METHOD OF ANALYSIS OF TRANSFTUTHRIN

L.6%LV
A) Method of Anatysis of Determination of purity
Transfruthrin Tech By GC
of
scope : This method is applicable for Analysis
of Transfluthrin (Tech) as a Raw Material
Objective : To find out actuat purity of Transfluthrin (Tech).
Apparatus : 1) Gas chromatograph with Hp_5
Capillary Cotumn.
Reagents : 1) Acetone A.R.Grade

2) Reference Standard of known purity

3) Din_ butyt Phthatate (DBP)

Sampling of R.M.: As per sampling plan

Physical Appearance :Take approx. 5.0

ml melted Test sampre in a crean
dry test tube and inspect
visually for colour clarity and extraneous particles.

B) Determination of Assay by GC

l.Apparatus:
Gas chromatograph with equipped with a hydrogen flame ionization
detector and coupled to an
integrator cum printer -plotter shall be
used with the following operating
conditions.
Column : HP-Scapilrary corumn with 30
meter rength and 0.32 mm rD
Microlitre syringe : 10 micro liter syringe, manual.
lnjection volume : 1 microlitre
Temperatures: lnjector port :250" C
Column port : 200'C
Detector port :260" C
Gases and corresponding ftow rates.
(carrier)
Nitrogen :1.0 mllmin
Hydrogen :30 ml/min
Air
w
r'or Hindustaa Agfo
:300 m/min

Prooricto!

HINDUSTAN AGRO
An ISO 9OO 1 t ?O1 5 Cerrified Gom pany
Haridwar Road Mandawar, District Bijnor; U.p. India (24672r)
Email : info@hindustanagrotech,com, hindustanagrolT@gmail.com lWeb.: **w.hindustanagrotech.com
Contact : +91-8859497996, 9720553875
GSTIN : O9CXIPS6919R1Z7 | tEC CODE : CXIpSGgIgR
 2.Reagents: Acetone ( A.R.Grade )
Reference std.of known purity.
Dibutyl phthalate (internal Std.) A.R. Grade

3.Procedure:
Preparation of std. stock solution (solution A):- weigh approx. 500
mgs of std. Transfluthrin in a 100 ml
volumetric flask and make the votume 100 ml with Acetone. This stock
Solution A.
Preparation of lnternal standard stock solution (solution B):- weigh
approx. 500 mgs of Di butyt
phthalate in a 100 ml'volumetric flask and make the volume
100 ml with Acetone. This stock solution B.
Preparation of working standard solution: - pipette out 5.0 ml
of solution A and 5ml sotution B in a
clean 100 ml volumetric flask & make up the volume with Acetone.

Preparation of sample solution:- similarly weigh 500 mgs accurately

weighed to 0.0001 gm. of the
sample in a 100 ml volumetric flask. & make up the volume with Acetone.pipette out 5 ml of above
sample solution in a clean dry volumetric flask, add 5 ml of solution
B and make up the volume with
Acetone.

4.Estimation:
lnject 1'0 microlitre of the standard solution with the help of a lomicrolitre
syringe. Measure the peak
area ofthe internal Standard and Transfluthrin Tech. in the standard
solution.
similarly inject 1 microliter of the sample solution with the help of a 10
microlitresyringe. Measure the
peak area of the internal standard and Transfluthrin Tech.
in the sample and calculate the contents as
described below.

5.Calculation:-

Active Transfluthrin Tech. Contefito/o = M1x A1x 43 x P1

M2x fr)Y X4

9"Y
HINDUSTAhI AGRO
An ISO 9OO 1 t 2O1 5 Gertified Gompany
Haridwar Road Mandawar, District Bijnor, U.P. India (24672L)
Email : info@hindustanagrotech,com, hindustanagrolT@gmail.com lWeb.:www.hindustanagrotech.com
Contact : +9 1-8859497996, 972055387 5
GSTIN : O9CXIPS6919R1Z7 | tEC CODE : CXIPS6919R
 Where:-
M1 = Weight in mg of the standard Transfluthrin Tech. in 5 ml
41 = Peak area of internal standard in the standard solution
A3 = Peak area of Transfluthrin Tech. in the sample solution
M2 = weight in the mgs of Transfluthrin Tech. in 5 ml sample solution
42 = Peak area of Transfluthrin Tech. in the standard solution
44 = peak area of the internarstandard in the sampre sorution
P1 = Percent purity of reference standard Transfluthrin Tech.

c) METHOD OFANALYSTS PERFUME :

a) Determination of physicat Appearance

Physical Appearance :Take approx. 5'0 ml Test sample in
a ctean dry test tube and inspect visually for
colour clarity and extraneous particles

b) Determination of Density by pychnometer

l.Scope: These methods are applicable for Analysis of perfume

as a Raw material for LV
2. objective : To find out appearance and to determine the density of it

3. Apparatus : 1) Test tube

2) Pychnometer

) Weigh accuretly empty pychnometer for Density

=A
) Fill the Pychnometer with water
.F Note down the weight of pychnometer + Water
= B

) Dry the pychnometer and note down weight of Pychnometer + perfume sample = c
F Ensure the leval of liquid for both water as wert as perfume

c-A
Density = x 0.998
B-A
fior Hindustaa Agro
Sa/r,otL
Proorictor 1i. ,

HINDUSTAN AGRO
An ISO 9OO 1 t 2O1 5 Gentified Gompany
Haridwar Road Mandawar, District Bijnor, U.P. India (246721)
Email info@hindustanagrotech.com, hindustanagrolT@gmail.com lWeb.: www.hindustanagrotech.com
Contact : +9 1-8859497996, 9720553875
GSTIN : O9CXIPS6919R1Z7 | IEC CODE : CXIPS5919R
 D) METHOD OFANALYSTS FOR DOK

a) Determination of PhysicalAppearance : Take approx. 5.0 mlTest sample

in a clean Glass Test tube
and inspect visually for colour and extraneous particles.

b) Determination of lO% Aqs. Solution pH

1. Take 10 mlTest sample in 250 mt Beaker.

2. Add to it 90 ml water and stir to homogeneous mixture

3. lf it is Clear Solution check the pH by pH electrode.

c) Determination of Density

Scope :This method is applicable for Analysis of DoK as Raw material

objective : To find out actual purity of DoK and to determine the impurities present in it
Apparatus : 1) Test tube

2) Pychnometer

3)pH Meter
Sampling of R.M. : As per sampling plan

i)Weigh accurately empty pychnometer for Density

=tr
ii)Fillthe Pychnometer with water

iii) Note down the weight of pychnometer + Water

= B

iv) Dry the pychnometer and note down Weight of Pychnometer + perfume
sample = C
tror Hindustaa Agfo
Sdoo^
proprictJi-

HINDU5TAN AGR
An ISO 9OO1 = 2O1 5 Gentified Gompany
Haridwar Road Mandawar, District Bijnor; U.P. India"(24672L)
Email : info@hindustanagrotech,com, hindustanagrolT@gmail.com I Web.: www.hindustanagrotech.com
Contact : +91-8859497996, 9720553875
GSTIN : O9CXIPS6919R1Z7 | tEC CODE : CXIPS5919R
r",:.
v)Ensure the level of liquid for both water as weil as perfume
':,ir

Calculation:

c-A
Density = x 0.998
B-A

tror Hindustan Agft

g*e>
ProPricter

HINDU5TAN AGRO
An ISO 9OO1 = 2O1 5 Gertified Gompany
Haridwar Road Mandawa[ District Bijnor, U.P. India (24672t)
Email info@hindustanagrotech.com, hindustanagrolT@gmail,com lWeb,:www.hindustanagrotech.com
Contact : +9 I -8859497996", 972055387 5
GSTIN : O9CXIPS69r9R1z7 | IEC CODE : CXIPS5919R