MANISH BAIS, DVM, PhD

manish.bais@gmail.com W-216, 700 Albany Street, Boston MA 02118 PROFESSIONAL SKILLS: Oncology- Cancer xenografts, in-vitro co-culture cancer metastasis model, in vivo cancer metastasis mouse models for breast and prostate cancer (intra-tibial and intra-cardiac). Gene therapy, bone marrow stem cells manipulation and pre-clinical animal models. Cell/ Molecular techniques: Generation of recombinant DNA constructs and chimeric lentivirus particles, shRNA, sample preparation for microarray, PCR, Real Time PCR, Immunoprecipitaiton, pull down assay, Western Blot, Reporter assays, FACS, Packaging recombinant lentivirus particles, Propagation of recombinant adenovirus, cell cytotoxicity assays and anti-cancer efficacy assays. Pathology: Histology, Histomorphometry, quantitative Immunohistochemistry. Cell culture: Cancer cells, chondrocyte, osteoblast, stem cells, their differentiation and staining. Surgical skills- bone marrow ablation model, fracture healing model, injection of cells or lentivirus particles into tibia by intra-tibial injections, surgical implantation of cell graft, alginate beads or osmotic pump subcutaneously. Imaging- Advanced computer aided imaging system as IVIS 200 imaging system, fluoresce and confocal microscopy. Animal work- mice handling with breeding colony of transgenic, wild type and immune-deficient mice Local delivery of recombinant proteins and its stabilized formulations in animals. Experience with various routes of drug administration iv, ip, and sc. Designing in vivo and in vitro experiments Accepts challenges, self motivated, flexible and open to change, innovative and results oriented. Team work- Collaborate with other research group, Supervise fellow students and scientist, Good written and oral communication. Prepared and contributed to grant submission for NIH and DOD. Extensive experience with manuscript writing. RESEARCH EXPERIENCE Dec 2010- Present: Research Assistant Professor, Boston University. Tumor suppressor LOX-PP protein inhibits breast cancer xenografts project finished within only 2 years with publication. Identified LOX-PP interacting proteins for combinatorial radiation anti-cancer chemotherapy. Purification proteins in mammalian cells to study effect on cancer and cancer metastasis. identify candidate to .Inhibit cancer metastais. Teaching laboratory methods and courses to master and PhD students May 2009- Nov 2010 : Postdoctoral Research Associate in Goldman Dental School, Boston, MA. Project completed; LOXL2 in chondrogenesis as a co-author in paper (JBC 2010) in 1.5 yrs. Studied effect of anticancer protein LOX-PP on breast cancer xenograft Established models for prostate cancer metastsis to bone in vitro and in vivo and determine the effect of tumor suppressor protein LOX-PP. Sept 2004- Nov 2009 Postdoctoral fellow at Boston Medical Center. identified difference in mechanism of bone healing by BMP2 and BMP7 proteins and its clinical relevance. Identified Nanog, embryonic stem cell related gene is expressed in fracture healing and adult stem cells. Evaluated expression of genes related to osteogenesis, vasculogenesis, stem cells and cancer metastasis. AWARDS/HONORS Recipient of University Merit-cum-means Scholarship (1994-1998). Prestigious IVRI Junior Research Fellowship (JRF) for MVSc (1999-2001). Prestigious IVRI Senior Research Fellowship (SRF) for Ph.D (2001-2004).

EDUCATION Post-doctoral Fellow ( 2004-2010): Boston University with research in area of Musculoskeletal, cartilage, and cancer bone metastasis. Course on Drug Discovery, credit 4.0, GPA 4.0. PhD 2004: Animal Biotechnology, National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar, India. Thesis: Identification of Novel Nuclear Localization Signal from IBD Virus for Development of Peptide Based Delivery System. M.V.Sc.( 1999-2001): Animal Biotechnology, National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar, India. Thesis: Sequence Analysis of Very Virulent Infectious Bursal Disease Virus of Poultry. D.V.M. (1994-1998): Nagpur Veterinary College, Nagpur, MS, India US Grades Credential Evaluation Report by World Education Services, NY (www.wes.org) Total Graduate Semester credits (Master and PhD) = 87, GPA: 4.0 Total Professional Semester Credits = 150, GPA: 3.69. PROFESSIONAL MEMBERSHIPS American Society for Matrix Biology. American Association of Pharmaceutical Scientists. The American Society for Bone and Mineral Research. SCIENTIFIC PRESENTATIONS 1. Bais, et al, American Society for Matrix Biology Biennial Meeting Charleston, SC Oct 24-27, 2010 (# B130) 2. Bais et al, 31st American Society of Bone and Mineral Research, Colorado. September 14, 2009 (# 1168) 3. Bais et al, 55th Annual meeting of Orthopedic Research Society, Las Vegas, NV Feb 22-25, 2009 (# 129) 4. Marsell R., Bais M. et al., 31st Annual meeting Colorado Convention Center September 14, 2009 (# MO0219) 5. Bais et al, 55th Annual meeting of Orthopedic Research Society, Las Vegas, NV Feb 22-25, 2009 (#600) 6. Bais et al., 54th Annual Meeting of the ORS San Francisco, CA March 2-5, 2008 7. Bais et al., 30th ASBMR annual meeting Montreal, Canada Sept 12-16, 2008 8. Bais et al., 30th ASBMR annual meeting Sept 12-16, 2008 Montreal, Canada 9. Bais et al., Molecules, Machines and Network Banglore, India Jan 6-9, 2004. PATENT : An Artificially Synthesized Peptide. Dr. Satish Kumar, Dr. Manish Virendrasingh Bais, Dr. Ranjit Singh Kataria Dr. Mahendra Pal Yadav. Pub. No.: WO/2008/041047, International Application No: PCT/IB2006/002786, Publication Date: 10.04.2008. PUBLICATIONS 1. Bais, M.V., Nugent, M.A., Stephens, D.N. Sume, S.S. Kirsch, K.H. Sonenshein, G.E., Trackman, P. C. Intratumoral or Slow Release of rLOX-PP Inhibits Growth of Pre-existing Murine Breast Cancer Xenografts by Inhibiting Proliferation and Inducing Apoptosis ( PLoS ONE, In press). 2. Bais, M.V., Shabin Z.M., Young, M., Kotton, D.N., Einhorn, T.A., Gerstenfeld, L.C.(2011) Role of Nanog in Marrow Stromal Stem Cell Differentiation During Bone Repair (Biochem Biophy Res Comm, In press). 3. Iftikhar, M., Hurtado, P., Bais, M. V., Wigner, N., Stephens, D. N., Gerstenfeld, L. C., and Trackman, P. C. (2010) Lysyl oxidase-like-2 (LOXL2) is a major isoform in chondrocytes and is critically required for differentiation, J Biol Chem 286, 909-918 PMID: 21071451. 4. Bais, M. V., Wigner, N., Young, M., Toholka, R., Graves, D. T., Morgan, E. F., Gerstenfeld, L. C., and Einhorn, T. A. (2009) BMP2 is essential for post natal osteogenesis but not for recruitment of osteogenic stem cells, Bone 45, 254266 PMID: 19398045. 5. Bais, M., McLean, J., Sebastiani, P., Young, M., Wigner, N., Smith, T., Kotton, D. N., Einhorn, T. A., and Gerstenfeld, L. C. (2009) Transcriptional analysis of fracture healing and the induction of embryonic stem cell-related genes, PLoS One 4, e5393 PMID: 19415118. 6. Ravindra, P. V., Tiwari, A. K., Ratta, B., Bais, M. V., Chaturvedi, U., Palia, S. K., Sharma, B., and Chauhan, R. S. (2009) Time course of Newcastle disease virus-induced apoptotic pathways, Virus Res 144, 350-354 PMID: 19501124.

7. Bais, M. V., Kumar, S., Tiwari, A. K., Kataria, R. S., Nagaleekar, V. K., Shrivastava, S., and Chindera, K. (2008)
Novel Rath peptide for intracellular delivery of protein and nucleic acids, Biochem Biophys Res Commun 370, 27-32 PMID: 18346454. 8. Nagaleekar, V. K., Tiwari, A. K., Kataria, R. S., Bais, M. V., Ravindra, P. V., and Kumar, S. (2007) Bluetongue virus induces apoptosis in cultured mammalian cells by both caspase-dependent extrinsic and intrinsic apoptotic pathways, Arch Virol 152, 1751-1756 PMID: 17530353. 9. Bais, M. V., Kataria, R. S., Tiwari, A. K., Viswas, K. N., Reddy, A. V., and Prasad, N. (2004) Sequence analysis of an Indian field isolate of infectious bursal disease virus shows six unique amino acid changes in the VP1 gene, Vet Res Commun 28, 641-646 PMID: 15563111. 10. Indervesh, Tiwari, A. K., Kataria, R. S., Viswas, K. N., Bais, M. V., and Suryanarayana, V. V. (2003) Isolates of infectious bursal disease virus from India are of very virulent phenotype, Acta Virol 47, 173-177 PMID: 14658846. 11. Bais, M. V., Kataria, R. S., Tiwari, A. K., Indervesh, Suryanarayana, V. V., and Das, S. K. (2003) Sequence analysis of segment a of a field virus isolate from an outbreak of Infectious bursal disease in India, Acta Virol 47, 73-77 PMID: 14524472.