NPTEL

 VIDEO  COURSE   –  PROTEOMICS                                                              

PROF.  SANJEEVA  SRIVASTAVA  
 
LECTURE-24

Quantitative proteomics: Stable isotope labeling by amino acids in cell culture

(SILAC)

QUIZ

Que.1. Which one of the following methods is not associated with in vivo labeling in
quantitative proteomics?

A. 13C-Media
B. 15N-Media
C. CDIT
D. SILAC

Que.2. The SILAC experiment depends on the cellular protein synthesis to incorporate
the stable isotope containing amino acids into whole proteome, for eg., arginine or
lysine which contains six ______ atoms. Please fill in the blank.
12
A. C
13
B. C
15
C. N
16
D. N

Que.3. Which one of the following statements is not correct with regards to SILAC?

A. 100% incorporation of labeled isotope

B. SILAC can be used for comparing up to 5 states in a single experiment
C. Cells behave exactly like control cell population grown in the presence of normal
amino acid
D. SILAC can be used for tissue and body fluid samples

Que.4. Name two widely used media, which are used for growing the cells prior to the
adaptation of the cells to the SILAC medium.

A. LB media
B. DMEM media
C. RPMI-1640 media
D. SB media

                              DEPARTMENT  OF  BIOSCIENCES  &  BIOENGINEERING                                                                                                                  

INDIAN  INSTITUTE  OF  TECHNOLOGY  (IIT),  BOMBAY,  MUMBAI,  INDIA   Page  1  
 NPTEL  VIDEO  COURSE   –  PROTEOMICS                                                              
PROF.  SANJEEVA  SRIVASTAVA  
 
Que.5. The peptides containing stable isotope-labeled arginine or lysine are heavier
than the peptides containing normal arginine or lysine. By how many Da do the heavy-
peptide and the normal-peptide differ?

A. 2 Da
B. 4 Da
C. 6 Da
D. 8 Da

Key:

1. A
2. B
3. D
4. B&C
5. C

                              DEPARTMENT  OF  BIOSCIENCES  &  BIOENGINEERING                                                                                                                  

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