Introduction to Microbiology

SCIMA2-B11-Practical

BRIGHT FIELD MICROSCOPY

 Experiment 2B
➢ Record the following for experiment 1.5.1. 1.5.2 and 1.5.3
▪ Density of microbial growth (-, +, ++, +++)

▪ Morphological characteristics of colonies isolated

Morphological characteristics of isolates to look out for in experiment 2A results

 Introduction
➢ Microbes are too small to view with the unaided eyes - microscopic
▪ Microscope to magnify (1000x max - blurry) – Different types?

➢ Light microscope

▪ Important

o Magnification – making an object appear larger than it is

o Resolution – ability to distinguish two close objects clearly

▪ Different parts of the light microscope have specific functions

▪ Dual lens microscope to magnify image - Compound

o Objective (closer to sample) – 4 diff. mag. 4x, 10x, 40x &100x

o Ocular (closer to the eye) – 8x -15x

▪ Scanning power (4x), Low power (10x), High power (40x) and Oil immersion
(100x)
 Introduction
➢ Different parts of a compound light microscope

▪ Eyepiece (monocular/binocular) – magnify the image of the specimen

▪ Objectives – magnify and resolves image of the specimen

▪ Stage – secures the specimen slide in place

▪ Light source – illuminates the specimen

▪ Condenser – focus the light on the specimen

▪ Focus knobs (either controls the stage or the objective lens)

o Coarse – bring the specimen to view

o Fine – sharpen the image of the specimen

 Experiment 2B

Viewing microbial samples under the microscope

Experiment 2B

Different microbial morphology under the microscope

 Experiment 2B
➢ Bright Field Microscopy
▪ Article, SOP, Experimental design?

➢ LO

▪ Ability to work aseptically in a microbiology lab

➢ AC

▪ Perform basic microbiological techniques

➢ Recommended book chapters

▪ Chapter 1 – The microbial world and you

▪ Chapter 3 – Observing microorganisms through a microscope

 Experiment 2B rules
➢ Come into the lab with

✓ Clean acid resistant lab coat

✓ Closed shoes that covers feet

✓ Long hair tied to the back

✓ Neatly trimmed nails

✓ A fine point permanent marker

➢ Work in subgroup of 4 students (Except stated otherwise)

✓ Pre-lab exercise and worksheets written individually

➢ Materials and methodology from page 13

▪ SOP – Appendix E & F

Risks, hazards and
control measures?
Experiment 2B
 NEXT – Practical 3A
➢ Colony isolation techniques

▪ Serial dilution, Pour plate, Spread plate and Streaking methods

▪ SOP – Appendix A, B, & C and Experimental design

 NEXT – Practical 3A

Source: https://slideplayer.com/slide/7863676/, https://microbeonline.com/pour-plate-method-principle-procedure-

uses-dis-advantages/