Lab 3.

Cells of the immune system

Objective:

- Identify the leucocytes (WBC)

- Determine the relative numbers of the types of leucocytes
Lab 3. Cells of the immune system

(Leucocytes)
Lab 3. Cells of the immune system
 Lab 3. Cells of the immune system

1. Preparation of blood smear:

Preparing a blood smear for inspecting white cells
 Lab 3. Cells of the immune system
2. Giemsa stain of blood smear:
Components Stained location Stained color
Methylene blue Nucleus Blue to purple
Azure B Nucleus Blue to purple
Eosin Y Cytoplasm Orange to pink

Stained granulars’
color
Neutrophil Fairly pink
Eosinophil Red
Basophil Blue
 Lab 3. Cells of the immune system

2. Giemsa stain of blood smear:

Submerge blood smear slide in 100% Methanol | 5 mins

↓
Drain well by touching end of slide on kitchen towel and air dry
↓
Stain the slide with diluted Giemsa stain (1:10, vol/vol) | 20 mins
↓
Gently wash with water by dipping the slide repeatedly | 30 secs
↓
Drain well by touching end of slide on kitchen towel and air dry
 Lab 3. Cells of the immune system

3. Differential blood leukocyte count:

Place the blood smear under the microscope objective (4x – 40x)
↓
Observe different morphological type of white cell
↓
Examine 50 to 100 different white cells → Estimate the percentage of each
cell type present in the smear
↓
Record your results
Observing under microscope:

Total:4
1 lymphocyte
3 eosinophils

Total:6
1 lymphocyte
2 neutrophils
1 basophil
1 eosinophil
1 monocyte
2 counting times: total 10/100
2 lymphocytes, 2 neutrophils, 4 eosinophils, 1 basophil, 1 monocytes
Lab 3. Cells of the immune system
 Lab 3. Cells of the immune system

Report:

➢ Keynotes of today’s class

➢ Labwork report
➢ Feedback for Instructors

➢ Overview next week’s practice