Figure 1(a)

15 H. fossilis C. batrachus
d c c

WBC (X 104 mm3)

c b
10 b a
a

10 gL -1
20 gL -1

10 gL -1
20 gL -1

1
gL -

gL -
1 ro l

1 ro l
t

t
on

on
m

m
m
m

m
m
C

C
Different concentrations of EBT

Figure 1(b)

Experimental Alterations in RBC Count in Experimental Alterations in WBC Count in

Fish comparison to control group Fish comparison to control group
1 mg/l 10 mg/l 20 mg/l 1 mg/l 10 mg/l 20 mg/l
H. fossilis ↓23.37% ↓30.31% ↓43.02% H. fossilis ↓20.26% ↓30.51% ↓35.35%

C. batrachus ↓16.39% ↓41.77% ↓49.42% C. batrachus ↑22.57% ↑35.05% ↑36.71%

Figure 2(a)

Figure 2(b)

Experimental Alterations in Hb Content in Experimental Alterations in Ht Content in

Fish comparison to control group Fish comparison to control group

1 mg/l 10 mg/l 20 mg/l 1 mg/l 10 mg/l 20 mg/l

H. fossilis ↓21.89% ↓22.46% ↓40.27% H. fossilis ↓19.54% ↓18.49% ↓37.01%

C. batrachus ↑12.06% ↑10.67% ↑19.59% C. batrachus ↑8.25% ↑7.5% ↑22.92%

Figure 3
Figure 4
Figure 5
H. fossilis
Control 1 mgL-1 10 mgL-1 20 mgL-1

MD
DN
NC
ExN
DN MD
DC ExN ExN
MN
EcN DC
MD
MD

C. batrachus
Control 1 mgL-1 10 mgL-1 20 mgL-1

DN DC
DC
ND
EcN
DC
NC EN
MN ExN
DN
VC

Abbreviations: NC: Normal cells, DC: Deformed cells, DN: Dividing nucleus, EN: Enucleation, EcN: Eccentric nucleus,
ExN: Extruding nucleus, MD: Membrane disintegration, MN: Micronucleus, ND: Nuclear disintegartion, VC: Vacuolated
cells
Figure 6
Figure 7
Figure captions
Fig. 1(a) Alterations in RBC and WBC counts in H. fossilis and C. batrachus after 96 hrs of exposure to different
concentrations of EBT. The data is shown as mean ± SD, and different superscripts indicate statistically significant
differences at p < 0.05

Fig. 1(b) The percentage change in RBC and WBC counts in H. fossilis and C. batrachus after 96 hours of exposure to
different concentrations of EBT. The downward arrow (↓) indicates a decrease in percent change from the control, and an
upward arrow (↑) indicates an increase in percent change from control

Fig. 2(a) Alterations in Hemoglobin (Hb) and Hematocrit (Ht) contents in H. fossilis and C. batrachus after 96 hrs of
exposure to different concentrations of EBT. The data is shown as mean ± SD, and different superscripts indicate
statistically significant differences at p < 0.05

Fig. 2(b) The percent change in Hb and Ht contents in H. fossilis and C. batrachus after 96 hrs of exposure to different
concentrations of EBT. The downward arrow (↓) indicates a decrease in percent change from the control, and an upward
arrow (↑) indicates an increase in percent change from control

Fig. 3 Effect of different concentrations of EBT on the Differential Leukocyte count of H. fossilis after 96 hrs of EBT
exposure

Fig. 4 Effect of different concentrations of EBT on the Differential Leukocyte count of C. batrachus after 96 hrs of EBT
exposure

Fig. 5 Effect of different concentrations of EBT on the structure of erythrocytes after 96 hrs of exposure in H. fossilis and C.
batrachus

Fig. 6 Principal component analysis biplot of haemato-biochemical parameters of H. fossilis treated with different
concentrations of EBT for 96 hrs. Figure comprises both location of EBT concentrations and distribution of parameters
according to their response

Fig. 7 Principal component analysis biplot of haemato-biochemical parameters of C. batrachus treated with different
concentrations of EBT for 96 hrs. Figure comprises both location of EBT concentrations and distribution of parameters
according to their response