Acidic and Basic Solution Neutralization SOP

Purpose:
To provide a safe and effective procedure for the neutralization of acidic and basic solutions
generated in the laboratory.

Scope:
This SOP covers the neutralization of excess or unwanted acidic and basic solutions to ensure
their safe disposal and minimize the risk of environmental and personnel hazards.

Procedure:

Identification of Acidic and Basic Solutions:

a. Identify all acidic and basic solutions that require neutralization.
b. Determine the concentration and volume of the solutions.

Neutralization:
a. Wear appropriate personal protective equipment (PPE), such as lab coat, gloves, and safety
goggles.
b. For acidic solutions:
i. Slowly add a neutralizing agent, such as sodium hydroxide (NaOH) or calcium carbonate
(CaCO3), while stirring the solution.
ii. Continue adding the neutralizing agent until the solution reaches a pH between 6.0 and 8.0.
c. For basic solutions:
i. Slowly add a neutralizing agent, such as hydrochloric acid (HCl) or acetic acid
(CH3COOH), while stirring the solution.
ii. Continue adding the neutralizing agent until the solution reaches a pH between 6.0 and 8.0.

Disposal:
a. Once the solution is neutralized, it can be safely disposed of down the drain with copious
amounts of water.
b. Consult the institution's guidelines or local regulations for the disposal of neutralized
solutions.

Documentation:
a. Keep records of the neutralization process, including the initial and final pH values, the
volume and concentration of the solutions, and the amount of neutralizing agent used.
b. Document any deviations from the standard procedure or issues encountered during the
neutralization process.

Training and Safety:

a. Provide appropriate training to all laboratory personnel on the safe handling and
neutralization of acidic and basic solutions.
b. Ensure that the necessary equipment and neutralizing agents are readily available in the
laboratory.