LABORATORY ASSESSMENT OF THE EFFICACY OF A

FABRIC TREATMENT TO CONTROL HOUSE DUST MITES

CUSTOMER DEVELOPMENT
BOWI STYL / RECTICEL

Sample: E 2102/06
Material: 99% PES / 1% ELASTHANE
Treatment: GREENFIRST® 2% PROFYL NK 10 (Batch: N 126 110)

SPONSOR:

BREYNER
685, rue Juliette Récamier
69970 Chaponnay
FRANCE

APRIL 2021 Report # 2641-E210206/0121R

1
 GOOD PRACTICES
STUDY TEC N°: 2641-E210206/0121
SPONSOR: BREYNER (69 – France)

SAMPLE: E 2102/06
Sent 22.02.2021

FACILITIES: T.E.C. 1, rue Jules Védrines, ZAC Maignon - 64600 Anglet -

France
DATES: 10/03/2021 to 21/04/2021
REPLICATES: 2
STUDY DIRECTOR: Bruno Serrano / ENSAT engineer
STUDY ENGINEE: Adeline D’Angelo / Master Chemist II
QUALITY RESPONSIBLE: Bruno Serrano / ENSAT engineer

METHODOLOGY:
This trial has used a methodology adapted from the standard NF G 39-011 which is in the appendix of the
proposed methods for biocide registration in the « Guidance on the Biocidal Products Regulation - Volume II
Efficacy – Assessment and Evaluation (Parts B&C) – Version 3.0 - April 2018 - ECHA».

ARCHIVING: 10 years, hard + electronic copies

DIFFICULTIES/DEVIATIONS: None

Bruno Serrano
Date: 21th April 2021

2
PARTICIPANTS TO THE TRIAL

Bruno SERRANO
Trial responsible / T.E.C. Director
Agronomist engineer ENSAT T84
Certiphyto DESA + Certibiocides

Marie-Paule MONTAUT
Technician
Internal formation
Certiphyto DESA + Certibiocides

Adeline D'ANGELO
Trial responsible
Master Chemist II
Certiphyto DESA + Certibiocides

Mélissa MARROT
Metrology responsible
BTS Analysis
Certiphyto DESA + Certibiocides

3
 LABORATORY ASSESSMENT OF EFFICACY OF A FABRIC TREATMENT
TO CONTROL HOUSE DUST MITES

1. PURPOSE

The purpose of this study is to assess the effect of an impregnating treatment of fabric on the development of
house dust mite’s populations (Dermatophagoides pteronyssinus) in comparison with a population no
exposed to the product.

The trial is done by deposit of dust mites on the fabrics impregnated or not with the active specialities.

Trial duration is 6 weeks, which corresponds to two development cycles of the mites.

2. HOUSE DUST MITES BREEDING

The species chosen (Dermatophagoides pteronyssinus) comes from a laboratory colony breeding on a
substrate made up of a 50/50 (mass/mass) blend of wheat sprouts and brewer’s yeast flakes graded by
sieving (size fragment lower than 1 mm). The temperature is between 23 and 25°C and the relative humidity
maintained at 75% with a sulphate ammoniac ([NH4)²SO4]) saturated solution ; the colony is kept in
darkness.

The colony comes from the Insect and Dust Mite Stored Products Laboratory of the French institute I.N.R.A.

3. MATERIALS AND METHOD

The methodology is adapted from French standard NF G 39-011.

The experimental unit is a small round arena (9 cm diameter), designed in order to allow air exchanges but
not the escape of the mites.

The arena contains:

- 0,1 g of food substrate (yeast + dust) for the feeding survival of the dust mites
- a square of the fabric on the ‘floor’ of the arena

50+/-5 mites are settled into the arenas.

Mites used are sorted to take the more actives.
2 replicates are done, including for the Untreated Control batches (cotton without treatment).

The units are separated by batches in boxes (polypropylene boxes with relative humidity level) and stored in
optimal breeding conditions (identical as in §2).

4. ASSESSMENTS

Death rates are assessed by direct counts using a binocular microscope, with the method established by
I.N.R.A.

Death rates are assessed after 6 weeks of incubation.

Death criteria is:

- are classified as ‘dead’ the mites unable to move
- are classified as ‘alive’ the mites able to move

Data will show the compared population’s evolution between the Treated and the Untreated.
Calculation of efficacy is explained on § 6.

4
5. EXPERIMENTAL UNITS

- test samples (sent the 22nd February 2021):

CUSTOMER DEVELOPMENT : BOWI STYL / RECTICEL

Sample: E 2102/06
Material: 99% PES / 1% ELASTHANE
Treatment: GREENFIRST® 2% PROFYL NK 10 (Batch: N 126 110)

- untreated sample: 100% PES.

6. RESULTS

6.1. Presentation

Data are numbers of alive mites converted in % of death rates.

As it is a comparison between Treated and Untreated batches, the calculation of efficacy is done with the:

POPULATION CONTROL COEFFICIENT = CP

CP = mites alive in Untreated - mites alive in Treated x 100

mites alive in Untreated

This data is the product efficacy coefficient.

- the closer to 0 the coefficient will be, the less efficient the treatment will be because the population will
develop at the same pace as for the untreated;
- the closer to 100 the coefficient will be, the more efficient the treatment will be by killing the dust mite’s
population and stopping its expansion process.

The table next page gives the raw data of the different experimental units.

6.2. Comments

The natural evolution of mite’s population in Untreated batches ratified the trial as it confirms the extremely
favourable conditions under which the batches are tested: the population on the untreated batches have
indeed a development factor close to 50 (close to 900 mites obtained from the original 50).
NOTE: the efficacy of the treatment was evaluated depending on the dust mite’s population reduction after 2
development cycles period (6 weeks).

Result on the efficacy of the treatment applied: 100% population reduction.

CONCLUSION

In the conditions of this trial, with the sample provided, the mites strain and the methodology used:

The test sample have proved a 100% control of the house dust mite’s populations.

_________________________

This result is submitted ot the warning in the corresponding invoice regarding the limits of the bioassays
5
RAW DATA

POPULATIONS' REDUCTION
after 6 weeks
Replicate A %reduction

Untreated 1 826 -
100% PES 2 871 -
mean 848.5 -
s-d 31.8 -
E 2102/06 1 0 100.0
2 0 100.0
mean 0.0 100.0
s-d 0.0 0.0

A = alive mites