Solvent Water Acetonitrile Methanol

Volume (µL) 1000 200 20 1000 200 20 1000 200 20

#1 1001.6 200.8 20 778.8 0 0 782.1 161.6 3.5

#2 1000.6 200.3 20 778.7 0 0 791.5 160.6 10.4

#3 1001.3 200.1 19.8 776 0 0 789.1 160 12.7

#4 1000.9 200.1 19.8 783.5 0 0 780.3 161 12.5

#5 1000 200.1 20.1 784.4 0 0 784.7 159.5 13.7

#6 1000.2 200.1 20.2 784 0 0 783 158.8 12.9

#7 1000.6 200.1 20 783.8 0 0 781.7 159.3 14

#8 1000.7 200.2 20.1 781 0 14.5 779.1 158.4 12.8

#9 1000.7 200.2 20.2 781.5 155.3 13.6 770.4 158 13.6

#10 1000.8 200.3 20.1 781.5 155 15 780.3 159.5 14.6

Mean 1000.74 200.23 20.03 781.32 31.03 4.31 782.22 159.67 12.07

SD 0.46714261 0.21628171 0.14181365 2.77400633 65.4170221 6.94781341 5.74761786 1.14605604 3.21733154

CV% 0.04667972 0.10801664 0.70800624 0.355041 210.818634 161.202167 0.73478278 0.71776542 26.6556051