MLT217 HISTOTECHNOLOGY

AT-3

Case study 1 Roll no – 1 to 10

A 46 years old man was suffering from infection of eye. On observation eye
specialist suggested to remove the eye and sent for histopathology report to rule out
the cancer.

Q1. How will you receive, record and gross the specimen for histopathological
examination?
Q2. Which fixative you choose to fix the specimen and why?
Q3. Which embedding method you will choose to embed the eye ball and how will
you embed it?

Case study 2 Roll no – 11 to 20

A specimen of whole brain (Autopsy) is received in the lab to know the cause of the
death. The C.S.F findings was sugar – 10 mg% and proteins – 60mg% are
suggestive of meningitis.

Q1. Which fixative you will you choose to fix the specimen and why?
Q2. How will you fix the specimen and suspend it in the fixative?
Q3. If you place it in the container without hanging, what will be the effect of it on the
tissue?

Case study 3 - Roll no – 21 to 30

A 70 years old male complains of pain in passing the stool for the last 2 months. The
complete blood count was normal, stool sample shows many undigested food
particles. Ultrasound shows a mass measuring 4X5cm. Intestine is removed
surgically and sent for routine HPE.

Q1. Which fixative you will choose to fix the specimen and how?
Q2. How will you process the specimen for routine HPE?
Q3. How will you orient the tissue while making a block?
Q4. Which stain you will choose to stain the specimen and why?

Case study 4 Roll no – 31 to 40

A specimen of uterus was processed for routine histopathological examination using
alcohol and xylene. Paraffin blocks are prepared and during section cutting the
tissue was not coming.

Q1. What will be the possible reason of not getting the tissue in sections?
Q2. How will you correct the problem mentioned in case?
Q3. Give protocol you follow to correct the problem.

Case Study – 5 Roll no – 41 to 50

A 46 year old female patient felt a 1.5x2 cm mass over the upper outer quadrant of
her left breast for the last 8 months. It was movable and non-tender. No consultation
was done. She noted gradual enlargement of the same mass but was non-tender.
One month later, she noted skin dimpling on her left breast. Persistence of the above
symptoms with now accompanying tenderness on the left breast prompted her to
seek consult.

Family History: Mother died of breast cancer, father has hypertension and diabetes
mellitus.

Q1. What is your differential diagnosis based on history and physical examination?
Q2. Based on the differential diagnosis, what is the etiology?
Q3. Which of the laboratory tests should be suggested or performed to confirm the
diagnosis?