Introduction for formaldehyde and formal

saline Ethanol
Formaldehyde and formal saline ethanol are
commonly used solutions in laboratory settings
for tissue fixation and preservation.
Formaldehyde, also known as formalin when
dissolved in water, is a widely used fixative in
histology and pathology laboratories for
preserving tissue samples for microscopic
examination. Formal saline ethanol is a
modified solution containing formaldehyde,
saline, and ethanol, used for tissue fixation and
dehydration in histological processing. These
solutions play a crucial role in maintaining the
structural integrity of tissues and cells,
allowing for accurate histological analysis and
research. Understanding the properties and
applications of formaldehyde and formal saline
ethanol is essential for researchers,
histotechnologists, and pathologists working
with tissue specimens in laboratory settings.
Equipment & Glassware

—Graduated Cylinder (10mL)

—Graduated Cylinder (100mL)

—Wash Bottle

—Latex gloves

Chemical & Reagent

—formaldehyde

—ethanol %30

—water
Discussion
When discussing the use of formaldehyde and formal
saline ethanol in laboratory settings, it is important to
consider the following points:

1. Formaldehyde:

- Properties: Formaldehyde is a colorless, pungent gas

that is commonly used in the form of formalin, a solution
of formaldehyde gas dissolved in water.

- Applications: Formaldehyde is a widely used fixative in

histology and pathology laboratories for preserving
tissue samples for microscopic examination.

- Fixation: It works by cross-linking proteins in the

tissue, stabilizing cellular structures, and preventing
degradation.

- Concentration: The typical concentration of

formaldehyde in formalin solutions ranges from 10% to
4% depending on the application.

2. Formal Saline Ethanol:

- Composition: Formal saline ethanol is a modified
solution containing formaldehyde, saline (sodium
chloride solution), and ethanol.

- Applications: It is used for tissue fixation and

dehydration in histological processing, combining the
fixative properties of formaldehyde with the dehydrating
properties of ethanol.

- Dehydration: Ethanol in the solution helps remove

water from the tissue, aiding in the preservation process
and subsequent embedding in paraffin or resin.

3. Safety Considerations:

- Formaldehyde Exposure: Both formaldehyde and

formal saline ethanol solutions should be handled with
caution due to their potential health hazards.

- Safety Precautions: Proper ventilation, personal

protective equipment (PPE), and adherence to safety
guidelines are essential when working with these
solutions to minimize exposure risks.

4. Storage and Handling:

- Proper Storage: Formaldehyde and formal saline

ethanol solutions should be stored in tightly sealed
containers, away from heat and direct sunlight to prevent
degradation.

- Labeling: Clear labeling with concentration, date of

preparation, and hazard warnings is crucial for safe
handling and identification.

5. Regulatory Compliance:
- Regulations: Laboratories must adhere to regulatory
guidelines and safety standards regarding the use,
storage, and disposal of formaldehyde-containing
solutions.

- Waste Disposal: Proper disposal methods for

formaldehyde waste should be followed to minimize
environmental impact and ensure compliance with
regulations.

By considering these points, laboratory personnel can

effectively utilize formaldehyde and formal saline ethanol
solutions for tissue fixation and preservation while
prioritizing safety, quality, and regulatory compliance in
their work. Regular training, awareness of safety
protocols, and proper documentation are essential
aspects of working with these solutions in laboratory
settings.
Result

Ethanol 30% is commonly used in the

laboratory for various purposes such as
purification and precipitation of bio
molecules,staining and restraining
specimens in histology,dehydrating
tissues before embedding,and
disinfection
Ethanol is a common solvent in
laboratories as it mixes easily with water
and organic content.Ethanol is also used
as a fuel and renewable biofuel. In terms
of disinfection,70% denatured ethanol
mixed with 30% water is the most
effective concentration for killing
bacteria,fungi, and viruses
Reference

https://pubchem.ncbi.nlm.nih.gov/comp
ound/Ethanol

https://www.cancer.gov/about-
cancer/causes-
prevention/risk/substances/formaldehyd
e