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Yakeen NEET 2.0 (Legend)

Biotechnology: Principles and Process DPP-05

1. Arrange the following steps in order 6. Spooling is

A) Restriction Digestion (1) Amplification of DNA
B) Running Agarose gel (2) Cutting of separated DNA bands from the
C) Elution of bands agarose gel
D) Purification of DNA (3) Transfer of separated DNA fragments to
E) Ligation synthetic membranes
F) Transformation (4) Collection of isolated DNA
G) Cloning
(1) A-B-C-D-E-F-G (2) A-E-F-G-B-C-D 7. A mixture containing DNA fragments, a, b, c and
(3) A-B-C-E-F-G-D (4) B-C-D-A-E-F-G d, with molecular weights of a + b = c, where a >b
and d > c, was subjected to agarose gel
2. Significance of ‘heat shock’ method in bacterial electrophoresis. The positions of these fragments
transformation is to facilitate from anode to cathode sides of the gel would be
(1) Binding of DNA to the cell wall (1) b, a, c, d (2) a, b, c, d
(2) Uptake of DNA through membrane transport (3) c, b, a, d (4) b, a, d, c
proteins
(3) Uptake of DNA through transient pores in the 8. Criterion for DNA fragments movement on agarose
bacterial cell wall and plasma membrane gel during gel electrophoresis is
(4) Expression of antibiotic resistance gene (1) The larger the fragment size, the farther it moves
(2) The smaller the fragment size, the farther it
3. Method in which recombinant DNA is directly moves
injected into the nucleus of animal cells by using (3) Positively charged fragments move to farther end
microneedles is called (4) Negatively charged fragments do not move
(1) Gene gun method
(2) Biolistic method 9. In a DNA gel, the fragment of 2kb and 3kb will be
(3) Microinjection method (1) 2kb nearer to cathode while 3kb nearer to
(4) Indirect method anode
(2) 2kb closer to wells while 3kb towards the
4. Biolistic (gene gun) is suitable for opposite ends
(1) Disarming pathogen vectors (3) 2kb closer to opposite end of gel while 3kb
(2) Transformation of plant cell closer to well
(3) Constructing recombination DNA by joining (4) 2kb away from cathode while 3kb towards
with vectors anode
(4) DNA fingerprinting
10. The DNA fragments separated on an agarose gel
5. Method involving entry of high velocity gold micro can be visualised only after staining the DNA with
particles coated with DNA into plant cell is a compound known as ____.
(1) Protoplast fusion (1) acetocarmine
(2) Transfection (2) aniline blue
(3) Biolistics (3) ethidium bromide
(4) safranin
(4) Magic bullet action
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11. During isolation of DNA, addition of _________ 13. Which of the following is incorrectly matched?
causes precipitation of purified DNA (1) Agarose - Sea weeds
(1) Chilled ethanol (2) Thermus aquaticus - T-DNA
(2) Ribonuclease enzyme (3) Plasmid DNA - Vector
(3) DNA polymerase (4) Sal I - Restriction endonuclease
(4) Proteases

12. The key tools required for the recombinant DNA

technology are
I. Restriction enzymes
II. Host organisms
III. Ligases
IV. Vectors
Select the correct option
(1) I, II & III
(2) I, III, IV
(3) I, II, & IV
(4) I, II, III. IV
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Note: Kindly find the Video Solution of DPPs Questions in the DPPs Section.
Answer Key
1. (1) 8. (2)
2. (3) 9. (3)
3. (3) 10. (3)
4. (2) 11. (1)
5. (3) 12. (4)
6. (4) 13. (2)
7. (1)

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