T F WO - ACTOR

By Hamza Bin Sajid

E
Department of Plant Breeding & Genetics
University of Agriculture Faisalabad
XPERIMENTS
hamzabinsajid786@gmail.com
Link of this complete Lecture
Description on YouTube:

https://youtu.be/DR4rIcEqs1E
Reference Book:

Chapter No. 3
Page # 84 - 129
INTERACTION BETWEEN TWO FACTORS –
Two factors are said to interact if the effect of one
factor changes as the level of the other factor
changes.
Suppose we are going to start an experiment with two
factors A and B. Both the factors have 2 levels. For A
(𝑎1 , 𝑎2 ) And for B (𝑏1 , 𝑏2 )

Reference Book
Chapter No. 3
Page # 84 - 89
Interaction is Present.
That’s an example of
Significant interaction
Interaction is absent.
That’s an example of non-
significant interaction
 POINTS TO BE NOTED –
1. An interaction effect between two factors can be measured
only if the two factors are tested together in the same
experiment (i.e., in a factorial experiment).
2. When interaction is absent the simple effect of a factor is
the same for all levels of the other factors and equals the
main effect.
3. When interaction is present the simple effect of a factor
changes as the level of the other factor changes.
Reference Book
Chapter No. 3
Page # 87 - 89
F
ACTORIAL E
By Hamza Bin Sajid
XPERIMENT
Department of Plant Breeding & Genetics
University of Agriculture Faisalabad
hamzabinsajid786@gmail.com
Definition:
An experiment in which the treatments consist of all
possible combinations of the selected levels in two or
more factors is referred to as a factorial experiment.
Drawback Example: Same used in previous
❑Avoid indiscriminate use of factorial experiments because
of their large size, complexity, and cost.
❑large experiment would mean difficulties in financing, in
obtaining an adequate experimental area, in controlling
Reference Book
soil heterogeneity, and so on. Chapter No. 3
Page # 89-91
Through the factorial experiments, we can study
- the individual effect of each factor and
- interaction effect.
T F
WO - ACTOR
EXPERIMENTS
BLOCK DESIGN
Reference Book
Chapter No. 3
Page # 91
Complete Block Design:
❑CRD
Completely Randomized Design
❑RCBD
Randomized Complete Block design
❑LSD
Latin Square Design
 T F WO - ACTOR
EXPERIMENTS
By Hamza Bin Sajid
BLOCK DESIGN
Reference Book
Department of Plant Breeding & Genetics
University of Agriculture Faisalabad Chapter No. 3
hamzabinsajid786@gmail.com Page # 91-97
Link of this complete Lecture on
YouTube:

https://youtu.be/bQAF5zT45Ds
Sample Question
 Sample Question
Factor A Factor B Replications

𝑏1
𝑏2
𝑎1
𝑏3
𝑏4

𝑎2
 Layout of 2 X 4 Factorial Design with Randomized
Complete Block Design with 4 Replications

𝑎1 𝑏1 𝑎1 𝑏2 𝑎1 𝑏3 𝑎1 𝑏4
Rep.1
𝑎2 𝑏1 𝑎2 𝑏2 𝑎2 𝑏3 𝑎2 𝑏4

𝑎1 𝑏4 𝑎1 𝑏3 𝑎1 𝑏2 𝑎2 𝑏4
Rep.2
𝑎2 𝑏2 𝑎1 𝑏1 𝑎2 𝑏1 𝑎2 𝑏3

𝑎1 𝑏3 𝑎1 𝑏4 𝑎2 𝑏2 𝑎2 𝑏4
Rep.3
𝑎2 𝑏1 𝑎2 𝑏3 𝑎1 𝑏2 𝑎1 𝑏1

𝑎2 𝑏4 𝑎2 𝑏2 𝑎1 𝑏1 𝑎1 𝑏2
Rep.4
𝑎1 𝑏4 𝑎1 𝑏3 𝑎2 𝑏1 𝑎2 𝑏3
 Reference
One Factor Experiment RCBD Page # 25

Analysis of Variance (ANOVA)

Source of Degree of Sum of Means F Tabular
Variation Freedom Square Square Calculated F
Replication/
Block
Treatment
Experimental
Error

Total
 Two Factor Experiment RCBD (ANOVA) Reference
Page # 93

Source of Degree of Sum of Means F Tabular

Variation Freedom Square Square Calculated F
Replication/
Block
Treatment
A
B
AXB
Experimental
Error
Total
 Two Factor Experiment RCBD (ANOVA) Reference
Page # 93-97

Source of Degree of Sum of Means F Tabular

Variation Freedom Square Square Calculated F
Replication/
(r-1)
Block
Treatment (t -1)
A (a -1)
B (b -1)
AXB (a -1)(b -1)
Experimental
(r-1)(ab-1)
Error
Total (rab-1)
 𝐺𝑇 2
𝐶𝑜𝑟𝑟𝑒𝑐𝑡𝑖𝑜𝑛 𝐹𝑎𝑐𝑡𝑜𝑟 (𝐶𝐹) =
𝑟𝑎𝑏
 Source of Degree of Sum of Means F Tabular
Variation Freedom Square Square Calculated F
Replication/ σ 𝑅2
(r-1) - CF
Block 𝑎𝑏
σ 𝑇2
Treatment (t -1) - CF
𝑟
σ 𝐴2
A (a -1) - CF
𝑟𝑏
σ 𝐵2
B (b -1) - CF
𝑟𝑎
AXB (a -1)(b -1) Treatment SS-ASS-BSS

Experimental Total SS - Replication

(r-1)(ab-1)
Error SS -Treatment SS

Total (rab-1) σ 𝑋 2 - CF
 Fertilizer (Factor B)
Check Aero Na K
Date of Early 130.7 118 111.1 117.1
Planting
Late 125.1 128.3 129.9 125.6
 F
Source of Degree of Sum of Means Tabular
Calcul
Variation Freedom Square Square F
ated
Replication/ σ 𝑅2 𝑅𝑒𝑝𝑙𝑖𝑐𝑎𝑡𝑖𝑜𝑛 𝑆𝑆
(r-1) - CF
Block 𝑎𝑏 𝑅. 𝐷. 𝑂. 𝐹
σ 𝑇2 𝑇𝑟𝑒𝑎𝑡𝑚𝑒𝑛𝑡 𝑆𝑆
Treatment (t -1) - CF 𝑇𝑟𝑒𝑎𝑡. 𝐷. 𝑂. 𝐹
𝑟
σ 𝐴2 𝐴 𝑆𝑆
A (a -1) - CF 𝐴. 𝐷. 𝑂. 𝐹
𝑟𝑏
σ 𝐵2 𝐵 𝑆𝑆
B (b -1) - CF 𝐵. 𝐷. 𝑂. 𝐹
𝑟𝑎
AXB (a -1)(b -1) Treatment SS-ASS-BSS 𝐴 𝑋 𝐵 𝑆𝑆
Experimental Total SS- Replication 𝐴 𝑋 𝐵.𝐷.𝑂.𝐹
(r-1)(ab-1)
Error SS -Treatment SS
𝐸𝑟𝑟𝑜𝑟 𝑆𝑆
𝐸𝑟𝑟𝑜𝑟. 𝐷. 𝑂. 𝐹
Total (rab-1) σ 𝑋 2 - CF
 Source of Degree of Sum of Means F Tabul
Variation Freedom Square Square Calculated ar F
Replication/ σ 𝑅2 𝑅𝑒𝑝𝑙𝑖𝑐𝑎𝑡𝑖𝑜𝑛 𝑆𝑆 𝑅𝑒𝑝𝑙𝑖𝑐𝑎𝑡𝑖𝑜𝑛 𝑀𝑆
(r-1) - CF
Block 𝑎𝑏 𝑅. 𝐷. 𝑂. 𝐹 𝐸𝑟𝑟𝑜𝑟 𝑀𝑆
Check F
σ 𝑇2 𝑇𝑟𝑒𝑎𝑡𝑚𝑒𝑛𝑡 𝑆𝑆 𝑇𝑟𝑒𝑎𝑡𝑚𝑒𝑛𝑡 𝑀𝑆 Table at
Treatment (t -1) - CF 𝑇𝑟𝑒𝑎𝑡. 𝐷. 𝑂. 𝐹 𝐸𝑟𝑟𝑜𝑟 𝑀𝑆
𝑟 5% and
σ 𝐴2 𝐴 𝑆𝑆 𝐴 𝑀𝑆 1% level
A (a -1) - CF 𝐴. 𝐷. 𝑂. 𝐹 𝐸𝑟𝑟𝑜𝑟 𝑀𝑆
𝑟𝑏 of
σ 𝐵2 𝐵 𝑆𝑆 𝐵 𝑀𝑆 significa
B (b -1) - CF 𝐵. 𝐷. 𝑂. 𝐹 𝐸𝑟𝑟𝑜𝑟 𝑀𝑆
𝑟𝑎
nce
AXB (a -1)(b -1) Treatment SS-ASS-BSS 𝐴𝑋𝐵 𝑆𝑆 𝐴 𝑋 𝐵 𝑀𝑆
Experimental Total SS- Replication 𝐴𝑋𝐵.𝐷.𝑂.𝐹 𝐸𝑟𝑟𝑜𝑟 𝑀𝑆
(r-1)(ab-1)
Error SS -Treatment SS
𝐸𝑟𝑟𝑜𝑟 𝑆𝑆
𝐸𝑟𝑟𝑜𝑟. 𝐷. 𝑂. 𝐹
Total (rab-1) σ 𝑋 2 - CF
◦ 𝐹𝑐𝑎𝑙 > 𝐹𝑡ab 1% Higly significant **
◦ 𝐹𝑐𝑎𝑙 > 𝐹𝑡ab 5% , 𝐹𝑐𝑎𝑙 ≤ 𝐹𝑡ab 1% Significant *
◦ 𝐹𝑐𝑎𝑙 ≤ 𝐹𝑡ab 5% Non Significant ns

Reference
Page #
15,16
 𝐸𝑟𝑟𝑜𝑟 𝑀𝑆
𝐶𝑉 = × 100
𝐺𝑟𝑎𝑛𝑑 𝑀𝑒𝑎𝑛

Reference
Page # 97
 𝐺𝑇
𝐺𝑟𝑎𝑛𝑑 𝑀𝑒𝑎𝑛 =
𝑟𝑎𝑏
 ‫اسالم وعلیکم‬
‫ورحمتہ ہللا وبرکاتہ!‬