTS
presented in table 2.
3.1 Extraction of Moringa Leaves
From 2 kg of fresh moringa leaves used,
Table 2. The Normalied Products function is after experiencing the drying process to produce
used in the SPF calculation.
600 grams of dry powder, then concentrated
No Wavelength (λ nm) EE Xl
through a vacuum rotary evaporator and
1. 290 0.0150
2. 295 0.0817
obtained 47.17 grams of viscous extract with a
3. 300 0.2874 yield of 7.861%.
4. 305 0.3278
5. 310 0.1864 3.2 Organoleptic test
6. 315 0.0839 Organoleptic test was carried out in order
7. 320 0.0180 to know character lotions through optical
Total 1 viewing to see the color, texture, fragrance and
impression on the skin ( Table 3).
Table 3. Organoleptic test results on body lotion
sunscreen preparations from Moringa leaf
f. Hedonic Test extract.
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© (2023) Sekolah Pascasarjana Universitas Airlangga, Indonesia
Homogeneity pH Viscosity
F0 (no EDK) Homogeneous 5.53 ± 0.3215 a 2,000 ± 0.577 a
F1 (EDK 0.5%) Homogeneous 5.48 ± 3.7242 a 2.069 ± 59, 475 b
F2 (EDK 1%) Homogeneous 6.80 ± 0.0577 b 2,377 ± 5,774 c
F3 (EDK 1.5%) Homogeneous 6.21 ± 5.6871 a 2,410 ± 17,321 cd
F4 (EDK 2%) Homogeneous 5.37 ± 4.5622 a 2.440 ± 0.000 s
Information:
EDK: Moringa leaf extract
F0: formulation 0 without Moringa leaf extract
F1: formulation 1 with 0.5 % Moringa leaf extract
F2: formulation 2 with 1% Moringa leaf extract
F3: formulation 3 with 1.5 % Moringa leaf extract
F4: formulation 4 with 2% Moringa leaf extract
Letters a, b, c, cd, d in the column based on Duncan's Post Hoc test the results obtained were significantly different between
formulations F0, F1, F2, F3 and F4.
3.4 Test in vitro value of Sun Protection Factor 190 minutes. F3 with the addition of 1.5%
(SPF) Moringa leaf extract has an SPF value of
The results of testing with techniques in 38.0369 and can provide resistance for 380
vitro sunscreen body lotion is presented in table minutes. F4 with the addition of 2% Moringa
5. Lotion F0 ( without Moringa leaf extract) has leaf extract has an SPF value of 68.8832 and can
an SPF value of 5.6780 which is classified as provide resistance for 688 minutes as presented
moderate protection and can provide resistance in table 5.
for 56 minutes. F1 with the addition of 0.5% Table 5. The results of the SPF value of
Moringa leaf extract has an SPF value of 9.7605 sunscreen body lotion preparations from
classified as maximum protection and can Moringa leaf extract.
provide resistance for 97 minutes. F2 with the
addition of 1% Moringa leaf extract has an SPF formulation SPF protection type
value of 19.0299 and can provide resistance for F0 5.6780 Currently
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formulation which causes the lotion to be
soft, not greasy and easy to apply to the skin
4. DISCUSSION (Maria and Anis, 2021).
The extract obtained weighed 47.17 Homogeneity testing aims to
grams and the yield was calculated and the understand the homogeneity aspect of
yield yield was 7.861% . Determination of sunscreen lotion . Mixed lotion produces
yield plays a role in order to know the dose good quality because it can prove that the
of secondary metabolites carried by the ingredients used in the manufacturing
solvent but does not determine the type of process are perfectly distributed. From
compound (Via and Iman, 2020). research that has been done on lotion
Assessment of the organoleptic properties of preparations F0, F1, F2, F3, and F4 all look
sunscreen body lotion preparations can homogeneous (Jufri et al., 2006).
include color, texture , aroma and effects on The pH measurement aims to
the skin as shown in Table 3 (Ningrum et al, determine the degree of acidity in a sample
2017). Color is the appearance of the body of sunscreen lotion so as not to cause
lotion sunscreen that is observed by the sense irritation to the skin. Lotions that have a pH
of sight. F0 (without Moringa leaf extract) value that is too alkaline make the skin dry,
white body lotion preparation, this is while lotions with a pH value that are too
because it only contains a base without the acidic will irritate the skin (Barel et al.,
addition of Moringa leaf extract. F1 ( 0.5 % 2001). An increase in the concentration of
moringa leaf extract ) was light green, F2 the active ingredients causes a decrease in
(1.5% moringa extract and moringa leaf pH due to the presence of phenolic content
extract) and F3 (1.5% moringa leaf extract) which decomposes into polyphenolic
were green, and F4 (2% moringa leaf extract) compounds contained in the active
was dark green. The more interpolation of ingredients of Moringa. The decomposition
extract variations , the color that appears to that occurs affects the increase in H + as the
be getting darker, this is because Moringa active ingredients are added so that the pH
leaves contain chlorophyll (Sumenda et al., decreases (Ulandari and Sugihartini, 2020).
2011). The next organoleptic parameter is Based on the ANOVA test, it shows that
texture. Texture comes from friction trapped there are differences in variations in each
on the surface of the skin. In liquid textured formulation.
body lotion preparations , this is because it Based on checking the viscosity of
contains more water and has a low oil content sunscreen body lotion from Moringa leaf
in the manufacturing process (Benjamin et extract, the average successive viscosity was
al., 2016). The next organoleptic parameter 2,000; 2069; 2,377; 2.410 and 2.440 as
is aroma. Aroma is a stimulus that is shown in table 5. The viscosity test results
produced from body lotion which is known obtained are in accordance with SNI 16 4399
by using the sense of smell. The sense of 1996 namely 2 . 000-50,000 cps. This can
smell is the instrument that plays the most prove that the higher the level of active
role in knowing the aroma of a product. The ingredients added, the thicker the preparation
distinctive aroma of moringa in body lotion and the viscosity value obtained is higher
preparations F1, F2, F3 and F4 is influenced (Zulkarnain, 2013). Based on the one way
by the addition of moringa leaf extract (Ilona ANOVA test , it shows that there are
and Rita, 2015). The last organoleptic differences in variations in each formulation.
parameter is the impression on the skin after
use. Body lotions F0, F1, F2, F3 and F4 are The effectiveness of a sunscreen
soft, non-sticky, spread easily when applied product is determined based on the
to the skin . This is due to the addition of determination of the SPF. In F0 there is no
liquid paraffin and dimethicone in the Moringa leaf extract, but it has an SPF of
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5.6780. This is due to the fact that when terms of aroma, texture and impression on
making the lotion, it contains lanolin, a the skin. In terms of color, probandus
natural compound found in sheep's wool , so preferred lotion with an extract
it can block free radicals that damage the skin concentration of 0.5 %.
. F1 with the addition of 0.5 % Moringa leaf
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© (2023) Sekolah Pascasarjana Universitas Airlangga, Indonesia
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