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◊ Wound healing
◊ Treatment of various skin conditions such as leprosy, eczema, psoriasis ◊
Centella
Asiatica
Antioxidant properties

◊ Anti-inflammatory properties

◊ Anti- ageing
◊ Collagen booster

History It was used as one of

the earliest treat-
ments for leprosy in
the UK, while doctors
in France used the
herb to treat every-
thing from dysentery
to infertility.
HISTORY

Centella asiatica has been referred in the French Pharmacopoeia ed. 1884.
Names
Despite its long history of traditional use, Centella appeared in the Codex in
» Centella Asiatica, Gotu Kola, mandukparni, Indian pennywort, jalbrahmi, Tiger 1884 and the first dry extract was not created until 1941. Three years
grass. In China, Gotu kola is known as “miracle elixirs of life” over 2000 years after its triterpenoid molecules were isolated by the French scientist, P.
ago.
Boiteau.

Origin The British Herbal Pharmacopoeia reports the systemic use of the aerial
part of Centella asiatica in rheumatic condition and cutaneous affections
» Madagascar, South Africa, high altitude areas and parts of India. Wild harvest. and the topical application for indolent wounds, leprous ulcers (British
Herbal Pharmacopoeia 1983).
Part Used

» Leaves Folklore and Myths

Use
» In India - Centella asiatica is valued as an ethno medicine as well as in Ayurve-
da and Unani, the traditional Indian medicinal systems for thousands of years
Centella got the nick name tiger’s herb because injured tigers often rub
against it to heal their wounds.
The Tai-Chi Chuan master Li Ching -Yun purportedly lived to an advanced
age of over 200 years old, due in part to his use of Gotu Kola and other
Chinese herbs.

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» MADECASOSSIDE & TERMINOLOSIDE

Centella The mixture of madecassoside, of terminoloside and of asiaticoside extracted
from the parts of Centella asiatica that are above ground can be used in a cos-
Asiatica Ethical practices for
Botanical Sourcing
300 metric tonnes
per Month
metic composition for preventing and delaying premature aging of the skin.

» GENINS
Manufacturing Process These are used in pharmaceuticals due to their antimicrobial activities and
protective role against attempted pathogen infections.

Centella asiatica
manufacturing process
» TECA
Centella Hair oil Triterpenic fractions, the primary constituents of Centella asiatica, produce a
wide range of preventive and therapeutic effects. The modulation of collagen
production and deposition in wound healing is of primary importance. TECA is
Triterpenes
also used to treat several microcirculatory problems, inflammatory skin condi-
Crude Asiaticoside Crude Genins
Glycoside tions (leprosy, varicose ulcers, eczema, atopic dermatitis, psoriasis)

» TRITERPENES
Purified Asiatic Purified Purified Genins Crude Triterpenes are compounds of natural origin, which have numerously biologi-
acid NLT 95% Asiaticoside NLT 95% NLT 95% Madecassoside
cal activities: anti-cancer properties, anti-inflammatory, anti-oxidative, anti-vi-
ral, anti-bacterial and anti-fungal.

+ MT + AC Madecassoside
» FLAVONOIDS
Purity ≥70% Purity ≥ 95%
The flavonoids are shown to have anti-oxidative activity, free radical scaveng-
TECA NLT 95% ing capacity, anti-inflammatory activities

» POLYPHENOLS
The biological properties of polyphenols
include anticancer, antioxidant and anti-in-
Major Molecules
flammatory effects. Polyphenols posses
PRESENT IN CENTELLA ASIATICA anti-microbial and anti-cariogenic properties
and is an important source as anti-infective
» ASIATICOSIDE agents against antibiotic-resistant human
Asiaticoside is a major triterpenoid component derived from Centella asiati- pathogens
ca (L.) and widely used in antioxidant, antiinflammatory, immunomodulato-
ry, and wound healing applications.

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Centella CLINICAL
Asiatica STUDY
Ethical practices for 300 metric tonnes
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Studies on C. Asiatica extracts have reported its potential as an antioxidant, anti-

microbial agent, agent of collagen synthesis and even as a wound healer. Most
studies report asiaticoside as the active constituent producing the said effect. In
ASIATICOSIDE CHARACTERISTICS various wound healing models, topical application (0.2%-0.4%),of asiaticoside has
been shown to increase hydroxyproline content, improve tensile strength,
increase collagen synthesis and remodeling of the collagen matrix, promote epi-
INCI Name thelialization, stimulate glycosaminoglycan synthesis and elevate anti-Oxidant
levels
Asiaticoside

Nature

Extract (Asiaticoside NLT 95%) Ref: (shukla et al., 1999a; shukla et al., 1999b;
somboonwong Et al., 2012). (taemchuay et
Usage level
al., 2009; hashim et al., 2011;Idrus et al., 2012).
0.1 - 1%

Form Ref: (Saad and Said, 2011).

White - cream colour powder

Solubility
In the present study, the wound heal-
organic solvents like ethanol. Insoluble in water
ing potential of Asiaticoside was deter-
Preservative mined using in vitro scratch assay and
in vivo circular wound excision model.
None The basic wound healing process
involves proliferation, migration and
Advantages functioning of fibroblasts and keratino-
Skin conditioning, skin firming, wound healing, Anti-oxidant cytes. Therefore, for the in vitro study,
human dermal fibroblast (HDF) and
human keratinocyte (HaCaT) cell lines
were deemed suitable for use.
The in vivo study conducted afterwards
confirmed the in vitro results, consider-
ing some compounds that show prom-
ising activity in vitro may be metabo-
lized into inactive metabolites in vivo.
However, this does not indicate that
the in vivo test is more significant com-
pared to the in vitro test. The latter is
more suitable for quick, inexpensive
screening tests. While both tests have
different roles in research, they actually
complement each other

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Ethical practices for 300 metric tonnes
Botanical Sourcing per Month
Asiaticoside, a constituent in CA, has
been reported to possess wound
healing activity by increasing colla-
gen formation and angiogenesis.
Apart from showing a stimulation of
the collagen synthesis in different
cell types, the asiaticoside
Ref : Rosen H, Blumenthal A, McCallum J. Effect of asiaticoside on wound heal-
ing 1967;125:279-80.
07
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were shown to increase the tensile
strength of the newly formed skin,
furthering the healing of the
wounds. Also, it was shown to inhibit
the inflammatory process which
may provoke hypertrophy in scars
and improves the capillary permea-
bility
Centella Asia
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PROCESS OF
SKIN REPAIR
Asiaticoside, a constituent in CA, has been
reported to possess wound healing activity
by increasing collagen formation and angio-
genesis. Apart from showing a stimulation
of the collagen synthesis in different cell
types, the asiaticoside were shown to
increase the tensile strength of the newly
formed skin, furthering the healing of the
wounds. Also, it was shown to inhibit the
inflammatory process which may provoke
hypertrophy in scars and improves the capil-
lary permeability
Reference : Triterpene Composition and Bioactivities of Centella asiatica , Puziah
Hashim 1,*, Hamidah Sidek 2, Mohd Helme M. Helan 2, Aidawati Sabery 2, Uma Devi Palani-
samy 3 and Mohd Ilham 4
ntella Asiatica 08
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Centella MADECASOSSIDE &

Asiatica TERMINOLOSIDE
Ethical practices for 300 metric tonnes
Botanical Sourcing per Month

If you're starting to notice age spots, deep

wrinkles or a bit of sagging skin on your
face, your seeing the effect of structural
MADECASSOSIDE & TERMINOLOSIDE
changes associated with skin damage.
CHARACTERISTICS
Damaged skin is something all of us expe-
INCI Name rience, but it doesn't have to stay that way.

Madecasosside & Terminoloside (MT) Madecassoside & Terminoloside(MT), also

classed as a triterpenine, stimulates pro-
Nature
duction of type III collagen. While asiatico-
Extract (MT NLT 90%) side handles the wound-healing side of
things, MT separates itself from the pack
Usage level with its anti-inflammatory properties,
making it a popular ingredient for
0.1 - 0.5%
acne-prone skin.
Form
In studies, MT has demonstrated skin heal-
White - cream colour powder MT has demonstrated
ing as well as anti-aging properties in skin.
Solubility some benefit in the treat- It works in several ways including
ment of psoriasis, most
Soluble in water and other organic solvents Inflammation control
likely due to its anti-inflam-
Preservative matory properties.
Stimulating the synthesis of collagen III,
None integral components of the skin.
The aim of this study was to
Advantages test the efficacy of C. asiati- Reducing the activity of MMPs - matrix
ca extract containing metallo proteins - or enzymes that play a
Anti - ageing, Anti- flammatory, skin revitaliser,
cream versus a placebo role in the breakdown of the skin’s matrix
skin -smoothening, skin repair
or framework.
cream on 20 volunteers.

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Volunteers were selected according to

previously agreed inclusion criteria
Basal measurements as well as final
(age 40-70 years) . Each volunteer
evaluations were carried out at the
represented their own placebo, apply-
beginning and at the end of the Centella
ing either the placebo or the test
emulsion on each half of the face (ran-
study.The formulation containing
asiatica extract at 0.5% was applied,
C. Asiatica Ethical practices for 300 metric tonnes
Botanical Sourcing per Month
domly selected). The minimal erythe-
together with the control emulsion,
mal dose on the forearm was also
on one side of the faces of 20 volun-
assessed on 20 volunteers aged 55±9
teers over 6 weeks. Collagen redensifi-
years. For the anti-wrinkle evaluations,
the same volunteers were required to
cation, evaluated by Siascope, was GENINS
apply the tested emulsion and the
observed in 70% of the tested volun- CHARACTERISTICS
teers (P<0.05), and a significant
placebo emulsion (randomly select-
decrease in wrinkle depth and
ed) each on one half of the face for 6 INCI Name
volume was observed.
weeks.
Asiatic acid and Madecassic acid

The half of the face treated with the centella-containing formulation also Nature
showed improved biomechanical elasticity of the skin (evaluated by
Extract (sum of Asiatic acid and Madecassic acid NLT 90%)
Cutometer, parameter R5, Figure 1A and 1B) and skin firmness (evaluated
by Cutometer parameter R7) by 29% and 17%, respectively (P<0.05) (Figure
Usage level
1A and 1B).
0.1 - 0.5%

Form

White to off-white

Solubility

Soluble in alcohol, insoluble in water

Preservative

None

Advantages

Natural antibiotic, antibacterial properties, for anti-acne

products

Ref :Giada Maramaldi,Stefano Togni, Federico Franceschi,Elian Lati Indena SpA,

Milan, Italy; Laboratoire BIO-EC, Longjumeau, France

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GENINS GENINS
(Asiatic Acid & Madecassic Acid) Reports

Asiatic acid is an isolated and purified triterpenic acid of Centella asiatica

Asiatic Acid (30%) leaves. It is recommended for skin care, anti-aging and replumping thanks to
Strengthens the skin barrier, soothes irritated skin, antioxidant, acts as its proven efficacy on GAGs((glycosaminoglycans), on proteoglycan release,
anti-photoaging agent on collagen synthesis activation and on antimicrobial activity.

Madecassic Acid (40%)

Soothes, makes pimples and pigment shifts fade, has a gentle effect on
acne and its long-term signs.

Sun Protection

UVB

UVA

Photoaging is the premature aging of the skin caused by repeated exposure to

ultraviolet radiation (UV) primarily from the sun but also from artificial UV sourc-
es. Photoaging differs from chronological aging: the damaging effects of UV rays
- from the sun or artificial tanning sources - alter normal skin structures.

Research has indicated that Asiatic Acid and Madecassic acid can be used to
In an in-vitro (made in the lab) studies show that Asiatic Acid also stimulates
treat photo-aging skin. This is largely due to the enhancing effect that it has on
GAGs (glycosaminoglycans - polysaccharides that are part of the liquidy stuff
collagen.
between our skin cells) production as well as collagen I synthesis.

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TECA -TITRATED EXTRACT OF

Centella CENTELLA ASIATICA
Asiatica
Ethical practices for 300 metric tonnes
Botanical Sourcing per Month

TECA comprises of asiatic acid, madecassic acid, asiaticoside and madecas-

soside, and this mixture is commercially marketed as a titrated extract of
Centella asiatica (TECA).

It is used as an anti-microbial, anti-oxidative and anticancer agent, as well as a

TECA -TITRATED EXTRACT OF CENTELLA ASIATICA
therapeutic agent in the various processes of wound healing, such as coag-
CHARACTERISTICS ulation, inflammation, cell migration and proliferation as well as scar forma-
tion and remodeling.
INCI Name

Asiaticoside - Asiatic acid - Madecassic acid

Nature

Extract (sum of Asiatic acid and Madecassic acid NLT 90%)

Usage level CLINICAL

0.1 - 1%
TRIALS
Form
Trofolastin has been used in a study Their results demonstrated that 56%
White to off-white for the prevention of Striae Distensae
of subjects in the placebo group com-
(SD) and also contains the active
pared to 34% in the treatment group
Solubility ingredients; TECA (Centella asiatica), developed SD. Additionally, in women
tocopherol and collagen-elastin who had a history of SD during puber-
Insoluble in water, soluble in methanol
hydrolysates. It is suggested that TECA ty, the treatment cream gave 100%
Preservative stimulates fibroblasts and has an prevention of SD, compared to 89% in
antagonistic effect against glucocorti- the placebo group.
None
coids. A randomized controlled trial
compared the treatment cream to a
Advantages
placebo in 80 subjects.
Natural antibiotic, antibacterial properties, for anti-acne
products, Anti-stretch marks, protection against UV rays A number of products contain the active ingredient TECA (Centella Asiatica)
which is suggested to stimulate cell production. Liforma® Stretch Mark Day Gel
and Night Cream is a moisturizing cream which contains TECA (Centella Asiati-
ca) and claims to prevent Striae Distensae (SD). A product comparative study
was undertaken with 25 women, who completed a questionnaire. They reported
that Liforma® scored the highest in all outcomes.

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Reference : Topical management of striae distensae (stretch marks): prevention and thera-
py of striae rubrae and albae S. Ud-Din,1'2 D. McGeorge,3 A. Bayat1'2'*
OUR’S CENTELLA EXTRACTS
1. Plastic and Reconstructive Surgery Research, Manchester Institute of Biotechnology,
University of Manchester, Manchester, UK
VS. OTHER CENTELLA EXTRACTS
2. Centre for Dermatology, Institute of Inflammation and Repair, Faculty of Medical
and Human Sciences, University of Manchester, Manchester Academic Health The raw material is from Madagascar with following benefits
Science Centre, Manchester, UK
More active content in the material
3. Centre for Dermatology, Institute of Inflammation and Repair, Faculty of Medical Wild cultivated
and Human Sciences, University of Manchester, Manchester Academic Health Wildly cultivated material is more beneficial in terms of cultivated
Science Centre, Manchester, UK Centella asiatica as in the later process, there is loss/reduction of
active content

The botanical properties of the product varies from place to place and is
more location specific

Ethically sourced : UEBT/Nagoya

Complete traceability

Precisely purified molecules in our extracts

Involved in extensively Sustainably sourced project for this ingredient

We are the largest manufacturer of this in India and sell all over the world
predominantly in Europe and South Korea to large well established
brands.

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