THE ISOLATION AND IDENTIFICATION OF Neisseria meningitidis CSF SPECIMEN

CSF SAMPLE

INOCULATE: ACID FAST BACILLI STAINING GRAM STAINING

Primary Medium: BAP, CAP
Selective and Differential Medium: MC, TMA
Maintenance Medium: NB, TSB, NA AFB NEGATIVE Gram-negative diplococci,
coffee bean-shaped

INCUBATE:
18-24 hrs at 37◦C

COLONIAL MORPHOLOGIES:
IDENTIFICATION OF ISOLATE: ANTIBIOTIC SUSCEPTIBILITY TESTING
BAP: round, smooth, moist convex with a Neisseria meningitidis
defined edge; grey and unpigmented Cephalosporin
CAP: large, no color to grey, opaque Penicillin G
Ampicillin
MC: clear and colorless Chloramphenicol
TMA: large, smooth, round, convex, no color Fluoroquinolone
RESULTS:
Aztreonam
to grey, opaque
Kovac’s Oxidase: Negative Rifampin
Ceftriaxone
NB, TSN, ThB, BHI: no growth
Dnase: Negative Minocycline
Spiramycin
Superoxol: Negative

BIOCHEMICAL TESTING: Nitrate Reduction: Negative

Kovac’s Oxidase, Dnase, Superoxol, Nitrate
Reduction, Tributyrin hydrolysis, CTA sugar, Tributyrin hydrolysis: Positive
Beta-galactosidase
CTA sugar: Glucose (+), Sucrose (-)

Maltose (+), Lactose (-)

INCUBATE: Beta-galactosidase: Negative
18-24 hrs at 37◦C