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Tutin. 1940. The Algae.
Tutin. 1940. The Algae.
THE ALGAE
BY T. G. TUTIN,M.A., Assistant Lecturer in Botany, Victoria University, Manchester
(Plate VI. Three text-figures)
Read 9 May 1940
CONTENTS
PAGE
I. Chemical factors . . 191
11. Phytoplankton . . 192
111. Algae of ponds . . 195
IV. Systematic list of algae . . 196
V. Summary . . 202
VI. References . . 202
I. CHEMICAL FACTORS
FORthe algal flora the concentrations of dissolved salts, particularly nitrates and phos-
phates, are of great importance, but unfortunately there is not much information about
these in the case of Titicaca. The concentrations of chlorides in the various lakes, which
may be taken as a general indication of the relative salt-contents, have already been given
(Tutin 1940, p. 180) and it will be seen from this table that, except in the case of Lagunilla
Saracocha and Lake Langui, this is very high for fresh water. The estimation of nitrates
in Titicaca was difficult owing to this high concentration of other salts, but phosphates
were present in about the quantities usually found in fairly rich temperate lakes. Silica,
which is of importance t o diatoms, was always abundant. The concentration of calcium
was high enough not to limit algal growth, as will be seen from Table I.
TABLE I
LAKE Ca (mg./l.)
Lagunilla Lagunilla 27.0
Lago Pequeiio 63.4
Lago Grande 68-8
Lagunilla Saracocha 95-0
It is interesting to note that calcium is much more abundant in Lagunilla Saracocha than
in Lagunilla Lagunilla, though the former has only 68 p.p.m. C1, while the latter has 236.
It will also be seen that calcium is somewhat lower in the Lago Pequeiio than in the Lago
Grande, while the reverse is true of the chlorides. The most probable explanation of this
seems to be that the lime-incrusted Charophyta, which occupy a large area in the Lago
Pequeiio but are comparatively scarce in the Lago Grande, produce this difference by
removing calcium in appreciable quantities from the relatively small volume of water in
the Lago Pequefio.
Since our visit to Lake Titicaca was confined to the winter, during which the climate
shows little variation, large changes in the plankton or in the dissolved salts were not to
be expected, and such changes did not in fact occur.
24-2
192 PERCY SLADEN TRUST EXPEDITION
11. PHYTOPLANKTON
Plankton collections were made at intervals throughout our stay in widely separated
parts of the lake with the ordinary silk tow-net of 71 meshes per cm., which was towed
slowly through the water near the surface. These were supplemented by collections made
with the Harvey net (Harvey 1934), which was hauled vertically for 1 0 m . and then
closed, so that collections could be made over a definite depth range to discover if any
horizontal stratification of the plankton existed. The volume of water filtered through
this net in each haul was recorded and so an estimate of the density of the population
could be made by counting the numbers of organisms in a known proportion of the catch.
Although Titicaca has a high salt-content the plankton is not of the kind usually
associated with such waters. The thirty collections made in different parts of the lake
down t o a depth of 50 m. show an unusual degree of uniformity, in marked contrast to the
variations from place t o place generally found even in much smaller bodies of water. It
will be noticed that in this respect the plankton resembles the rooted vegetation, and it is
possible that the Uniformity in this case also may be due to the rather limited number of
species which occur.
The following 15 species were found in the plankton of the Lago Grande, the first five
being present in all, or nearly all, of the collections:
impossible to draw any definite conclusions about this matter; indeed the evidence is
mainly negative. The average numbers per litre for all the organisms (excluding Botryo-
coccus), and for Dictyosphaerium separately, are also given in Table I1 from which it
will be seen that the plankton is always sparse compared with that found in Lake
Mendota, for example, where filaments of the diatom Melosira alone may reach a figure
of nearly 30,000 per litre (Birge and Juday 1922). No collections were made of the
nannoplankton, which in many lakes has a density of several million individuals per
litre.
TABLE I1
Depth in motros : ... ... 0-10 10-20 20-30 30-40 40-50 Date
Dictyosphaerium EhrerLbergiawum 1930 840 1370 1350 1130 5 July
1070 1300 1470 1290 - 19 J u n e
- - 1960 1300 1950 22 July
Staurastrum paradoxurn 170 130 190 230 120 6 July
140 80 100 80 - 19 Julie
- - 270 300 390 22 July
Ankistrodesmus longissimus 170 120 170 100 60 5 July
180 190 170 90 - 19 J u n e
- - 50 70 100 22 J u l y
Peridinium sp. 260 300 140 190 160 5 July
60 130 50 80 - 19 J u n e
90 110 140 22 July
Ulothrix szi bt ilissima 90 90 100 60 40 5 July
60 60 60 80 - 19 June
110 130 150 22 July
AVERAGE TOTALS
In the single collection from the Lago Pequefio all the commonest species found in the
Lago Grande occurred, with the rather surprising exception of Dictyosphaerium. The
complete list of this collection comprises seven species, among which only the Oedogonium
was not found in the Lago Grande:
Ankistrodesmus longissimus (Lemmerm.) Wille. Mougeotia sp.
Botryococcus Braunii Kutz. Oedogonium sp.
Staurastrum paradoxurn Meyen. Peridinium sp.
Ulothrix subtilissima Rabenh.
I n the other lakes visited no quantitative collections were made and, with the
exception of Lake Poop6 where two collections were taken, only one collection was made
in each lake. The results of these rather scanty collections are however of sufficient
interest to be listed in full as they indicate a great difference in the composition of the
phytoplankton in the different lakes, in marked contrast t o the uniformity within Titicaca
itself.
LAKE POOP6
Botryococcus B r a m i i Kutz. Rliabdoderma salina Tutin.
Glenodinium sp. Lyngbya aestuarii (Mert.) Liebmann.
In Titicaca with a slightly higher salinity than Lagunilla Lagunilla the average number of
species in a collection is 8 to 9. Lake Poop6 is excluded from this comparison because its
salinity is far in excess of that of normal fresh waters. With the exception of Lake Titicaca
no figures for the density of the population were obtained, but since the collections from
the other lakes showed no obviously great increase in the abundance of the organisms it
may be assumed that they also have rather sparse populations. Since all the collections
were made a t one season in a single year any generalizations made about them must be
regarded with caution.
The distribution of the various types of algae in relation to the concentration of
dissolved salts in these Andean lakes differs considerably from that found by Pearsall
(1921) to occur in the English Lakes. He found that as the amounts of dissolved salts,
silt and macrophytic vegetation increased the plankton was dominated by.the following
algae in turn : Desmids with Sphaerocystis Schroeteri; Desmids and Diatoms (especially
Ttcbellaria); Diatoms with Eudorina and Ceratium ; and finally Asterionella and Cyano-
phyceae. The Andean lakes are found among much softer rocks than occur in the English
Lake District and consequently all show a considerable amount of silting. The
macrophytic vegetation does not vary much in composition or quantity from one to
another, but the amounts of dissolved salts, which are generally thought to be of prime
importance to algae, show a large variation. The sequence of plankton algae, from the
TUTIN-ALGAE 195
lowest to the highest salt concentrations, in these lakes is as follows: Eudorinu; Sphaero-
cystis Schroeteri and Peyidiniurn ; Coelustrurn, Cyanophyceae and a Desmid ; Chloro-
coccales, Botryococcus, Sphaerocystis, a Desmid and, though rarely, a centric Diatom. The
most striking features here are the nearly complete absence of Diatoms, which are usually
dominant in lakes rich in salts, and the fact that the Desmid only occurs in the richer
lakes. The abundance of Cyanophyceae in Lagunilla Lagunilla and their complete
absence from Titicaca is also very remarkable.
-4theory to account for this unusual distribution does not seem possible as the avail-
able data are too scanty and a longer period of observation would be necessary to
establish fully the character and seasonal variation of the plankton of these lakes. The
facts indicate that the correlation between the concentration of mineral salts and the type
of plankton does not hold in all cases and suggest that the investigation of other factors
would be desirable. Harvey (1939) has shown that certain other substances are necessary
for the growth of one species of marine plankton diatom, and these might be found to
play an important part in the life of freshwater plankton algae. The information available,
though inadequate as the basis for a definite statement, may be taken as indicating that
the correlation between the type of plankton and the usually investigated dissolved
substances is, a t least in part, fortuitous.*
Pandorina morum (Mull.) Bory. A224, A225 Capachica stream. Small colonies c. 30p in diameter.
A214 pond near Chapa Hacienda, Capachica peninsula. Obtained on wetting dried mud from
ponds.
Gonium pectorale Mull. Obtained on wetting dried mud from ponds.
G . sociale (Duj.) Warm. Obtained on wetting dried mud from shore of Pun0 Bay. Very abundant.
Eudorina elegam Ehrenb. P.F.H. 215 Lake Langui. Sole constituent of the plankton.
Volvox aureus Ehrenb.* A240 stream in Choccocoya Bay; A260 pond, Capachica peninsula.
TETRASPORINEAE.
Gloeocystis ampla Kutz. Dried mud collected near high water mark in Puno Bay.
Palmodictyon varium (Naeg.) Lemmerm. A200, A205 ponds, Capachica peninsula.
Sphaerocystis Schroeteri Chodat. Spring near Lagunilla Lagunilla, alt. 4300 m. ; P.F.H. 232 plankton,
Lagunilla Saracocha; A200, A202, A 2 1 1 ponds, Capachica peninsula; dried mud from Capachica
stream and ponds ; P.F.H. 84, A216, A229, A233, A236, A243, A244 plankton, Lake Titicaca.
Sporadic.
2. CHLOROCOCCALES
Tetraedron trigonum (Naeg.) Hansg. Obtained on wetting mud from shore of Pun0 Bay.
Radiococcucl sp. Obtained on wetting mud from a pond, Capachica peninsula. Colonies of four cells,
more loosely aggregated in groups of four in a mucilage envelope c. 5 p br. with distinct radial
striations. Cells 7 p 1.) 4 p br., chloroplast shallow cup-shaped, pyrenoid solitary. The Peruvian
specimens differ from the two species of this genus in having the cells distinctly elongated, not
round, and the mucilage envelope rather narrow, but nevertheless seem to belong here as the
structure of the chloroplast and mucilage envelope is that characteristic of the genus.
Oocystis gigas Archer var. Borgei Lemmerm. P.F.H. 284, A234, A236, A237, A239, A246-250, A266,
A268 plankton, Lake Titicaca. Commoner in July than earlier.
Lagerheimia sp. A238, A247, A249, A266 plankton, Lake Titicaca. Collected with the Harvey net
between 10 and 30 m. ; always rare.
Actinastrum Hantzschii Lagerh. Obtained on wetting mud from a pond, Capachica peninsula.
Ankistrodesmucl longissirnus (Lemmerm.) Wille. P.F.H. 225 plankton, Lagunilla Lagunilla ; P.F.H.
164 plankton, Lake Titicaca (Lago Pequefio); P.F.H. 84, P.F.H. 284, A216, A219, A227, A229,
A232-234, A236-239, A243, A244,8246-251, A254, A255, A266-268 plankton, Lake Titicaca.
Occurred in every plankton collection made in this lake.
A. falcatucl (Corda) Ralfs. Obtained on wetting mud from the shore of Puno Bay. In groups of 2-10
individuals, 2 p br.
Dictyosphaerium Ehrenbergianum Naeg. In all plankton collections from Lake Titicaca as above.
Absent from Lagunilla Lagunilla and Lago Pequeiio. A 235 Capachica stream ;obtained on wetting
mud from the shore of Pun0 Bay.
Pediastrum bidentulatum Al. Braun. A 205 pond, Capachica peninsula.
P. Boryanum (Turpin) Menegh. A233, A248, A266, A267 plankton, Lake Titicaca. Occurring down
to 4 0 m . , but not common. Obtained on wetting mud from Capachica stream and shore of
Pun0 Bay.
P. Boryanum (Turpin) Menegh. var. granulatum Kutz. Obtained on wetting mud from ponds,
Capachica peninsula.
P. cf. Kawraiskyi Schmidle. A216 plankton, Lake Titicaca. Marginal cells of coenobium c. 7p 1. and
6 p br. with two processes 2 p 1. lying over each other. Inner cells more or less rectangular, c. 6 p
diam. with triangular spaces between them with sides 3 - 3 . 5 ~1. Cell walls smooth, processes
smooth and blunt. P.F.H. 84, A266 plankton, Lake Titicaca. Similar to A216 but without
perforations in the coenobium.
Pediastrum sp. A241 small lake in crater, San Antonio de Esquilache. Coenobium c. 7 5 p diam., outer
cells nearly square 9 p diam., with a rather broad indentation 4-5p deep, and two processes 6 p 1.
Inner cells c. l o p br., 4-5p 1. with a broad, shallow rather asymmetrical indentation on their
outer side.
* I have to thank Dr M. A. Pocock for identifying the specimans of Volvox.
TUTIN-ALGAE 197
these again very loosely united in groups of four, the large colonies reaching 4 2 p 1.
and 2 7 p br.
Scenedesmus denticulatus Lagerheim. A 225, A 226 Capachica stream.
S. incrassatulus Bohlin. Obtained on wetting mud from Capachica stream.
S. obliquus (Turpin) Kutz. A225, A235 Capachica stream; obtained on wetting mud from Puno Bay.
S. quadricauda (Turpin) BrQb. Obtained on wetting mud from Laguna Tejane (Titicaca) and from
shore of Pun0 Ray.
Coelastrum microporum Naeg. P.F.H. 225 plankton, Lagunilla Lagunilla, abundant; A254, A237,
A248, A249, A268 plankton, Lake Titicaca, rare; collections, except the first two, made with the
Harvey net between 10 and 50 m.
Cliaracium falcatum Schroeder. Obtained on wetting mud from shore of Puno Bay.
C. Sieboldii A. Braun. A226 Capachica stream, common; obtained on wetting mud from poad,
Capachica peninsula.
3. ULOTHRICALES
Ulotluix swhtilissima Rabenli. P.F.H. 164 plankton, Lake Titicaca (Lago Pequeiio). All plankton
collections from Lake Titicaca (Lago Grande) except P.F.H. 284. (See under Ankistrodesinus
longissimus.)
U . variabilis Kutz. Obtained on wetting mud from pond, Capachica peninsula.
Monostroma sp. A228 fast-flowing stream near Puno.
Sphaeroplea annulina (Roth.) C. A. Agardh. Obtained on wetting mud from Capachica stream.
4. CLADOPHORALES
Cladopliora glomeratn (I,.) Kiitz. ~ i ~ i pBrand.
l. A 222-224 Capachica stream, on stones; A257, A 25!).
A261 Taman, common on boulders; A256 Rio Urubamba a t Quillobamba (1100 m.); A207
? young plants, Taman.
C. crispata (Roth.) Kutz. ampl. Brand. A258 Taman, on boulders; in deeper water than the last.
C. cf. fracta Kiitz. ampl. Brand. A 256 Rio Urubamba at Quillobamba.
5. CHAETOPHORALES
Stigeoclonium sp.A. A220, A 221 Capachica stream. Little branched, branches short and thorn-like,
occasionally long, alternate. Cells 15p br., about three times as long, chloroplasts small. Forming
zoospores. Also obtained on wetting mud.
Stigeoclonium sp.B. Obtained on wetting mud from ponds, Capachica peninsula. Creeping system
extensive, cells rather variable in size, usually rounded, distal ones elongated, 2-3 times as long
as broad. Chloroplasts large. Erect shoots often arising in groups of 2 or 3, cells of main axis
c. 9 p br., 15-3Op l., constricted at end walls. Chloroplast a parietal band round the middle of
the cell. Branches mostly alternate, ending in a very long hyaline hair. Cells from which branches
arise 10-12p l., 9 - l o p br. Cells of branches c. 2 5 p l., 6 p br. Branches usually about 150p 1. with
hairs about 8OOp 1. I n some plants the constrictions between the cells of the main filament were
TRANS. LINK. S O L ( 3 ) , VOL. I, I?. 2. 25
198 PERCY SLADEN TRUST EXPEDITION
very marked, and the branching dichotomous, the cells from which the branches arose being
nearly round and about 8 p in diameter.
The taxonomy of Stigeoclonium is in a confused state and no specific determination of the
above forms seems possible a t present.
Aphanochaete plychaete (Hansg.) Fritsch. A 2 12 pond, Capachica peninsula.
A . repens A. Braun. Obtained on wetting mud from Capachica stream.
Gliaetopeltis orbicularis Berthold. A262 common on microscope slides left for three weeks in 0.5 m.
on Zannichellia in moderate shelter, in 1.5 ni. among totora and in 0-5 m. on a bare sandy bottom
in Taman.
6. OEDOGONIALES
Bulbocliaete sp. Obtained on wetting mud from a pond, Capachica peninsula. Sterile.
Oedocladium sp. A 220 Capachica stream. Sterile.
Oedogonium andinum sp.nov. (Fig. 2). Obtained on wetting mud from the shore of Puno Bay and from
a pond, Capachica peninsula.
Nannandrum, gynandrosporum. Filamentum 10-15p dianietro,
cellulis 130-14Op longis; cellula basalis plus minusve inflata. An-
drosporangia 12p lata, 16p longa. Nannandria stipite 27 p longo,
9 p lato, piriforme ; antheridium unicellulatum 9 p longum, 9 p
latum. Oogonia solitaria, 28-42 pdiametro, 40-55 p longa, poro lato
superiore. Oospora matura castanea, levis, oblonga vel sphaerica,
oogonium totum apice porum versus except0 complens, 30-46p
longa, 25-4Op lata.
Nanandrous, gynandrosporous. Diam. of filament 10-1 5 p,
cells 130-140p I., basal cell usudly somewhat swollen. Andro-
sporangia 12p br., 16p 1. Stalk cell of dwarf males 2 7 p l., 9 p br.,
piriform; antheridium 1-celled, 9 p l., 9 p br. Oogonia solitary, 28-
4 2 p diam., 40-55p l., opening by a wide superior pore; ripe oospore
chestnut brown, smooth, oblong or spherical, completely filling the
oogonium except for the top near the pore, 30-46p l., 25-4Op br.
Differs from 0. Hoersliolmiense Hallas, which it most nearly
resembles, in the nearly spherical oospore, the solitary antheridium
and the smaller dwarf male.
I
0. armigerum Hirn. Obtained on wetting mud from a pond, Capachica
peninsula.
0. cf. cymatosporum Wittr. & Nordst. Obtained on wetting mud from ~ i 2. ~Oedogoniuln .
the shore of Pun0 Bay, and from Capachica stream. Diam. of sp.nov. ( x 395).
filament 7-1 1 p, vegetative cells often short, with constrictions
between them. Diam. of oogonium 18-33p, subspherical or depressed spherical, single or in pairs,
pore median. Mesospore finely scrobiculate. The specimens differ from the description of
0. cymatosporum in having constrictions between the cells and in the small size of the oogonium.
0. Franklinianum Wittr. Obtained on wetting mud from the shore of Pun0 Bay.
0. globosum Nordst. A204, A205, A21 1 ponds, Capachica peninsula.
0. Howardi G. S. West. Obtained on wetting mud from ponds, Capachica peninsula. Filament 10-1 1 p
br., cells 3-4 times as long as broad, distinctly capitellate. Oogonia depressed globose with a
distinct median split, c. 30p diam., oospore completely filling the oogonium.
0.inconspicuum Hirn. A211, A213 ponds, Capachica peninsula; also obtained on wetting mud from
these ponds and from the shore of Pun0 Bay.
0. Landsboroughi (Hass.) Wittr. A205 pond, A213 ditch near Hacienda Chapa, Capachica peninsula.
0. lageniforme Hirn. A 205 pond, Capachica peninsula.
0. mammiferum Wittr. Obtained on wetting mud from shore of Pun0 Bay.
0. mitratum Hirn. A212 shallow drying pond, Capachica peninsula.
0. cf. multisporum H. C. Wood. A211 pond, Capachica peninsula. The specimens agree with this
species in having very short vegetative cells, oogonia in groups of 2-3 and in the dimensions, but
differ in having a median pore instead of a superior one.
TUTIN-ALGAE 199
Oedogonium pisanum Wittr. Obtained on wetting mud from the shore of Pun0 Bay.
0. plagiostomum Wittr. A205, A213, A215 pond and ditch, Capachica peninsula. Some of the
specimens appear to be referable to 0. gracilius (Wittr.) Tiff. (0.plagiostomum var. gracilius
Wittr.), but since the only differences between these two species mentioned by Tiffany (1937,
p. 36) are in the dimensions of the cells, and there appears to be every intermediate, I have
included all my material under the one name which is therefore used in a broad sense.
0. cf. psaegmatosporum Nordst. Obtained on wetting mud from the shore of Pun0 Bay. The
Peruvian specimens appear to be dioecious but otherwise agree well with the description of this
species.
0. cf. rugulosunt Nordst. Obtained on wetting mud from the shore of Pun0 Bay. No dwarf males seen.
0. sociale Wittr. A 205 pond, Capachica peninsula.
0. suecicum Wittr. A213 ditch, Hacienda Chapa, Capachica peninsula; also obtained on wetting mud
from Capachica stream.
0. varians Wittr. & Lundell. A203 pond, Capachica peninsula.
0. cf. Zehneri (Tiff.) Tiff. Obtained on wetting mud from pond, Capachica peninsula.
Oedogonium sp. A21 1 pond, Capachica peninsula. Nanandrous. Vegetative cells 6 - l o p br., 14-2Qp 1.
Dwarf male usually on oogonium, stalk cell 7 p br., lop l., antheridium 6 x 6p. Oogonia in groups
of 2-3, depressed globose, c. 20p diam., pore median, wall of oospore smooth. Most nearly re-
sembles 0. depessum Pringsh. from which it differs in the shorter, narrower vegetative cells, the
somewhat larger dwarf male and in having the oogonia in groups of 2-3.
Oedogonium sp. P.F.H. 164 plankton, Lake Titicaca (Lago Pequefio). Cells c. Gp br., 30-35p l.,
chloroplasts small. Cell division occurring freely. Sterile.
Sterile specimens of Oedogonium were also found in collections from Capachica stream and on
microscope slides left for three weeks in 0.5 m. on Zannichellia in moderate shelter, in 1.5 m.
among totora and in 0.5 m. on a bare sandy bottom in Taman.
7. CONJUGALES
Zygnema peliosporum Wittr. A 201 small pond, Capachica peninsula. Sterile specimens of Zygne?na
were also collected from several other ponds and the Capachica stream.
Mougeotia viridis (Kutz.) Wittr. Obtained on wetting mud from a pond, Capachica peninsula. This
species produced zygospores freely under laboratory conditions.
Mougeotia sp. P.F.H. 164 plankton, Lake Titicaca (Lago Pequefio); P.F.H. 84, A216, A233, A234,
A 247, A266, A268 plankton, Lake Titioaca (Lago Grande). Small sterile filaments occurring
sparsely at depths down to 50 m. Cells 5-6p br.
Sterile specimens of other species of Mougeotia were collected from a small lake a t San Antonio
de Esquilache (alt. 4700 m.) and on microscope slides left for three weeks in 0.5-1.5 m. in Taman.
Filaments were also obtained on wetting mud from a pond, Capachica peninsula.
Spirogyra cataeniformis (Hass.) Kiitz. Obtained on wetting mud from a pond, Capachica peninsula.
S. injlata (Vauch.) Rabenh. A203-205, A211, A212, A215, A261a ponds, Capachica peninsula.
S. Jiirgensii Kiitz. A2Q0-202, A204, A205, A212, A213, A261a ponds and ditches, Capachica penin-
sula. There is a slight doubt whether these specimens should be referred to this species or to
S. decimina, but on the whole they seem to agree best with S . Jiirgensii.
S. longata (Vauch.) Czurda. A 205, A 2 1 I , A 212, A 2 15 ponds, Capachica peninsula. Rather a narrow
form.
S. cf. porticalis (Mull.) Cleve. A21 1 pond, Capachica peninsula.
S. sticticu Wille (Sirogonium sticticum Kiitz.). A200, A202-205, A213, A215 ponds and ditches,
Capachica peninsula; also obtained on wetting mud from ponds and Capachica stream; A209
pool behind shingle bank, Taman (sterile).
S. varians (Hass.) Kutz. A205, A211 pond, Capachica peninsula; A225 Capachica stream.
S. Weberi Kutz. A204 pond, Capachica peninsula.
Sterile specimens of Spirogyra were common in most of the collections given above, and also
in collections from a well among Inca ruins, Titicaca Island, a small lake near Lagunillas a t
4400 m., and a spring near Lagunilla Lagunilla a t 4300 m.
25-2
200 PERCY SLADEN TRUST EXPEDITION
Closterium acerosum (Schrank) Ehrenb. Obtained on wetting mud from Laguna Tejane (Titicaca).
Produced zygospores freely under laboratory conditions.
C . Cynthia De Not. Obtained on wetting mud from a pond, Capachica peninsula, and from Laguna
Tej ane.
C . cf. Leibleinii Kutz. Obtained on wetting mud from a pond, Capachica peninsula.
Euastrum sp. Obtained on wetting mud from a pond, Capachica peninsula.
Staurastrum paradoxum Meyen. P.F.H. 225 plankton, Lagunilla Lagunilla.
8. SIPHONALES
Vaucheria hnmata Walz. A202, A205 ponds, Capachica peninsula; also obtained on wetting mud from
a pond.
Vaucheria sp. Spring near Lagunilla Lagunilla, 4300 m. and stream near Urmiri, Pazfia, Bolivia.
9. XANTHOPHYCEAE
Botryococcus Rrnunii Kiitz. P.F.H. 225 plankton, Lagunilla Lagunilla; uncommon ; P.F.H. 232
plankton, Lagunilla Saracocha; P.F.H. 192, 193 plankton, Lake Poop6; P.F.H. 164 plankton,
Lake Titicaca (Lago Pequefio); P.F.H. 84, P.F.H. 284, P.F.H. 287, A210, A219, A227, A229,
A232, A234, A236, A243, A244, A251, A254 plankton, Lake Titiraca (Lago Grande). Usually
very abundant.
Z'ribonema sp. Obtained on wetting mud from Capachica stream.
10. DINOPHYCEAE
Nodularia Harveyana Thur. A216, A219, A232, A234, A238, A239, A246, A250, A266, A267
plankton, Lake Titicaca (Lago Grande) ; fairly frequent but never abundant down to 50 m. ; also
obtained on wetting mud from the shore of Pun0 Bay.
Nostoc Linkia (Roth.) Bornet. A204 pond, Capachica peninsula; A220 Capachica stream, near the
mouth; A258 Taman, on boulders in about 0.5 m.
N . piscinale Kiitz. A210 pond, Capachica peninsula.
N . sphaericum Vauch. A208, A258 Taman, on sides of boulders; A217 Taman, on moss (Sciaromium)
down to about 14 m.; A210 pond, Capachica peninsula; also obtained on wetting mud from this
pond. Common on boulders, as an epiphyte on Potamogeton and other plants in sheltered places,
and on Sciaromium a t the lower limit of vegetation.
Anabaena catenula (Kutz.) Bornet & Flah. A263 stream running into Urufii Bay; in pools among
rocks.
A . cylindrica Lemmerm. A220 Capachica stream, near the mouth. Another unidentifiable species of
Anabaena was also collected in this stream.
Anabaenopsis peruviana sp.nov. Obtained on wetting mud from a pond, Capachica peninsula, and from
Laguna Tejane, Lake Titicaca.
Trichomata spiralia plerumque cum anfractis duobus, nonnulla inter se convoluta vaginis
nullis. Cellulae cylindricae, septis distincte constrictae, pseudovacuolis nullis, 4-5 p latae,
6-7 p longae. Heterocystae ad terminos utrosque filamentorum positae, nunquam intercalares,
ellipsoideae, 6 p latae, 9-1011 longae.
Trichomes spiral, mostly with two turns, several coiled together, sheaths absent. Cells
cylindrical, distinctly constricted a t the cross walls, without pseudo-vacuoles, 4-5p br., 6-7 p 1.
Heterocysts a t both ends of the filaments, never intercalary, ellipsoidal, Gp br., 9 - l o p 1. This
species appears to resemble A . Tanganyikae (G. S . West) Wolosz. most nearly but differs from it in
being larger ( A . Tanganyikae: cells 2 - 6 2 . 6 p br., 3 . 8 - 8 . 5 ~l., heterocysts 3 x 5 . 5 ~ having
) ~ the
cells shorter in proportion to their breadth, and distinctly constricted a t the cross walls.
Cylindrospermum Titicacae sp.nov. (Fig. 3). Obtained on wetting mud from the shore of Pun0 Bay.
Trichomata solitaria, recta, brevia, natantia. Cellulae septis
valde constrictae, 3.0-3.5 p latae, sesquilongae usque ad duplo
longiores quam latae. Heterocystae ellipsoideae, sporam versus in
projcctionem brevem obtusam constrictae, 4 p latae, 6 p longae.
Sporae solitariae, leves, contcntis granulatis, cylindricae, c. 5 p
latae, 10-12 p longae. Sporae veteriores, a trichomate segregatae,
ala mucilaginosa quam spora semilata instructae.
Trichomes solitary, straight, short, free-floating. Cells strongly
constricted a t the cross walls, 3 . 0 - 3 . 5 ~br., once and a half to twice Fig. 3. Cylindrospermum,
as long as broad. Heterocysts ellipsoidal with a short blunt point Titicacae sp.nov.
at the attachment to the spore, 4 p br., Gp 1. Spores solitary, wall
A, filament; R, detached spore.
smooth, contents granular, cylindrical, c. 5 p br., 10-12p 1. Older
spores, detached from the trichome, develop a wing of mucilage about half the width of the
spore. Differs from C. minimum G. S. West, described from the Central Andes (alt. 2300 m.), in
the larger size of the cells and the marked constrictions between them.
Spirulina Jenneri (Stizenb.) Geitl. A214 pond, Capachica peninsula.
S. laxa G. M. Smith. Obtained on wetting mud from Chapa pond, Capachica peninsula.
S. laxissima G. S . West. A222 Capachica stream.
S. subtilissima Kutz. A 2 2 1 Capachica stream.
An unidentified species of Spirulina was also obtained on wetting mud from the shore of
Pun0 Bay.
Oscillatoria ornata Kiitz. Obtained on wetting mud from a pond, Capachica peninsula.
A species of Oscillatoria was also found in a plankton collection from Lagunilla Lagunilla
(P.F.H. 225).
Lyngbya aestuarii (Mert.) Liebmann. P.F.H. 192, P.F.H. 193 plankton, Lake Poop6, in brackish
water.
L. Martensiana (Kutz.) Gom. A242 small lake in crater, San Antonio de Esquilache (alt. 4700 m.).
202 PERCY SLADEN TRUST EXPEDITION
V. SUMMARY
The lakes investigated show a great variation in the concentration of dissolved salts,
the chloride content ranging from 37 to 5000 parts per million. Nutrient salts are
present in Titicaca in about the amounts usually found in fairly rich temperate lakes, and
silica is always abundant.
The phytoplankton of Titicaca is of the type usually associated with waters poor in
nutrient salts. Contrary t o what has been found in most lakes, the number of species in
the plankton of the Andean lakes examined (with the exception of Lake Poop6) decreases
with decrease in the concentration of dissolved salts. One species of Desmid occurs in the
plankton and that is found only in the lakes with a high concentration of dissolved salts.
It is suggested that something other than nitrate and phosphate may be the factor
controlling the distribution of these plants.
The density of the plankton population in Lake Titicaca is very low compared with
North American lakes.
A list of all the algae collected in an identifiable condition is given and this includes
descriptions of seven species which do not appear to have been previously described.
VI. REFERENCES
BIRGE,E. A. and JUDAY,C. 1922. “The inland lakes of Wisconsin. The plankton: I. Its quantity and
chemical composition.” Wisconsin Geol. Nat. Hist. Surv. Bull. no. 64.
HARVEY, H. W. 1934. ‘(Measurement of phytoplankton population.’’ J . Mar. Biol. Ass. XIX, 761.
__ 1939. ‘(Substances controlling the growth of a diatom.” J . Mar. Biol. Ass. XXIII, 499.
PEARSALL, W. H. 1921. “The development of vegetation in the English Lakes.” Proc. Roy.SOC.B,
XCII, 259.
TIFFANY,L. H. 1037. “Oedogoniales” in North American Flora, XI, pt. 1. (New York Botanical
Garden.)