D 10000CH407122103 Pages: 2

Reg No.:_______________ Name:__________________________

APJ ABDUL KALAM TECHNOLOGICAL UNIVERSITY
Seventh Semester B.Tech Degree Supplementary Examination June 2022 (2015 Scheme)

Course Code: CH407

Course Name: BIOCHEMICAL ENGINEERING
Max. Marks: 100 Duration: 3 Hours
PART A
Answer any two full questions, each carries 15 marks. Marks

1 a) Distinguish between prokaryotic and eukaryotic cells (5)

b) With a neat diagram describe differential centrifugation method for cell (5)
fractionation
c) Describe classification of proteins based on their function (5)
2 a) Derive Michaelis Menten equation. What is the significance of Km and Vmax? (8)
b) With suitable plots explain substrate activation and inhibition (7)
3 a) Explain reverse modulation of enzyme activity with an example. (8)
b) What is the role of lipids in human body? (4)
c) Give the difference between DNA and RNA. (3)

PART B
Answer any two full questions, each carries 20 marks.
4 a) Explain the chemical process application of immobilized enzymes (7)
b) Give three examples for synthetic supports used for covalent enzyme attachment (3)
c) How proteolytic enzymes are activated? What are the applications of proteolytic (10)
enzymes
5 a) Describe the three specific functions of Pentose Phosphate Pathway (6)
b) Give an example of an energy coupled reaction in cell metabolism (4)
c) Explain different types of passive transport in cells (10)
6 a) Explain the two types enzyme specificity hypothesis (6)
b) Describe briefly the role of dialysis in enzyme purification (4)
c) Describe tricarboxylic acid cycle in detail (10)
PART C
Answer any two full questions, each carries 15 marks.
7 a) Explain the working of a hemocytometer with a neat diagram (7)

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b) Describe the effect of substrate concentration on microbial growth and give the (8)
significance of Monad equation
8 a) Describe the gas liquid mass transfer in cellular systems (8)
b) Describe the continuous heat sterilization of liquids (7)
9 a) Explain the classification of microbial process according to Gaden (7)
b) With a neat diagram explain the working of a bioreactor (8)
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