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Histopathology 1, Direct 2022
Histopathology 1, Direct 2022
Section A
1. Explain five specific safety precautions considered once in a histopathology Laboratory.
2. Explain any ten qualities of a good fixative.
3. Describe the components of a fully filled request form.
4. Describe the purpose of tissue processing, the stages involved and the factors affecting
the rate of tissue processing.
5. What is the use of the water bath and diamond pencil in histology?
6. Describe the principle of H&E staining
7. Why do most laboratories routinely use a resinous mounting medium?
8. Describe why sections need to be coloured with dyes and how these dyes bind to tissues.
9. Toluidine blue will stain mast cell granules red. What is the name of this phenomenon?
Why does the colour change occur?
10. A small amount of mordant causes staining but an excess of mordant removes the staining.
Explain this oddity.
Section B
1. Dr Sengoba from Hope Medical Centre sent a histology sample to your histopathology
Laboratory for analysis.
a) Describe in detail the steps you would follow to process this sample to the point of
presenting slides to the pathologist for reading/resulting.
b) If one of the tissue fragments submitted was bony. How would you treat this sample?
2. Jackline a Histo-technologist received a histology sample from Clarke International
University hospital. On receiving the sample, it was found to be unfit for processing and
therefore rejected the sample.
a) Explain what Jackline could have based on to reject the sample?
b) A day later, Jackline received the same sample and was identified as an excision biopsy
of a back tumour. Explain the key areas she would look at when grossing the sample
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