TOPIC: SUBCUTANEOUS FUNGI BATCH 2024

SUBCUTANEOUS FUNGI PHIALOPHORA VERRUCOSA

SPOROTHRIX SCHENCKII
MICROSCOPIC EXAMINATION
 Delicate branching hyaline, septate hyphae 1
to 2 um wide with conidiophores that
terminate in clusters of small pyriform
conidia producting a “flowerette
arrangement”
 Conidiophores arise at right angles from thin
septate hyphae and are usually solitary erect
and tapered toward the apex
MICROSCOPIC EXAMINATION
 Dark, funnel-shaped collarettes at the tips of
the conidiogenous cells (phialides) in
Phialophora verrucosa. Also note the oval-
shaped conidia
 Vase-shaped phialides with darkly stained,
cup-shaped collarettes, topped with cluster
of smooth-walled conidia

“FLOWERETTE ARRANGEMENT”
CULTURE
 Culture of Sporothrix schenckii (Sabouraud
media, 28 °C)
 Moist and glabrous, with a wrinkled and
folded surface
CULTURE
 Some strains may produce aerial hyphae and
 Colonies are woolly to velvety, dark grey,
pigmentation may vary from white to cream
brown or olivaceous black on the surface and
to black
reverse.

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 TOPIC: SUBCUTANEOUS FUNGI BATCH 2024

FONSECA COMPACTA FONSECA PEDROSOI

MICROSCOPIC EXAMINATION MICROSCOPIC EXAMINATION
 Septate, dark brown hyphae and suberect •Rhinocladiella type
conidiophores that highly branch at apices of conidiation
 The conidiophores are pale, brown, erect, •Conidiophores are
septate, and sympodial with conidiogenous septate, erect and
zones confined to the upper portion. sympodial; swollen
denticles bear ovoid
Complex conidial head: conidia at the tip and
 1- celled conidia arise from swollen denticles along the side of the
located at tips of conidiophores (Primary conidiophore.
Conidia). •Usually only primary
 Primary Conidia function as sympodial conidia develop
conidiogenous cells, becoming irregularly
swollen at their apices  give rise to •Sympodial
secondary conidia on swollen denticles growth of the
 Secondary conidia produce tertiary series of hyphae/
conidia like those formed by the primary conidiophore
conidia. (arrow)

• Conidia have
dispersed
revealing the
sympodial growth
pattern (arrows)

CULTURE
a.) LPCB- stained microculture showing septate
hyphae with erect conidiophores and ovoid
conidia located either at the end or at the
side of conidio-phores, characteristic of the
genus Fonsecaea. (X 400 Objective)
b.) Dark, Olivaceous filamentous colonies in
Sabouraud Dextrose Agar cultured for 10
days, at 25°C
CULTURE
Fonsecaea pedrosoi- SDA, -3 weeks, 30°C
 Colony surface may be dark green to olive
brown to dark grey or jet black depending on
the strain and medium; covered with a fine,
velvety or downy mycelium
 Usually produce a raised convex protrusion at
the center where initially inoculated
 The colony reverse is black

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 TOPIC: SUBCUTANEOUS FUNGI BATCH 2024

CLADOPHIALOPHORA CARIONII/ CLADIOPHORA

CARIONII
MICROSCOPIC EXAMINATION
 Ascending to erect, olivaceous-green, apically
branched, elongate conidiophores producing
branched acropetal chains of conidia
 Chains of conidia, emanating from septate
conidiophores, which in turn, branched from
the organism’s septate hyphae
 Conidia are pale olivaceous, smooth-walled or
slightly verrucose, limoniform to fusiform, Colony of Cladiophora Carionii
1.5-3.0 x 2.0-7.0 um in size. MADURELLA SPP.
MICROSCOPIC EXAMINATION
 Wet mount from culture stained with
Lactophenol Cotton Blue (LPCB) showing
brown pigmented hyphae with intercalary
chlamydospores (arrow) characteristic of
Madurella mycetomatis

 Cladophialophora species showing branched

chains of oval conidia extending from main
hyphae
 Only a few free conidia are present (inset)
and these show a hilus(H) where the conidia
once were attached.
 Although most cultures are sterile, two types
of conidiation have been observed:
1. The first being flask-shaped phialides that
bear rounded conidia.
2. The second being simple or branched
conidiophores bearing pyriform conidia ( 3-5
um) with truncated bases.

CULTURE

 Colonies are slow growing, reaching 3-4 cm in

diameter after one month, with a compact
suede-like to downy surface and are
olivaceous-black in color.

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 TOPIC: SUBCUTANEOUS FUNGI BATCH 2024

CULTURE
 Colonies are slow growing, flat and leathery
at first, white to yellow to yellowish-brown,
becoming brownish, folded and heaped with
age, and with the formation of aerial mycelia.
 A brown diffusible pigment is
characteristically produced in primary
cultures

Transcribed by: LugawMD Page 4