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Smart Innovation, Systems and Technologies 207
Proceedings of the
7th Brazilian
Technology Symposium
(BTSym’21)
Emerging Trends in Human Smart and
Sustainable Future of Cities (Volume 1)
123
Smart Innovation, Systems and Technologies
Volume 207
Series Editors
Robert J. Howlett, Bournemouth University and KES International,
Shoreham-by-Sea, UK
Lakhmi C. Jain, KES International, Shoreham-by-Sea, UK
The Smart Innovation, Systems and Technologies book series encompasses the
topics of knowledge, intelligence, innovation and sustainability. The aim of the
series is to make available a platform for the publication of books on all aspects of
single and multi-disciplinary research on these themes in order to make the latest
results available in a readily-accessible form. Volumes on interdisciplinary research
combining two or more of these areas is particularly sought.
The series covers systems and paradigms that employ knowledge and intelligence
in a broad sense. Its scope is systems having embedded knowledge and intelligence,
which may be applied to the solution of world problems in industry, the environment
and the community. It also focusses on the knowledge-transfer methodologies and
innovation strategies employed to make this happen effectively. The combination of
intelligent systems tools and a broad range of applications introduces a need for a
synergy of disciplines from science, technology, business and the humanities. The
series will include conference proceedings, edited collections, monographs, hand-
books, reference books, and other relevant types of book in areas of science and
technology where smart systems and technologies can offer innovative solutions.
High quality content is an essential feature for all book proposals accepted for the
series. It is expected that editors of all accepted volumes will ensure that
contributions are subjected to an appropriate level of reviewing process and adhere
to KES quality principles.
Indexed by SCOPUS, EI Compendex, INSPEC, WTI Frankfurt eG, zbMATH,
Japanese Science and Technology Agency (JST), SCImago, DBLP.
All books published in the series are submitted for consideration in Web of Science.
Editors
123
Editors
Yuzo Iano Osamu Saotome
Faculty of Electrical Divisão de Engenharia Eletrônica
and Computer Engineering Instituto Tecnológico de Aeronáutica
Unicamp São José dos Campos, São Paulo, Brazil
Campinas, São Paulo, Brazil
Claudia Cotrim Pezzuto
Guillermo Leopoldo Kemper Vásquez Pontifícia Universidade Católica de
Universidad Peruana de Ciencias Aplicadas Campinas
Santiago de Surco, Lima, Peru Campinas, Brazil
This Springer imprint is published by the registered company Springer Nature Switzerland AG
The registered company address is: Gewerbestrasse 11, 6330 Cham, Switzerland
Foreword
With great satisfaction, I write this Foreword for the Proceedings of the 7th
Brazilian Technology Symposium–Emerging Trends and Challenges in
Technology (BTSym’21), held virtually, for the second time, at the PUC Campinas
University, Brazil, in November 2021, and the UNAPUNO University, Peru, in
January 2022. This event is in its sixth edition and has consolidated to become an
excellent opportunity for researchers, professors, and students to present and dis-
cuss the results of their research works.
In the 2021 edition, the BTSym activities could not be accomplished in person
due to the COVID-19 pandemic. However, the event has been characterized since
its first edition by the broad scope of the areas exposed and, within a virtual
environment, it was possible to expand our network of researchers and encourage
them to expose their papers, which deal with current and priority topics for
Brazilian and world technological development, including subjects related to the
various branches of innovation in industrial processes, robotics, telecommunica-
tions, buildings, urban infrastructure, product development, and biomedicines.
Events such as BTSym are an essential part of the research and innovation
process. Firstly, these events contribute to the promotion of research activities,
which are key to a country’s technological development. The dissemination of
research results, as promoted by BTSym, contributes to the transformation of
research findings into technological innovation. In addition, these events facilitate
the sharing of findings, leading eventually to the formulation of research networks,
which accelerate the achievement of new results. Therefore, I would like to con-
gratulate the BTSym General Chair, Prof. Dr. Yuzo Iano, and his group of col-
laborators for the important initiative of organizing the BTSym’21 and for
providing the opportunity for authors to present their work to a wide audience
through this publication. Finally, I congratulate the authors for the high-quality
work presented in these proceedings.
v
Preface
This book contains the Proceedings of the 7th Brazilian Technology Symposium–
Emerging Trends and Challenges in Technology, held in Brazil in November 2021
and Peru in January 2022.
The Brazilian Technology Symposium is an excellent forum for presentations
and discussions of the latest results of projects and development research in several
areas of knowledge, in scientific and technological scope, including smart designs,
sustainability, inclusion, future technologies, architecture and urbanism, computer
science, information science, industrial design, aerospace engineering, agricultural
engineering, biomedical engineering, civil engineering, control and automation
engineering, production engineering, electrical engineering, chemical engineering,
and probability and statistics.
This event seeks to bring together researchers, students, and professionals from
the industrial and academic sectors, seeking to create and/or strengthen the linkages
between issues of joint interest. Participants were invited to submit research papers
with methodologies and results achieved in scientific level research projects,
completion of course work for graduation, dissertations, and theses.
The 67 full chapters accepted for this book were selected from 208 submissions,
and, in each case, the authors were guided by an experienced researcher with a
rigorous peer-view process. Among the main topics covered in this book, we can
highlight manufacturing processes, lean manufacturing, industrial costing models,
sustainability and productivity, circular economy, workplace safety, control sys-
tems, Internet of Things, cyber-physical systems, transportation management sys-
tem (TMS), logistic services analysis, digital supply chain, socio-economic impacts
of technologies 4.0, robotics applications, artificial neural networks, big data, deep
learning, computational vision, cybersecurity, soft-computing methodologies,
technologies applied to cities development, smart cities, energy sustainability,
building information modeling (BIM), environment analysis, technologies applied
to health, biomedical innovations, socio-economic impacts of COVID-19, tech-
nologies applied to education, academic development, civil aviation studies, and
much more.
vii
viii Preface
We hope you enjoy and take advantage of this book and feel motivated to submit
your papers, in the future, to Brazilian Technology Symposium.
Best wishes,
Our appreciation goes to a lot of colleagues and friends who assisted in the
development of this book, Proceedings of the 7th Brazilian Technology
Symposium–Emerging Trends and Challenges in Technology (BTSym’21).
First of all, I would like to thank all the members of the organizing and executive
committee for the commitment throughout the year. Several meetings were held,
and many challenges were overcome for the accomplishment of the BTSym 2021.
Also, and with great merit, I would like to thank all the scientific and academic
committee and technical reviewers committee members for their excellent work,
which was essential to ensure the quality of our peer-review process, collaborating
with the visibility and technical quality of the BTSym 2021.
The Brazilian Technology Symposium is an event created by the Laboratory of
Visual Communications of the Faculty of Electrical and Computer Engineering
of the University of Campinas (UNICAMP). In this way, I would like to thank the
PUC Campinas and UNAPUNO Universities, especially for supporting and hosting
the BTSym’21 and BTSym’21 Satellite, respectively, which was fundamental for
the successful accomplishment of the events.
Finally, on behalf of Prof. Yuzo Iano, the General Chair of the Brazilian
Technology Symposium, I thank all the authors for their participation in the
BTSym’21; we sincerely hope to have provided a very useful and enriching
experience in the personal and professional lives of everyone.
Best wishes,
ix
Contributors
Organizing Committee
xi
xii Contributors
Executive Committee
Abel Dueñas Rodríguez LCV/DECOM/FEEC/UNICAMP
(Midia Chair)
Airton Vegette LCV/DECOM/FEEC/UNICAMP
(Institutional
Relationship Chair)
Angélica F. G. LCV/DECOM/FEEC/UNICAMP
(Institutional
Relationship Chair)
Daniel B. Katze LCV/DECOM/FEEC/UNICAMP
(Institutional
Relationship Chair)
Daniellle Thiago Ferreira LCV/DECOM/FEEC/UNICAMP
(Editorial
Committee Chair)
Elizangela Santos Souza LCV/DECOM/FEEC/UNICAMP
(Editorial
Committee Chair)
Gabriel Caumo Vaz LCV/DECOM/FEEC/UNICAMP
(Institutional
Relationship Chair)
Jennifer Chuin Lee LCV/DECOM/FEEC/UNICAMP
(Designer Chair)
João Carlos Gabriel Universidade Presbiteriana Mackenzie
(Campinas-Vice-Associate-
General Chair BTSym)
Leticia Cursi LCV/DECOM/FEEC/UNICAMP
(Institutional
Relationship Chair)
Lucas Alves LCV/DECOM/FEEC/UNICAMP
(Institutional
Relationship Chair)
Luiz Vicente F. de Mello Universidade Presbiteriana Mackenzie
Filho (Campinas-Vice-
Associate-General Chair
BTSym)
Mariana Melo LCV/DECOM/FEEC/UNICAMP
(Institutional
Relationship Chair)
Paulo Roberto dos Santos UniMetrocamp
(Vice-Associate-General
Chair BTSym)
Contributors xiii
xvii
xviii Contents
1 Introduction
Among different modes of exercise in busy modern life, cycling and ergometric tread-
milling activities are the most common indoor aerobic exercises. Treadmills are very
tall and occupy more space. Moreover, this makes people lazy, relying on machines for
doing their workouts [1–3]. We feel that this bicycle is the first of its kind that works with
human strength taking up less space. We combined both the transportation and physical
fitness aspects in one single vehicle. In this vehicle, there are no seating arrangements;
instead, we must stand over the platform and perform the operation.
This vehicle is based on a mechanism, which drives the vehicle forward, and it would
be an excellent support for improving physical fitness. The main thing that boosts this
vehicle is that it is completely fabricated from scrap materials, so that, we can re-use the
scrap for some useful work, and this makes this vehicle economically friendly [4–7].
The motivation for this project originated from conventional kick bikes, which are used
in day-to-day life. The traditional kick scooters consist of two wheels, the front wheel
attached with the handlebar and the steering mechanism. The back wheel is connected
to the center deck, which is free to rotate. The user must stand over the deck and uses his
leg to kick the ground; due to force created by kicking, the scooter moves in the forward
direction. The deck is an area or platform where the rider stands over it to operate the
vehicle [8–11].
The principle used behind this kick scooter is Newton’s third law of motion. As per
Newton’s third law of action, “For each activity, there is an equivalent and inverse
response.” These two powers are called activity and response powers. The detailed view
of the existing kick scooter is shown in Fig. 1.
A kick bicycle or push bicycle is a human-operated vehicle with handlebars and wheels,
controlled by a driver pushing off the ground. Most of the bikes today are made of hard
metals to avoid wear and tear. Toddler bicycles are made of thick plastic. Elite bikes were
made for grown-up people, and they look like the old “penny-farthing.” Mechanized
bikes, generally controlled by gas motors or electric engines, are self-pushed kick bikes
equipped for rates of around 30 km/h (19 mph).
Design and Fabrication of Human-Powered Vehicle 3
2.4 Kick ’N Go
In 1974, the Kick ‘n Go was introduced by Honda. This bike is driven by moving a
pedal connected to the propelling mechanism. While it was considered, by all accounts,
as much exertion to “kick” like in an ordinary bike, the curiosity for the Kick ’n Go got
on, and it became mainstream. It is depicted in Fig. 3.
Denis Johnson built a similar vehicle in the 19th century. The invention of the kick
bike in Finland, in 1994, altered the perception of scooters. The Kick bike features a
massive standard-size bicycle with a front wheel and a smaller rear wheel. That config-
uration permits a much quicker ride. Since 2001, the Foot Bike Euro Cup has been used
commercially, which is shown in Fig. 4.
Wim Ouboter, from Micro Mobility Systems, in Switzerland, fabricated a foldable alu-
minum motorcycle with inline skate wheels in 1996. The bike is marketed as a “Miniature
Scale Skate Scooter” and “JDBUG/JDRAZOR MS-130A” (Fig. 5). After the Razor was
introduced in Japan, in 1999, many young people in Tokyo began to use it as a small trans-
port medium, and it quickly became a popular trend around the world and also became a
popular children’s toy. Newly designed Foldable kick bikes designed for adults typically
have more robust parts: they are designed with larger decks, hand brakes, and larger
wheels for smoother transportation rather than lighter weight and portability. The Xootr
Street, which has 180 mm wheels and a maximum weight of 136 kg. Go-Ped is a type
of bicycle. Know-Ped bikes have 6-in. wheels with strong elastic tires and a maximum
load of 181 kg, depending on the model. Kick Ped from NYC Wheels, which is devoid
of every single delicate component, for example, a handbrake, and is replaced with a
back spoon brake to make the kick bike reliable and resistant [12, 13].
Design and Fabrication of Human-Powered Vehicle 5
3 Problem Identification
In recent times, the increase in obesity is the main problem among us. As a result,
exercise is recommended for health promotion and prevention of many cardiovascular
diseases, as well as for rehabilitation following an illness episode. Aerobic exercises,
which include running, jogging, walking, cycling, and others, are among the activities
that are appropriate for those purposes.
So, we introduce our vehicle, which is powered by a human load, and it does not
need any fossil or petroleum fuels. Using this vehicle for a few minutes gives us the same
workout we get at the gym. By using this vehicle, we can obtain both physical fitness
and transportation aspects. Thereby it is an economical, ecological, and useful product.
This vehicle runs on human load so that the cost of petroleum fuels is eliminated, so
that, it does not produce any emissions to the atmosphere and is completely safe for the
ecology [14–17].
4.1 2D Model
The two-dimensional model is an essential perspective on any article where the item
is anticipated. The 2D model is made utilizing two-axis (X and Y). The fundamental
6 Y. Thiagarajan et al.
4.2 3D Model
The three-dimensional model shows the standard view of the item as it is. It is precious
to build up the article in a reasonable human view. To make a 3D model, we prefer three
axes (x, y, and z). There is much programming for creating a 3D model, as indicated by
our determinations. Some products that are utilized to make the 3D model are AutoCAD,
Pro-E, CREO, and Solid Works [10–13].
AutoCAD is a useful workbench for design and drafting programs. Autodesk cre-
ated and marketed it. AutoCAD is used by architects, project managers, engineers,
graphic designers, town planners, and many other professionals in a variety of industrial
applications. AutoCAD Mechanical 2018 version is utilized for a better design.
Here we have used the “Ansys 15.0” software to get the results and calculations. In
this model, we have two frames and wheels with a handlebar. We have analyzed it by
using two different materials like structural steel and aluminum alloy. Here the complete
vehicle without any mechanism has been tested. The tilted 3D view of the vehicle is
illustrated in Fig. 9, using Ansys Software.
8 Y. Thiagarajan et al.
5 Results
The deformation of the vehicle when the load acts on it is analyzed, and the following
strains are calculated for 75 kg and 150 kg.
Total distortion is the overall deformation of the vehicle when the pressure is applied.
The total deformation of the vehicle is tested for 75 kg, as depicted in Fig. 10. The red
portion indicates the maximum deformation area of the vehicle when the load acts on
it. The blue part shows the minimum deformation area of the vehicle when the pressure
works on it.
The total deformation of the vehicle is tested for a load of 150 kg. The red portion
indicates the maximum deformation area of the vehicle when the pressure acts on it. The
blue part shows the minimum deformation area of the vehicle when the load works on
it, which is clearly illustrated in Fig. 11.
Design and Fabrication of Human-Powered Vehicle 9
Fig. 11. Total deformation of the vehicle tested for 150 kg load
The directional deformation of the vehicle is tested for the load of 75 kg. Directional
deformation is the deformation of the vehicle in a particular axis, say x-axis, when the
pressure is applied. The red portion indicates the maximum deformation area of the
vehicle when the load acts on it. The blue part shows the minimum deformation area of
the vehicle when the pressure works on it, which illustrated in Fig. 12.
10 Y. Thiagarajan et al.
Fig. 13. Directional deformation of the vehicle tested for 150 kg load
for the weight of 75 kg. The red portion indicates the maximum principal stress area of
the vehicle when the pressure acts on it. The blue part indicates the minimum principal
stress area of the vehicle when the load serves on it; these are highlighted in Fig. 14.
Fig. 14. Maximum principal stress of the vehicle tested for 75 kg load
The Maximum principal stress of the vehicle is tested for the load of 150 kg. The red
portion indicates the maximum principal stress area of the vehicle when the load acts on
it. The blue portion indicates the minimum principal stress area of the vehicle when the
load acts on it, which is shown in Fig. 15.
Fig. 15. Maximum principal stress of the vehicle tested for 150 kg load
12 Y. Thiagarajan et al.
By analyzing the vehicle using the “Ansys 15.0” software, we came to know that
the deformation of the vehicle, when the load is applied on it, is minimal. Hence, it can
withstand an average human load, and the vehicle is completely safe when used by an
average human load.
6 Hardware Description
This vehicle “converts a vertical external load (force) into a rotational or twisting force,”
thereby making the vehicle move in the forward direction. The external load is in the form
of reciprocating in nature, which is transferred to the wheels through the combination
of chain drive and pulley drive.
6.1 Working
The body is comprised of two casings that are associated with a typical focal pivot.
Likewise, a spring holds both bodies consistently, and they are moved when there is a
human load on them. Further, the portable front part is associated with chains utilizing
an instrument situated at the other mobile part, which is on two apparatus. Consequently,
when someone hops on it, and the body extends, the chains are extended as well. In this
way, the appliances are returning to move the pulley. Also, when the human’s weight is
expelled from the bicycle, the riggings are taking their underlying position, and to make
it move once more, another jump ought to be made.
For the most part, the specific system transforms the flat move into a turning one.
The last step of the wheels is brought about by a pulley through a belt put on the hub
and arrives at the wheels. Regardless of whether the specific vehicle is a typical bicycle
or not, it is charming and fascinating to ride using it. Figure 16 showcases the basic
2D paint sketch of the personalized vehicle and Fig. 17 is the photographic view of the
Fabricated Hardware model.
7 Conclusion
In this modern era, physical wellness is considered one of the most extreme significances
in everyday life. This bike is designed after continuous assessment and modification so
that it could be commercialized in the market for better use. Our target is to come up
with an innovative concept that would serve the purpose of both the health concern and
transportation.
The design and the idea are brought into reality with few compromises like lack of
funds, time, and fabrication facility in our environment. However, this planned human-
powered vehicle was developed with high accuracy in design. The major highlight is
that it does not require any nonrenewable energy source for its working, thereby eradi-
cating carbon footprint, and it is also user-friendly. By utilizing this vehicle, one can get
physically fit.
The design has been plotted out in ANSYS Version 15. The vehicle’s physical
strength was analyzed by manipulating the design under various load conditions. This
approach is made to have a better understanding of the vehicle. The fabricated bike was
put into test drive with various human weights, and the result showed a perfect remark.
Finally, the creation and the trial have been expertly finished, and the vehicle is ready to
be on board.
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for India. UNEP DTU Partnership (2014)
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nomena investigation of a new nonlinear dynamical system 4D by Gardano’s and Lyapunov’s
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(2014)
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(2012)
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101–106 (2020)
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aware detection in wireless networks. Comput. Mater. Continua 66(2), 2077–2086 (2021)
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environment planning using artificial intelligence techniques. Environ. Impact Assess Rev.
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Identification and Analysis of Bacterial Species
Present in Cow Dung Fed Microbial Fuel Cell
oliveiragomesgabriel@ieee.org, yuzo@unicamp.br,
gabriel_caumo@yahoo.com.br
Abstract. Microbes are the powerhouses of Microbial fuel cells (MFCs). The
present report is the offshoot of research undertaken on the use of cow dung
slurry for electricity generation. Since bacterial consortia are the driving force in
MFCs, bacterial communities present in cow dung slurry and on the biofilm of the
electrodes were screened by Matrix-assisted laser desorption/ionization-time of
flight technique. More than 60 bacterial species and a few unidentified but having
similarity with identified bacterial species were found in the cow dung slurry; only
seven bacterial species were found on biofilm formed on the electrode and involved
in redox reactions. These findings are considered as the route map to achieve
higher electricity using the specific group of bacteria in MFCs. Further, present
findings enlighten the potential opportunity that bacterial species isolated from
the electrode surface can be further cultured individually and used in Bioreactor
to fasten the energy production from cow dung slurry fed Bioreactor.
1 Introduction
Microbial power device or (MFC) arose as one of the sustainable power producing mod-
ule for low power applications. Microbes present in the organic substrates used in MFCs
are the driving force in generating electricity in MFCs. Energy is generated in MFC’s
due to the oxidation of organic substances present in them, and that energy is capitalized
as the new renewable source using MFC. Therefore, the selection of bacterial consor-
tia and optimization of the microbial metabolism to improve electron production have
become the core area of interest in this millennium. Microbes colonized on electrodes
deployed in MFC acquire the required energy for cellular growth from the oxidation of
the complex organic matters and release electrons [1].
It has been well established that matter supply and blend of microorganism com-
munity are the key factors that are thought of as core inputs of MFC. Eventually, the
search for potential substrates naturally bestowed with more diverse bacterial commu-
nities has been taken up in the recent past by many researchers. For direct bioelectricity
generation in MFC, the ideal bacterial culture must be able to grow aerobically and be
electrochemically active; utilizing an anode as an alternative electron acceptor while oxi-
dizing metabolites of various carbon sources [2]. In that pursuit, the suitability of Cow
dung, which is bestowed with rich bacterial diversity, as a substrate was examined and
reported by many works from India and abroad [3–16]. However, studies on systematic
screening on metagenomics of the cow dung and bacterial community that constitute
primary film on an electrode in cow dung fed microbial fuel cells are not available. More
specifically, screening of microbial consortia present in the biofilm formed on the elec-
trode surface fitted in MFC has not been done earlier. The present study is an offshoot
of a study on the fabrication and operation of MFC with cow dung slurry to generate
electricity. To identify and isolate potential bacterial species capable of producing higher
electricity, it is essential to find out whether all microbes present in the cow dung are
involved in the process of biofilm formation on the electrode.
For screening the bacterial community present in the cow dung slurry, at the end of
the cycle, 5 ml of the slurry was swabbed from the anode chamber. Similarly, aseptic
swabs were collected from the surface of the anode Electrode and cow dung slurry,
which is analyzed using the Metagenomic tool (16S V3−V4 Metagenome). The slurry
sample reported supporting more varieties of bacterial species. The other sample from
a biofilm, which is formed on the electrode surface, was analyzed by the MALDI-TOF
technique [17]. In the second round, amplicons were tested under 1.2% agarose gel using
the Polymerase chain reaction method. Pie-gram was obtained using matplotlib for the
taxonomy present above the cut-off value of >0.5%. Similarly, the biofilm formed on
the CNT-coated SSM electrode deployed in the anode chamber of the microbial fuel cell
was screened by the MALDI-TOF technique.
3 Samplewise Analysis
To establish the microbiome diversity and species abundances for each of the individual
samples, QIIME analysis has been performed on individual samples as per the protocol
described by [18]. The technique of QIIME (an open-source bioinformatics pipeline)
has been utilized to analyze 16S rRNA gene sequences of Microbial communities, and
it helps to provide a wide range of values from phylum to species. Alpha diversity
and Rarefaction Curves were generated to give the details of the microbial community
present in the sample. The diversity of bacterial species was calculated using Shannon
Index and Simpson’s diversity index method.
18 Y. Thiagarajan et al.
4 Results
The sample was prepared using the common procedure and tested using the mass ion-
ization Technique. A rarefaction plot is carried out for one sample of the V3−V4 region
at a depth of 89,000. The image has been plotted with several sequences per sample in
the x-axis against the diversity index in the y-axis. The samples have been colored by
their names. The given Rarefaction plot portrays the rarefaction bend of species wealth
in V3−V4 tests. This bend is a plot of the absolute number of species with respect to an
element of the number of successions tested. On the left, a precarious incline shows that
an enormous part of the species variety stays to be distinguished. Assuming the bend gets
complimented to one side, it explains that a sensible number of species were examined,
and even more serious inspecting is probably going to yield a couple identifying new
generic groups. Inspecting bends by and large ascent rapidly from the start and afterward
level off due to new species are found per unit of samples gathered.
Fig. 1. Pie chart showing the phyla of bacterial species in cow dung slurry
Fig. 2. Pie chart showing microbial species present in cow dung slurry
Figure 2 shows 64 species under 13 genera. All these species belong to 11 fami-
lies viz. Achleplamatacea, Ruminococcoceae, Erysipelatrideceae, Porpyromonodeceae,
Rhodocyclaeceae, Clostridiaceae, Christenlaeceae, Camplylobacteriaca Plannococco-
ceae, Moraxilaceae, and tissue rellaceae (P2534–1885.BA008) are the unclassified but
showing similarity with strains present in the sample.
From the Rarefaction plot Fig. 3 and Fig. 4, it is observed that rare species are not
reported elsewhere.
Figure 5 shows that about 64 bacterial species were found to be present in cow
dung slurry. Screening of isolates from the electrode surface by MALDI-TOF technique
revealed the presence of only 7 bacterial species. Bacterial species present in biofilm
on electrode were Lysini bacillussphaericus, Bacillus pumilus, B.cereus, B.mojavensis,
Pseudomonas aeruginosa belonging to four genera in the biofilm.
Identification and Analysis of Bacterial Species 21
a) Family
(b) Phylum
c) Species present
Fig. 5. Overall picture of bacterial communities under phylum, class, order, family, genus, and
species and their percentage in cow dung slurry.
22 Y. Thiagarajan et al.
d) Species
e) Species
Fig. 5. continued
In this work, microbial screening using 16SV3–V4 Metagenome revealed that the bac-
terial community present in the cow dung slurry represents four major phyla and 64
species under 13 genera. RFN20-sp. and SMB53-sp., P2534–1885 and BA008-sp are
those unclassified but showing similarity with strains present in the sample. Shannon
Index measures the diversity of species and the Simpson Index measures the species
richness. Both indices are statistically significant. Bacterial species present in cow dung
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Madre, cuando yo me muera
que se enteren los señores.
Pon telegramas azules
que vayan del Sur al Norte.
Siete gritos, siete sangres,
siete adormideras dobles,
quebraron opacas lunas
en los oscuros salones.
Lleno de manos cortadas
y coronitas de flores,
el mar de los juramentos
resonaba, no sé dónde.
Y el cielo daba portazos
al brusco rumor del bosque,
mientras clamaban las luces
en los altos corredores.
14
EL EMPLAZADO
El veinticinco de junio
le dijeron a el Amargo:
Ya puedes cortar si gustas
las adelfas de tu patio.
Pinta una cruz en la puerta
y pon tu nombre debajo,
porque cicutas y ortigas
nacerán en tu costado,
y agujas de cal mojada
te morderán los zapatos.
Será de noche, en lo oscuro,
por los montes imantados
donde los bueyes del agua
beben los juncos soñando.
Pide luces y campanas.
Aprende a cruzar las manos,
y gusta los aires fríos
de metales y peñascos.
Porque dentro de dos meses
yacerás amortajado.
Espadón de nebulosa
mueve en el aire Santiago.
Grave silencio, de espalda,
manaba el cielo combado.
El veinticinco de junio
abrió sus ojos Amargo,
y el veinticinco de agosto
se tendió para cerrarlos.
Hombres bajaban la calle
para ver al emplazado,
que fijaba sobre el muro
su soledad con descanso.
Y la sábana impecable,
de duro acento romano,
daba equilibrio a la muerte
con las rectas de sus paños.
15
ROMANCE DE LA GUARDIA CIVIL
ESPAÑOLA
A Juan Guerrero.
Cónsul general de la poesía.
ROMANCE DE LA GUARDIA CIVIL ESPAÑOLA
•
La ciudad libre de miedo,
multiplicaba sus puertas.
Cuarenta guardias civiles
entran a saco por ellas.
Los relojes se pararon,
y el coñac de las botellas
se disfrazó de noviembre
para no infundir sospechas.
Un vuelo de gritos largos
se levantó en las veletas.
Los sables cortan las brisas
que los cascos atropellan.
Por las calles de penumbra,
huyen las gitanas viejas
con los caballos dormidos
y las orzas de moneda.
Por las calles empinadas
suben las capas siniestras,
dejando detrás fugaces
remolinos de tijeras.
En el Portal de Belén,
los gitanos se congregan.
San José, lleno de heridas,
amortaja a una doncella.
Tercos fusiles agudos
por toda la noche suenan.
La Virgen cura a los niños
con salivilla de estrella.
Pero la Guardia Civil
avanza sembrando hogueras,
donde joven y desnuda
la imaginación se quema.
Rosa la de los Camborios,
gime sentada en su puerta
con sus dos pechos cortados
puestos en una bandeja.
Y otras muchachas corrían
perseguidas por sus trenzas,
en un aire donde estallan
rosas de pólvora negra.
Cuando todos los tejados
eran surcos en la tierra,
el alba meció sus hombros
en largo perfil de piedra.
•
¡Oh ciudad de los gitanos!
La Guardia Civil se aleja
por un túnel de silencio
mientras las llamas te cercan.
I
PANORAMA DE MÉRIDA
II
EL MARTIRIO
III
INFIERNO Y GLORIA