HEp-2 cell patterns

Negative Nuclear Cytoplasmic Mitotic

AC-0 AC-1 to AC-14, AC-29 AC-15 to AC-23 AC-24 to AC-28

Homoge- Dense fine Centromere Speckled Discrete Nucleolar Nuclear Pleomorphic Fibrillar Speckled AMA Golgi Rods and
neous speckled nuclear dots envelope rings
AC-1 AC-2 AC-3 AC-4,5 AC-6, 7 AC-8,9,10 AC-11,12 AC-13,14 AC-15,16,17 AC-19,20 AC-21 AC-22 AC-23
Shown only in
selected slides

Fine Homoge- Dense fine

Topo I speckled Multiple neous Smooth PCNA Linear speckled
Centrosome

AC-29 AC-4 AC-6 AC-8 AC-11 AC-13 AC-15 AC-19 AC-24

Large Few Clumpy Punctate CENP-F Filamentous Fine NuMA Spindle

speckled speckled fibers
AC-5 AC-7 AC-9 AC-12 AC-14 AC-16 AC-20 AC-26 AC-25

Punctate Segmental Discrete Intercellular

dots bridge
Competent-level AC-10 AC-17 AC-18 AC-27
Expert-level report
Mitotic
Metaphase plate is stained chromosomal

AC-28
*Classification tree updated September 2021