VELAMMAL NEET & IIT ACADEMY

1. A plant cell has potential to develop into full plant. This property of the plant
cell is called ________ .
(a) tissue culture (b) totipotency (c) pleuripotency (d) gene cloning
2. An explant is a
(a) dead plant. (b) part of the plant.
(c) part of the plant used in tissue culture.
(d) part of the plant that expresses a specific gene.
3. The technique of obtaining large number of plantlets by tissue culture method is
called --------.
(a) micropropagation (b) micropropagation
(c) plantlet culture (d) organ culture
4. Protoplast of two different species are fused in _______.
(a) micropropagation (b) somatic hybridization
(c) clonal propagation (d) organography
5. Which of the following is incorrectly matched?
(a) Explant – Excised plant part used for callus formation
(b) Cytokinins – Root initiation in callus
(c) Somatic embryo – Embryo produced from a vegetative cells
(d) Anther culture – Haploid plants
In the following questions, a statement of Assertion is followed by a statement
of Reason.
(a) Assertion and Reason are true and the Reason is the correct explanation of
the Assertion.
(b) Assertion and Reason are true but the Reason is not the correct
explanation of the Assertion.
(c) Assertion is true but Reason is false. (d) Assertion and Reason are false.
6. Assertion: Somatic embryos can be induced from any cell in plant tissue culture.
Reason: Any living plant cell is capable of differentiating into somatic
embryos.
7. Assertion: An important technique of genetic engineering is protoplast culture.
Reason: It results in the production of genetically modified crops.
8. Assertion: Meristem tissue culture can produced virus free plants from virus
infected plants.
Reason: In this technique the growth of the virus is inhibited during the growth
of the host tissue.
9. Assertion: A major advantage of tissue culture is protoplast fusion.
Reason: A hybrid is formed by the fusion of naked protoplasts of two plants.
10. Select the correct chronological order of the events occuring during callus
culture
(a) Callus  Cell division  Explant  Addition of cytokinin  Cells acquire
meristematic property.
(b) Explant  Callus  Cell division  Addition of cytokinin Cells acquire
meristematic property.
(c) Explant  Cell division  Callus  Addition of cytokinin  Cells acquire
meristematic property.
(d) Callus  Explant  Cell division  Addition of cytokinin  Cells acquire
meristematic property.
11. In order to obtain virus-free plants through tissue culture the best method is
(a) meristem culture (b) protoplast culture
(c) embryo rescue (d) anther culture
12. Which of the following pair of hormones are required for a callus to
differentiate?
(a) Auxin and cytokinin (b) Auxin and ethylene
(c) Auxin and abscisic acid (d) Cytokinin and gibberellin
13. In tissue culture medium, the embryoids formed from pollen grains is due to
(a) cellular totipotency (b) organogenesis
(c) double fertilization (d) test-tube culture
14. Totipotency refers to the
(a) capacity to generate genetically identical plants.
(b) capacity to generate a whole plant from any plant cell explant.
(c) capacity to generate hybrid protoplasts.
(d) recovery of healthy plants from diseased plants.
15. Which of the following induces fusion of protoplasts ?
a) IAA & Gibberellins b) Polythene glycol & Sodium nitrate
c) IAA & Kinetin d) NaCl & KCl
16. Virus free plants can be obtained from
a) Bordeaux mixture b) antiviral treatment
c) Root hair d) Shoot tip
17.

20.
22. Micropropagation is
1) Propagation of microbes in vitro 2) Propagation of plant in vitro
3) Growing plants on smaller scale 4) Propagation of virus in vitro
23. To obtain virus – free healthy plants from a diseased one by tissue culture
technique, which part / parts of the diseased plant will be taken?
1) Apical meristem only 2) Palisade parenchyma
3) Both apical and axillary meristems 4) Epidermis only
24. Fusion between two naked protoplasts is known as
1) Somatic hybridisation 2) Germinal hybridisation
3) Parasexual hybridisation 4) Both 1 & 3
25. In anther culture, anthers of some plants are used for regeneration of new
plants. These anthers can be referred as
1) Protoplast 2) haploid plant 3) Explant 4) Cybrid
26. The capacity to generate a whole plant from any cell/explant is called :-
1) Somatic embryogenesis 2) Tissue culture
3) Totipotency 4) Micropropagation
27. Somatic hybridization is :-
1) Fusion of male and female nucleus in a zygote 2) Vegetation reproduction
3) Fusion of two protoplast 4) Sexual reproduction
28. Which one of the following is a case of wrong matching ?
1) Micropropagation –Invitro production of plants in large numbers
2) Callus – Unorganised mass of cells produced in tissue culture
3) Somatic hybridization – Fusion of two diverse cells
4) Vector DNA – Site for t-RNA synthesis
29. Embryo culture is mostly used for :-
1) Establishing suspension culture 2) Recovery of interspecific hybrids
3) Somatic hybridization 4) Haploid plant production
30. The method of producing thousands of clone of a plants through tissue culture
is called:
1) Totipotency 2) Micropropagation
3) Somatic hybridization 4) Biofortification
31. ‘Pomato’ is developed by which tissue culture method:
1) Meristem culture 2) Embryo culture
3) Anther culture 4) Somatic hybridization
32. Somaclones are :-
1) Somatic hybrids 2) Genetically identical to the original plant
3) Used to recover disease free plant 4) Sterile plants
33. Cellular totipotency is demonstrated by :
1) Only gymnosperm cells 2) All plant cells
3) All eukaryotic cells 4) Only bacterial cells
34. The technique of obtaining large number of plant lets by tissue culture method
is called
1) Organ culture 2) somatic hybridisation
 3) Macropropagation 4) Plantlet culture
35. Somaclones are obtained by
1) Genetic engineering 2) Tissue culture
3) Plant breeding 4) Irradiation genetic engineering
36. Which one of the following is a case of wrong matching ?
1) Micropropagation – In vitro production of plants in large numbers
2) Callus – Unorganised mass of cells produced in tissue culture
3) Somatic hybridization – Fusion of two diverse cells
4) Vector DNA – site for t-RNA synthesis
37. Which part would be most suitable for raising virus free plants for
micropropagation?
1) Meristem 2) Node 3) Bark 4) Vascular tissue
38. Somaclonal variations are
(1) Caused by gamma rays (2) Produced during sexual reproduction
(3) Produced by chemical mutagens (4) Produced during tissue culture
39. Select the incorrect option (w.r.t. tissue culture)
(1) Genetically identical – Somaclones
(2) Capacity to generate a whole plant – Totipotency
(3) Plant cell without cell wall – Protoplast
(4) Virus free part of plant – Root
40. Somatic hybridisation is accomplished by
(1) Grafting between two varieties
(2) Fusion of two protoplast of different varieties of plants
(3) Fusion of two cytoplast of different varieties of plants
(4) Fusion of two protoplast of same varieties of plants
41. Choose correct option w.r.t. tissue culture
(1) The whole plant can be regenerated from explant
(2) Only apical meristem part of plant can grow in test tube under sterile
conditions
(3) Totipotency related to all living organisms
(4) Sterilisation is not a pre-requisite
42. Culturing meristem of banana, sugarcane and potato is primarily significant to
produce
(1) Somatic hybrids (2) Virus free plants
(3) Specific meristematic tissue (4) Hybrid vigour

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