CROSS MATCH MAYOR MINOR AUTO CONTROL

PROCEDURE Cross Match Mayor

1. Buat Suspensi Sel Eritrosit Donor 1% (5 ul Sel Eritrosit + 500 ul Diluent LISS)
2. Pipet 50 ul Suspensi Sel Eritrosit Donor Masukkan Lewat Dinding Micro Tubes
3. Pipet 25 ul Serum/Plasma Pasien Masukkan Lewat Dinding Micro Tubes
4. Inkubasi 37⁰C Selama 15 Menit Pada Inkubator
5. Centrifuge 1000 Rpm Selama 10 Menit
6. Baca Hasil CrossMatch

PROCEDURE Cross Match Minor

1. Buat Suspensi Sel Eritrosit Pasien 1 % (5 ul Sel Eritrosit + 500 ul Diluent LISS)

2. Pipet 50 ul Suspensi Sel Eritrosit Pasien Masukkan Lewat Dinding Micro Tubes
3. Pipet 25 ul Serum/Plasma Donor Masukkan Lewat Dinding Micro Tubes

4. Inkubasi 37⁰C Selama 15 Menit Pada Inkubator

5. Centrifuge 1000 Rpm Selama 10 Menit

6. Baca Hasil CrossMatch

PROCEDURE Auto Control

1. Buat Suspensi Sel Eritrosit Pasien 1 % (5 ul Sel Eritrosit + 500 ul Diluent LISS)

2. Pipet 50 ul Suspensi Sel Eritrosit Pasien Masukkan Lewat Dinding Micro Tubes
3. Pipet 25 ul Serum/Plasma Pasien Masukkan Lewat Dinding Micro Tubes

4. Inkubasi 37⁰C Selama 15 Menit Pada Inkubator

5. Centrifuge 1000 Rpm Selama 10 Menit

6. Baca Hasil Auto Control

MA MI AC(autocon
(mayor) (minor) trol)

Donor Erythrocyte
50µl
Suspension

Patient Erythrocyte
50µl
50µl
Suspension
Patient Serum/ Plasma
25µl 25µl

Donor Serum/ Plasma 25µl