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淡抹初夏155 002
淡抹初夏155 002
淡抹初夏155 002
1733-1735, 1993
ACCELERATED PAPER
Direct enzymic detection of endogenous oxidative base damage in
human lymphocyte DNA
Andrew R.Collins, Susan J.Duthie and pH 8.0), drained and the agarose covered with 25 ii\ of either
Victoria L.Dobson buffer or endonuclease HI in buffer, sealed with a cover glass
and incubated for 30 min at 37°C. Further steps (alkaline
Rowett Research Institute, Greenbum Road, Bucksburn, Aberdeen
AB2 9SB, UK electrophoresis, neutralization and staining with DAPI) were as
described (7). Cells were examined with a Zeiss Axioskop
(A
60min
(0 75
• + buffer + enzyme
Omin "5
incubation • incubation
no repair 1 h repair
1 50
m 25
s
B 3 4 0 1
Comet class
1734
Oxidative base damage detection
Acknowledgements
This work was supported by the Scottish Office, Agriculture and Fisheries
Department, and the Ministry of Agriculture, Fisheries and Food. We thank Dr
R.Cunningham for the bacterial strain overproducing endonuclease HI.
Received on May 27, 1993; revised on July 19, 1993; accepted on July 20, 1993
1735