The microscope

Microscope
Learning objectives:
- By the end of the topic you should be able to:

• Use and manipulate a simple light microscope

• Calculate the magnifications and size of biological
specimen using millimetres and micrometres as units

Use and manipulate a simple light microscope

 Microscope
Light Microscope

Use and manipulate a simple light microscope

 Microscope
• Total Magnification from microscope viewing
• Total magnification = eye piece magnification × objective lens magnification
• Example:

Eye piece lens Objective lens Total magnification

×10 ×5 = ×50
×10 × 10 = ×100
×10 × 40 = ×400
×10 × 100 = ×1000

Use and manipulate a simple light microscope

 Calculation of magnification
• From a given specimen

𝑆𝑖𝑧𝑒 𝑜𝑓 𝑡ℎ𝑒 𝑖𝑚𝑎𝑔𝑒 𝑜𝑟 𝑑𝑟𝑎𝑤𝑖𝑛𝑔/𝑚𝑚

• Magnification =
𝑎𝑐𝑡𝑢𝑎𝑙 𝑠𝑖𝑧𝑒 𝑜𝑓 𝑠𝑝𝑒𝑐𝑖𝑚𝑒𝑛 𝑜𝑟 𝑜𝑏𝑗𝑒𝑐𝑡/𝑚𝑚
• Magnification is indicated by ×
Conversions to remember:
- 1 metre (m) = 1000 millimetre (mm)
- 1 millimetre = 1000 micrometre (µm)
- 1 µm = 10-3 (0.001) mm
- 1 cm = 10mm
- 1 mm = 0.1 cm
Calculate the magnifications and size of biological specimen using millimetres and
micrometres as units
Calculate the magnifications and size of biological specimen using millimetres and
micrometres as units
Calculate the magnifications and size of biological specimen using millimetres and
micrometres as units
Calculate the magnifications and size of biological specimen using millimetres and
micrometres as units
 How to prepare a temporary slide

Make temporary slides (wet mounts) of plant and animal cells e.g. epidermal cells
from a leaf or an onion, epithelial cells from human cheek
Make temporary slides (wet mounts) of plant and animal cells e.g. epidermal cells
from a leaf or an onion, epithelial cells from human cheek
 How to prepare a temporary slide

Make temporary slides (wet mounts) of plant and animal cells e.g. epidermal cells
from a leaf or an onion, epithelial cells from human cheek
 How to prepare a temporary slide

Make temporary slides (wet mounts) of plant and animal cells e.g. epidermal cells
from a leaf or an onion, epithelial cells from human cheek