886 Column oven

Service Manual

0060.070-16 January 2021

Original instructions

Copyright Spark Holland © 2021 All rights reserved. No part of this publication may be reproduced, stored in a retrieval system
or transmitted in any form by any means, without written permission from the manufacturer.
The manufacturer reserves the right to make changes to the design and specifications of the system and this manual without
prior notice.

2
Contents

1 About this manual 5

1.1 Pictorials 5
1.2 Safety instructions 6

2 Identification of parts 10
2.1 Instrument - front 10
2.2 Instrument - rear 11
2.3 Supply line and preheater 11
2.4 Options 13

3 Control software 14
3.1 Service Menu 14

4 Maintenance 20
4.1 Column selection valve - rotor seal replacement 20
4.2 Diverter valve - rotor seal replacement 21
4.3 Diverter valve - valve replacement 22
4.4 Replacing fuses 22

5 Trouble shooting 23

6 Disassembly 23
6.1 Removing the left side panel 23
6.2 Inside the column oven 25
6.3 Heater fan motor replacement 25
6.4 Peltier cooling assembly replacement 29
6.5 Main PCB replacement 31
6.6 Sensor PCB replacement 34
6.7 Column selection valve assembly replacement 36
6.8 Diverter valve assembly replacement 37
6.9 Front panel display ASSY replacement 40

7 Performance inspection 41
7.1 SOP check 41
7.2 Temperature calibration check 41

Appendix A. Spares and accessories 43

Appendix B. Error codes 45
Appendix C. Storage, packaging, recycling and disposal 47
Appendix D. PCB Layouts 48

Appendix E. Exploded views 50

Appendix D. Programming overview 64

4
1 About this manual

This service manual is written for trained and qualified service engineers who
perform service procedures on the column oven.

1.1 Pictorials

The following pictorials are used in this manual:

This sign warns about the risk of electrical shock. It calls
attention to a procedure or practice which, if not adhered to,
could result in electrocution.

Do not proceed beyond this danger sign until the indicated

conditions are fully understood and met.

The warning sign denotes a hazard. It calls attention to a

procedure or practice which, if not adhered to, could result in
severe injury, loss of life or damage or destruction of parts or all
of the equipment.
Do not proceed beyond a warning sign until the indicated
conditions are fully understood and met.

The caution sign denotes a hazard. It calls attention to a

procedure or practice which, if not adhered to, could result in
damage or destruction of parts or all of the equipment.
Do not proceed beyond a cautions sign until the indicated
conditions are fully understood and met.

The fire/flammable sign draws attention to the fact that use of

flammable solvents or samples may cause a significant safety
risk.

The toxic hazard sign draws attention to the fact that use of toxic
solvents or samples may carry a significant health risk.

The biohazard sign draws attention to the fact that use of

biological materials, viral samples and needles may carry a
significant health risk.

The hot surface sign calls attention to parts in the instrument that
must not be touched, as they may cause burns

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 The optical radiation sign warns about possible eye injury due to
hazardous optical radiation.

The attention sign signals relevant information. Read this

information, as it might be helpful.

The note sign signals additional information, providing advice or

a suggestion that may support you in using the equipment.

This symbol indicates that the waste of electrical and electronic

equipment must not be disposed as unsorted municipal waste
and must be collected separately. Please contact an authorized
representative of the manufacturer for information concerning
the decommissioning of equipment.

1.2 Safety instructions

Adhere to standard quality control procedures and the following equipment

guidelines when using the system:
General safety
The system working environment should meet the specifications
indicated in this manual.

The system is intended to be used in a regulated laboratory

environment with trained operators following good laboratory
practices.

Use this system ONLY for its intended use. Use of the system
for any other purpose may cause unsafe situations.

Do not use the system if there is any visible damage.

The system working environment should meet the specifications

indicated in this manual.

System operation and maintenance

To keep up the specified performance of the system,
maintenance must be carried out as indicated in this manual.

Service contracts and preventive maintenance services are

available. Please contact your local dealer or the nearest sales
office for more information.

6
Electrical safety
The system must be connected to a suitable mains power supply
with a correctly installed protective earth conductor. Never use
the system without a properly connected protective earth
conductor.

Read the installation instructions before connecting the system

to the mains power supply.

Removal of panels may expose users to lethal voltages. For that

reason, this should only be done by qualified service engineers.

Disconnect the system from the all power sources before

removing protective panels.

Replace or repair faulty insulation on power cords immediately.

Solvents
The solvents used may be flammable.
The room in which the system is installed should be well
ventilated to prevent that solvent vapors cause a fire.

Do not allow solvents to accumulate in the system.

Perform periodic leak checks on solvent lines.

Solvent handling
When handling solvents always observe good laboratory
practices. Know the properties of the solvents used. This
information can be found in Material Safety Data Sheets (MSDS)
supplied by the vendor.

Avoid the use of the following solvents when using a steel

containing flow path:

• Solvents containing strong complexing agents like EDTA.

• Strongly acidic mobile phases (pH <1).

• Halogenated solvents or additives that form radicals

and/or acids.

• Solvents able to form peroxides like chromatographic

grade ether and non-stabilized THF, dioxane and
diisopropyl ether. In case use of these solvents is
unavoidable, they need to be filtered through dried
aluminum oxide in order to remove the peroxides.

Toxic Hazard
When you use or analyze toxic solvents you need to take all
possible precautions and treat all specimens as potentially toxic.

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Leaking solvent connections can cause vapor accumulation in
the oven compartment. These vapors can be toxic. Make sure
you do not breathe in vapors while opening the oven door.
Always wear protective goggles and gloves when handling toxic
samples.

Biological Hazard
When you analyze biological solvents, you need to take all
possible precautions and treat all specimens as potentially
infectious.

Always wear protective goggles and gloves when handling toxic

or biologically infectious samples.

Waste disposal
Dispose of waste (samples, solvents, device) in accordance with
a regulated waste disposal program.
Follow a regulated, approved waste disposal program and never
dispose of solvents through the municipal sewage system.

Temperatures inside the column oven compartment may be

high.
Touching the panels inside the compartment may cause burns.
Make sure the actual temperature inside the compartment has
decreased to a value of e.g. 25°C before you open the oven
compartment to prevent injuries.

Applications: quality control

It is recommended that you routinely run several quality control
samples.

Quality control samples should represent low, average and high

levels of a compound. Make sure that quality control sample
results are within an acceptable range, and evaluate precision
from day to day and run to run.

Data collected when quality control samples are out of range

may not be valid. Do not report this data until you are certain that
system performance is acceptable.
Apart from use of quality control samples, we recommend that
you use blanks. The blanks will help you assess whether carry-
over is within an acceptable range, and they will help you in
monitoring the integrity of your data.

It is also recommended that you use a proper internal standard.

If the system runs into an error state, the results for the
processed sample should not be trusted.
EMC caution statement
This device complies with the emission and immunity
requirements described in the IEC61326 standard.
Do not use this device in close proximity to sources of strong
electromagnetic radiation (e.g. unshielded intentional radiators),
as these can interfere with proper operation.

8
If this device is integrated into a system, the user is responsible
for electromagnetic compatibility of the overall system.

FCC emission notice

This device complies with Part 15 of the FCC Rules. Operation
is subject to the following two conditions: (1) this device may not
cause harmful interference, and (2) this device must accept any
interference received, including interference that may cause
undesired operation.
However, there is no guarantee that interference will not occur in
a particular installation.
Changes or modifications not expressly approved by the party
responsible for compliance, could void the user’s authority to
operate the equipment. Only use this device with the specified
cables.

Canada spectrum management emissions notice

This class A digital product apparatus complies with Canadian
ICES-001.
Cet appareil numérique de la classe A est conforme à la norme
NMB-001.

ISM Classification
This device has been classified as ISM Group 1 Class B in
accordance with IEC CISPR 11 Industrial Scientific and Medical
(ISM) instruments requirements.
Class B products are suitable for use in both commercial and
residential locations and can be directly connected to a low
voltage, power-supply network.

Korean EMC notice

This device complies with MSIP (Ministry Of Science, ICT, and
Future Planning) EMC registration requirements. This instrument
is registered as a Class B instrument for residential and/or
business use.

B급 기기 (가정용 방송통신기자재) 이 기기는 가정용(B급)

전자파적합기기로서 주 로 가정에서 사용하는 것을 목적으로
하며, 모든 지역에서 사용할 수 있습니다.

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2 Identification of parts

2.1 Instrument - front

Caution!
The inside the oven compartment may be hot!

Make sure the temperature in the oven compartment has

decreased to a safe value before opening the compartment.

Figure 1: Column oven - front

1. Status LED
2. Diverter valve (optional)
3. Display and keypad
4. Fans; do not obstruct!
5. Door handle
6. Leakage drain
7. Column bridge
8. Preheater
9. Column selection valve (optional)

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2.2 Instrument - rear

Figure 2: Column oven - rear

1. USB (optional)
2. Ethernet (optional)
3. OUT (serial)
4. IN (serial)
5. I/O connector
6. Ventilation holes; do not obstruct!
7. Fuse box, power connector, ON/OFF switch, voltage selector

2.3 Supply line and preheater

The oven compartment contains a preheater. The preheater (figure 3, number 1) is

mounted to the back plate of the oven compartment; the supply line can be led

11
through the door seals and along the chase at the back of the preheater to the
column, or to the valve (if present).

Figure 3: Preheater and supply line

Execute the following steps if you need to install a new supply line:

1. Make sure the temperature within the compartment is 25°C to prevent injuries.

2. Open the column oven door.

3. Remove the two screws that fasten the preheater (figure 3, number 2).

4. Carefully remove the preheater.

5. Unscrew the supply line from the valve (if valve option is installed) or column.

6. Mount a new supply line (figure 3, number 3) and connect it to the valve (if
valve option is installed) or column.

7. Lead the supply line along the chase in the preheater as shown in figure 3.

8. Fasten the preheater again with the two screws.

9. Lead the supply line over the door seals to the supplying instrument.

10. Flush the supply line and close the column oven door.

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2.4 Options

The following options are available for the column oven:

• Column selection valve
Valve for multiple LC column use This factory-installed option allows you to use
the column oven with a maximum of 6 different LC columns.

Figure 4: Valve for use of multiple columns

• no-cool version of the column oven. It is not possible to control a decrease in

temperature with the no-cool version. Refer to the specifications for more
information.
• Diverter valve
A factory-installed valve on the outside of the instrument.
This valve allows you to divert the LC flow to waste, after the analytes have
entered the mass spectrometer. This prevents contamination of the Mass
Spectrometer.

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3 Control software

The instrument is controlled by ServiceLink control software.

Refer to the User manual for information on software installation, instrument

configuration and general use.

3.1 Service Menu

Open ServiceLink. Select (TAB) Direct Control and select the arrow next to the
instrument to open the popup menu.

Figure 5: ServiceLink – popup menu

Select the Service menu option in Figure 5.

Figure 6: ServiceLink – Service code login

Enter service code: 101163 and select [OK].

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3.1.1 Service

Figure 7: ServiceLink – Service menu

Temperature Temperature setpoint

Control Enter the desired temperature.
Temperature range with cooling: 5 - 90˚C*
Temperature range with cooling: 25 - 90˚C*

* The maximum temperature is limited to 70˚C when the

Column selector (switch) option is available.

Temperature(˚C)
Current temperature in the oven compartment.
Fan Speed (rpm)
Current rotational speed of the oven fan.

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Column selector Valve position
Currently selected position.
(optional)
Log counter (wetted)
Valve log counter. Select [Reset] to zero the counter.
(the information will be lost!)
Log counter (mechanical)
Valve unit log counter. Select [Reset] to zero the
counter. (the information will be lost!)
Sensor home
Sensor reading: Interrupted: 1,2,3,5,6 Not interrupted: 4
Sensor legal
Sensor reading: Not interrupted:

Diverter valve Valve position

Currently selected position.
(Optional)
Log counter (wetted)
Valve log counter. Select [Reset] to zero the counter.
(the information will be lost!)
Log counter (mechanical)
Valve unit log counter. Select [Reset] to zero the
counter. (the information will be lost!)
Sensor 1-2
Sensor reading: Not interrupted (1-2), Interrupted (6-1)
Sensor 6-1
Sensor reading: Interrupted (1-2), Not interrupted (6-1)

Vapour alarm Set the Vapour alarm sensitivity.

sensitivity Low, Standard, High

Identification Blinking the instrument LED for identification purposes.

LED [Start identification] / [Stop identification]

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3.1.2 Production

Figure 8: ServiceLink – Production menu

Serial number Serial number

Enter the instrument serial number. Select [Save] to
store.
Temperature(˚C)
Current temperature in the oven compartment.
Fan Speed (rpm)
Current rotational speed of the oven fan.

Part number PCB part number / Revision

Enter the PCB part number and revision. Select [Save]
to store.

Calibration Offset at 10˚C*

values Calibration (offset value)
* Only available when cooling option is present
Offset at 30˚C
Calibration (offset value)
Offset at 90˚C
Calibration (offset value)
Select [Save] to store.

Maximum valve Set the maximum valve pressure.

pressure Select [Save] to store

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 Vapour alarm Set the vapor alarm value.
calibration Select [Save] to store

Installed options Select the installed instrument options.

Do not activate options that are not physically installed!
Select [Save] to store.

3.1.3 USB program

Figure 9: ServiceLink – USB program menu

USB program Set file

Browse to and select the *.Set file to be uploaded to the
instrument.
Serial number
Displays the instrument serial number.
After PCB replacement, update this information and
confirm with [Program].

3.1.4 Flash upload

Figure 10: ServiceLink – Flash upload menu

Upload firmware Set file

flash file Select [Select] and browse to the *. flash file to be
uploaded to the instrument.
Select [Upload] to start the upload sequence.
The upload status is displayed during upload.

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3.1.5 System information

Figure 11: ServiceLink – System information menu

The system information menu provides information about Firmware, FPGA

versions, Serial number and installed hard/software versions.

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4 Maintenance

Note that this section only describes basic maintenance procedures.

Temperatures inside the oven compartment of the column oven

may be high. Make sure the actual temperature inside the
compartment has decreased to a value of e.g. 25°C before you
open the oven compartment door to prevent injuries.

4.1 Column selection valve - rotor seal replacement

Rotor seal replacement only applies to the HPLC version of the selection valve.

Do not disassemble the UHPLC version of the valve!

This results in irreparable damage to the valve

As supplied, all surfaces are clean and free of contaminants and must be kept
clean to prevent valve damage. Prevent particulate matter from entering the valve
to prevent scratching of the sealing surfaces.

Execute the following steps to replace the Column selection valve - rotor seal:

1. Disconnect the tubing from the valve.

2. Remove the 3 socket head screws that fasten the stator.

3. Gently remove the stator. Place it on the outer face to prevent damage.

4. Take out the rotor seal.

5. Place the new rotor seal. The pattern is asymmetrical to prevent improper
placement. Make sure the engraved flow passages are visible!

6. Clean the stator with an appropriate solvent, examine the stator for
scratches.

7. Place the stator back, use the guide pin in the valve body for orientation.

8. Re-install the three socket head screws and fasten them alternately.

9. Reconnect the tubing to the valve.

10. Switch the valve.

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4.2 Diverter valve - rotor seal replacement

As supplied, all surfaces are clean and free of contaminants and must be kept
clean to prevent valve damage. Prevent particulate matter from entering the valve
to prevent scratching of the sealing surfaces.

Execute the following steps to replace the Diverter valve - rotor seal:

1. Disconnect the tubing from the valve.

2. Remove the three socket head screws that fasten the stator (Figure 12).

3. Gently remove the stator. Place it on the outer face to prevent damage.

4. Take out the rotor seal.

5. Place the new seal on the two guide pins. Make sure the engraved flow
passages are visible.

6. Clean the stator with an appropriate solvent, examine the stator for
scratches.

7. Place the stator back, use the guide pin in the valve body for orientation.

8. Re-install the three socket head screws and fasten them alternately.

9. Reconnect the tubing to the valve.

10. Switch the valve.

Figure 12: Diverter valve - exploded view

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4.3 Diverter valve - valve replacement

Execute the following steps to replace the Diverter valve:

1. Disconnect the tubing from the valve. Remember the valve shaft position!

2. Loosen the two M4 screws. Remove the valve assembly.

3. Remove the two 8-32 x 3/8 screws from the black plastic connector plate
(Figure 12).

4. Remove the black plastic connector plate including the metal plate holding
the M4 screws.

5. Make sure the position of the new valve shaft is identical to that of the old
valve. Correct the position by hand if necessary.

6. Mount the black plastic connector plate including the metal plate holding the
M4 screws to the new valve.

7. Place the valve assembly in the instrument. Refer to Figure 4 for orientation
of the valve.

8. Fasten the two M4 screws.

9. Re-connect all tubing to the valve.

10. Switch the valve.

4.4 Replacing fuses

Execute the following steps to replace fuses of the column oven:

1. Disconnect the column oven from the power source by removing the power
cord.

2. Use a flat head screw driver to slide out fuse box.

3. Replace fuses with fuse rating identical to the type you removed (refer to
section 11 Specifications).

22
5 Trouble shooting

Refer to the User manual for trouble shooting procedures.

6 Disassembly

Unplug the instrument before accessing the

inside!

6.1 Removing the left side panel

To get access to the assemblies inside the column oven, remove the left side
panel. Proceed as follows:

1. Disconnect the column oven from the mains.

2. Remove the 4 Phillips screws marked A from the front left-hand side.

23
3. Remove the 4 Phillips screws marked B from the rear right-hand side.
B

4. Slide the left side panel carefully off the mainframe, starting at the bottom as
shown below:

24
6.2 Inside the column oven

The following parts can be identified inside the column oven:

1. Power supply unit 24VDC

2. Net filter selector

3. Heater fan motor

4. Peltier cooling assembly

5. Main PCB

6. Column selection valve

7. Keypad/display flat cable

8. Wiring Temperature/Vapour sensor.

9. Cooling fans. 6 10

10. Diverter valve

6.3 Heater fan motor replacement

For replacing the Heater fan proceed as follows:

25
1. Remove the left side panel as described in the section 9.1.

2. Remove two Phillips screws marked C from the Pre-heater.

3. Remove the Pre-heater.

4. Remove the 8 Phillips screws marked D to remove the column plate.

D D

D D

D
D

D D

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 5. Disconnect connectors J16 and J17 from the main PCB and pull the wires back
through the insulation foam of the column compartment.

J17
J16

6. Remove the column plate including the disconnected wiring from the column
compartment. The assembly is displayed below.

27
7. Remove the heater fan using a 2mm hex screw driver.

8. Slide the heater fan from the axle.

9. Remove the net filter selector from the frame work.

28
 10. Remove the 4 Phillips screws from the heater fan motor.

11. Disconnect the connector from the motor.

12. Reverse the procedure to re-install the heater fan.

6.4 Peltier cooling assembly replacement

Proceed as follows to replacing the Peltier cooling assembly:

1. Follow the Heater fan replacement instruction until step 9.

2. Remove the Phillips screws marked E from the cooling plate.

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 E

E E

E E

E E

E E

3. Remove the bolts and nuts marked F that keep the tunnel plates in place on
the heat sinks.

F
F

J12

F
F

4. Remove connector J12 from the main PCB as marked in the picture above.

5. Slide the Peltier cooling assembly out of the front oven compartment.

30
 6. The new assembly has a yellow connector mounted on the wires and two
loose connectors are included in the packaging.

Install the new Peltier cooling assembly in the reversed order.

Check J12 on the main PCB. If this is a green connector, cut off the yellow
connector and strip the red and black wires for 10mm. Connect the wires to the
provided green connector, the colors are mentioned on the PCB.

6.5 Main PCB replacement

Different versions of main PCB’s were delivered in the Column oven. These PCB’s vary in
available Communication and Option connections.

A new replacement PCB will be equipped with all Communication and Option connections.
There will be no difference in functionality compared to the old PCB! As functionality of the
various connections is determined by the Firmware.

Note: e.g. if the old PCB was not equipped with an Ethernet connector, the
Ethernet connector will not function on the new PCB.

Contact your distributor for PCB part number information (including firmware).

See chapter 0: Main PCB’s for more information.

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For replacing the Main PCB, the instrument has an easy access on the rear side of
the instrument. For replacing the Main PCB proceed as follows:

1. Remove the 3 Phillips screws marked G on the rear side of the instrument.

2. Remove all the connectors connected to the Main PCB and slide it out of the
instrument.

3. Remove the screw locks and the 4 Phillips screws that keep the main PCB in
place.

4. Use the reversed order of steps to install the new Main PCB.

5. On the column oven keypad press both, the arrow key right and arrow key up
simultaneously and switch on the Column oven. Use the up- and down arrow
to navigate through the temperature calibration. Copy the 3 calibration points
for a column oven with cool option and 2 for a column oven without cool option
from the values located on the calibration label into the system settings.

32
 or

6. Select the installed options in the system settings.

33
6.6 Sensor PCB replacement

For replacing the Sensor PCB proceed as follows:

1. Follow the Heater fan replacement instruction until step 7.

2. Remove the Sensor PCB from the column plate and mount the new Sensor
PCB as displayed in the picture below.

3. Install the column plate back in reversed order.

4. On the column oven keypad press the arrow key right and the arrow key up
simultaneously and switch on the Column oven. Use the up and down arrow
keys to navigate through the temperature calibration. Set the 3 calibration
points for a column oven with cool option and 2 for a column oven without cool
option to the default values 10.0, 30.0 and 90.0.

5. Remove the column selection valve if a column selection valve is installed. The
temperature is limited in that case. See the Column selection valve
replacement instruction.

6. Before calibrating: Close the opening (left by the Column selection valve) with
a piece of foam.

34
7. Place the thermometer in the center of the oven compartment, 200 mm from
the bottom of a vertically placed column oven. Prevent contact of the
thermometer sensor with metal parts. Temperature measurement can be
performed with or without columns in place.

8. Set the temperature to the desired set point 10.0, 30.0 and 90.0 on the keypad
and switch on temperature control.

9. Leave the temperature to stabilize for at least 30 minutes at each set point
(10.0, 30.0 and 90.0)

10. Record the temperature by reading out the Thermometer.

11. After measuring the 3 or 2 calibration set points, enter these values in the
system settings. Refer to chapter 7.2 Temperature calibration check.

12. Install the Column switching valve.

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6.7 Column selection valve assembly replacement

For replacing the Column selection valve assembly proceeds as follows:

If the diverter valve option is installed:

First remove the diverter valve assembly as described in section 6.8 prior to
removing the Column selection valve.

If the diverter valve option is not installed:

1. Remove the 2 Phillips screws marked H for removing the top cover from the
column oven.

H H

K K

2. Remove any columns out of the column compartment and disconnect the
column selection connector from the main PCB (J18).

3. Remove the column selection valve by removing the two Phillips screws
marked K.

4. Replace the column selection valve assembly and install it in reversed order.

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6.8 Diverter valve assembly replacement

For replacing the diverter valve assembly proceeds as follows:

1. Remove all tubing from the diverter valve.

2. Switch the valve to position 1-2.

3. Loosen the two marked screws next to the valve. Remove the valve.

(NOTE: ports 1 & 2 are on top).

4. Instrument rear: Remove 5. Instrument front: Remove the 4 marked

the 4 marked Phillips Phillips screws.
screws.

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6. Slide the side panel 7. Slide the panel carefully 10 cm to the rear of
carefully off the the instrument.
mainframe, starting at
The panel stays attached by the fan cables.
the bottom as shown.

8. Remove the Diverter valve cable (J27) from the main PCB.

9. Remove the three indicated screws and remove the diverter valve
assembly.

10. Install the new diverter valve assembly in reversed order.

38
11. Before placing the valve, switch on the instrument. The instrument switches
the diverter valve assembly to position 1-2.

12. Check if the pin is in the indicated position (1-2)

13. Mount the valve, make sure ports 1-2 are on top.

14. Reconnect the tubing.

Port.
1. from column / manifold
2. to waste
3. blind plug
4. blind plug
5. blind plug
6. to mass spectrometer

39
6.9 Front panel display ASSY replacement

Proceed as follows to replace the Front panel display:

1. Switch off the instrument.

2. Remove the Diverter valve assembly (if installed) as described in section 6.8.

3. Remove the Column selection valve (if installed) as described in section 9.7.

4. Disconnect the flat cable connector (J9) from the main PCB.

5. Remove the 4 Phillips screw marked L.

L L

L L

6. Remove the Front panel display assembly from the column oven.

40
7 Performance inspection

This chapter describes how to perform a SOP check and a Temperature calibration
check.

7.1 SOP check

This procedure describes the temperature performance in the column oven

during warming up and cooling down.

To test the performance a build in temperature program will automatically be

executed.

1. On the column oven keypad, press the arrow key up and switch on the column
oven. SOP TEST will be displayed.

2. Press SET to start the temperature program. After finishing of the program, a
temperature rise and temperature decrease value will be displayed.

Successful SOP performance:

Heating up > 9 degrees C / min (with cool option)
Cooling down > 2 degrees C/ min (with cool option)

7.2 Temperature calibration check

Record the calibration point from the label at the rear of the instrument or the
bottom of the column oven keypad. There will be 3 calibration points for a column
oven with cool option and 2 for a column oven without cool option.

Switch off the instrument. Press and hold buttons and simultaneously on
the keypad when switching on the instrument. Use the up and down arrow keys to
navigate through the temperature calibration. Verify the settings with the recorded
setting.

This procedure describes measurement of the temperature in the column

oven.
Temperature range: +5°C up to 90°C, with 1°C increments, with cool option.
Ambient + 5°C up to 90°C, with 1°C increments, without cool option.

Thermometer

41
The thermometer should have an accuracy of +/- 0.1 °C and is preferably of glass.
A digital thermometer suitable for measurements in air can be used as an
alternative.

• It takes the average thermometer 2 minutes to display the

correct temperature.
• By using an electronic thermometer heat may be conducted
from the outside to the inside of the oven. This may also
result in a reading that is incorrect. Use sufficient wire in the
compartment to minimize this effect or place the complete
thermometer inside the compartment.

Procedure

1. Place the thermometer in the center of the oven compartment; 200 mm from
the bottom of a vertically placed column oven. Prevent contact of the
thermometer sensor with metal parts. Temperature measurement can be
performed with or without columns in place.

2. Set the temperature to the desired set point on the keypad and switch
temperature control on.

3. Wait for the temperature to stabilize.

4. Record the temperature.

Successful temperature performance:

Accuracy: set point ± 1°C

The acceptable accuracy of the experiment includes the accuracy of the

temperature set point plus the accuracy of the thermometer.

42
Appendix A. Spares and accessories

The following spare parts are available for the column oven.

Component Description
0043.160 Insulation ring
0044.168 Column spring
0044.181 Front panel top
0044.192 Flat panel
0044.198 Preheater bottom
0044.199 Preheater top
0050.145 Label Mistral
0060.068 Manual Mistral Symbiosis
0060.070 Column oven service manual
0700.004 Valco ferrule 1/16" pck/10
0700.005 Valco nut 1/16" pck/10
0750.807 Wiring LED
0880.752 Peltier ASSY (replacement)
0880.605 Sensor PCB ASSY
0880.790 Shipping box assembly Mistral
0886.061 Ship kit Mistral Symbiosis CS
0886.300 Door ASSY
0886.301 Selection valve 886
0886.304 Column compartment
0886.318 Valve unit diverter valve
0886.401 Front panel display ASSY
0886.602 Mistral 886 display PCB ASSY
0886.701 Heater fan motor ASSY Mistral
0886.702 6 port selection valve 0.4 SS with pin
0886.803 Flat cable display
0886.814 Wiring mains inlet
0886.815 Wiring ground cable mains inlet
2002.302 Valco manifold Z6M1
2002.500 Diverter valve
2002.501 Rotor seal for diverter valve
2032.052 Feet
2033.059 Toothed belt 600 MXL025
2034.008 Pulley 18DF MXL025 Alu bore 6.35
2034.009 Pulley PLB 60MXL025 / 6WF-6
2147.151 Hinge
2147.152 Door lock
2524.819 Mains inlet switch/fuse holder
0886.769 Power supply replacement kit
2562.364 Sensor opto logic KIT5031C
2570.041 Cable Multilink 9p. Male/Female
0700.419 Stepper motor kit KH56JM2U079J
0700.420 Stepper motor kit KH56JM2U080J
2635.195 I/O connector 10 pins
2655.501 Fuses 5,0TL (UL) 250V
2657.001 Limit switch Microtherm 125 C
43
 2682.005 Papst Fan 8414
2998.127 Black cap 44mm
3796.139 Stainless plug 1/16"
3796.152 Rotor seal for HPLC-version of selection valve
3796.153 Stator for HPLC-version of selection valve

Note that various types of maintenance contracts for preventive maintenance are
available. Contact your distributor for more information.

44
Appendix B. Error codes
Code Cause
05 Communication error or
general system error.
06 Valve position error.
Something has gone wrong
during valve switching.
010 Vapour alarm
021 Actual Temperature <2˚C
022 Actual Temperature >95˚C
023 Incorrect temperature change
during warming up
030 Column selector error caused
by switching error in the
column selector valve.
050 Heater fan speed too slow due
to a mechanical defect.
Action
Switch the column oven off, and on
again. Call service if the problem
persists.
Switch the column oven off, and on
again. Initialize.
Check wiring from column switch to
Main PCB.
Check optical sensors using
ServiceLink.
Check movement without stator
installed. If the error disappears the
Torque on the valve is too high.
Replace the rotor seal.
Check for column leaks and vapor
sensor sensitivity.
Check temperature sensor
Check temperature sensor
Check heater elements
If the actual temperature is below 80
˚C. The temperature control is on full
power and no temperature change of
more than 2 ˚C occurs within 1 minute
(e.g. when the oven door is not closed
(air leaks)).
If the actual temperature is above 80
˚C. The temperature control is on full
power and no temperature change of
more than 0.3˚C occurs within 1 minute
(e.g. when the oven door is not closed
(Air leaks)).
Check the door for air leaks.
Switch the column oven off, and on
again. Initialize.
Check for mechanical obstructions.
Check wiring from column switch to
Main PCB.
Check optical sensors using
ServiceLink.
Check movement without stator
installed. If the error disappears the
Torque on the valve is too high.
Replace the rotor seal.
Check heater fan for blockage. Check
wiring from heater fan to Main PCB.
Check Main PCB.
45
060 Diverter Valve Error. Switching Switch the column oven off, and on
error in the Diverter valve. again. Call service if the problem
persists.
100 Check set points Programmed temperature exceeds
maximum system setting temperature.

46
Appendix C. Storage, packaging, recycling and disposal

If the column oven needs to be stored, or if it must be shipped to a different

location, proceed as follows:

1. Thoroughly flush the system.

2. Switch off the column oven.

3. Disconnect and remove all tubing. In case biohazard solvents were used:
throw away all tubing in accordance with an approved waste disposal program.

4. If the column oven needs to be dispatched: use the original packaging

materials to package the column oven and dispatch the package; make sure
any tax/import/export requirements are met.

Decontamination
The instrument shall be decontaminated before decommissioning and all local
regulations shall be followed with regard to scrapping of the equipment.

General instructions for disposal

When taking the instrument out of service, the different materials must be
separated and recycled according to national and local environmental regulations.

Hazardous substances
The instrument contains hazardous substances. Contact the manufacturer for more
detailed information.

Disposal of electrical components

Waste of electrical and electronic equipment must not be disposed as unsorted
municipal waste and must be collected separately. Please contact an authorized
representative of the manufacturer for information concerning the decommissioning
of equipment.

47
 Appendix D. PCB Layouts

This section offers exploded views and layouts of previous delivered PCB’s.

Main PCB’s
The column oven is controlled by one Main PCB. Three version PCB’s were delivered with
the instruments:
1. COM (serial) only.
2. COM (serial) & Ethernet.
3. COM (serial) & Ethernet + Diverter valve.
A new replacement PCB will be equipped with all connections see #3.
Contact your distributor for PCB part number information (including firmware).

COM (serial) only

PCB layout with descriptions of the available connectors.
J24 J25 J9
J23

J22
J26

J21

J20

J12 J19 J13 J14 J15 J16 J17 J1 J18

Connector # Description Connector # Description
J1 Power LED J19 Power connector 24 VDC
J9 Display connector J20 Heater element
J12 Peltier cooling ASSY J21 Heater element
J13 Cooling fan 2 J22 Mains power connection-1 (High voltage)
J14 Cooling fan 1 J23 I/O connector
J15 Heater fan J24 Serial IN
J16 Vapour sensor J25 Serial OUT
J17 Temperature sensor J26 Mains power connection-2 (High voltage)
J18 Column selection valve

48
 COM (serial) & Ethernet
PCB layout with the additional Ethernet connector.
M2

Connector # Description
M2 Ethernet connection

COM (serial) & Ethernet + Diverter valve

PCB layout with additional Ethernet, USB and Diverter valve connectors.

M2 J7 J27

Connector # Description
M2 Ethernet connection
J7 USB connector
J27 Diverter valve connector

49
 Appendix E. Exploded views

Door Assembly
0886.300 Door Assembly

Component Description Quantity

0044.179 Inner panel door 1
7150.303 Sealing strip 1
0043.116 Door insulation 1
2291.234 Distance bush 20x8 5
2147.151 Hinge 2
0044.178 Door cover painted 1
2147.152 Door lock 1
0044.180 Locating plate inner panel 1

50
Exploded view of Door Assy

51
Column oven-1
SP886.001 Column oven-1

Component Description Quantity

0044.175 Side panel bottom 1
0886.769 Power supply replacement kit 1
2524.819 Mains inlet switch/fuse holder 1
Mistral 886 PCB 1
0044.183 PCB bracket 1
0044.185 Fan holder 1
2682.005 Papst Fan 8414 2
0044.176 Side panel right* 1
6416.130 Screw SS 8
2147.153 Ventilation Grill 2
0886.300 Door ASSY 1
0044.110 Spacer hinge 2

* In case of diverter valve option:

0046.177 Side panel right DV 1

52
Exploded view of Column oven-1

53
Column oven-2
SP886.001 Column oven-2

Component Description Quantity

2998.127 Black cap 44mm 3
0044.177 Side panel left 1
0044.169 Drain back panel 1
0044.174 Side panel top 1
0886.301 Selection valve 886 1
0044.181 Front panel top 1
0050.145 Label Mistral 1
0044.195 Valve cover (with column selector is installed) 1
0044.200 Cover (without column selector installed) 1
0044.199 Preheater top 1
0044.198 Preheater bottom 1
0043.172 Insulation block 3
0043.159 Side valve insulation 3
7150.305 Sealing strip 1.1m

54
Exploded view of Column oven-2

55
Selection valve 886
0886.301 Selection Valve 886

Component Description Quantity

0886.702 6 port selection valve 0.4 SS with pin 1
0044.184 Plate 6-way Mistral 1
6410.044 Screw UNCX 1/2"x4.8 2
0045.301 Sensor plate 1
0045.302 Pulley shaft 1
2562.364 Sensor opto logic KIT5031C 2
0045.303 Sensor code disc 1
2033.059 Toothed belt 600 MXL025 1
2291.228 Bush 7.5x R12 8ID 1
2034.009 Pulley PLB 60MXL025 / 6WF-6 1
2291.254 Distance bush M4x 7x 50 3
2099.041 Nylon bearing 5mm 1
6416.014 Screw SS M3x6 1
0700.420 Stepper motor kit KH56JM2U080J 1
2034.008 Pulley 18DF MXL025 Alu bore 6.35 1
0045.304 Plate motor 1
6416.015 Screw SS M3x8 1
6096.383 Pin 1.5x10 1
6050.066 Set screw M6x6 DIN 913 1

56
Exploded view Selection valve 886

57
Valve unit Diverter valve

0886.318 Valve unit diverter valve

Component Description Quantity

0045.575 Base frame 1
0045.576 Valve lever 1
0045.582 Rocker guide 840 1
0840.312 Rocker arm ASSY 1
0886.816 Wiring Diverter Valve 1
2099.041 Nylon bearing 5mm 1
2100.031 Ball bearing 61803-2Z 1
2562.364 Sensor opto logic KIT5031C 2
0700.419 Stepper motor kit KH56JM2U079J 1
6062.203 Self-locking nut M3 1
6405.051 Bolt hexagon SS M5x10 1
6416.024 Screw SS M4x8 2

58
Exploded view Valve unit diverter valve

59
Column compartment
0886.304 Column Compartment

Component Description Quantity

0886.701 Heater fan motor ASSY Mistral 1
7200.405 Wire strip 2
0044.187 Fame extended Mistral 1
2657.001 Limit switch Microtherm 125 C 2
0880.752 Peltier ASSY (replacement) 1
0880.605 Sensor PCB ASSY 1
2291.234 Distance bush 2
0043.120 Column plate 1
0043.139 Column bridge 2
2682.004 Fan blade 1
6050.046 Set screw 1

60
Exploded view Column compartment

61
Front panel display ASSY
0886.401 Front panel display ASSY

Component Description Quantity

0044.192 Flat panel 1
0044.189 Front panel display 1
0886.602 Mistral 886 display PCB ASSY 1
0886.803 Flat cable display 1

62
Exploded view Front panel display ASSY

63
Appendix D. Programming overview

64
Index

A F
About this manual • 5 Front panel display assy • 62
Accessories • 43 Front panel display assy replacement •
Alarms • 23 40
Appendix C. Storage, packaging, Fuse replacement • 22
recycling and disposal • 47 H
Appendix D. PCB Layouts • 48 Heater fan motor replacement • 25
Appendix E. Exploded views • 50 I
C Identification of parts • 10
Column compartment • 60 Inside the column oven • 25
Column oven-1 • 52 Instrument - front • 10
Column oven-2 • 54 Instrument - rear • 11
Column selection valve M
Rotor seal replacement • 20 Main PCB replacement • 31
Column selection valve assembly Main PCB’s • 48
replacement • 36 Maintenance • 20
Control software • 14 N
D No-cool version • 13
Disassembly • 23 O
Diverter valve Option
replacement • 22 Diverter valve • 13
Rotor seal replacement • 21 Options • 13
Diverter valve assembly replacement • P
37 Peltier cooling assembly replacement •
Diverter valve option • 13 29
Door Assy • 50 Performance inspection • 41
E Pictorials • 5
Error codes • 45 Preheater • 11
Exploded view Column compartment Programming overview • 64
• 61 R
Exploded view Diverter valve • 59 Removing the left side panel • 23
Exploded view Front panel display Replacing fuses • 22
assy • 63 Rotor seal replacement
Exploded view of Column oven-1 • 53 Column selection valve • 20
Exploded view of Column oven-2 • 55 Diverter valve • 21
Exploded view of Door Assy • 51 S
Exploded view Selection valve 886 • Safety instructions • 6
57 Selection valve 886 • 56

65
Sensor PCB replacement • 34 Trouble shooting • 23
SOP check • 41 V
Spare parts • 43 Valve multiple columns • 13
Supply line and preheater • 11 Valve unit Diverter valve • 58
T Vapour alarm • 23
Temp alarm • 23 W
Temperature calibration check • 41 Warnings • 23

66