MICROBIOLOGY LABORATORY EXERCISES

PHYSICAL METHODS OF INACTIVATION OF MICROORGANISMS

NAME _____________________________________________ SEC./GROUP NO. _____ RATING _____

Record the results in the following table as follows: (2 points)

(+) clear (++) slightly turbid (+++) very turbid

Result observed
B. subtilis E. coli

Autoclaving

Boiling for 30 minutes

Heating at 60OC for 30 minutes

Hot air oven (1800C for 2 hours)

Control

Answer briefly the following questions in the space provided. (1 point each)

1. What can explain the difference in results for E. coli and B. subtilis?

2. What is the mechanism of action of fractional sterilization?

3. What is the main use of lyophilization?

Corrected by: ______________________________ Date: ___________