α1-Ac GLY

α1-Acid Glycoprotein 4+1

Turbidimetry

PREPARATION AND STABILITY (BIREAGENT)

SPINLAB 180
Ref. 1102154. Both reagents are ready to use.
The reagents are stable until the expiration date stated on the label.

CALIBRATION

Use the general proteins calibrator (Ref. 1102003). Prepare the dilutions according with the instructions stated on the instruction
sheet included in the kit.

OPERATORY CONDITIONS

Introduce the following data in the computer :

Name Glycoprot. Ref. male low 50 mg/dL

Abbr. Name GLYC Ref. male high 120 mg/dL
Mode Twopoint Ref. female low 50 mg/dL
Wavelength 340 nm Ref. female high 120 mg/dL
Units mg/dL Ref. ped. low *
Decimals 0 Ref. ped. high *
Low Conc. 10 mg/dL Control 1 *
High Conc. 250 mg/dL Control 2 *
Calibrator name SP Calibrator Control 3 *
Prozone check No Factor de correl. 1.000
Correlat. offset 0.000

DUAL MODE MONO MODE

Sample Blank No Sample Blank No

R1 bottle 25 mL R1 bottle 25 mL
normal volume 240 μL normal volume μL
rerun volume 240 μL rerun volume μL
Sample Sample
normal volume 2.0 μL normal volume μL
rerun volume 2.0 μL rerun volume μL
R2 bottle 5 mL
normal volume 60 μL
rerun volume 60 μL
Predilution No Predilution No
Slope Blank No Slope Blank No
Point one, two 6, 236 sec. Point one, two .

Reagent blank No Reagent Blank No

Low absorbance -0.100 Abs Low absorbance -0.100 Abs
High absorbance 3.000 Abs High absorbance 3.000 Abs
R.Abs.L.Limit -0.100 Abs R.Abs.L.Limit -0.100 Abs
R.Abs.H.Limit 3.000 Abs R.Abs.H.Limit 3.000 Abs
Substr. Depletion 3.000 Abs Substr. Depletion 3.000 Abs

MEASUREMENT RANGE

The measurement interval for this method is 10 - 250 mg/dl of α1-Acid Glycoprotein.

ITAS34-I Ed. 01/ 2006 SPINREACT,S.A.U. Ctra.Santa Coloma, 7 E-17176 SANT ESTEVE DE BAS (GI) SPAIN
Tel. +34 972 69 08 00 Fax +34 972 69 00 99. e-mail: spinreact@spinreact.com
 α1-Ac GLY

α1-Acid Glycoprotein 4+1

Turbidimetry

PREPARATION AND STABILITY (MONOREAGENT)

SPINLAB 180
Ref. 1102154. Both reagents are ready to use.
The reagents are stable until the expiration date stated on the label. Reagents R1 and R2 are mixed with 4 parts R1 plus 1 part
R2.

CALIBRATION

Use the general proteins calibrator (Ref. 1102003). Prepare the dilutions according with the instructions stated on the instruction
sheet included in the kit.

OPERATORY CONDITIONS

Introduce the following data in the computer :

Name Glycoprot. Ref. male low 50 mg/dL

Abbr. Name GLYC Ref. male high 120 mg/dL
Mode Endpoint Ref. female low 50 mg/dL
Wavelength 340 nm Ref. female high 120 mg/dL
Units mg/dL Ref. ped. low *
Decimals 0 Ref. ped. high *
Low Conc. 10 mg/dL Control 1 *
High Conc. 250 mg/dL Control 2 *
Calibrator name SP Calibrator Control 3 *
Prozone check No Factor de correl. 1.000
Correlat. offset 0.000

DUAL MODE MONO MODE

Sample Blank No Sample Blank No

R1 bottle 25 mL R1 bottle 25 mL
normal volume 300 μL normal volume μL
rerun volume 300 μL rerun volume μL
Sample Sample
normal volume 2.0 μL normal volume μL
rerun volume 2.0 μL rerun volume μL
R2 bottle 5 mL
normal volume 0 μL
rerun volume 0 μL
Predilution No Predilution No
Slope Blank No Slope Blank No
Incubation 4.5 min. Point one, two .

Reagent blank Yes Reagent Blank No

Low absorbance -0.100 Abs Low absorbance -0.100 Abs
High absorbance 3.000 Abs High absorbance 3.000 Abs
R.Abs.L.Limit -0.100 Abs R.Abs.L.Limit -0.100 Abs
R.Abs.H.Limit 3.000 Abs R.Abs.H.Limit 3.000 Abs
Substr. Depletion 3.000 Abs Substr. Depletion 3.000 Abs

MEASUREMENT RANGE

The measurement interval for this method is 10 - 250 mg/dl of α1-Acid Glycoprotein.

ITAS34-I Ed. 01/ 2006 SPINREACT,S.A.U. Ctra.Santa Coloma, 7 E-17176 SANT ESTEVE DE BAS (GI) SPAIN
Tel. +34 972 69 08 00 Fax +34 972 69 00 99. e-mail: spinreact@spinreact.com