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Eur J Oral Sci 2018; 1–7 © 2018 Eur J Oral Sci

DOI: 10.1111/eos.12585 European Journal of


Printed in Singapore. All rights reserved
Oral Sciences

Eun S. Park1,2, Hyeon W. Yim1,3,


Progressive muscle relaxation therapy Kang S. Lee1,3
1
Department of Public Health, Graduate

to relieve dental anxiety: a randomized School of the Catholic University of Korea,


Seoul; 2Department of Dental Hygiene, The
Hyejeon College of Korea, HongSeong-Eup;
controlled trial 3
Department of Preventive Medicine, College
of Medicine, The Catholic University of Korea,
Seoul, Korea

Park ES, Yim HW, Lee KS. Progressive muscle relaxation therapy to relieve dental
anxiety: a randomized controlled trial.
Eur J Oral Sci 2018; 00: 1–7. © 2018 Eur J Oral Sci
Dental anxiety causes patients to refuse or delay treatment, which may exacerbate
oral diseases. The aim of the current randomized controlled trial was to determine
whether progressive muscle relaxation therapy could relieve dental anxiety. The trial
included 68 periodontal patients with dental anxiety scores of ≥13 who were ran-
domly assigned to either an intervention group or a control group (n = 34 per
group). The intervention group was administered progressive muscle relaxation ther-
apy for 20 min and oral health education for 15 min before periodontal treatment Kang Sook Lee, Department of Preventive
Medicine, College of Medicine, The Catholic
once per week for 4 wk. The control group was provided with oral health education
University of Korea, 222 Banpo-daero,
only, for the same duration. Changes in dental anxiety, depression symptoms, blood Seocho-gu, Seoul 137-701, Korea
pressure, heart rate, and salivary cortisol were evaluated 4 wk and 3 months after
the intervention. The intervention group exhibited statistically significantly greater Email: leekangs@catholic.ac.kr
reductions in dental anxiety scores than did the control group at the 4-wk ( 3.82 vs.
0.89) and 3-month ( 4.22 vs. 0.28) assessments. They also exhibited significantly Key words: dental health education;
greater reductions in depression symptoms, systolic and diastolic blood pressure, depression; oral health education;
pulse rate, and salivary cortisol levels at both time-points. Progressive muscle relax- periodontal patients
ation therapy relieves tension and anxiety in dental patients. Accepted for publication October 2018

Dental anxiety is a complex phenomenon with physical, associated with symptoms of depression. In one previ-
mental, and social aspects (1). High levels of dental ous study (7), five patients exhibited clinically signifi-
anxiety can cause patients to experience stress and cant symptoms of depression during dental visits, and
refuse or delay treatment. This damages oral health antidepressant medication caused reduced saliva pro-
and leads to worsening of oral diseases. Dental anxiety duction, poor oral hygiene, and periodontal disease-
also interferes with successful treatment by causing related bacterial infection. Such physiological changes
increased perceptions of pain after treatment, thereby can lead to periodontal disease as a result of abnor-
delaying optimal recovery (2). The prevalence of dental mal immune responses (8). Thus, clinicians must be
anxiety in adult patients is approximately 20% (3), with aware of psychological parameters in patients with
5%–7% of patients experiencing extremely high levels dental anxiety.
of dental anxiety (4). Muscle relaxation therapy reduces the activity of the
SANTUCHI et al. (5) reported relatively high levels of sympathetic nervous system when stimulated by psy-
dental anxiety in periodontal patients, which were asso- chological and physiological responses, resulting in low
ciated with poor gingival status. Dental anxiety can be heart rate, low respiratory rate, and low blood pres-
reduced with the use of injected or inhaled sedatives. sure. In addition, it can effectively regulate the periph-
However, such drugs entail a risk of side effects, includ- eral and central nervous systems, thereby lowering
ing respiratory depression, gastrointestinal disorders, stress, anxiety, and depression levels, and thus is effec-
nausea, vomiting, and allergic reactions. Additionally, tive in treating several health problems (9). In previous
sedatives increase the cost of dental treatment, and the studies, Dental Anxiety Scale (DAS) scores were
body takes a long time to absorb injected sedatives, so reported to be significantly lower in a group receiving
they are of limited use in clinical settings. Therefore, progressive muscle relaxation therapy than in the corre-
drug-free methods are increasingly being used to ame- sponding control group (10), and patients receiving
liorate tension and moderate dental anxiety during muscle relaxation therapy reported that the dental anxi-
treatment (6). ety relief persisted during the subsequent dental visit
High dental anxiety is associated with oral health (11). ARMFIELD et al. (12) also suggested that progres-
issues and avoidance of dental care, and it can lead sive muscle relaxation therapy enables successful man-
to mental anxiety. In turn, poor oral health is agement of dental anxiety.
2 Park et al.

The purpose of this study was to establish the basis up to 10%, 35 patients were enrolled in each group to
of dental anxiety mitigation by progressive muscle ensure an adequate final sample size.
relaxation therapy in patients with dental anxiety.
The present randomized controlled trial (Clinical
Participants
Research Information Service trial registration number
KCT0001648) investigated whether progressive muscle The present study was approved by the Institutional
relaxation therapy could relieve dental anxiety, and if Review Board of The Catholic University of Korea. The
so, whether the anxiety-relieving effects of the therapy participants were 70 patients who visited a dental clinic in
lasted for 3 months in periodontal patients. The pri- Incheon, Republic of Korea, between 4 September 2015
mary outcome was relief from dental anxiety 3 months and 30 January 2016 for periodontal treatment. The inclu-
after commencement of the intervention, and the sec- sion criteria were as follows: chronic periodontal disease;
age 30–59 yr; consent to participate after being briefed on
ondary outcomes were reductions in dental anxiety, the purpose of the study; a DAS of ≥13 on the Corah den-
symptoms of depression, blood pressure, heart rate, tal anxiety scale (16); dental treatment planned for 5 wk
and salivary cortisol concentration, which are consid- or longer; one or more periodontal pockets ≥4 mm in
ered indicators of stress (13, 14), at 4 wk and 3 months depth; the absence of systemic diseases that could affect
after intervention commencement. periodontal disease; sightedness; literacy; and the ability to
understand the study and answer questions. Two patients
who did not meet the inclusion criteria were excluded: one
had a DAS of <13; and the other was unavailable for the
Material and methods collection of saliva samples between noon and 4 PM. Thus,
Sample size calculation 68 patients were ultimately included in this trial.
The patients were randomly assigned to either an inter-
Dental anxiety was set as the primary evaluation outcome vention group or a control group (n = 34 per group). Two
for calculating sample size. Based on existing research patients in the intervention group and three in the control
(15), a two-sided a-value of 0.05, a power of 0.80, and an group dropped out after intervention commencement
effect size of 0.72, a sample size of 31 was originally calcu- because they could not visit the clinic at the required time
lated as appropriate for intergroup comparison of mean for personal reasons. Thus, a total of 63 patients com-
anxiety scores. Based on an anticipated drop-out rate of pleted the trial (Fig. 1).

Fig. 1. Flow diagram of subject selection and allocation in accordance with Consolidated Standards of Reporting Trials
(CONSORT) guidelines. DAS, dental anxiety scale.
PMR therapy to treat dental anxiety 3

Study flow toothbrushing. During week 2, a clinical specialist with


over 10 yr of experience explained proper toothbrushing
All participants were briefed on the purposes and methods methods (i.e. the Bass, Rolling, and Watanabe methods)
of the study before being assigned to one of the two (20). The patients then practiced these methods on a tooth
groups by a third party using random number generation model, and their mistakes were identified and corrected
software. A piece of paper indicating the assigned group during that process. During week 3, the patients were
was inserted into a sealed envelope, and participants were informed about interdental care and instructed on the use
asked to open one of the sealed envelopes in a predeter- of oral hygiene products relevant to their individual peri-
mined order at their first visit. An evaluator performed all odontal conditions. During week 4, they were again
measurements without knowing the group allocation of instructed on methods of toothbrushing and the use of
any of the participants. oral hygiene products.
Dental anxiety levels were evaluated using a dental anxi-
ety questionnaire (16), and psychological changes were
assessed using a depression questionnaire (17). Participants Evaluations
were administered these questionnaires at baseline and at
4 wk and 3 months after intervention commencement. To The primary evaluation was the change in dental anxiety
evaluate salivary cortisol concentrations, unstimulated sal- level at 3 months after starting the intervention, and the
iva samples were collected. Blood pressure and heart rate secondary evaluations were the changes in depression, vital
were also recorded. All evaluations were performed signs, and salivary cortisol levels at 3 months after begin-
between 12 PM and 4 PM (18), and visits were scheduled ning the intervention. The primary outcome was measured
according to patient availability. The intervention group via the DAS developed by CORAH (16), which is the most
received progressive muscle relaxation therapy and oral common measure for evaluating dental patient anxiety. It
health education once per wk for 4 wk, and the control comprises four questions that describe risky and threaten-
group received oral health education only, also once per ing situations during dental treatment. Each question is
week for 4 wk. scored from 1 (not anxious at all) to 5 (terrified); thus, the
When administering progressive muscle relaxation ther- total score ranges from 4 to 20. Patients with DAS scores
apy, we adopted a program used to alleviate dental anxi- of ≥13 are deemed to experience dental anxiety. Cron-
ety in patients at a psychiatric department of a university bach’s a coefficient for this index in the current study was
hospital in South Korea. This intervention program has 0.83.
been shown to relieve fear and stress (19). Furthermore, it Symptoms of depression were evaluated using the Beck
is easy to learn and practice, requires no special equip- Depression Inventory (17), which evaluates clinical symp-
ment, and can be delivered anywhere at any time. Partici- toms of depression and comprises 21 questions, with
pants are advised to wear comfortable clothing and asked responses to each question measured on a 4-point Likert
to remove glasses and footwear. They are seated in a scale (0–3). The total score ranges from 0 to 63, with
relaxed position on a comfortable chair in a quiet, dimly higher scores indicating higher levels of depression. The
lit room and asked to listen to a voice delivered through a associated Cronbach’s a coefficient in the current study
headset playing a recording of a progressive muscle relax- was 0.91.
ation therapy script. During the recording, the patients are Patients were prohibited from consuming food and caf-
asked to close their eyes, breathe deeply, and imagine feine, smoking, and brushing their teeth for 1 h before sal-
peaceful scenery. They are then asked to progressively iva collection. Saliva samples were collected between 12 PM
tense and relax different muscle groups throughout their and 4 PM (18). Patients were instructed to rest in a com-
body—from global to local muscles, including those in the fortable position, without speaking, for 5 min before saliva
hands, arms, face, neck, shoulders, abdomen, legs, and was collected. To avoid mixing saliva with food residue or
feet. This develops muscle awareness and gives patients blood, patients were asked to gargle with cold water
the ability to differentiate between tensed and relaxed mus- before collection. Once the mouth was thus naturally
cles (19). Participants in the intervention and control moistened, patients were asked to spit into polypropylene
groups received the same periodontal treatment and oral specimen containers. Saliva samples containing blood were
health education. In both groups, the education was excluded.
administered by a single dental hygienist in a separate den- The saliva samples (2 ml) were stored at 20°C and
tal office. transported to the testing laboratory while frozen, before
When conducting the periodontal disease treatment, analysis. The samples were evaluated using a high-sensitiv-
clinicians ensured that patients were able to manage their ity salivary cortisol ELISA (Salimetrics LLC, State Col-
periodontal health effectively. In addition, patients were lege, PA, USA) (21).
instructed on proper toothbrushing practices in order to Blood pressure was measured using an automated blood
ensure appropriate management of their periodontal pressure monitor (MX3; Omron, Tokyo, Japan). Patients
health. Patients in the test group received progressive mus- were prohibited from consuming caffeinated food, under-
cle relaxation therapy for 20 min and oral health educa- going intensive exercise, and smoking for 1 h before mea-
tion for 15 min. Intervention was administered once per surements were made. During measurements, patients were
week for a total of 4 wk (i.e. at baseline, and 1, 2, and instructed to rest for at least 10 min, and then blood pres-
3 wk after intervention commencement). Periodontal treat- sure was measured 3 min later and again 5 min after the
ment was performed with scaling once, and root planing first measurement. The average value of the two measure-
was performed three times. A total of four sessions were ments was used for analysis. If the margin of error was
performed once per week. Each week, periodontal treat- ≥10 mmHg, the measurement was repeated and the aver-
ment was performed after intervention. age of the two values with the smallest margin of error
During the first week, patients were informed about was used for analysis (22).
proper toothbrush selection, storage and replacement of Periodontal status was measured at the initial examina-
toothbrushes, toothbrushing time, and the importance of tion. A gingival index is the most commonly utilized
4 Park et al.

method for gauging gingival inflammation while evaluating Table 1


periodontal status. In the present study, the gingival
Baseline characteristics of the study participants
indices of the buccal, lingual, mesial, and distal surfaces of
six teeth were scored on a 4-point scale ranging from 0 to Study group
3 points (23).
Using a Williams probe (Hu-Friedy, Chicago, IL, USA), Intervention Control
Variable (n = 34) (n = 34)
periodontal pocket depth (PPD) was measured in millimeters
from the gingival ridge to the bottom of the pocket at the fol- Age (yr) 42.4  10.0 43.2  9.9
lowing six sites around each tooth, except the third molar: Sex
the mesiobuccal, midbuccal, distobuccal, distolingual, midlin- Male 9 (26) 8 (24)
gual, and mesiolingual sites. The largest PPD value measured Female 25 (74) 26 (76)
for each site, for each tooth, was recorded, and the mean Education level
value of all teeth was used for the analysis (24). ≤Middle school 3 (9) 5 (15)
Gingival bleeding on probing (BOP) with a periodontal High school 13 (38) 16 (47)
probe is considered a sign of gingivitis. In the present ≥College 18 (53) 13 (38)
study, the six sites around each tooth for which PPD was Income (1000 won)
measured were checked for bleeding 10–30 s after probing. <1000 3 (9) 2 (6)
Bleeding on probing was recorded for each tooth as either 1000–2999 22 (65) 19 (56)
absent (0) or present (1), if even only one site showed ≥3000 9 (26) 13 (38)
bleeding. The number of teeth with gingival bleeding was Clinical parameters
calculated as a percentage of the total dentition to yield a Gingival index 1.3  0.6 1.4  0.5
BOP percentage (25). Probing pocket depth (mm) 2.7  0.5 2.7  0.4
Percantage of teeth with 32.0  6.9 32.5  6.4
bleeding on probing
Data analysis Psychological parameters
Dental anxiety scale 13.7  1.1 13.5  0.9
Data analysis was performed using SAS version 9.4 (SAS Beck depression inventory 31.2  8.5 29.7  6.3
Institute, Cary, NC, USA). Summary statistics are pre- Systolic blood pressure 126.6  16.3 120.5  9.6
sented as number (%) for categorical variables and as (mmHg)
mean  SD for continuous variables. Baseline character- Diastolic blood pressure 79.9  10.4 77.4  6.3
istics, as well as the patients’ clinical and psychological (mmHg)
parameters, were compared using the Wilcoxon rank-sum Pulse rate (times min 1) 78.1  13.5 72.6  10.0
test, the chi-square test, or Fisher’s exact test. Baseline Salivary cortisol level (ng ml 1) 2.6  2.1 2.3  1.4
data were compared with data obtained at 4 wk and Data are presented as n (%) or mean  SD.
3 months after intervention commencement using the The P-values of significant differences between the groups were
paired t-test or Wilcoxon signed-rank test. To confirm the determined using the chi-square test, Fisher’s exact test, the Stu-
efficacy of the therapy, time-dependent changes in the two dent’s t-test, or the Wilcoxon rank-sum test.
groups were compared using analysis of covariance. All
participants registered in this randomized clinical trial
were analyzed using the intention-to-treat (ITT) method. 3 months after intervention commencement. Four
In cases of missing data, the last observation carried for- weeks after intervention commencement, the decrease
ward (LOCF) method was utilized. All values of P <0.05 in dental anxiety was significantly greater in the inter-
were considered statistically significant. vention group than in the control group ( 3.8 vs.
0.9; P < 0.001). Three months after commencement
of the intervention, the decrease in dental anxiety was
Results still significantly greater in the intervention group than
in the control group ( 4.2 vs. 0.3; P < 0.001). These
As shown in Table 1, there was no significant difference results indicate that in both groups, dental anxiety had
in the mean age between the intervention group lessened 4 wk after intervention commencement and
(42.4 yr) and the control group (43.2 yr). The overall that the effects of muscle relaxation therapy lasted for
proportion of women in the study was 73%, and there at least 3 months.
were no significant differences in education level or As shown in Fig. 2, the decrease in depression symp-
income between the two groups. toms was significantly greater in the intervention group
At baseline, there were no significant differences in than in the control group ( 1.3 vs. +0.5; P < 0.001) at
the level of gingivitis, PPD, or BOP between the two the 4 wk assessment, and 3 months after commence-
groups, and there were no significant differences in the ment of the intervention, the decrease was still signifi-
psychological parameters (dental anxiety or symptoms cantly greater in the intervention group than in the
of depression) or physiological parameters (diastolic control group ( 2.2 vs. +1.0; P < 0.001).
blood pressure, heart rate, or salivary cortisol levels) The decrease in systolic blood pressure 4 wk after
between the two groups, with the exception of systolic intervention commencement was significantly greater in
blood pressure, which was significantly higher in the the intervention group than in the control group
intervention group. ( 11.0 vs. +3.2; P < 0.001), and 3 months after com-
As shown in Table 2, in the intervention group, the mencement of the intervention, the decrease was still
mean DAS score decreased from 13.7  0.2 at baseline significantly greater in the intervention group than in
to 9.9  0.2 and 9.6  0.2, respectively, at 4 wk and the control group ( 11.9 vs. +4.5; P < 0.001).
PMR therapy to treat dental anxiety 5

Table 2
Comparison of dental anxiety levels at 4 weeks and 3 months post-intervention

Study group
Variable Intervention (n = 34) Control (n = 34) P-value

DAS score at baseline 13.7  0.2 13.5  0.2


DAS score at 4 wk 9.9  0.2 12.6  0.2
DAS score at 3 months 9.6  0.2 13.2  0.3
Change in DAS at 3 months* 4.2 ( 4.6 to 3.8) 0.3 ( 0.7 to 0.1) <0.001
Change in DAS at 4 wk† 3.8 ( 4.1 to 3.5) 0.9 ( 1.2 to 0.6) <0.001

Values are given as mean  SD or mean difference (95% CI).


The P-values of significant differences between the groups were determined using the chi-square test, Fisher’s exact test, the Student’s t-
test, or the Wilcoxon rank-sum test.DAS, dental anxiety scale.Statistical analysis was performed by analysis of covariance, adjusted in
accordance with the baseline value of each parameter. The P-values of significant differences in parameters between baseline and 4 wk or
3 months post-intervention were determined using the paired t-test or Wilcoxon signed-rank test.
*Primary outcome.

Secondary outcome.
DAS, dental anxiety scale.

Fig. 2. Comparisons of depression levels (A), systolic (B) and diastolic (C) blood pressure, pulse rate (D), and salivary cortisol
levels (E) in the intervention and control groups at 4 wk and 3 months after the intervention. f/u, follow up.

The decrease in diastolic blood pressure 4 wk after significantly greater in the intervention group than in
intervention commencement was significantly greater in the control group ( 7.4 vs. +0.5; P < 0.001).
the intervention group than in the control group ( 6.5 The decrease in pulse rate 4 wk after intervention
vs. +1.7; P < 0.001), and 3 months after commence- commencement was significantly greater in the interven-
ment of the intervention, the decrease was still tion group than in the control group ( 6.7 vs. +2.2;
6 Park et al.

P < 0.001), and 3 months after commencement of the studies have shown (27) that pharmacological interven-
intervention, the decrease was still significantly greater tion results in risk factors and adverse effects; notably,
in the intervention group than in the control group persistent dental anxiety relief has been found only
( 7.3 vs. +0.1; P = 0.002). from psychological interventions. Therefore, it is neces-
The decrease in salivary cortisol levels 4 wk after sary to use psychological intervention to alleviate den-
intervention commencement was significantly greater tal anxiety.
in the intervention group than in the control group The intervention group also exhibited reductions in
( 1.0 vs. 0.1; P = 0.007), and 3 months after com- symptoms of depression 4 wk and 3 months after start-
mencement of the intervention, the decrease was still ing the intervention, which is consistent with the results
significantly greater in the intervention group than in of a previous study in which patients with atopic der-
the control group ( 1.0 vs. +0.3; P = 0.002). matitis exhibited a significant decrease in depression
These results indicate that in both groups, dental symptoms 1 month after receiving muscle relaxation
anxiety, depression symptoms, systolic blood pressure, therapy twice daily for 4 wk (28). These results suggest
diastolic blood pressure, pulse rate, and salivary corti- that progressive muscle relaxation therapy effectively
sol levels had become reduced 4 wk after intervention alleviates depression stemming from dental anxiety.
commencement and that the effects of muscle relax- The authors of another study argued that depression
ation therapy lasted for at least 3 months. symptoms are strongly associated with dental anxiety
and that they have a stronger effect on particular types
of anxiety (7).
In patients who are extremely afraid of dental treat-
Discussion
ment, symptoms of depression are caused by a combi-
The present study examined the effects of progressive nation of subjective stress from past experiences and a
muscle relaxation therapy on periodontal patients’ den- negative emotional state. Participants treated using pro-
tal anxiety relief maintenance at 4 wk and 3 months gressive muscle relaxation therapy appear to experience
after intervention, and clearly demonstrated an associa- a positive emotional state repeatedly, which reduces
tion between progressive muscle relaxation therapy and dental anxiety, stabilizes patient behavior, and conse-
the relief of dental anxiety. This result is consistent quently ameliorates depression symptoms. Therefore, to
with that of a previous study which reported a signifi- reduce patients’ dental anxiety, dentists should be
cant decrease in anxiety in fracture patients who mindful of various psychological factors, particularly
received progressive muscle relaxation therapy every symptoms of depression.
morning and afternoon during hospitalization (13). The The intervention group in the present study exhibited
findings are also concordant with those of another a clinically significant decrease in salivary cortisol
study in which there was a significant reduction in anxi- levels. This is concordant with a previous study in
ety in patients with cancer who received therapy for which patients with breast cancer exhibited lower corti-
10 wk (26). sol levels after being administered therapy once per
Patients in the control group who did not receive week for 10 wk, whereas a control group that did not
progressive muscle relaxation therapy also experienced receive relaxation therapy exhibited an increase in corti-
a temporary decrease in dental anxiety at 4 wk post- sol levels (29). In the present study, because the two
intervention. However, at 3 months post-intervention, groups had similar gingival states and demographic
dental anxiety levels in the control group had increased characteristics, neither of these factors could have
again; by contrast, patients in the intervention group affected the variables evaluated. Therefore, progressive
continued to experience dental anxiety relief, which muscle relaxation therapy can effectively reduce stress
indicated the effectiveness of progressive muscle relax- and it appears to alleviate dental anxiety and help
ation therapy. The effects of therapy were observed to patients feel relaxed and calm, leading to decreased cor-
have lasted until 3 months post-intervention. On the tisol levels.
basis of these findings, it may be concluded that pro- There was a low drop-out rate in the present study
gressive muscle relaxation therapy administered on a (2.9%), and the LOCF method was used in cases of
long-term basis under constant monitoring to ensure missing data, for ITT analysis. Nevertheless, outcomes
moderation produces long-lasting positive effects. We did not differ between the ITT analyses with and with-
have found that the progressive muscle relaxation out the missing variables, perhaps because the study
response can facilitate stress relief in patients with den- used randomized allocation to prevent undue influence
tal anxiety. The DAS is an objective, reliable, and vali- by a third confounding factor and to minimize preju-
dated measure that is widely used in adult dental dice or bias, ensuring an objective and scientific
anxiety research worldwide. It evaluates dental anxiety approach toward evaluating the effect of progressive
using specific questions about the respondent’s dental muscle relaxation therapy on dental anxiety.
situation. The original study conducted by CORAH (16) The present study had several limitations. Because
demonstrated that the internal consistency of the dental the participants were enrolled from a single dental
anxiety questions was high (standardized Cronbach’s clinic, the generalizability of the intervention group,
a = 0.86), and therefore the scale can be considered as and consequently the external validity of the findings,
accurate. Psychological and pharmacological interven- may be limited. Psychological parameters, including
tions are effective in reducing dental anxiety. However, dental anxiety, were evaluated using self-reported
PMR therapy to treat dental anxiety 7

questionnaires and may therefore have been subjective. 11. LAMB DH, STRAND KH. The effect of a brief relaxation treat-
However, physiological indicators of anxiety, such as ment for dental anxiety on measures of state and trait anxi-
ety. J Clin Psychol 1980; 36: 270–274.
blood pressure, heart rate, and salivary cortisol levels, 12. ARMFIELD JM, HEATON LJ. Management of fear and anxiety
were objective. Studies involving longer follow-up peri- in the dental clinic: a review. Aust Dent J 2013; 58: 390–407.
ods are required to determine the duration of the 13. TAKAI N, YAMAGUCHI M, ARAGAKI T, ETO K, UCHIHASHI K,
effects of progressive muscle relaxation therapy. NISHIKAWA Y. Effect of psychological stress on the salivary
In the present study, the administration of progres- cortisol and amylase levels in healthy young adults. Arch Oral
Biol 2004; 49: 963–968.
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dental hygienist effectively alleviated dental anxiety in SB. Assessment of the changes in the stress-related salivary
periodontal patients for at least 3 months after the cortisol levels to the various dental procedures in children. J
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15. LI Y, WANG R, TANG J, CHEN C, TAN L, WU Z, YU F, WANG
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tal patients. depression of pulmonary arterial hypertension patients. Evid
Based Complement Alternat Med 2015; 2015: 792895.
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and wish to acknowledge the study assistants for their help with Res 1969; 48: 596.
patient interviews. 17. BECK AT, WARD CH, MENDELSON M, MOCK J, ERBAUGH J.
An inventory for measuring depression. Arch Gen Psychiatry
Conflict of interests – The authors have no conflict of interests to 1961; 4: 561–571.
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