TreeAzin Systemic Insecticide: a New Option for Pest Management

J. Meating BioForest Technologies Inc. December 1, 2010

Acknowledgements

Canadian Forest Service;

B. Helson, J. McFarlane, D. Thompson

Ontario Ministry Natural Resources National Research Council IRAP;

TreeAzin Systemic Insecticide

5% technical systemic formulation
Rapid uptake and translocation Effective in deciduous and conifer species

IP developed and owned by the CFS Under worldwide license to BioForest Technologies

TreeAzin Systemic Insecticide

Neem-based reduced risk bioinsecticide; AI=Azadirachtin not Neem Oil High toxicity to wide range of damaging insect pests; Very low mammalian and bird toxicity;

Azadirachtin: Modes of Action

Immature stages:
IGR inhibits development

Mature stages:
Repellant Reduced fecundity

TreeAzin: Regulatory Status in the U.S.

U.S.
USEPA registration October 2008 (Reg # 82996-1)
Professional Use Master Label Homeowner use

State registrations ~25

Canada
Emergency Registration for EAB (ON, QU) Full Registration 2011?

Environmental Fate
What happens when treated leaves fall to the ground or into water bodies?
AZA concentrations almost undetectable No impact on soil organisms No impact on aquatic organisms

TreeAzin: OMRI Listed (Dec. 2008)

TreeAzin Efficacy Trials 2002-2010

Host Species
Red pine Jack Pine White pine White spruce Balsam fir Cedar Lodgepole pine Red and Bur oak Trembling aspen Ash Maple White birch Hemlock
Pest Species Emerald ash borer Gypsy moth Pine false webworm Introduced pine sawfly Cedar leafminer Forest tent caterpillar Eastern spruce budworm Jack pine budworm Birch leafminer Bronze birch borer Hemlock wooley adelgid *Mountain pine beetle *Asian longhorned

beetle

TreeAzin Label Pests

Emerald ash borer Gypsy moth Forest tent caterpillar Spruce budworm Jack pine budworm Leafminers Sawflies Hemlock Woolly Adelgid Future additions: Bronze Birch Borer Asian Longhorned Beetle Japanese Beetle Pine Shoot Beetle Winter moth

Emerald Ash Borer

EAB Larval Development and Adult Emergence

Number of Developed Adult EAB Beetles in Imidacloprid and Azadirachtin Treated Potted Ash Trees
Number of Beetles
14 12 10 8 6 4 2 0 0 0.0017 0.0035 0.007 0.015 0.03 0.06

Imidacloprid Azadirachtin

Concentration in g of AI/cm dbh

EAB Larval Development and Adult Emergence

Number of Incomplete EAB Beetle Galleries in Imidacloprid and Azadirachtin Treated Potted Ash Trees
Number of Galleries

12 10 8 6 4 2 0 0 0.0017 0.0035 0.007 0.015 0.03 0.06

Imidacloprid Azadirachtin

Concentration in g of AI/cm dbh

EAB Fecundity & Fertility

Control 40 35 30 25 20 15 10 5 0 Eggs laid Eggs hatched 12.3 5.4 0.09 36.5 TreeAzin

99% reduction in EAB population for Year X+1

EAB: Evidence for Two-Years Protection with TreeAzin

Oakville Study, March 2010

Results: Year 2
Treated August 2008 Feb. 2010: Trees assessed for woodpecker feeding sites and 2009 EAB galleries (n=209)

Woodpecker Feeding Site Density

Treated 25 23 Untreated

20

19

No. trees

15

10 5 5 2 0 0 1-5 Woodpecker feeding sites >5 1

EAB Galleries: Yr 2

EAB/100cm sq

Avg Lt. (cm)

0.47

0.5 0.45 0.4

12
10.2

10

Galleries/100cmsq

0.35 0.3 0.25 0.2 0.15 0.1 0.05 0 0.08

Avg gallery Length (cm)

4
2.4

0
TreeAzin Control

TreeAzin

Control

Efficacy: Oakville 2008-2010

Heavily infested tree

Conclusions: TreeAzin
No direct mortality of feeding adults; Some gallery formation; Highly active at inhibiting larval development and preventing adult emergence at very low dosages; Reduced fertility and fecundity of adults feeding on leaves.

CFS Conclusions: TreeAzin

Early summer injections at 12.5ml/in. (5ml/cm) dbh at early stages of an infestation should provide very effective control of EAB. McKenzie et al. (2010) Azadirachtin: an effective systemic insecticide for control of Agrilus planipennis (Coleoptera: Buprestidae). J. Econ. Entomol. 103 (3) 708 -717.

TreeAzin: Municipal Programs

EABDSS implementation

Detection survey training and analysis

BioForest Technologies Inc.

Thank you.
www.bioforest.ca