Sustainable water treatment :

innovative technologies 1st Edition

Noor
Visit to download the full and correct content document:
https://textbookfull.com/product/sustainable-water-treatment-innovative-technologies-
1st-edition-noor/
More products digital (pdf, epub, mobi) instant
download maybe you interests ...

Innovative Technologies for the Treatment of Industrial

Wastewater A Sustainable Approach 1st Edition Shirish
H. Sonawane

https://textbookfull.com/product/innovative-technologies-for-the-
treatment-of-industrial-wastewater-a-sustainable-approach-1st-
edition-shirish-h-sonawane/

Emerging Membrane Technology for Sustainable Water

Treatment 1st Edition Hankins

https://textbookfull.com/product/emerging-membrane-technology-
for-sustainable-water-treatment-1st-edition-hankins/

Sustainable Solutions for Urban Water Security

Innovative Studies Binaya Kumar Mishra

https://textbookfull.com/product/sustainable-solutions-for-urban-
water-security-innovative-studies-binaya-kumar-mishra/

Smart Water Technologies and Techniques Data Capture

and Analysis for Sustainable Water Management 1st
Edition David A. Lloyd Owen

https://textbookfull.com/product/smart-water-technologies-and-
techniques-data-capture-and-analysis-for-sustainable-water-
management-1st-edition-david-a-lloyd-owen/
Polysaccharides as a Green and Sustainable Resources
for Water and Wastewater Treatment 1st Edition Nurudeen
A. Oladoja

https://textbookfull.com/product/polysaccharides-as-a-green-and-
sustainable-resources-for-water-and-wastewater-treatment-1st-
edition-nurudeen-a-oladoja/

Water Chemistry Analysis and Treatment Pollutants

Microbial Contaminants Water and Wastewater Treatment
De Gruyter Textbook 1st Edition Elzagheid

https://textbookfull.com/product/water-chemistry-analysis-and-
treatment-pollutants-microbial-contaminants-water-and-wastewater-
treatment-de-gruyter-textbook-1st-edition-elzagheid/

Water Conservation and Wastewater Treatment in BRICS

Nations: Technologies, Challenges, Strategies and
Policies 1st Edition Pardeep Singh (Editor)

https://textbookfull.com/product/water-conservation-and-
wastewater-treatment-in-brics-nations-technologies-challenges-
strategies-and-policies-1st-edition-pardeep-singh-editor/

Water Engineering hydraulics distribution and treatment

1st Edition Shammas

https://textbookfull.com/product/water-engineering-hydraulics-
distribution-and-treatment-1st-edition-shammas/

Industrial Water Treatment Process Technology 1st

Edition Parimal Pal

https://textbookfull.com/product/industrial-water-treatment-
process-technology-1st-edition-parimal-pal/
Sustainable Water
Treatment
Innovative Technologies
Sustainable Water
Treatment
Innovative Technologies

Edited by
Zainura Zainon Noor and
Noor Salehan Mohammad Sabli
CRC Press
Taylor & Francis Group
6000 Broken Sound Parkway NW, Suite 300
Boca Raton, FL 33487-2742

© 2017 by Taylor & Francis Group, LLC

CRC Press is an imprint of Taylor & Francis Group, an Informa business

No claim to original U.S. Government works

Printed on acid-free paper

International Standard Book Number-13: 978-1-138-03324-5 (Hardback)

This book contains information obtained from authentic and highly regarded sources. Reasonable efforts
have been made to publish reliable data and information, but the author and publisher cannot assume
responsibility for the validity of all materials or the consequences of their use. The authors and publishers
have attempted to trace the copyright holders of all material reproduced in this publication and apolo-
gize to copyright holders if permission to publish in this form has not been obtained. If any copyright
material has not been acknowledged please write and let us know so we may rectify in any future reprint.

Except as permitted under U.S. Copyright Law, no part of this book may be reprinted, reproduced, trans-
mitted, or utilized in any form by any electronic, mechanical, or other means, now known or hereafter
invented, including photocopying, microfilming, and recording, or in any information storage or retrieval
system, without written permission from the publishers.

For permission to photocopy or use material electronically from this work, please access www.copy-
right.com (http://www.copyright.com/) or contact the Copyright Clearance Center, Inc. (CCC), 222
Rosewood Drive, Danvers, MA 01923, 978-750-8400. CCC is a not-for-profit organization that provides
licenses and registration for a variety of users. For organizations that have been granted a photocopy
license by the CCC, a separate system of payment has been arranged.

Trademark Notice: Product or corporate names may be trademarks or registered trademarks, and
are used only for identification and explanation without intent to infringe.

Library of Congress Cataloging-in-Publication Data

Names: Noor, Zainura Zainon, editor. | Sabli, Noor Salehan Mohammad, editor.
Title: Sustainable water treatment : innovative technologies / [edited by] Zainura
Zainon Noor; Noor Salehan Mohammad Noor.
Description: Taylor & Francis, a CRC title, part of the Taylor & Francis imprint, a
member of the Taylor & Francis Group, the academic division of T&F Informa,
plc, [2017].
Identifiers: LCCN 2016037195| ISBN 9781138033245 | ISBN 9781138033252
(eISBN)
Subjects: LCSH: Water--Purification--Technological innovations. | Sustainable
chemistry.
Classification: LCC TD430 .S84 2017 | DDC 628.1/620286--dc23
LC record available at https://lccn.loc.gov/2016037195

Visit the Taylor & Francis Web site at

http://www.taylorandfrancis.com

and the CRC Press Web site at

http://www.crcpress.com
Contents

Preface..................................................................................................................... vii
Acknowledgments..................................................................................................ix
Editors.......................................................................................................................xi
Contributors.......................................................................................................... xiii

Section I Innovative Biological Processes

for the Recovery of Value-Added
Products from Wastewater
1. Enzymatic Hydrolysis of Waste Cooking Palm Oil by
PVA–Alginate–Sulfate Immobilized Lipase.............................................3
Nor Badzilah Hasan, Tan Wei Yie, and Nor Azimah Mohd Zain

2. Bioremediation of Palm Oil Mill Effluent for Itaconic Acid

Production by Aspergillus terreus NRRL 1960 Immobilized
in PVA–Alginate–Sulfate Beads................................................................. 25
Qistina Ahmad Kamal and Nor Azimah Mohd Zain

3. Optimization of Lipid Content in Microalgae Biomass Using

Diluted Palm Oil Mill Effluent by Varying Nutrient Ration............... 45
Noor Amirah Abdul Aziz, Shreeshivadasan Chelliapan,
Mohanadoss Ponraj, Mohd Badruddin Mohd Yusof,
and Mohd. Fadhil Md Din

Section II MBR Technologies

4. Removal of Micro-Pollutants from Wastewater through MBR

Technologies: A Case Study on Spent Caustic Wastewater.................. 67
Noor Sabrina Ahmad Mutamim and Zainura Zainon Noor

5. The Outlook on Future MBR Technologies............................................. 81

Rabialtu Sulihah Ibrahim, Adhi Yuniarto, and Siti Nurhayati Kamaruddin

6. Integration of Membrane Bioreactor with Various Wastewater

Treatment Systems........................................................................................ 93
Chin Hong Neoh, Zainura Zainon Noor, Cindy Lee Ik Sing,
Florianna Lendai Michael Mulok, and Noor Salehan Mohammad Sabli

v
vi Contents

Section III Advanced Chemical-Physical Processes

for Industrial Wastewater Treatment

7. Wet Air Oxidation Processes: A Pretreatment to Enhance

the Biodegrability of Pharmaceutical Wastewater............................... 113
Ee Ling Yong

8. Application of Nonthermal Plasma in the Treatment of Volatile

Organic Compounds from Wastewater................................................... 123
Abdullahi Mohammed Evuti, Mohd Ariffin Abu Hassan,
Zainura Zainon Noor, and Raja Kamarulzaman Raja Ibrahim

9. Removal of Color Wastewater Using Low-Cost Adsorbent: A

Comparative Study...................................................................................... 141
Venmathy Samanaseh, Mohd Ariffin Abu Hassan,
and Zainura Zainon Noor

10. Bioparticle Development in Constructed Wetland for Domestic

Wastewater.................................................................................................... 157
Mohd. Fadhil Md Din, Zaharah Ibrahim, Zaiton Abd Majid,
Chi Kim Lim, and Abdul Hadi Abdullah

Index...................................................................................................................... 177
Preface

This book focuses on wastewater treatment with green and innovative

technologies that promote sustainability. It talks about studies conducted
on innovation from existing biological, chemical, and physical processes in
wastewater treatment. It aims to help researchers or related parties that are
interested in implementing wastewater treatment with greener technologies.
Since this book covers all the fundamental processes (biological, physical,
and chemical) in wastewater treatment, it will bring great benefits to readers
as they would gain better understanding of green technologies in wastewa-
ter treatments.

vii
Acknowledgments

First and foremost, all praise be to God for his blessings and guidance for
giving us the inspiration to embark on this project and instilling in all of
us the strength to see that this book becomes a reality. Many people have
contributed to the creation and completion of this book. We would like
to acknowledge with appreciation numerous valuable comments, sugges-
tions, constructive criticisms, and praise from evaluators and reviewers.
Their suggestions have greatly helped us to improve the quality of this
book. We also would like to express our gratitude to all who have helped
in one way or another in the planning, brainstorming, writing, and editing
stages of this book especially our Green Technology Research Group. We
would also like to extend our appreciation to Universiti Teknologi Malaysia
for providing us with the facilities vital to the completion of this book.
Finally, we would like to express our appreciation to our families for their
continuous patience, understanding, and support throughout the prepara-
tion of this book.

ix
Editors

Zainura Zainon Noor is an associate professor of chemical engineering at

Universiti Teknologi Malaysia. She embarked on her career in UTM in 1999
as a research officer in a chemical engineering pilot plant prior to joining
the Faculty of Chemical Engineering and Natural Resources 2 years later.
A well-trained chemical engineer specializing in environmental engineer-
ing, Dr. Zainura is an intrinsically passionate individual driven toward
finding greener and eco-friendly solutions. Through her unremitting inter-
est, years of academic study, as well as conducive research and consulta-
tion activities, Dr. Zainura has established and strengthened her expertise in
green technology, including cleaner production, life cycle assessment (LCA),
water and carbon footprints, greenhouse gas inventory and projection as
well as sustainable development. She is an accomplished project manager
and is currently leading the Green Technology Research Group (Green Tech
RG) at one of UTM’s prominent centers of excellence, the Institute of Water
and Environmental Management (IPASA). Recognizing her expertise in
green technology, in 2009, the Department of Environment (DOE) Malaysia
appointed her to develop the Cleaner Production Module, which was later
used as the training module for the department’s officers from all over
Malaysia. Recently, she was selected by the Malaysian Government (under
the Ministry of Natural Resources and Environment) as a consultant for
the development of the Malaysia Environmental Performance Index (EPI).
She is also an appointed committee member of the Green Technology Focus
Group (sustainable solid waste) under the Ministry of Green Technology,
Energy and Water. Dr. Zainura is also a renowned speaker and has given
talks at numerous seminars, workshops, and short courses at both national
and international levels. Dr. Zainura earned her PhD and MS degrees from
Newcastle University, UK, and BS degree from Vanderbilt University.
Noor Salehan Mohammad Sabli is currently a PhD student in environmen-
tal engineering at the Faculty of Chemical Engineering, Universiti Teknologi
Malaysia (UTM) under the supervision of an associate professor Dr. Zainura
Zainon Noor. Her study is in the field of environmental engineering special-
izing in water footprint. Her research focuses on developing water footprint
framework for calculating water usage through crude palm oil production
from nursery until the mill, together with adapting the life cycle assessment
approach in the framework.

xi
Contributors
Abdul Hadi Abdullah
Department of Environmental
Engineering
Universiti Teknologi Malaysia
Johor, Malaysia
Noor Amirah Abdul Aziz
Centre for Environmental
Sustainability and Water
Security
Universiti Teknologi Malaysia
Johor, Malaysia
Shreeshivadasan Chelliapan
Department of Engineering
Universiti Teknologi Malaysia
Razak School
Kuala Lumpur, Malaysia
Mohd. Fadhil Md Din
Centre for Environmental
Sustainability and Water
Security
Universiti Teknologi Malaysia
Johor, Malaysia
Abdullahi Mohammed Evuti
Faculty of Engineering
University of Abuja
Abuja, Nigeria
Nor Badzilah Hasan
Department of Biosciences and
Health Sciences
Universiti Teknologi Malaysia
Johor, Malaysia
Mohd Ariffin Abu Hassan
Department of Chemical Engineering
Universiti Teknologi Malaysia
Johor, Malaysia
Raja Kamarulzaman Raja Ibrahim
Department of Physics
Universiti Teknologi Malaysia
Johor, Malaysia
Rabialtu Sulihah Ibrahim
Faculty of Chemical and Energy
Engineering
Universiti Teknologi Malaysia
Johor, Malaysia
Zaharah Ibrahim
Faculty of Bioscience and Medical
Engineering
Universiti Teknologi Malaysia
Johor, Malaysia
Qistina Ahmad Kamal
Department of Biosciences and
Health Sciences
Universiti Teknologi Malaysia
Johor, Malaysia
Siti Nurhayati Kamaruddin
Faculty of Chemical and Energy
Engineering
Universiti Teknologi Malaysia
Johor, Malaysia
Chi Kim Lim
Faculty of Technology Management
dan Business
Universiti Tun Hussein Onn Malaysia
Johor, Malaysia
xiii
xiv Contributors

Zaiton Abd Majid Venmathy Samanaseh

Department of Chemistry Faculty of Chemical and Energy
Universiti Teknologi Malaysia Engineering
Johor, Malaysia Universiti Teknologi Malaysia
Johor, Malaysia
Florianna Lendai Michael Mulok
Department of Human Resource Cindy Lee Ik Sing
Development Faculty of Chemical and Energy
Universiti Malaysia Sarawak Engineering
Sarawak, Malaysia Universiti Teknologi Malaysia
Johor, Malaysia
Noor Sabrina Ahmad Mutamim
Universiti Malaysia Pahang Tan Wei Yie
Pahang, Malaysia Department of Biosciences and
Health Sciences
Universiti Teknologi Malaysia
Chin Hong Neoh Johor, Malaysia
Centre for Environmental
Sustainability Water Security
(IPASA) Ee Ling Yong
Universiti Teknologi Malaysia Faculty of Civil Engineering
Johor, Malaysia Universiti Teknologi Malaysia
Johor, Malaysia

Zainura Zainon Noor

Centre for Environmental Adhi Yuniarto
Sustainability Water Security Department of Environmental
(IPASA) Engineering
Universiti Teknologi Malaysia Institut Teknologi Sepuluh
Johor, Malaysia Nopember
Jawa Timur, Indonesia

Mohanadoss Ponraj
Mohd Badruddin Mohd Yusof
Centre for Sustainable Technology
Department of Environmental
and Environment (CSTEN)
Engineering
Universiti Tenaga Nasional
Universiti Teknologi Malaysia
Kajang, Malaysia
Johor, Malaysia

Noor Salehan Mohammad Sabli Nor Azimah Mohd Zain

Faculty of Chemical and Energy Faculty Biosciences and Biomedical
Engineering Engineering
Universiti Teknologi Malaysia Universiti Teknologi Malaysia
Johor, Malaysia Johor, Malaysia
 Section I

Innovative Biological
Processes for the Recovery
of Value-Added Products
from Wastewater
1
Enzymatic Hydrolysis of Waste
Cooking Palm Oil by PVA–Alginate–
Sulfate Immobilized Lipase

Nor Badzilah Hasan, Tan Wei Yie, and Nor Azimah Mohd Zain

CONTENTS
1.1 Introduction.....................................................................................................4
1.2 Waste Cooking Oil..........................................................................................4
1.2.1 Environmental Pollution Cause by WCO........................................ 4
1.2.2 Composition of WCO......................................................................... 5
1.3 Lipases.............................................................................................................. 6
1.3.1 Lipase Sources.....................................................................................7
1.3.2 Lipase from C. rugosa......................................................................... 7
1.3.3 Hydrolysis Reaction of Lipase..........................................................8
1.4 Immobilization Method................................................................................. 9
1.4.1 Advantages of Enzyme Immobilization..........................................9
1.4.2 Polyvinyl Alcohol.............................................................................. 10
1.4.3 PVA–Alginate as Supporting Material.......................................... 11
1.4.4 Advantages of PVA–Alginate Matrix............................................. 11
1.4.5 Drawbacks of PVA and Its Solution............................................... 12
1.5 Case Study..................................................................................................... 13
1.5.1 Materials............................................................................................. 13
1.5.2 Pretreatment of WCO....................................................................... 13
1.5.3 Preparation of Lipase Enzyme Solution........................................ 13
1.5.4 Immobilization of CRL.................................................................... 13
1.5.5 Waste Cooking Palm Oil Hydrolysis............................................. 14
1.5.6 Determination of Degree of Hydrolysis (Conversion)
and Rate of Hydrolysis..................................................................... 14
1.5.7 Fatty Acid Concentration Determination...................................... 14
1.6 Results and Discussions.............................................................................. 15
1.6.1 Effects of Temperature on Immobilized CRL............................... 15
1.6.2 Effects of pH on Immobilized CRL................................................ 16

3
4 Sustainable Water Treatment

1.6.3 Effect of Enzyme Loading on Immobilized CRL......................... 16

1.6.4 Comparative Study of Free and Immobilized CRL..................... 17
1.7 Conclusion..................................................................................................... 18
References................................................................................................................ 19

1.1 Introduction
Fatty acids exist in nature as carboxylic acids with long hydrocarbon chains,
which are either saturated or unsaturated. They consist of carbon (C), hydro-
gen (H), and oxygen (O) and are arranged as a carbon chain skeleton with
a carboxyl group (−COOH) at one end. The hydrocarbon chain length may
vary from 10 to 30 carbons but it is usually from 12 to 18 carbons. Fatty acids
are usually derived from triglycerides and are the main component of vege-
table oil and animal fats. Fatty acids are widely used as raw materials in food,
cosmetics, the pharmaceutical and dairy industries, and skin care products.
Today, the production of fatty acid and glycerol from cooking palm oil is vital
especially in oleochemical industries (Serri et al., 2008). Many researchers
have used enzyme-catalyzed hydrolysis in order to reduce energy consump-
tion and minimize thermal degradation of the products. However, studies
using immobilized lipase which has the ability to hydrolyze cooking palm
oil into fatty acid and glycerol have not been widely explored.
Waste cooking oil (WCO) is known for its high acid value of free fatty acids
(FFAs; Araujo, 1995). FFAs are value-added products because of their wide
industrial applications such as soap production, surfactants manufacturing,
biomedical uses, and biodiesel production (Hill, 2000; Habulin and Knez, 2002).

1.2 Waste Cooking Oil

WCO is the residue from the kitchen, restaurants, and food factories. WCOs
are basically generated from vegetable oils used at high temperature in
food frying. As a result, this process causes hydrolysis, polymerization, and
­oxidation reactions which change the physical and chemical properties of
the oil.

1.2.1 Environmental Pollution Cause by WCO

Increasing production of WCO from household and industrial sources is a
growing issue all around the world. Table 1.1 shows the quantity of WCO
produced in selected countries. This residue usually contains large amounts
of FFAs, polymers, and decomposition products besides triglyceride and
Enzymatic Hydrolysis of Used Cooking Palm Oil 5

TABLE 1.1
Quantity of WCO Produced in Selected Countries
Country Quantity (million tonnes/year)
United States 10.0
China 4.5
European Union 0.7–1.0
Japan 0.45–0.57
Malaysia 0.5
Canada 0.12
Taiwan 0.07

some diglyceride due to the reaction of oxidation and hydrogenation (Lam et

al., 2010). This residue is regularly being poured down the drain resulting in
a wastewater treatment problem. Besides this, the residue can be integrated
into the food chain via animal feed, thus resulting in a potential human
health risk (Costa Neto et al., 2000). Recently, WCO rich in fatty acids has
gained great interest due to its use in biodiesel production.

1.2.2 Composition of WCO

WCOs consist of saturated and unsaturated fatty acid, for instance; waste
cooking palm oil is rich in palmitic acid, oleic acid, linoleic acid, and stearic
acid. The fatty acids that do not have double bonds are termed “saturated,”
such as stearic acid and palmitic acid. These chains contain the maximum
number of possible hydrogen atoms per atom carbon. Fatty acids that have
double bonds are termed “unsaturated,” such as linoleic acid and oleic acid.
These chains do not contain the maximum number of hydrogen atoms due
to the presence of double bond(s) on some carbon atoms (Lam et al., 2010).
WCO and fatty acid compositions are summarized in Table 1.2 (Hingu et al.,
2010) and Table 1.3 (Wan Omar et al., 2009). The analysis in Table 1.3 shows that

TABLE 1.2
WCO Profile
WCO Composition Percentage
Fatty acid 32.13
Ester 42.55
Methyl ester 2.14
Ketone 2.54
Aldehyde 4.69
Alkane 2.16
Alkene 0.68
Alcohol 0.29
Other 11.41
6 Sustainable Water Treatment

TABLE 1.3
FFA Profile in WCO
Fatty Acid Composition Percentage (%)
Palmitic acid C16:0 9.08
Stearic acid C18:0 2.16
Oleic acid C18:1 35.34
Linoleic acid C18:2 53.4

they are mainly composed of 90% of unsaturated fatty acids (­linoleic and oleic
acids) and 10% saturated fatty acids (palmitic and stearic acid).

1.3 Lipases
Lipase enzymes are important in biological systems which belong to the group
of serine hydrolyses (E.C. 3.1.1.3) (Jaeger and Eggert, 2002). They are mostly
found built on an alpha and beta hydrolase fold with beta sheet c­ ontaining
the catalytic residues. Their catalytic site is composed of serine, aspartic acid,
and histidine. The interior topology of alpha and beta hydrolase fold protein
is mainly composed of parallel beta pleated strands ­separated by an alpha
helix. Figure 1.1 shows the 3D structure of lipase from Candida rugosa. In
Figure 1.1, “a” represents the helices which are packed against the central
L-sheet. “b” represents the central L-sheet while “c” represents the smaller

a b a c

FIGURE 1.1
CRL enzyme 3D structure (Adapted from Cygler, M. and Schrag, J. D. 1999. Biochimica et
Biophysica Acta. 1441: 205–214.).
Enzymatic Hydrolysis of Used Cooking Palm Oil 7

N-terminal L-sheet. The closed conformation of the lid is represented by “d”

and the open conformation is represented by “e”. The residues forming the
catalytic triad are indicated by “e”.
Lipases are like esterases. They catalyze the hydrolysis and the transesteri-
fication of ester groups. However, the difference between esterases and lipase
is that esterases act on soluble substrates while lipases catalyze reactions on
water insoluble substrates and the presence of the water or lipid interface is
a usual prerequisite for efficient catalysis (Cygler and Schrag, 1999). Their
natural substrate is triglycerides. Lipases catalyze the hydrolysis of triglycer-
ides to glycerol and fatty acids (Vaidya et al., 2008).
Lipases can also catalyze a wide range of enantio- and regioselective ­reactions
such as hydrolysis, esterification, transesterification, aminolysis, and ammonol-
ysis depending on the nature of substrate and reaction conditions (Vaidya et al.,
2008). The versatility of lipase-catalyzed reactions made them greatly applied
in numerous industrial processes including oils and fats, detergents, baking,
cheese making, hard-surface cleaning as well as leather and paper processing
(Schmidt and Verger, 1998; Jaeger et al., 1999; Villeneuve et al., 2000).

1.3.1 Lipase Sources

Various enzymes that are isolated from 2% of the world’s microorganisms have
been used as enzyme sources (Hasan et al., 2006). However, microbial enzymes
are often more widely used compared to enzymes that are derived from plants
or animals because of the great variety of catalytic activities available, high
yield possibility, ease of genetic manipulation, and rapid growth of microor-
ganisms on inexpensive media. In addition, microbial enzymes production is
more convenient, safer, and exhibits higher stability (Wiseman, 1995).
In recent years, research on microbial productions of lipase has increased
because of their wide application in industry such as the hydrolysis of fats,
production of fatty acids, food additives, detergent additives, cosmetics, and
care products (Bjorkling et al., 1991; Malcata, 1996). Lipases have been i­ solated
from a wide number of plant, animal, and microbial sources (Sangeetha et
al., 2010). Lipase producing microorganisms includes bacteria, fungi, and
yeasts (Rapp, 1995). The ease of isolation of microbes’ lipase has made both
bacteria and fungi predominant sources of lipase. Lipases obtained from
fungal sources were thought to be the best source for commercial application
until bacterial lipases were discovered. Table 1.4 (Hasan et al., 2006) shows a
lipase producing microorganism.

1.3.2 Lipase from C. rugosa

Lipases from C. rugosa were firstly described early in the 1960s by isolating
the yeast from natural soils. It is also known due to its great lipase production
(Yamada et al., 1963; Tomizuka et al., 1966). In addition, the lipases from yeast
were nonpathogenic. Therefore, there were a great number of reviews about
8 Sustainable Water Treatment

TABLE 1.4
Isolation of Lipase from Various Microorganisms
Lipase-Producing
Type of Microorganism Microorganisms Reference
Bacteria Bacillus sp. Imamura and Kitaura (2000)
Bacillus subtilis Ruiz et al. (2005)
Pseudomonas sp. Sarkar et al. (1998)
Pseudomonas aeruginosa Chartrain et al. (1993)
Staphylococcus aureus Gotz et al. (1998)
Penicillium cyclopium Chahinian et al. (2000)
Lactobacillus plantarum Lopes et al. (1999)
Chromobacterium viscosum Taipa et al. (1995)
Fungus Candida cylindracea Muralidhar et al. (2001)
Rhizomucor miehei Herrgard et al. (2000)
Acinetobacter sp. Snellman et al. (2002)
Fusarium solani Knight et al. (2000)

C. rugosa, highlighting its different aspects like biochemical, fermentation

technology, or some biocatalytical applications (Benjamin and Pandey, 1998;
Cygler and Schrag, 1999; Akoh et al., 2004). Furthermore, the lipase produced
by C. rugosa is one of the most commonly used enzymes in organic ­solvents
due to its high activity of hydrolysis, esterification, transesterification, and
aminolysis (Villeneuve et al., 2000). The findings were also proven in the
studies by Winayanuwattikun et al. (2011). In this study, C. rugosa lipase
(CRL) also showed a broader range of substrate specificity with high activity
for the substrates from 4 to 16 carbon chain length (Winayanuwattikun et al.,
2011). Formally, C. rugosa is known as Candida cylindracea (Khor et al., 1985).

1.3.3 Hydrolysis Reaction of Lipase

A lipase reaction system usually consists of two immiscible phases where
the water phase contains dissolved lipase while the organic phase contains
dissolved substrates (triglycerides). The water phase of lipase is contacted
with the triglycerides in organic phase forming liquid–liquid dispersion.
Lipases catalyze the hydrolysis of triglyceride into FFAs and glycerol at the
interface between the two liquids (Murty et al., 2002). Triglycerides do not
dissolve in the water phase, thus the reaction has to be placed at the interface
of the lipid and water phase (Pronk et al., 1988). The reaction often starts with
reversible adsorption of the enzyme at the interface which then binds to the
substrate to initiate catalysis (Tsai and Liching, 1990). Since the hydrolysis
activity occurs at the lipid–water phase, the presence of an organic solvent is
necessary to solubilize the lipid in water in order to achieve their hydrolysis
catalyzed by lipases (Torres and Otero, 1996). On the other hand, emulsifiers
can be added to increase the interface of the lipid–water emulsion. However,
Enzymatic Hydrolysis of Used Cooking Palm Oil 9

O CH2OH
H2C O O
+ CHOH
HC O OH
O
CH2OH
H2C O
Free fatty acid Glycerol
Triglyceride

FIGURE 1.2
Hydrolysis reaction of lipase (Adapted from Gupta et al., 2008. International Journal of Biological
Macromolecules. 42: 145–151.) The hydrolysis reaction yields 1 mole of g ­ lycerol and 3 moles
of fatty acid per mole of triglycerides (Adapted from Murty, V. R., Bhat, J. and Muniswaran,
P. K. A. 2002. Biotechnology and Bioprocess Engineering. 7: 57–66.)

according to Chew et al. (2008), the addition of emulsifiers is not preferred

to avoid additional separation processes which are not favorable in indus-
try. Figure 1.2 explains the hydrolysis reaction by lipase (Gupta et al., 2008;
Villeneuve et al., 2000).

1.4 Immobilization Method

Immobilization can be defined as the technique used for physical or chemi-
cal fixation of cells, organelles, enzymes, or other proteins onto a solid sup-
port, into a solid matrix, or retained by a membrane, in order to increase their
repeated or continuous use (IUPAC). There are three main types of methods
used for immobilizing enzymes, such as entrapment, cross-linking, and
carrier binding as shown in Figure 1.3. The selection of the immobilization
method is based on several factors, such as overall enzymatic activity, cost of
immobilization method, toxicity of immobilization reagents, and the effec-
tiveness of enzyme utilization (Ozturk, 2001). Among various ­techniques
employed for the immobilization of enzymes, entrapment m ­ ethods are
mostly used in cell immobilization procedures. The inert characteristics of
the matrix result in relatively little damage to the native enzyme’s structure.
Besides that, the polymer used was inert to enzymes. Therefore, enzyme
denaturation can be avoided. Furthermore, entrapped enzymes are more
suitable for use with smaller size of substrates as larger enzymes will not be
able to pass through the membrane and reach the active site of the biocatalyst
(Villeneuve et al., 2000).

1.4.1 Advantages of Enzyme Immobilization

Enzymes are widely used as catalysts in many industrial, biomedical,
and analytical processes. There has been considerable interest in enzyme
10 Sustainable Water Treatment

Method of enzyme immobilization

Carrier binding Cross-linking Entrapment

Physical Ionic Covalent Lattice Microcapsule

absorption bonding binding type type

FIGURE 1.3
Methods for enzyme immobilization.

immobilization due to the simple preparation. The use of immobilized enzymes

offers several advantages compared to free enzymes. The first advantage of
­immobilization permits the repeated use of enzymes. Although the immobi-
lization procedure further increases the manufacturing expense, the multiple
use of immobilized enzymes provides cost advantages. An ideal immobiliza-
tion model is one which permits a high turnover rate of the enzyme yet retains
its high catalytic activity over time. Secondly, the repeated use of enzymes
allows continuous processes in industry as immobilized enzymes can be used
immediately after a reaction. Thirdly, many experiments have shown that
immobilized enzymes significantly enhanced stability in terms of thermal
and pH aspects. Immobilization increased the thermal stability of enzymes
that conferred their use for longer periods at a higher temperature compared
to free enzymes. Besides that, immobilization broadened the working pH of an
enzyme. This could allow enzyme activity over a wider pH (Twyman, 2005).
Other than that, it enables the enzyme to be e­ asily ­separated from the prod-
uct. This would simplify enzyme ­applications; ­support a ­reliable and efficient
technology, as well as provide cost advantages (Tischer and Wedekind, 1999).

1.4.2 Polyvinyl Alcohol

Polyvinyl alcohol (PVA) has been used as immobilization matrix since about
14 years ago (Hassan and Peppas, 2000). It is also the largest hydrophilic
Enzymatic Hydrolysis of Used Cooking Palm Oil 11

synthetic polymer produced in the world (Ramaraj, 2000). PVA is rubber

elastic-like in nature, nontoxic, and odorless. PVA is a polymer of great
interest due to its desirable application in the pharmaceutical and biomedi-
cal fields (Hassan and Peppas, 2000). In the biomedical field, PVA has been
proposed as a promising biomaterial that is suitable for tissue mimicking,
vascular cell culturing, and vascular implanting (Cygler and Schrag, 1999).
For instance, PVA has been applied in tissue engineering for regenerating a
wide variety of tissue and organs, including arterial phantoms, heart valves,
corneal implants, and cartilage tissue substitutes.

1.4.3 PVA–Alginate as Supporting Material

Cell entrapment in polymeric matrices is widely used for cell immobilization
(Wu and Wisecarver, 1992; Zhang et al., 2005). It also proves that the immo-
bilization enzyme on polymer matrices lead to better reusability (Idris et al.,
2008). The living cells are enclosed in a polymeric matrix which is porous
enough to allow the diffusion of substrates into the cells and permit prod-
ucts of enzymatic reaction to move away from the cells (Wu and Wisecarver,
1992). A wide variety of materials have been successfully used for cell entrap-
ment such as agar, agarose, kappa carrageenan, collagen, alginates, chitosan,
polyacrylamide, polyurethane, and cellulose (Ariga et al., 1987). Recently,
the use of PVA for immobilization purpose has been investigated (Wu and
Wisecarver, 1992; Hashimoto and Furukawa, 1987). Ariga et al. (1987) used
the freezing and thawing of the PVA method to form a gel suitable for cell
immobilization. They found that this technique produced a low cost mate-
rial and exhibited high strength with rubber-like elasticity (Ariga et al.,
1987). PVA cross-linked with boric acid has been developed by Hashimoto
and Furukawa (1987). They have used a new inexpensive and less energy-
intensive immobilization method to immobilize activated sludge using PVA.
They cross-linked the PVA using boric acid solution and a monodiol type of
PVA–boric acid gel lattice was produced. The activated sludge was success-
fully immobilized in PVA–alginate beads without loss of biological activ-
ity (Hashimoto and Furukawa, 1987). A modified PVA–alginate bead was
developed and reported by Idris et al. (2008) by introducing sodium sulfate.
The beads produced by this technique were found to be more stable in terms
of chemical and mechanical strength. The beads also displayed superior
enzyme activity and showed relative good diffusivities (Zain et al., 2010).
Similar findings were also reported by Takei et al. (2011).

1.4.4 Advantages of PVA–Alginate Matrix

In recent years, the use of PVA for cell immobilization has attracted wide
attention due to several advantages that it offers. PVA is a cheap and nontoxic
synthetic polymer as well as being easy to process. Besides this, enzymes
immobilized in a PVA matrix showed high activity and high stability with
12 Sustainable Water Treatment

repetitive use, thus increasing economic viability of biosynthetic process-

ing (Zain et al., 2010). On the other hand, PVA is a hydrophilic support-
ing ­material. Generally, the use of hydrophilic support in immobilization
enhances enzyme stability while the use of hydrophobic support material
appears to have its disadvantages. PVA can cause protein stabilization by its
attachment to the polymer chains (Kozhukharova et al., 1988). It is ­suitable
for use for immobilization purposes because it can be easily modified
through its hydroxyl groups. PVA also offers assorted advantages over the
conventional alginate matrix including lower production cost, higher robust-
ness, and nontoxicity to viable cells. PVA beads exhibit rubber-like elastic-
ity in nature (Hassan and Peppas, 2000). Thus, PVA beads provide stronger
mechanical strength compared to alginate beads. Moreover, PVA beads
­display high stability within a wide range of pH that is from pH 1 to pH 13,
while alginate beads are relatively stable in the range of 6–9 (Khoo and Ting,
2001). Furthermore, alginate beads encountered a weight loss up to 20% and
24% at low and high pH. By being aware of these advantages, PVA has been
used widely in cell immobilization (Idris et al., 2008).

1.4.5 Drawbacks of PVA and Its Solution

The PVA–boric acid technique provides an easy and low cost method in
enzyme immobilization. However, there are some problems when using the
PVA–boric acid method for immobilization. PVA is a sticky material, thus
PVA beads have the tendency to agglomerate (Wu and Wisecarver, 1992).
Nevertheless, this matrix is still used by many researchers. In order to
­eliminate the agglomeration, calcium alginate has been introduced. It also
serves to improve the surface properties of the beads (Wu and Wisecarver,
1992; Yujian et al., 2006). The application of calcium alginate in a mixture with
PVA for enzyme immobilization has been reported by Wu and Wisecarver
(1992). The PVA–alginates beads produced were proven to be very strong
and durable with no biological loss for 2 weeks of continuous operation in a
fluidized bed reactor.
The introduction of sodium alginate in the PVA–boric acid method was
also suggested by Slokoska et al. (1999). The finding of Slokoska et al. (1999)
demonstrated that the photo-cross-linked PVA and calcium alginates beads
are suitable for the entrapment of fungal cells (Slokoska et al., 1999). PVA
exhibits a high degree of swelling in water (Hassan and Peppas, 2000). It will
readily dissolve in aqueous solution causing the enzyme to leak out from the
matrix (Zain et al., 2011). Therefore, the PVA must cross-link either chemically
or physically to make it soluble. The most popular cross-linking reagent for
immobilization is glutaraldehyde (Villeneuve et al., 2000). Besides that, cross-
linking the PVA using boric acid solution to produce a monodiol type PVA–
boric acid gel lattice has also been reported by Hashimoto and Furukawa
and Wu and Wisecarver. The other drawback of this polymer is that the
saturated boric acid solution is highly acidic (pH < 4) and it causes a drastic
Enzymatic Hydrolysis of Used Cooking Palm Oil 13

decrease in the viability of immobilized cells. This obstacle can be overcome

by adding sodium sulfate which acts as an inducer for cross-­linkage of PVA
to avoid the drastic decrease in cell viability caused by s­ aturated boric acid
solution (Idris et al., 2008; Takei et al., 2011).

1.5 Case Study

1.5.1 Materials
CRL (3.1.1.3) (Type 1176 U/mg) was purchased from Sigma Aldrich (Japan).
PVA 60,000 MW and boric acid were purchased from Merck Schuchardt OHG,
Darmstadt, Germany. Sodium alginate was obtained from FlukaChemie
GmbH, Buchs, sodium sulfate from GCE Laboratory Chemicals, and calcium
chloride from R&M Marketing, Essex, UK. Iso-octane with 99.84% assay was
purchased from Fisher Chemicals (UK). Other reagents used were analytical
reagent grades and used without further purification including phosphate
buffer solution pH 7.5 or otherwise stated.

1.5.2 Pretreatment of WCO

WCO was obtained from a food stall near Universiti Teknologi Malaysia
(UTM). For a successful reaction, the oil must be free from water and other
impurities. Initially the samples of waste cooking palm oil were filtered to
remove any suspended food particles. Then, the waste cooking palm oil was
heated at 105°C for 1 h to remove its water content. After that, the titrimetry
method with NaOH was used to determine the FFA content in the WCO
(Patil et al., 2010).

1.5.3 Preparation of Lipase Enzyme Solution

The pH of the phosphate buffer solution was adjusted to pH 7.5. CRL (5 g)
was dissolved in 100 mL of phosphate buffer solution. Then, the enzyme
solution was filtered using a 0.45 µm nylon syringe filter to sterilize the
enzyme. The sterilized enzyme solution was stored at 4°C until further used.

1.5.4 Immobilization of CRL

CRL solution with the volume of 10 mL, 5% (v/v) was mixed with 90 mL PVA–
alginate solution. The mixture was mixed comprehensively and i­ ntroduced
as drops by using a rotary pump into a 100 mL mixed solution of saturated
boric acid 5% (w/v) and calcium chloride 2% (w/v). The beads were stirred
gently for 30–50 min to complete the solidification. Then, the PVA–alginate
14 Sustainable Water Treatment

beads were stored at 4°C for 24 h. After 24 h, the mix solution of boric acid
and calcium chloride was discarded and replaced with a 7% (v/v) boric acid
solution. The beads were stirred in the boric acid solution for 30 min and the
solution was then replaced with 0.5 M of sodium sulfate solution and stirred
for another 30 min. Then, the beads were kept at 4°C until further used (Idris
et al., 2008).

1.5.5 Waste Cooking Palm Oil Hydrolysis

A conical flask of 250 mL was initially filled with 3 g of cooking palm oil and
30 mL of iso-octane solvent. Phosphate buffer solution (30 mL, pH 7.0; unless
otherwise stated) was added into the conical flask so that the ratio of oil to
aqueous (buffer solution) is 1. The mixture formed two layers. Three other
identical mixtures as above were prepared. To start the reaction, 0.3 g of CRL
was added to three flasks of reaction mixtures and one was left without the
CRL for control measurement. The mixtures were agitated in the orbital
shaker at 45°C at 200 rpm. Samples were withdrawn from the oil every 30
min. The same procedure was carried out using ­immobilized lipase (Serri et
al., 2008). To determine the effect of parameters on the hydrolysis of WCO,
three variables were taken into consideration which is pH, temperature, and
enzyme concentration. The pH varied from 7 to 8. The temperature varied
from 30°C to 50°C and enzyme l­oading varied from 2 to 8 g of beads which
correspond to 96.43 to 385.73 U/mL enzyme.

1.5.6 Determination of Degree of Hydrolysis

(Conversion) and Rate of Hydrolysis
The degree of hydrolysis was determined by titration of the oil phase
­samples with 0.1 M sodium hydroxide (NaOH). To each sample, 5 mL of the
oil phase was dissolved in 5 mL ethanol:diethyl ether (1:1% v/v). The amount
of 0.1 M NaOH required to neutralize the acid was noted. A blank titration
was done as a control sample. Phenolphthalein was used as an indicator.
The degree of hydrolysis, X is calculated as below (Serri et al., 2008).

(ml NaOH used) (molarity of NaOH)

(average molecular weight of fatty acid) (1.1)
X% =
10 (weight of sample)

1.5.7 Fatty Acid Concentration Determination

All samples collected were analyzed using Perkin Elmer Autosystem XL
equipped with a flame-ionization and a Nukol™ 15 m × 0.53 mm i.d. col-
umn coated with 0.5 µm (25326) column. Helium served as a carrier gas at
a flow rate of 20 mL/min. The column temperature was programmed from
110°C to 220°C with the increase of 8°C/min. The injector and detector
Another random document with
no related content on Scribd:
 Virtutis morem non, set mulieris amorem
Querit, et hoc solo temptat arare solo.
Sic honor ex onere non est, nam fulget honore
Corpore, set corpus non digitabit onus.

Hic loquitur de Simonia prelatorum, et

qualiter hii delicati, dicentes se esse ecclesiam,
aliis grauiora imponunt, et vlterius de censura
horribili laicos pro modico impetuose infestant.

Ecce, deo teste, vir qui non intrat ouile

Capm. xii.
Per portam, latro furque notatur eo.
Sic et in ecclesiam promotus per Simoniam
Clerus, furtiuo se gerit inde modo;
Nec bona de furto conferre placencia Cristo
1010 Quis valet, immo deus pellit ab inde manus.
Ergo valet fiscus que non vult carpere Cristus;
Sunt quia mundana, mundus habebit ea:
Namque suo iure dum clerus abutitur, inde
Priuari dignum iura fatentur eum.
Se vocat ecclesiam clerus, quasi diceret, illam
Non tanget laicus, est honor immo suus:
Sic fastus cleri communi iustificari
Non vult iusticia, set latitante via.
Se leuat et reliquos subdit predatque subactos
1020 Legis composite de nouitate sue:
Sic modo sub specie diuina cerno latere
Has pompas mundi, stant neque iura dei.
Sancta quid ecclesia est hominum nisi turma
fidelis?
Sic patet vt laicus, quem colit ipsa fides,
Est pars ecclesie, melior nec clericus ipse,
Ni melius viuat. Quis michi tale negat?
Vna fides, vnum baptisma, deus manet vnus,
Sic nos ecclesia iungit et vna tenet;
Et veluti multa tegit vna cortice grana,
1030 Sic populos plures colligit vna fides.
Ecclesie sancte cur tunc sibi nomen habere
Vult tantum clerus, alter vt ipse deus?
Appendit legis pondus collis alienis,
Set non vult humeris quid graue ferre suis
Omnia dat licita sibimet, michi set prohibenda;
Ille quiescit, ego sudo labore meo.
Sic iter ex factis viciis prebet faciendis,
Verba set econtra dicet in aure tua:
Hinc plebs attonita dubitat, si credere dictis,
1040 An cleri factis debeat ipsa prius.
Set prohibens michi rem, dum sit culpandus
eadem,
Vix credo verba, sunt quia facta rea:
Tollere sicque nouos de clero cerno superbos,
Per veteres humiles quod d e d i t ipsa fides.
Precipiunt isti maxillam percucienti
Subdere, sic vt eo stet pacienter homo:
Intuleris set eis si quid graue, mox tibi mortis
Censuris anime dant maledicta tue.
Qui necat hic animam sub pena mortis, eadem,
1050 Si posset, corpus perderet ipse prius.
Sic magis ipse lupo fert pastor dampna maligno
In iugulando suas, quas medicaret, oues.
Hii gestant celi claues, intrant nec et ipsi,
Nos nec inire sinunt, quos sine lege regunt:
Nec populi mentes doctrine vomere sulcant,
Nec faciunt operis id quod oportet opus.
Ad dextram Cristi vellent residere beati,
Set nollent calicem sumere, Criste, tuam.
Hii piscatores laxant sua recia lucris,
1060 Vt capiant mundum, non animabus opem.
Sic male viuentes laicis exempla ministrant,
Qui velut instructi more sequntur eos:
Sic ouis ex maculis pastoris fit maculosa,
Et cadit in foueam cecus vterque simul.
 Hic loquitur qualiter prelatus non solum
doctrina set eciam bonis actibus populo sibi
commisso lucere deberet.

In tenebris pergens nescit quo vadat, vt ille

Capm. xiii.
Qui non discernit que sit habenda via;
Cumque caret populus doctrina, nec videt ipsum
Qui suus est presul iura tenere dei,
Cum neque scripta docet, neque facta facit
pietatis,
1070 Immo sui vicii dedita culpa patet,
Cum de nocte sua pereat sine luce lucerna,
Et virtutis habent presulis acta nichil,
Tunc errare facit plebem, sine luceque cecus
Cecum consequitur, vnde ruina venit.
Ergo suas luces accendant clarius illi
Qui sunt ductores, vt videamus iter.
Igne lucerna micans tria dat, splendet, calet, vrit;
Hec tria presul habet sub racione trium:
Vita splendorem demons t r a t , a m o r e calorem,
1080 Et quia peccantes arguit, vrit eos.
Cum populum sibi corde ligat, precibusque beatis
Seruat et auget oues, tunc placet ipse deo.
Vt sit sollicitus quicumque pauore tenetur,
Ne lupus ille Sathan intret ouile suum:
Pascat oues presul exemplaque sancta ministret,
Vt sapiant dulces mellis in ore fauos.
Sepius assueuit Tubicen prodesse, suosque
Dux bene pugnantes concitat ore viros:
Te magis, o presul, qui dux es spiritualis,
1090 Promere lege dei consona verba decet.
Solue tuam vocem sicut tuba ductilis altam,
Osque tuum verbis instruat acta gregis:
Clama, ne cesses, populo dic crimen eorum,
Preuius exemplis tu tamen esto bonus.
Dum sapor assidua remanens sit dulcis in vnda,
Gracius ex ipso fonte bibuntur aque:
 Cum magis in Cristo sit cleri vita beata,
Quem docet ille magis, sermo beatus erit.
Sermo dei numquam vacuus redit, immo lucrata
1100 Conferet emissus dupla talenta lucri:
Sermo dei purus, mens quem sincera ministrat,
Claustra poli penetrans dona reportat humo.
Curatos anima tales que possidet egra,
Inueniet, si vult, sana salutis iter.
Qui nil terrenum sapiunt, set celica querunt,
Et solum siciunt esuriuntque deum;
Quos non librarum pascit nitor, immo librorum,
Non facies auri, set cibat ara dei;
Hii, cum sint propria digni mercede laboris,
1110 Permansura serunt que sine fine metunt.
Sic qui recta docet, facit et super hoc quod
oportet,
Expedit vt facias quod tibi dictat opus:
Tunc bene fortis equs reserato carcere currit,
Cum quos pretereat quosque sequetur habet.
Legis enim veteris scripture sunt memorande,
Quo bonus exemplum pastor habere queat.
Commemoranda satis fuit hec sapiencia, quando
Ante gregem virgas ordinat ille Iacob:
Partim nudat eas ablato cortice, partim
1120 Corticis indutas veste relinquit eas.
In virgis splendet sublato cortice candor,
Cum de scripturis splendida verba trahit;
Cortex saluatur, cum litera sola tenetur,
Et pastor sensu simplice pascit oues.393
Set quid pastores dicent exempla negantes?
Vt sibi proficiant ista nec illa tenent.
Cuius nec vita bona seu doctrina iuuabit,
Instruat vt populum, nil reputamus eum.
Indiscreta tamen sunt qui documenta parantes
1130 Scismatis in plebem magna pericla mouent.
Indocti causa doctoris sepe scolares
Virtutis capiunt commoda nulla scole:
 Sic importuni prelati, quamuis habundent
Dogmata, si desint acta, vigore carent.
Quidam corripiunt magis ignibus impetuosi,
Et velut vrsus oues de feritate premunt;
Talis enim doctor, cum durius increpat vllum,
Ledit eum cuius debuit esse salus;
Vulnerat ipse reos, set vulnera nulla medetur;
1140 Prouocat in peius quod fuit ante malum:
Sic nos prelati nequit os curare superbi,
Cum viciosus homo moribus auctor erit.
Est et prelatus, qui corripiens quasi blando
De sermone fauet, nec reus inde cauet:
Corripiebat Hely pueros dulcedine patris,
Non vice pastoris, non grauitate soni;
Pro quorum culpa dampnatur Hely, quia valde
Impius in pueris per pia verba fuit.
Sic pastor qui subiectos non corripit, iram394
1150 Summi patris emens carcere dignus erit.
Ista solent scribi, ‘Medium tenuere beati,
Non nimis alta petas, nec nimis yma geras.’
Non nimis ex duro presul nos iure fatiget,
Nec nimis ex molli simplicitate sinat.
Si non leua manus equitis moderacior extet,
Oppositis frenis sepe repugnat equs.
Eripit interdum, modo dat medicina salutem,
Nil prodest quod non ledere possit idem.
Set qui frena tenet, prouiso tempore, presul,
1160 Quo magis est sanum ducet honestus iter:
Sepius ex dulci peccans sermone reviuit,
Qui magis impaciens verba per acra foret.
Expedit interdum tamen absque fauore rebelles
Equa quod inuitos presulis ira premat:
Sepe ferus morbus herbis mitescit amaris,
Namque feret molles aspera spina rosas:
Dura vides quod humus stimulantibus obruta
sulcis
Sepius ad placitum molle cacumen habet.
 Vnctus erit presul oleo, quod plura figurat,
1170 Precipueque sibi conuenit illa sequi:
Quatuor ista facit, penetrat, lucet, cibat, vngit,
Que sibi mitratus debet habere bonus.
Nil penetrare potest nisi cum virtute vigoris,
Ista tamen virtus in penetrando iuuat;
Nam cum mollicies fuerit coniuncta vigori,
Mitis et austerus presulis actus erit.
Fermento careat, oleo spergatur, vt absit
Culpa nocens, et eum sanctus inungat amor:
Vox ita doctoris, quanto sublimius intus
1180 Corda ferit, tanto forcius illa mouet:
Sic olei virtus virtutes ponderat eque,
Forcia dum penetrat, micius acta regit.
Lux cecis, cibus est ieiunis, vnccio morbis;
Hiis iubar infundit, hos cibat, hosque fouet:
Lux est exemplo, cibus est dum pascit egenos,
Vnccio dum populis dulcia verba serit.
Hoc oleo, testante Dauid, Cristus fuit vnctus,
Vnguine leticie cum pater vnxit eum:
Non vnguntur eo qui culpam Simonis equant,
1190 Qui vendunt vel emunt, nec sacra gratis habent:
Exulat hic de plebe dei qui peccat in istis,
Tales nam pellit Cristus ab ede sua.

Hic loquitur qualiter signa Anticristi in Curia

Romana precipue ex auaricia secundum
quosdam apparuerunt.

Capm. xiiii. Plura locutura mens deficit ipsa timore,

Labitur exanguis et tremefacta silet;
Huius enim vicii michi tangere si licet vlla,
Testis erit Cristus Romaque tota simul.
Roma manus rodit non dantes, spernit et odit,
Donum pro dono sic capit omnis homo.
Non est acceptor personarum deus, immo
1200 Gracior intendit actibus ipse viri;
Gracia set nostra tantum quos mundus in auro
Ditat, non alios accipit illa viros.
Qui precium ponit diues preciata reportat
Munera, nam tali curia tota fauet:
Assumens oleum secum non intrat ibidem,
Aurea ni valeat vngere gutta manum:
Copia nil morum confert vbi deficit aurum,
Nam virtus inopum nulla meretur opem.
Auro si pulses, intrabis, et illud habebis
1210 Quod petis, et donum fert tibi dona tuum:
Si tibi vis detur large, da munera larga,
Nam si pauca seras, premia pauca metes.
Quid faciet sapiens? stultus de munere gaudet.
Dicat ad hec clerus, qui sapit ista magis.395
Munera, crede michi, capiunt hominesque
deosque,
Placatur donis maior in orbe datis:
Set cum pro mundo tribuat sua munera Simon,
Promotus Cristi non erit inde sui.
Vt veniant ad aquas sicientes sponte citauit
1220 Cristus, et ecce suo fonte cibauit eos;
Fontibus et nostris siciens non hauriet vllus
Absque lucri pretio, quod dabit ipse prius.
Vendere quid pro quo modus est quem curia
nostra
Seruat, et auxilio Simonis ipsa viget:
Curia nostra virum nouit sine munere nullum,
Set redit in vacuis euacuata manus.
Dum dare vult laicus, precellit Theologiam;
Si des dona michi, dona rependo tibi:
Marcus, Matheus, Lucas, si nulla, Iohannes,
1230 Dona ferant, perdunt que sibi dona petunt:
Si veniat famulus mundi, viget ipse receptus,
Si famulus Cristi, nemo ministrat ei.
Si veniat pauper, musis comitatus Homeri,
Et nichil attulerit, pauper vt ante redit:
 Si nouus Augustinus ibi peteret, nec haberet
Quod daret ipse prius, transiet ipse vagus.
Construit atque legit laicus, bene cantat, in auro;
Si dare sufficiat, stat bene quicquid agit.
Qualis enim pietas hec est discernite vosmet,
1240 Aut si iusticia iura tenebit ita.
Si labat ecclesia declinans forte per istos,
Summus eam releuet de pietate sua,
Confundens hereses et que sunt scismata tollat,
Ne quis Cristicolas perdere possit oues:
Vnanimes redeant tibi, te miserante, redemptor,
Quos pax, quos pietas, quos liget vna fides.
Anticristus aget que sunt contraria Cristo,
Mores subuertens et viciosa fouens:
Nescio si forte mundo iam venerat iste,
1250 Eius enim video plurima signa modo.
Petri que titubat nauem prius erige, Criste,
Quam pereat, nec eam fastus in orbe voret.

Hic loquitur secundum commune dictum,

qualiter honores et non onera prelacie plures
affectant, quo magis in ecclesia cessant
virtutes, et vicia multipliciter accrescunt.

O deus, omne patet tibi cor loquiturque voluntas,

Capm. xv.
Et secreta tuo lumine nulla latent:
Tu nosti, domine, quod quantum distat ab ortu
Solis in occasum regula prima fugit.
Ipsa fides operans, quam tu plantare volebas
Est quasi de clero preuaricata modo:
Ius quod erat Cristi mundus sine iure resoluit,
1260 Prelatosque nouos vendicat ipse suos.
Nomen enim sancti sanctum non efficit, immo
Efficitur sanctus quem probat ipse deus.
Nos tamen a plebe si nomine glorificemur,
Et laudet mundus, laus placet illa satis:
 Laruata facie sic fallitur ordo paternus,396
Quo furtiuus honor expoliauit onus.
Vox populi cum voce dei concordat, vt ipsa
In rebus dubiis sit metuenda magis:
Hec ego que dicam dictum commune docebat,
1270 Nec mea verba sibi quid nouitatis habent.
In cathedram Moysi nunc ascendunt Pharisei,
Et scribe scribunt dogma, nec illud agunt.
Nam constans, humilis, largus, castus que
modestus,
Fit quibus ecclesiis regula culta prius,
Nunc vanos, cupidos, elatos, luxuriosos,
Raptoresque suo substituere loco.
Pacificos ira mitesque superbia vicit,
Nummus habet iustos et Venus illa sacros.
Sic non iusticia causas regit, immo voluntas
1280 Obfuscata malis que racione carent:
Sic modo terra deos colit et laceratur ab ipsis,
Est dum lex cleri nescia lege dei.
Nudis iam verbis vani tua iura figurant,
Et nichil aut modicum pondere iuris agunt;
Exemplis operum te raro, Criste, sequntur,
Perfectumque tue legis inane tenent.
Que tua precepta ponunt, deponere curant
A propriis humeris, que michi ferre iubent;
Hec precepta tamen que gloria ponit inanis,
1290 A me tollentes propria ferre volunt.
De fundamento non curant, immo columpne
Effigiem laruant, se quoque templa vocant.
Nuper erat celum corruptum, sicque superbus
Corruit ex altis, lapsus et yma tenet;
Proque suo vicio sic Adam de paradiso,
Sic Iudasque suum perdidit ipse gradum:
Non faciunt hominem status aut locus esse
beatum,
Quin magis hos sternunt qui superesse volunt.
O deus, ecclesiam fecisti quam tibi sanctam,
 1300 Sanctos prelatos fac simul inde tuos:
Corrigat, oro, deus, tua iam clemencia tales,
Nos quibus vt sanctis subdere colla iubes:
Esse duces nostros quos lege tua statuisti,
Fac magis vt recta semita ducat eos;
Et licet instabilis vanus sit et actus eorum,
Da populo stabilem semper habere fidem:
Da, deus, et clero, verbo quod possit et actu
Sic reuocare malum, nos vt in orbe iuuet.
Exoptata diu dulcis medicina dolorum,
1310 Sero licet veniat, grata venire solet:
Sique boni fiant de clero, nos meliores
Tunc erimus, que dei laus ita maior erit.

Postquam dictum est de illis qui errant in

statu prelacie, dicendum est de errore
curatorum, qui sub prelatis constituti,
parochiarum curas in animarum suarum
periculo admittentes negligenter omittunt: et
primo intendit dicere de curatis illis qui suas
curas omittentes ad seruiendum magnatum
curiis adherent.

Presulis incauti, sicut de voce recepi,

Capm. xvi.
Errores scripsi, pennaque cessat ibi.
Sunt tamen, in curis anime qui iura ministrant,
Rectores alii non sine labe doli.
Quo status ille modo se tendit scribere tendo,
Si sit ibi mundus vel magis ipse deus.
Ad tempus presens rectorum facta reuoluens,
1320 Inuenio mundi quod solet esse dei.
Presulis errore, curarum qui caput extat,
Errat curatus, presulis ipse manus.
Iam sine prebenda de Simonis arte creata
Nil putat ecclesiam quomodocumque bonam:
Hec prebenda tamen inopem non, set meretricem
 Pascit, sicque deum non colit, immo deam.
Tales nec caste curant neque viuere caute,
De quibus exempla sunt modo sepe mala:
Vestis habet pompam, cibus vsum deliciarum,
1330 Et thorus incestum clamat habere suum.
Ex Cristi poteris nuper cognoscere verbis
Discipulos tunicas non habuisse duas;
Set quia discipuli non sunt, in talibus isti
Nolunt impositum sic retinere modum.
Non tantum vestes geminant set condiciones,
Quas magis errantes regula nulla sapit:
En venit incastos aurum precingere lumbos,
Denotet vt vanos comptus inanis eos;
Militis effigie, nisi solum calcar abesse,
1340 Cernimus hos pompis degenerare suis.
Cuius honor, sit onus; qui lucris participare
Vult, sic de dampnis participaret eis:
Sic iubet equa fides, sic lex decreuit ad omnes,
Set modo qui curant ipsa statuta negant.
Curas admittunt pingues et pinguia sumunt,
Set nolunt cure pondera ferre sue.
Si viciis residere nequit curatus in ista
Cura, tunc aliam querit habere nouam;
Inficiens primam, post polluit ipse secundam,
1350 Sic loca non vicia mutat et ipse sua.
Litera dispensat curato presulis empta,
Et sic curati cura relicta manet;
Presbiterum laicum retinet sibi substituendum,
Curia magnatum dum retinebit eum.
Est vt apes ibi sollicitus dum spirat honores,
Set piger in cura tardat agenda sua.
Quicquid habet mundus fictum, tunc fingit et ille,
Curia quo dignum credere possit eum:
Verba dabit blanda, set nec canis aptus ad arcum,
1360 Sic humili vultu flectit ad yma genu.
Alter vt ille Iacob socios supplantat, et omne
Quo poterit mundi lucra tenere facit.
 Absit eum quicquam tamen absque iuuamine docti
Simonis incipere, qui suus actor erit:
Ostia si clausa fuerint, sic intrat ouile,
Ac aliunde suum carpit auarus iter.
De curis anime nil curat, dummodo terre
Curia magnatis sit sibi culta lucris:
Fert sibi nil virtus anime set corporis actus,
1370 Munus non meritum dat sibi ferre statum.
Qui nichil est per se, nec habet quo tendat in
altum,
Expedit alterius vt releuetur ope:
Est tamen absurdum, cum quilibet ex alieno
Intumet vlterius quam tumuisse decet.397
Littera dum Regis Papales supplicat aures,
Simon et est medius, vngat vt ipse manus,398
En l a i c u s noster fit clericus aptus vt omnes
Simone consultus scandat in orbe gradus.
Hic qui pauper heri fuerat quasi nudus et omni
1380 Laude carens, nec eum patris habebat honor,
Cuius erat tunica vilis, non larga set arta,
Vix sibi que tetigit simplicitate genu,
Hunc polimita modo vestis circumdat, et eius
Alludens pedibus fimbria lambit humum:
Vestis que medium non nouit poplicis olim,
Iam colit hec talos oscula dando pedi.
Si mundi speculum scruteris in huius amictu,
Plurima rectoris cernere vana potes.
Presulis ipse gradum si non dum scandere
possit,
1390 Ecce tamen vestes comparat ipse pares.
Cuius erat solus nuper catulus domicellus,
En sequitur totus nunc quasi mundus eum;
Cuius erat baculus nuper palfridus, ad eius
Sellam cum loris subditur altus equs.
Sic viget in curis diues, set moribus expers
Indiget, et vano more gubernat opes.
Compotus in mundi rebus quod fiat habunde
 Perstudet, vt domino det sua iura suo:
Computet vt Cristo set de curis animarum,
1400 Turpiter absque lucro fossa talenta latent.
Curia sic Cristi tollit mundana clientem,
Qui venit ad laqueum, dum sitit ipse lucrum.

Hic loquitur de rectoribus illis, qui ab

episcopo licenciati se fingunt ire scolas, vt sub
nomine virtutis vicia corporalia frequentent.

Alter adest rector, causam designat et ipse,

Capm. xvii.
Dicit enim sacras quod cupit ire scolas:
Vt vagus astet ibi prece ruffi presul et albi
Annuit, vt dominis quos a m a t i p s e n i m i s.
Sic rector sibi sub specie virtutis adoptat,
Vt queat in viciis rite studere vagus.
Nil decreta placent sibi nec sacra theologia,
1410 Ars sibi nature sufficit immo sue:
Ipsa magistra docet res plures, discit et ille,
Scribit et in nocte que studet ipse die.
Et propter formam tandem petit ipse cathedram,
Vt sit ad hoc ductus, plura dat ipse prius:
Sic est curatus doctoris sede locatus,
Datque legenda suis mistica iura scolis.
‘Ve soli,’ legimus ex scripturis Salomonis,
Namque virum solum nemo requirit eum:
Qua racione scole mos est, quod quisque studere
1420 Debet cum socia doctus in arte sua.
Ipse deus sociam fecit per secula primam,
Vt iuuet hec hominem, sicque creauit eam:
Masculus in primo factus fuit, atque secundo
Femina, sic vt in hiis det deus esse genus:
Istaque principia discretus rector agenda
Perstudet, et vota prebet in arte pia.
Quis laterisque sui costam quam sentit abesse
Non cuperet, per quam perficeretur homo?
 Prima viri costa mulier fuit ipsa creata,
1430 Vult igitur costam rector habere suam.
Nam deus humanam precepit crescere gentem,
Cuius precepto multiplicabit homo:
Sic sibi multiplicat rector, dum semen habundat,
Vt sit mandati non reus ipse dei.
Causas per tales rector probat et raciones,
Quod sibi sint socie, dum stat in arte scole.
Primo materiam conceptus tractat, et illam,
Vt veniat partus, stat repetendo magis;
Sic legit et textum, legit et glosam super illum,
1440 Vt scola discipulis sit patefacta suis:
Verberat ipse regens pro forma sepe scolares,
Vt vigili virga sit vigil ipsa scola.
Quanto formalis magis extat in arte legendo,
Est opus in tanto materiale minus.
Non labor excusat, doceat quin nocte dieque,
Quo sibi dat vacuum sollicitudo caput:
Questio namque sua, quam disputat esse
profundam,
Sentit et in casu plura profunda mouet.
Responsalis ei respondet ad omnia, quare,
1450 Nec sinit a logica quicquid abire sua;
Sepeque doctori concluditur, ipseque tantum
Confusus cathedra linquit inesse sua.
Leccio lecta nocet, decies repetita nocebit;
Dum legit inde magis, p l u s s i b i s e n s u s
h e b e s e s t.399
Et sic ars nostrum curatum reddit inertem,
De longo studio fert nichil ipse domum:
Stultus ibi venit, set stulcior inde redibit,
Dum repetendo scolis sit magis ipse frequens.
Hec est illa scola, studet in qua clerus, vt yma
1460 Nature iura scribat in arte sua:
Practica discipulo bene conuenit atque magistro,
Vt speculatiuum construat ipse suum.
Hec est illa scola super omnes labe colenda,
 Qua socius sociam g a u d e t habere suam:
Attamen illa scola, dum sit socie sociata,
Fine dabit socium plangere gesta reum.
Sic scola cum socia confirmat in arte scolarem,
Fiet quod laicus, quando magister erit.
Heu! grauis est socia, grauis est scola iuncta
sodali,
1470 Ista vorat corpus, illaque tollit opes:
Est inhonesta deo res, et mirabile plebi,
Quando magister erit atque ribaldus idem.
Ecclesia sponsa nuda, vestitur amica;
Sponsa relicta perit, altera cara viget:
Sic desponsata clamare fide sibi fracta
Nunc venit ecclesia iura petendo sua.
Set quia lux periit, perit hinc ius, sicque recedit
Curati sponsa stans quasi tota vaga.
Sic rector viciis studium non moribus aptans,
1480 Dat decimam Veneri, que solet esse dei:
Sic sibi consimilem generat curatus, vt artem
Nature solitam compleat ipse suam:
Sic viget in studio laici curatus ad instar
Corporis, vt sexum multiplicare queat:
Sic scola, que morum mater magis esse solebat,
Efficitur viciis stulta nouerca suis.

Hic loquitur de rectoribus illis, qui in curis

residentes, curas tamen negligentes,
venacionibus precipue et voluptatibus penitus
intendunt.
Capm. xviii. Tercius est rector, animum qui tendit ad orbem,
In cura residens dum manet ipse domi:
Nuda sue folia cure sine fructibus affert,
1490 Dum sine luce regens stultus obumbrat eam.
Predicat ipse nichil animas saluare, nec egros
Visitat, aut inopes tactus amore iuuat:
Est sibi crassus equs, restatque sciencia macra,
Sella decora que mens feda perornat eum.
Ad latus et cornu sufflans gerit, vnde redundant
Mons, nemus, vnde lepus visa pericla fugit;
Oris in ecclesia set vox sua muta quiescit,
Ne fugat a viciis sordida corda gregis.400
Sic canis, ad questum qui clamat in ore fideli,
1500 Certus habebit eo quicquid habere velit;
Set miser, ad portas qui clamat et indiget escis,
Heu! neque mica datur nec liquor vllus ei.
O deus, in quanta talis tibi laude meretur,
Dans alimenta cani, que negat ipse viro!
Vix sibi festa dies sacra vel ieiunia tollunt,
Quin nemus in canibus circuit ipse suis:
Clamor in ore canum, dum vociferantur in vnum,
Est sibi campana, psallitur vnde deo.
Stat sibi missa breuis, deuocio longaque campis,
1510 Quo sibi cantores deputat esse canes:
Sic lepus et vulpis sunt quos magis ipse requirit;
Dum sonat ore deum, stat sibi mente lepus.
Sic agitat vulpis vulpem similis similemque
Querit, dum iuuenem deuorat ipse gregem;
Nam vagus explorat vbi sunt pulchre mulieres
Etatis tenere, pascat vt inde famem:
Talis enim rector mulieribus insidiatur,
More lupi clausas circuientis oues.401
Dum videt ipse senem sponsum sponsam
iuuenemque,
1520 Tales sub cura visitat ipse sua;
Suplet ibi rector regimen sponsi, que decore
 Persoluit sponse debita iura sue.
Sic capit in cura rector sibi corpora pulcra,
Et fedas animas linquit abire vagas.

Hic loquitur de rectoribus in curis

residentibus, qui tamen curas animarum
omittentes, quasi seculi mercatores singula
temporalia de die in diem ementes et
vendentes, mundi diuicias adquirunt.

Quartus adhuc rector curam residendo sinistrat,

Capm. xix.
Ipseque mercator circuit omne genus.
Est sibi missa: forum meditatur et inde tabernam,
Ad socii dampnum dum petit ipse lucrum.
Ecclesie meritum perdit, lucratur et aurum;
1530 Vt teneat mundum, deserit ipse deum.
Computat ipse diem cassam, qua vel sibi lucrum,
Corporis aut luxum non capit ipse nouum:
Est et auaricia sibi custos, sic vt in illis402
Partem diuiciis pauper habere nequit.
Masculus in nullo casu partitur egenus,
Dupplice nam claui cista resistit ei;
Set pietas aliter se continet ad mulierem,
Vt iubet ipsa Venus, est ibi larga manus.
Expansis genibus expanditur aurea cista;
1540 Femina si veniat, dat sibi clauis iter:
Durior est ferro, quem nullus mollificabit,403
Vincit feminea set caro mollis eum.
Dans ita quid pro quo merces mercede locabit
Rector, in impropriis dum vacat ipse lucris.
Omne quod vna manus sibi congregat, altera
spergit,
Dum sua dat cribro balsama stultus homo:
Stultaque sic stultum predat, quod fine dierum
Nil nisi sit rasa barba manebit ei.
O si curatis nati succedere possent,
 1550 Ecclesie titulo ferreque iura patrum,
Tunc sibi Romipetas, mortis quibus est aliene
Spes, nichil aut modicum posse valere puto.404
Talis in ecclesia nunc est deuocio mota
Curatis nostris: iudicet inde deus.

Postquam405 dictum est de errore illorum

qui in ecclesia beneficiati existunt, iam406
dicendum est de presbiteris stipendiariis; de
talibus saltem, qui non propter mundiciam et
ordinis honestatem, set propter mundi ocia,
gradum presbiteratus appetunt et assumunt.

Si de presbiteris dicam qui sunt sine curis,

Capm. xx.
Hos viciis aliis cernimus esse pares.
Si tamen ecclesiam non optinet iste sacerdos,
Annua servicia sunt velut ecclesia:
Plus quam tres dudum nunc exigit vnus
habendum,
1560 Strictus auaricia plus cupit ipse quia.
Hos velut artifices cerno peditare per vrbes,
Conductos precio sicut asella foro.
Dignus mercede tamen est operarius omnis,
Iuxta condignum quod labor ille petit:
Set tamen vt vendat nulli diuina licebit,
Sic poterit vendi missa nec vlla tibi.
Credimus vt sancta Cristus sacratus in ara
Non plus vult vendi venditus ipse semel.
Se sine dat precio, dare qui iussit sacra gratis:
1570 Presbiter, ergo tibi quid petis inde lucri?
Cum tibi vestitus, aptus fuerit quoque victus,
Vnde deo viuas, cur tibi plura petis?
Si tibi plus superest de lucro, nil tibi prodest,
Nam male quesitum nescit habere modum.
Aut Romam perges mercatum Simonis auro,
Qui te promotum reddet, et inde tuum
 Argentum tollet collectum per prius, et sic
Quod tibi missa dedit Simon habere petit;
Aut meretrix bursam, te luxuriante, repletam
1580 Sugget, et in vacuam quam cito reddet eam.
Quod dedit ecclesia tollit meretrix que taberna:
Hec tria dum iungunt, turpia plura gerunt.
Hec ita cum videam, mundi noua monstra
putarem,
Si foret hoc raro quod speculamur eo;
Set quia cotidie potero predicta videre,
Sepe michi visa nil modo miror ea.
Mergulus inmergit fluuio sua membra frequenter,
Et longas gignit in latitando moras;
Isteque signat eos quos carnis fluxa voluptas
1590 Funditus exercet et retinendo premit.
Est apud antiquos ‘hic et hec’ dixisse ‘sacerdos,’
Dicere sic et nos possumus ‘has et eos:’
Hii modo namque sua mundum replent genitura;
Si pietas sit ibi, sunt modo valde pii.
Nox et amor, vinum, nullum moderabile suadent,
Que tria presbiteris sunt modo nota satis.
Stat breuis ordo precum, dum postulat ipse
vicissim
Oscula per longas iungere pressa moras,
‘O sacer,’ hec dicens, ‘quam longum tempus ad
illud
1600 Vt tua sint collo brachia nexa meo?’
Qui vult vxorem seruare sibi modo castam,
Et mundas cameras querit habere suas,
Longius a camera sit presbiter atque columba,
Stercora fundit ea, fundit et ipse stupra.
Sobrius a mensa, de lecto siue pudicus
Consurgit raro presbiter ipse deo:
Cantat in excelsis sua vox agitata tabernis,
Est set in ecclesiis vox tac i t u r n a nimis:
Doctus et a vino colit ipse lupanar, et illuc
1610 Exorando diu flectit vtrumque genu.
 Sic vetus expurgat fermentum, dum noua spergit,
Non tamen vt Paulus iusserat ipse prius:
Sic altare Baal modo thurificare sacerdos
Vult, per quem viui feda fit ara dei.
Sufficit vna michi mulier, bis sex tamen ipsi,
Vt iuueni gallo, cerno subire modo.
Sic sacra presbiteri celebrant solempnia Bachi,407
Ebrietasque magis sanctificatur eis.
Gentilis ritus vetus incipit esse modernus,
1620 Talibus et Cristi lex perit ipsa quasi:
Sic modo templorum cultores suntque deorum,
Plus in honore quibus stat dea summa Venus.

Hic loquitur de consueta presbiterorum

voluptate, et qualiter hii stipendia plebis ex
conuencione sumentes, indeuote pro mortuis
orando non se debite ad suffragia mortuorum
exonerant.

Ignis edax terram vorat et nascencia terre;

Capm. xxi.
Quo furit illius impetus, omne terit;
Sic et in incastis exemplis presbiterorum
Indoctis laicis feda libido nocet.
Nil commune gerunt luxus sibi cum racione,
Corporeos sensus quinque libido cremat:
Quos talis maculat nota talis pena sequetur,
1630 Illorum pene sulphur et ignis erunt.
Consuetudo tamen solet attenuare pudorem,
Reddit et audacem quem mora longa trahit.
Non peccare putant quod sepius oscula iungant,
Oscula nam pacis signa parare solent;
Estque parare piam pacem meritoria causa,
Nec sine pace diu stat pietatis amor:
Sic in presbiteris amor est de pace creatus,
Oscula nam solito more frequenter agunt.
Altera natura solitus reputabitur vsus,