Alanine Aminotransferase

(ALT)
Antonio, Patrica
Aquino, Zenyll
Baravio, Keenjo
 Liver Enzymes

Alanine Aminotransferase

Also known as SGPT or serum glutamic pyruvic transaminase

Transfer amino group from alanine to alpha ketoglutarate with
formation of glutamate and pyruvate
Major source: Liver (hepatocytes)
Other source: Kidney, Pancreas, RBC, Heart, Skeletal muscles, Lungs
Significance: Hepatocellular disorders and acute inflammatory
conditions of liver
 Alanine Aminotransferase

COENZYME
Clinical Significance
Pyridoxal phosphate -
Increase with liver disease. activates ALT
It also monitors the course of hepatitis treatment and the No pyridoxal phosphate
effects of drug therapy
no ALT function
ALT measurement is a more sensitive and specific screening
test for posttransfusion hepatitis or occupational toxic
exposure
Not affected by hemolysis
The clinical situation in toxic hepatitis is
different from that infectious hepatitis
ALT Determination
Walker Method

Reitman-Frankel Method

De Ritis Ratio
 ALT Determination
Walker Method REITMAN-FRANKLE DE RITIS RATIO
Coupled Enzymatic Reaction Colorimetric Method The AST/ALT ratio
Pyruvate from ALT activity For the determination of Differentiates the cause of
reacts with LDH as the the Hepatic disorder
indicator enzyme
GPT concentration in
Ratio > 1 - Non viral origin
Uses LD and monitors human serum.
(Alcohol Hepatitis: Acute
decrease in absorbance at Reagent: 2,4 dinitrophenyl Hepatitis)
340 nm. hydrazine (2,4 -DNPH) Ration < 1 - Viral in origin
Reference range: 6-37 u/L End Color: Brown
-Darker: Means increased
enzyme activity
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