ASSIGNMENT

To four 10.0 mL aliquots of water sample were added 1.50, 3.00 and 4.50 mL of
standard NaF solution containing 15.0 ppb. Exactly 3.00 mL of solution containing an
excess of Al-acid Alizarin Garnet R complex, a strongly fluorescent complex, were
added to each and solutions were diluted to 50.00 mL. The fluorescent intensity of the
four solutions plus a blank were as

mL Sample mL of Standard F‒ Meter Reading

10.00 0.00 65
10.00 1.50 52
10.00 3.00 39
10.00 4.50 27

(i) Explain the chemistry of the analytical method.

(ii) Plot the data.
(iii) By least squares compute the regression parameters of the linear equation
relating the decrease in fluorescence to the volume of standard reagent.
(iv) Calculate the standard deviation of the slope.
(v) Calculate the concentration of F‒ in ppb in the sample.