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(Download PDF) Microbiological Methods For Environment Food and Pharmaceutical Analysis Abhishek Chauhan Online Ebook All Chapter PDF
(Download PDF) Microbiological Methods For Environment Food and Pharmaceutical Analysis Abhishek Chauhan Online Ebook All Chapter PDF
(Download PDF) Microbiological Methods For Environment Food and Pharmaceutical Analysis Abhishek Chauhan Online Ebook All Chapter PDF
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Abhishek Chauhan
Tanu Jindal
Microbiological
Methods
for Environment,
Food and
Pharmaceutical
Analysis
Microbiological Methods for Environment, Food
and Pharmaceutical Analysis
Abhishek Chauhan • Tanu Jindal
© The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature
Switzerland AG 2020
This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of
the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation,
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The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication
does not imply, even in the absence of a specific statement, that such names are exempt from the relevant
protective laws and regulations and therefore free for general use.
The publisher, the authors, and the editors are safe to assume that the advice and information in this book
are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the
editors give a warranty, expressed or implied, with respect to the material contained herein or for any
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Preface
Discovery of microorganisms and the field microbiology began with the invention
of the first microscope in the year 1665, and since then, exponential growth of this
science can be seen. In fact, the current world largely depends upon the various
aspects of microbes, organisms that are invisible to the naked eye. These tiny organ-
isms demonstrate their presence in both positive and negative facets of life.
Microorganisms including bacteria, yeast, molds, etc., are ubiquitous in nature and
their applications are as well omnipresent throughout. Right from the air, soil, and
water, that is, three main constituents of the Earth, the applicability of microbes can
be found in any means. This science is gaining importance globally as far as the
safety and quality of various commodities such as food, water and wastewater, air,
cosmetics, drugs and pharmaceuticals, and antimicrobial preparation such as paints,
emulsions, herbal preparations, and medical devices are concerned. Safety and
quality evaluation of these commodities is a foremost requirement in order to pro-
vide the best services to mankind and for the betterment of society.
Microbes are significant to all our lives in enormous ways. Sometimes, the influ-
ence of microorganisms on human life is beneficial, whereas at other times, it is
detrimental. For example, microorganisms are required to produce cheese, yogurt,
bread, alcohol, antibiotics, vaccines, vitamins, enzymes wine, beer, etc. Several
microbial products contribute to public health such as aiding in nutrition, interrupt-
ing the spread of disease, and improving the quality of life in the years ahead.
Undesirable presence of microorganisms in various commodities such as food,
water, drugs, and pharmaceutical products makes it essential to ensure the safety
and quality of these products, which is usually achieved by quality evaluation or
analysis by carrying out microbiological experiments.
The textbook Microbiological Methods for Environment, Food and
Pharmaceutical Analysis specifically aims at the ever-demanding thoughtful need
of a well-documented compilation of factual details. The book emphasizes specifi-
cally on applied aspects of industrial microbiology including water, soil, air, food,
and pharmaceuticals. Good Microbiological Laboratory Practices (GMLP) cover-
ing all aspects of Good Laboratory Management System is well written in a techni-
cal manner so that if applied, it would aid in creating a state-of-the-art laboratory.
v
vi Preface
Special elaborations have been given to management with respect to safety, house-
keeping, spillage, disposal, and avoidance of laboratory-acquired infections.
Importance of microbiological culture media and their composition has also been
given so as to inform the user about ingredients of the media having characteristic
behavior during experiment. Knowledge of sterilization and disinfection procedure
from the point of view safety is an utmost requirement in laboratory while working
with highly contaminated sample and handling pathogenic culture. A concise reve-
lation about major and minor instruments/equipment used in industrial microbiol-
ogy has been given. Microbiological methods have been written in simple, lucid,
and crisp language keeping in view the requirement of industries and research orga-
nizations working on quality evaluation of variety of products. A fairly up-to-date
“Index” in the textbook will surely enlarge the vision of its readers by giving an easy
access of subject-enriched, well-documented text.
The book is highly useful for industrialists, researchers, academicians, and stu-
dents from both undergraduate and postgraduate levels of microbiology, biotechnol-
ogy, botany, and pharmaceutical sciences. It would have significant contribution in
the effective knowledge of scientists/analysts/lab personnel/technical managers/
quality managers working with the industrial aspect of microbiology. The authors
are grateful that Springer Nature has enthusiastically agreed to publish this work
and appreciates their help and collaboration on this project.
The current book has been intended with various microbiological methods pertain-
ing to water, soil, air, food, and pharmaceutical analysis with respect to quality and
safety evaluation of products. As per the requirements of different sectors, this text-
book has been divided into several chapters of current microbiological research
interest. Chapters 1 and 2 introduce a detailed knowledge of good microbiological
and management practices, Chap. 3 is designed to provide the essential information
pertaining to various types of microbiological culture media and their types and role
of ingredients. Chapter 4 describes various methods of sterilization and disinfec-
tion. Microbiological experiments required several major and minor equipment;
therefore, Chap. 5 has been designed in order to provide the necessary information
with respect to all required instrumentations and equipment. Chapter 6 describes
methods of sampling for water, soil, air, food, and pharmaceutical products. The
Chaps. 7, 8, and 9 demonstrate the standard laboratory procedures for carrying out
the analysis of water, soil, air, food, and pharmaceuticals. Methods written in this
book are significantly sub-headed to make it more user-friendly with better accu-
racy for evaluation of the products for betterment of mankind and society. Chapter 10
describes various methods relating to biochemical and molecular characteristics of
microorganisms in order to identify the microbe effectively. The script is also
equipped with relevant tables, figures, and photographs. The textbook specifically
aims at the ever-demanding thoughtful need of a well-documented compilation of
factual details. The book is written in simple, lucid, and crisp language keeping in
view the requirement of industries and research organizations working on quality
evaluation of a variety of products.
Abhishek Chauhan
Tanu Jindal
vii
Contents
ix
x Contents
Rev. Mr. Baller also had been associated with Dr. Mateer in the
revision of the Scriptures since 1900. He says of him:
Ada, who in the last eight years of his life stood nearer to him by
far than any other, and knew his innermost life, puts on record this
supplement as to some of his traits not so fully brought out by the
testimony of his friends:
The English Baptist Mission at their first meeting after his death
adopted resolutions expressive of their deep sense of loss. One of
these will serve as an example of all:
Combined with great strength of will and an enthusiasm
which overcame all difficulties and opposition which stood in
the way of the accomplishment of the great and arduous
tasks, he was endowed with much tenderness of heart and a
devoted loyalty to the gospel. He was a successful educator,
a fine administrator, a powerful preacher, and a distinguished
scholar; and his removal from amongst us has left a gap
which will not soon or easily be filled.
Parentage, 16, 18
Pastorate, 179, 180, 192
Pecuniary affairs, 171, 172
Peking, 264, 278, 285, 294
Periodicals and contributions, 150
Persecutions, 187, 195