Osmosis required practical

Equipment:
 Balance
 Scalpel
 Beaker
 6 test tubes
 Water bath (30 degrees)
 Potato
 0.8 mol dm -3 salt solution
 Syringe
 Marker pen
 Denoised water
 Timer
 Test tube rack
 Potato corker
 Tile

Method:
Begin by adding 40cm3 of the 0.8 mol solution to a beaker. Extract 20cm3 of
this and add it to a test tube labelled ‘0.8’. With the remaining 20cm3 we will
put an additional 20cm3 of deionised water to create a 0.4 mol dm-3 salt
solution, add 20cm3 of this to a test tube labelled 0.4 mol dm-3. Repeat the
method until you have solutions that are both 0.2 and 0.1 in labelled test
tubes. We can create a 0.6 mol dm-3 solution by adding 30cm3 of the 0.8
moldm-3 solution to a beaker and then adding 10cm3 of deionised water.
Once we have completed our prep, we need to add our test tubes to a water
bath set at 30 degrees Celsius for 10 minutes (Use stopwatch). In the
meantime, we can prepare our substrate samples. Collect your potato, tile and
corker – we are looking for 6 samples of potato each similar in size and mass.
Weigh each potato and record the initial weight on scrap paper. Then add each
sample to a test tube recalling which one went in which solution, for example
the sample that weighed 2.27g in the 0.4 moldm-3 solution, before placing
them all into the water bath for 20 minutes. Rerecord their masses after the 20
minutes then calculate percentage change in mass by dividing the difference
by the start mass. Using this dat we can plot a graph that includes both positive
and negative values – our x axis would be labelled ‘concentrate of solution mol
dm-3’ and our y axis ‘percentage change in mass %’.