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SIES GST

A Non-invasive way to Detect Anemia using


Machine Learning
Prof . Shyamala Mathi. Samiksha Chikka. Furqan Budye.
Dept.Electronics and Telecommunication Dept.Electronics and Telecommunication Dept.Electronics and Telecommunication
SIES Graduate School of Technology, ,SIES Graduate School of Technology, , SIES Graduate School of Technology,
Navi Mumbai, India Navi Mumbai, India Navi Mumbai, India
shyamalae@sies.edu.in samikshacextc120@gst.sies.edu.in furqansbextc221@gst.sies.edu.in

Ajaz Shaikh. Amir Mithagiri.


Dept.Electronics and Telecommunication Dept.Electronics and Telecommunication
SIES Graduate School of Technology, SIES Graduate School of Technology,
Navi Mumbai, India Navi Mumbai, India
ajazahmedssextc221@gst.sies.edu.in amirsmextc221@gst.sies.edu.in

Abstract—The challenge of anemia significantly impacts In recent years, the use of machine learning has emerged as
children and pregnant women worldwide. According to the a significant tool in healthcare, with promising outcomes in
World Health Organization (WHO), anemia affects illness detection. This study employs convolutional neural
approximately 40% of pregnant women and an alarming 42% networks (CNN) for the development of a noninvasive
of children under six years old globally. It is a real problem for screening test for anemia.and Random Forest algorithms to
approximately 33% of the entire population due to iron
identify anemia. The application of CNN enables effective
deficiency. In recent times, there has been a lot of buzz around
machine learning algorithms to deal with anemia, and it seems
extraction of features from conjunctiva pictures, which aids
kind of promising. Thus, two machine learning methods were in the detection of anemia-related abnormalities. Random
employed in this study to identify iron-deficiency anemia. by Forest, noted for its collaborative learning technique,
analyzing images of their conjunctiva. The process involves excelsin handling various datasets and making reliable
collection of data, doing some preprocessing, and developing predictions. By integrating these methods, we hope to
their models. So, the abstract talks a lot about critical improve both the precision and dependability of anemia
considerations in this field, like dataset quality and the pesky
identification while resolving the constraints.
issue of model interpretability. We need more research to refine
and validate these models across diverse populations and This study uses a Virtual reality (VR) anaemia detection
healthcare settings. It's advocating for collaboration between presents a new method for medical diagnostics. The goal of
data scientists, medical professionals, and policymakers. Using this research is to increase accuracy and user experience.
machine learning with non-invasive techniques for anemia
for anemia detection through the integration of VR
detection is a new technique in healthcare. It's like an
technologies. Through the use of virtual reality
advancement in human life . It's all about revolutionizing the
way we diagnose, manage, and monitor anemia. And as environments, people can partake in immersive experiences
technology keeps progressing and we get more and more data, it that mimic different diagnostic circumstances, like
is going make millions of lives better. Early detection, evaluating photographs of the conjunctiva and also creates a
personalized treatment, efficient healthcare delivery becomes control environment for anaemia detection. The study aims
easy. As a result of our efforts, we have designed a computerized to identify minor signs of anemia by utilizing the optical and
screening test that is noninvasive, easy to use, and affordable. cognitive capabilities of virtual reality through this novel
This innovative test is portable and user-friendly, making it approach. Users can explore virtual worlds, look at 3D
accessible for widespread use in developing nations. By offering models of blood components, and take part in mock
a practical substitute to invasive techniques for detecting diagnostic exercises. The intention is to develop a more user-
anemia, this screening test has the potential to significantly friendly platform that will help medical professionals and
improve the quality of life for individuals in these regions. Its even the general public recognize possible cases of anemia.
simplicity and affordability make it a valuable tool in addressing
healthcare challenges faced by populations in resource-limited
settings. This advancement represents a step forward in
providing essential healthcare solutions tailored to the needs of II. LITERATURE SURVEY
underserved communities. Numerous scholars and students have published relevant
research in national and international journals, theses, and
Keywords— iron deficiency, Anemia, non-invasive, Machine papers. Their work aims to understand the objectives, explore
Learning, image processing ,detect , user friendly , Healthcare , the range of algorithms employed, and investigate various
methods for pre-processing and data analysis
I. INTRODUCTION
Anemia, a common worldwide health condition, In a study published in 2023 by Emmanuel Timmy Donkoh,
disproportionately affects vulnerable groups including kids Giovanni Dimauro, and Rosalia Maglietta, non-invasive
as well as pregnant women. Early and correct identification methods utilizing machine learning (ML) algorithms were
of anemia is critical for successful intervention and therapy. explored for anemia detection, focusing on palm images as an
alternative to conjunctiva images. The methodology consisted
of three phases: dataset collection involving palm images, hemoglobin concentration and Hb coefficients. The sampled
preprocessing including image extraction, augmentation, ROI data's percentage average relative deviation was 0.43% [3].
segmentation, and analysis in the CIELAB color space, and
model development using Convolutional Neural Networks In their experiment, Pooja Tukaram Dalvi and Nagaraj
(CNN), K-Nearest Neighbors (K-NN), Naive Bayes, Sum, Verenkar suggested an Ensemble Learning Technique and
and Decision Tree algorithms. The CNN model was evaluated classification performance using 10-fold cross-
configured with AlexNet architecture, optimized with validation. 50 subsets of the dataset were created, with one
Stochastic Gradient Descent (SGD), ReLU activation subset being used for testing and the other 49 for training.
function, regularization (a coefficient of 0.0001), and 10 Based on the results, it was shown that Artificial Neural
iterations. Data processing involved converting the ROI into Network (ANN) outperformed Decision Tree, Naïve Bayes,
the CIELAB Color space to mimic human visual perception, and K-NN in the classification of red blood cells (RBCs). The
with criteria set for high (a* > 160) and low (a* < 142) best accuracy, specificity, recall, and precision were notably
hemoglobin values. Results showed high accuracy with Naive shown by a stacked classifier that included K-NN and
Bayes achieving 99.66%, Sum algorithm 96.39%, and CNN Decision Tree. The classifier ensemble using Decision Tree,
the highest at 99.92%. Image augmentation was highlighted K-NN, Naïve Bayes, and ANN combined yielded the best
as a key technique, increasing the dataset size from 527 to accuracy in voting. In general, stacking performed the best,
2635 images,enhancing model robustness for anemia followed by voting, bagging, Bayesian boosting, and
detection. [1]. boosting. ANN frequently generated superior outcomes than
Peter Appiahene1, Justice Williams Asare proposed a study all other ensemble approaches, followed by Decision Tree and
which evaluated machine learning models for iron-deficiency Naïve Bayes, and K-NN [4].
anemia detection using The accuracy, F1-score, AUC,
precision, recall, and machine learning models for anemia due Azwad Tamir1, Chowdhury S. Jahan1, Mohammad S. Saif
to iron deficiency identification were assessed in this work. proposed a study using RGB thresholding to detect anemia in
With an accuracy of 98.96% on the palpable palm, the Naïve anemic patients. The white sclera and anterior conjunctiva are
Bayes model outperformed the Convolutional Neural extracted, and the RGB spectrum is standardized using the
Network (CNN) at 99.12%. The Support Vector Machine white sclera part's brightness. The red and green color
(SVM) produced the least accurate findings, with 95.34% intensities are compared to determine anemia. A threshold
accuracy.The models' results on the dataset of nail color were value of 1.5 is selected based on previous eye images,
comparable. For this purpose, the palm is a reliable indicating a small difference between anemic and non-anemic
anatomical feature of the human body, and the CNN model patients. A Redmi Note 3 pro cellphone was used to take
outperforms the Naïve Bayes. In order to diagnose iron pictures of 19 patients, and the blood report was used to
deficiency anemia, the outcome of machine learning models extract the patients' hemoglobin levels. The system accurately
(k-NN, Naïve Bayes Decision Tree, CNN, and SVM) was anticipated 15 from the 19 values, resulting in an accuracy of
examined in this study. To train, validate, and test the system, almost 78.9%, according to the data. The patients' anterior
images of the ocular conjunctiva, palpable palms, and nail conjunctival pallor red and green spectrums were then
color were employed. When tested on the photos of the evaluated by the algorithm. The findings demonstrated that
conjunctiva, palpable palm, and fingernails, the CNN non-anemic individuals had a greater intensity of the red
outperformed all the other models in terms of accuracy. spectrum while anemic patients had a greater intensity of the
Furthermore, when tested using the data set, the palm models green spectrum relative to red. The patient's condition is then
performed more accurately than the fingernails and ascertained by the algorithm by averaging the two numbers
conjunctiva models. As proven by the models' outcomes the and comparing them. The patient is classified as non-anemic
palpable palm is one of the most reliable human warning signs if there is a difference among the two spectrums that is more
of anemia, and CNN is resilient and beats Naïve Bayes, than the threshold value [5].
Decision Tree, k-NN, and SVM in this domain. [2].
The study aims to predict anemia in children under 5 years
The authors J.A.D.C.A. Jayakody, E.A.G.A.Edirisinghe used using classifier algorithms proposed by Prakriti Dhakal,
a methodology to train a machine learning algorithm for Santosh Khanal, and Rabindra Bista. Experiments were
anemia diagnosis. They used training data from patients conducted on unbalanced and balanced datasets. Random
diagnosed with anemia and healthy individuals to train the Forest achieved 98.4% accuracy for unbalanced data.
algorithm. The server played a significant captured role in Ensemble learning methods like Stacking and Bagging
obtaining inputs from these data sets, increasing the chances improved accuracy to 98.8%. Stacking Random Forest with
of accurate results. The algorithm was trained using SVM, Naïve Bayes, Logistic Regression achieved accuracy
supervised machine learning, initially feeding the output to of 98.7% and Extreme Gradient Boosting led to 99%
the system. After being sorted, the gathered data was utilized accuracy. Other ensemble methods did not show significant
to create the algorithm. After that, the computer evaluated the improvement [6].
photos of the fingertip and a questionnaire from a mobile app
to produce results relating to anemia. By comparing the non- Matthew Bihis, Paul Hage, and Johnny Ren conducted a study
invasive measures with traditional blood samples, utilizing concentrated facial pallor site photos. Their research
HemoSmart was able to demonstrate a linear link between focuses on employing image-based techniques for feature
extraction, categorization, and data modeling to screen and regulates the light strip to provide consistent illumination
classify anemia-like pallor. The research highlights the when taking pictures. Fit the Raspberry Pi with the trained
significance of particular spatial Regions with Interest (ROIs) CNN and Random Forest models for real time analysis.
and features based on picture intensity in differentiating
5. VR 3D Modeling:
between normal and aberrant images. The inner tongue and
conjunctiva regions of the eye are important for Create the VR environment, using a 3D modelling program
distinguishing between normal and aberrant images. The (such as Blender or Tinkercad). Create virtual models for
technique is able to test for anemia severity utilizing face conjunctival photos
pallor site photos, as evidenced by its 86% screening accuracy
for eye images and 98% screening accuracy for tongue images
[7].

III. SYSTEM ARCHITECTURE


The proposed system architecture aims to improve non-
invasive anemia detection by integrating advanced
technologies like Convolutional Neural Networks (CNN)
and Random Forest algorithms. It involves dataset
acquisition, cleaning, hardware interfacing with a Raspberry
Pi 4B Plus, camera module, and light strip, and 3D modeling
for virtual reality simulations. The system's primary
objective is to enhance anemia detection by leveraging a
diverse dataset of conjunctiva images. The system's hardware
Fig1.Proposed system architecture for algorithm
integration enables real-time analysis and provides an
immersive VR experience, enhancing user engagement and This comprehensive system architecture combines image
transforming the diagnostic process. processing, machine learning, hardware interfacing, and
virtual reality to create a non-invasive anemia detection
1. Dataset Acquisition:
system. It addresses dataset acquisition and cleaning,
Gather a range of conjunctiva images illustrating both
algorithm implementation, hardware integration, and the
anemic and non-anemic situations. From the medical
development of an immersive VR environment
institutions, make use of datasets that are accessible to the
public, or gather data using cameras.
IV. PRINCIPLE OF VIRTUAL
2. Cleaning the dataset; REALITY
Utilize preprocessing techniques to enhance image quality.
and standardize their formats. Verify that photos are
The primary research question is "How to detect anemia
accurately labelled as anemic or non-anemic.
(deficiency of hemoglobin in an individual's blood) without
the use of conventional blood tests. "VR FOR ANAEMIA
3. Random Forest Algorithm and CNN:
DETECTION" is a noninvasive tool designed to address the
For feature Extraction (CNN): Extract pertinent features previously mentioned issue of simple anemia detection.
from conjunctiva pictures by applying Convolutional Neural
Virtual Reality (VR) is a powerful tool for anemia
Networks.
detection, providing an immersive and interactive simulated
For Classification (Random Forest): Using the collected environment for healthcare professionals to visualize and
characteristics as a basis, use the Random Forest method for analyze diagnostic indicators. Key principles include
robust classification. To maximize the performance of both immersive visualization, interactive exploration, real-time
models, train them using the preprocessed dataset. feedback, and standardized conditions. VR provides a three-
dimensional, detailed environment, allowing healthcare
4. Hardware Interfacing with 5MP Raspberry MB
professionals to visualize conjunctiva images and related
Camera Module, 5V SMD Light Strip, and Raspberry Pi 4B
diagnostic information. It also allows users to actively
Plus:
engage with the virtual environment, participate in simulated
The Raspberry Pi 4B Plus: It act as the main processor. diagnostic tasks, and receive immediate feedback on the
likelihood of anemia. The integration of machine learning
5MP Raspberry MB Camera Module: It takes the pictures of
models like CNN and Random Forest provides decision
the conjunctiva for examination.
support, assisting healthcare professionals in making
5V SMD Light Strip: To Make sure the illumination is informed diagnoses. VR also minimizes external
constant so that pictures may be taken. distractions, allowing healthcare professionals to focus on the
diagnostic task at hand. The user-friendly interface, with
Steps for Interfacing: To acquire images, connect the camera
intuitive controls and efficient interaction, ensures seamless
module to the Raspberry Pi. Put in place a script that
navigation and improved diagnostic skills.
V. DESIGING OF VR show color fluctuations, which is important for
identifying any anemia indications. Their capacity to adjust
In this paper, the process of creating a virtual reality
to different color temperatures guarantees that the lighting
platform specifically for anemia detection is started.The
may be adjusted to maximize the accuracy of the diagnostics.
subsequent parts will elaborate on the technique,
SMD LEDs' efficient and low-heat properties enhance users'
technological concerns, and potential impact of this inventive
overall comfort during prolonged VR sessions, which is
approach in reforming healthcare procedures, as we delve
crucial for applications in healthcare vital for medical
into the complexities of VR design for anemia identification.
applications requiring in-depth, targeted analysis. The
A. Selecting a VR Microprocessor strategic integration of cool white SMD LEDs is in line with
the objectives of dependability, clarity, and user-centered
It is important to take into account aspects like design, as it advances the field of anemia detection via virtual
processing power ,efficiency, compatibility with VR gadgets, reality technology.
and support for real-time image processing when choosing a
therefore , a 12V Cold White SMD led strip was selected
microprocessor for a Virtual Reality (VR) system,
after taking these variables into account.
particularly for anemia detection.
Finally, Raspberry 4 model B was selected because of the D. Interfacing the Electronic Components
Versatility and enhanced Graphics Performance,faster wired
network connectivity when compared with the wireless A comprehensive and effective system is created by
raspberry pi integrating components such as the Raspberry Pi 4 Model B,
the Raspberry Pi Camera Module, and cold white SMD LEDs
B. Appropriate Camera Selection into the electronic circuit for anemia diagnosis through
virtual reality (VR). The central processing unit is a
The main goal in choosing a camera for Virtual Reality Raspberry Pi 4 Model B, which manages data flow and runs
(VR) anemia diagnosis is to offer the best achievable imaging image processing algorithms. The conjunctiva of the eyes is
quality and accuracy, which will allow for the precise captured in fine detail by the Raspberry Pi Camera Module,
recording and examination of faint physiological markers in allowing for accurate analysis in search of anemia signs. Cold
the conjunctiva of the eyes. The selected camera must meet white SMD LEDs that are included into the circuit provide
the unique specifications of the VR system for detecting maximum visibility and precise detection by lighting the
anemia. conjunctiva during virtual reality tests. By connecting the
● Infrared camera Raspberry Pi 4 to the camera module, these components
● Cell phone camera were interfaced to enable real-time image capturing and
● Web camera synchronize the activation of SMD LEDs for even and
● Raspberry 4 model B camera constant lighting. Together, these parts form a potent
electronic circuit that synchronizes illumination, image
Finally 5MP Raspberry 4 Model B camera was was capturing, and data processing, enhancing the efficacy of
opted to make our VR anemia detection in a virtual reality setting
C. System Design for Lighting E. Constructing a functional prototype
In order to obtain an accurate picture of the conjunctiva for
In order to create an interesting and practical diagnostic tool,
detailed imaging, key considerations when selecting a light
an integrated strategy is taken when constructing a prototype
strip for a Virtual Reality (VR) system included flexibility,
for Virtual Reality (VR) anemia diagnosis. Hardware and
luminosity, hue accuracy, visibility, eliminating discomfort,
software elements are combined; In order to integrate the
delivering accurate illumination, compatibility with VR
electronic components, a 3D model was printed.
environment, and convenience of integration.

Factors to be taken into consideration :


1) Luminosity of light
2) Hue of light
3) the separation between the eye's
conjunctiva and light strip
4) Low Heat Emissions
When it comes to the field of Virtual Reality (VR) for
anemia detection, cold white SMD LEDs are a crucial Fig .2 of conjunctiva for running ML algo Fig .3 of VR
element in producing a diagnostic environment that is both
efficient and engaging. In applications such as conjunctiva
examination, cold white SMD LEDs provide a high F. Gathering Dataset and Creating an Algorithm
temperature of color and produce a bright, crisp illumination
that facilitates the visibility of minor physiological Biomedical scientists in Ghana were trained to capture
indications. These LEDs' cold white light helps to accurately conjunctival images from children aged 6-59 months using
electronic instruments and mobile tablets. The system
collected biodata, Hb levels, age, and gender, and took
images of the conjunctiva of the eyes. The images were taken
in ambient natural light, with spotlights turned off to prevent
excessive shine effects. The ROI of the conjunctiva was
extracted using the triangle thresholding algorithm and
entropy grayscale image algorithm. The dataset was taken
from 10 hospitals present in Ghana
Because of their complementing advantages,
Convolutional Neural Networks (CNN) and the Random
Forest method are frequently used in conjunction for the
identification of anemia. CNNs are excellent at extracting
fine-grained characteristics from images, which makes them
useful for tasks like conjunctiva evaluation, where minute
visual clues could be signs of anemia. In order to detect
anemia in eye pictures, CNNs can automatically develop
hierarchical feature representations, collecting both high-
level patterns and low-level details. By merging several
decision trees, the ensemble learning algorithm Random Fig .4 Flowchart of Convolutional Neural Network + Random Forest
Forest is excellent at producing precise predictions. It
enhances generalization and decreases overfitting.
The interpretability that Random Forest makes it simpler G. Completed Device
to comprehend the logic underlying the predictions. This is To set up the system a VNC viewer app on cellphone was
important since interpretability is frequently a major concern connected with the PC .The locally hosted online application
in medical applications. It is especially useful to combine using the VNC viewer, included ocular conjunctiva photos
CNNs with Random Forest when working with little amounts which was taken by the camera module set up in VR for the
of labeled data. From the provided images, CNNs are able to web interface . For the anemia detection; algorithm of CNN
learn robust representations, and Random Forest performs and Random Forest. The CNN's extracted characteristics are
well when it comes to generalizing the learnt features. CNNs then fed into a Random Forest classification algorithm.
ensure strong feature extraction by handling a variety of
visual circumstances, including lighting and orientation.
Random Forest improves the model's flexibility to various An ensemble learning technique called Random Forest uses
scenarios by taking into account a variety of decision routes, decision trees to categorize data. In this situation, it predicts
which aids in generalization. Modeling becomes more whether the image shows the existence of anemia or not. The
diverse when CNN and Random Forest are combined. Random Forest classifier predicts each image based on the
Modelling becomes more diverse when CNN and patterns and attributes it has learned. These predictions show
Random Forest are combined. Due to Random Forest's the possibility that anemia is present in the image. The
ensemble approach, CNNs are less likely to overfit when prediction findings, together with the associated photos, are
faced with sparse data, which results in a more stable and shown on the locally hosted web interface.
dependable model. By enabling feature importance analysis,
Random Forest offers insights into which features—learned The online program was interacted remotely via the mobile
by CNN—have the greatest bearing on the decision-making phone's VNC viewer, which allows you to take ocular
process. Understanding the importance of various visual conjunctiva images and receive real-time anemia detection
clues in the identification of anemia may depend on this. In results. This provides a user-friendly interface accessible
conclusion, the combination of CNN and Random Forest remotely via a mobile phone to detect anemia.
capitalizes on the advantages of both algorithms, overcoming
obstacles such interpretability issues, overfitting, and data
scarcity to eventually improve the efficacy of anemia
diagnosis in a medical setting. Fig .4 illustrates the flow chart
of CNN and Random Forest
accuracy of CNN being the lowest with 49% . Logistic
Regression had accuracy of 89% but the F1 score and
Precision was relatively very low. VGG+RF had the accuracy
of 88.53% and XGBoost with 90.48% almost the same
percentage but CNN+RF outweighed all the algorithms
There are various benefits of combining the Random Forest
(RF) method with Convolutional Neural Networks (CNNs)
for the identification of anemia. CNNs are an effective tool
for image recognition tasks. Through automatic feature
extraction from the images, they are able to identify complex
patterns that may be suggestive of anemia. The capacity of
CNNs to extract complex features from images and the
resilience of RF in handling high-dimensional data and
Fig .5 Functional schematic of the complete project for Wireless setup preventing overfitting can both be utilized by merging these
The system knows how to produce the intended output even two methods. When integrated, these methods can produce
while it is unaware of the final outcome. In the project, this machine learning models for anemia detection that are more
design was put into practice. Relevant data sets were fed into reliable and accurate.
the output to train the algorithm and enable it to determine the A table is shown between the presence of anemia in
potential consequences of the inputs fed into it. By blood sample and the VR based anemia prediction which was
integrating these technologies, the system enables efficient non-invasively measured is given below .
and accurate screening for anemia, facilitating early detection
TABLE I. a collection of patients with real anemic states and our VR
and intervention to improve patient outcomes .
Fig 5 illustrates the schematic diagram of whole diagram
Patient No: Age Presence of VR-based
anemia based anemia
VI. RESULTS AND on a blood prediction
sample
DISCUSSION
1 20 No NO
A dataset of 710 images of the ocular eye conjunctiva 2 22 Yes Yes
from Ghana was fed into our machine learning algorithm; 3 19 No NO
60% of the images were identified as anemic, while 40% were
not.An algorithm evaluates the ocular conjunctiva image that 4 20 No NO
is taken when a patient uses the device, using the information 5 20 No NO
to determine whether or not the patient has anemia
6 22 Yes No
7 23 NO NO
8 21 NO NO
9 23 NO NO
10 24 Yes NO

The created non-invasive device had a covered design and


was intended to be worn. Choosing a covered design made
logical for a number of reasons.

Reason 1: Eliminating ambient light is necessary to avoid the


excessive shine effects caused by poor picture quality, which
have a significant impact on the models' ability to detect and
Fig .6 Performance Metrics of Different Algorithms with classify objects and features. The technique of creating a
Accuracy ,F1 Score and Precison controlled environment works well for removing light from
the surroundings from photos while building datasets.
After testing five alternative algorithms VGG16 and Random Reason 2: As we worked on the Ghana dataset, we discovered
Forest, CNN, XGBoost, Logic Regression, and Convolutional that the conjunctiva's paleness varies by region. This, along
Neural Network with Random forest using the metrics of with the possibility that all of the images were taken with a
accuracy, F1 score, precision, and, it was discovered that mobile phone in a variety of environmental settings, led us to
CNN in conjunction with Random Forest had the highest the conclusion that all of this can be resolved by using an
accuracy of 98.44 % as shown in the above graph along with enclosed virtual reality device, which will help to capture
the highest F1 score and precision. With consistent images leading to a more precise anemia detection
method, enabling the creation of a diversified or any other invasive anemia detection gadget that was developed would
region-specific dataset make it easier for people to identify anemia and treat it before
it worsens.
In rural locations like Ghana, the finger prick test for
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