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Yakeen NEET 2.0 (Legend)

Biotechnology- Principle and Processes DPP- 01

1. Plasmid is a (3) Recognise a specific nucleotide sequence for

(1) Fungus binding of DNA ligase
(2) Protozoans (4) Restrict the action of the enzyme DNA
(3) Part of plasma membrane polymerase
(4) Extra chromosomal DNA in bacterial cell
7. Identify the plasmid
(1) pBR 322 (2) Hind II
2. Two microbes found to be very useful in genetic
(3) EcoR I (4) AIU I
engineering are
(1) Escherichia coli and Agrobacterium tumefaciens 8. Consider the following statements
(2) Vibrio cholerae and tailed bacteriophage I. Asexual reproduction preserves genetic
(3) Diplococcus sp. and Pseudomonas sp. information while sexual reproduction permits
(4) None of the above variations
II. Traditional hybridisation often leads to the
3. First recombinant was constructed in the year inclusion and multiplication of undesirable
(1) 1963 (2) 1974 genes along with the desired genes
(3) 1918 (4) 1972 III. rDNA technology allows us to isolate and
introduce only one or a set of desirable genes
without introducing undesirable genes in the
4. The cutting of DNA at specific locations became
target organism
possible with the discovery of_____ Which of the above statements are true?
(1) Restriction endonuclease enzymes (1) I & II only
(2) Probes (2) I & III only
(3) Selectable markers (3) II & III only
(4) Ligases (4) I, II, & III

5. Term ‘Restriction’ in restriction endonuclease 9. The enzyme that seals 5' PO4 and 3' OH
enzyme refers to polynucleotide ends while creating a recombinant
DNA molecule is
(1) Cleaving of phosphodiester bond in DNA by the
(1) Alkaline phosphatase
enzyme
(2) DNA ligase
(2) Prevention of bacteriophage multiplication in (3) DNAse
bacteria (4) Restriction endonuclease
(3) Cutting of DNA at specific position only
(4) Cutting each of the two strands of DNA at 10. Which of the following is a palindromic sequence?
specific points in sugar phosphate backbone (1) 5'-CGTATG-3’
3'-CGAATG-5’
6. Restriction endonucleases are enzymes which (2) 5'-CGAATG-3’
(1) Remove nucleotides from the ends of the DNA 3'-GCATAC-5’
molecule (3) 5'-GAATTC-3’
3'-CTTAAG-5’
(2) Make cuts at specific positions within the DNA
(4) 5'-GACTAC-3’
molecule
3'-CTTAAG-5'
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11. The DNA molecule to which the gene of interest is 14. Which of the following is related to genetic engineering?
integrated for cloning is called (1) Heterosis
(1) Template (2) Mutation
(2) Carrier (3) Plastid
(3) Transformer (4) Plasmid
(4) Vector
15. Plasmid has been used as vector because
12. The cutting of DNA at specific locations became (1) Both its ends show replication
possible with the discovery of _______ (2) It can move between prokaryotic and eukaryotic cells
(1) Selectable markers
(3) It is circular DNA which have capacity to join to
(2) Ligases eukaryotic DNA
(3) Restriction enzymes
(4) It has antibiotic resistance gene
(4) Probes

13. Which of the following are used in gene cloning?

(1) Nucleoids (2) Lysosomes
(3) Mesosomes (4) Plasmids
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Note: Kindly find the Video Solution of DPPs Questions in the Quiz Section
Answer Key
1. (4) 9. (2)
2. (1) 10. (3)
3. (4) 11. (4)
4. (1) 12. (3)
5. (2) 13. (4)
6. (2) 14. (4)
7. (1) 15. (3)
8. (4)

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