* MACCONKEY AGAR :

& differential
mediaa

blu
differentiate
gimnegative
bat

enteric /Ecli a
salmonel

↳
,

Bile Salt/Crystal violet inhibit Que

gram
to

↳ Lactose
for lactose fermentation

I
->

↓
lactic acid

. PH indicator-fum red/pink
.

* BLOOD AGAR :-
↳ contains
differentiate hemolysis
↓
sheep agas (45)) - to

a
o
enriched
↓
media (Vitamin NADP)
,

↳-hemolysi -greenColonies
no
change
.
7

strepto gallolytics
fastidious
S
organism . .

j . ,

* CHOCOLATE AGAR :

↳
Agar
heated
Blood at 75c

RBC their content

lyse released
-

↳ Ex Nemophils
: neisseria
↳ Colonies-grey dark-brown
,

, ,
fan
 GRAM Que
-
-

Cocci Bacille
· ↳ ↳
Staphylococcus Streptococcus ·
Clostridium

- ↓ ·
Listeria

an
Coagulase Que Coagulase Ove Corgnebacterium

empi cokid
·

↓ · Bana

s aureus s epidermidis
·

Actinomyces
Nocardia
s saprophyticus
·

Mycobacterium
·

I
ococcus U
L
I
gallolytics

GRAM OM
---
Locie Rock

↓ ↓

·
Neisseric obada
-

Hemophithes
Moraxella catarrhalis &
Cocobacilli -
-
Bortedella
-
E
others Brucella

·
H -

pylori
Campylobacter
Vibrio
Pseudomonas ·
Enterobacteria
legionella ↳ Escherichia
Bartonella Klebsiella

Shigella
Salmonella

Yersinia
Proteus
 * Culture
new
:
-

----
Based on
consistency Based on
growth detection
i ---
① solid Conventional culture media Automated Can .

↳
② Liquid (Broth) -
③ semi-solid ① Simple / Basal media
Enriched media
for blood/sterilefluid
②
③ Enrichment broth
④ selective media

③ differential media
media

④ transport
④ anaerobic media

④ Ba
Media i
fastidious bact
i)
can
or
a
↳ (A) Peplone
-

1%
Peptone + 0 5% Nad1
.

+ HO

NetentBroth
Pome wate + 2% mea extae

BactGrowth
asse
ntient broth + 1%
agar
(B) id medium- + 0:2-0 5% .

agar
& to check motility
& to maintain stock culture

②
Enriched Media Additional Nutris

L
Media + e

! (Blood egg
,
serum ,

↓
non-fastidious +
fastidious organism
.
(A) Blood Agai
IB) Chocolate
Agar
Loeffler's serum slope
(D) Blood Culture media