V [S ] FORMULARIO PARCIAL 2 Lineweaver-Burk

V = max Enzimología y Biocatálisis 1 KM 1 1

K M + [S ] = ⋅ +
V Vmax [ S ] Vmax
Fracciones
Vmax = kcat [ET] V [S ] Eadie-Hofstee
fES = =
Vmax K M + [S ] ⎛V ⎞
V = − K M ⋅ ⎜⎜ ⎟⎟ + Vmax
[ EI ] = [I ] [ ES ] = [S ] ⎝ [S ] ⎠
[ ET ] Ki ⎛1+ [ S ] ⎞ + [ ET ] K ⎛ [I ] ⎞ + Hanes-Wolf
⎜ ⎟ [I ] M ⎜1+ ⎟ [S ]
⎝ KM ⎠ Ki ⎠
⎝
[S ] = 1
⋅ [S ] +
KM
V Vmax Vmax
Inhibiciones
1 KM ⎡ [I ] ⎤ 1 1 1 ⎛αKM ⎞ 1 ⎛ α ' ⎞
= ⋅ ⎢1 + ⎥ ⋅ + M =⎜ ⎟ +⎜ ⎟
C V Vmax ⎣ K I ⎦ [S ] Vmax V ⎝ Vmax ⎠ [ S ] ⎝ Vmax ⎠
" [ I] % [ ES ] I [E ] [I ]
Ki =
[ E][I ] ap
K M = K M $1+ ' K i' = [] Ki =
[ EI ] # K I & [ ESI ] [ EI ]
1 ⎛ KM 1 1 ⎞⎛ [I ] ⎞ 1 KM 1 1 ⎛ [I ] ⎞
NC = ⎜
⎜ ⋅ + ⎟⎟⎜⎜1 + ⎟⎟ AC = ⋅ + ⎜⎜1 + ⎟⎟
V ⎝ Vmax [S ] Vmax ⎠⎝ K I ⎠ V Vmax [S ] Vmax ⎝ K I ⎠
€ Vmax Vmax KM
Vmax ap =
[ I]
Vmax ap =
[ I] K M ap =
1+ 1+ [ I]
KI KI 1+
K
 FORMULARIO PARCIAL 2
Enzimología y Biocatálisis

A = εbc
ΔG ++
kB T −
0
ΔG' = ΔG '+RT lnK eq k= e RT
h
Ea
ln k = ln A − k BT ΔH + + ΔS + +
RT ln k = ln − +
h RT R
€
k2 Ea ⎛ 1 1 ⎞€
ln = ⎜ − ⎟ ⎛k T ⎞
k1 R ⎝ T1 T2 ⎠ ΔS ++ = R ln A − R ln ⎜ B ⎟ − R
⎝ h ⎠
ΔH ++ = Ea − RT

KB= 1.38×10−23 J/K h = 6.62x10-34 J/seg

J
k= PZe-Ea /RT R = 8.314
mol K