1/28/24, 10:22 AM Production of Single-Cell Protein and Mushrooms

oligonucleotides held to maltose binding

protein gene. This gene was expressed in E.
coli under the transcriptional control of tac
promoter. The production of this artificial
protein accounts to around 12% of the
intracellular proteins. However, the large
scale production of artificial protein by
rumen bacteria is yet to be clearly
established and commercialized.

Mushrooms:
Mushrooms are fungi belonging to the
classes basidiomycetes (Agaricus sp,
Auricularia sp, Tremella sp) and
ascomycetes (Morchella sp, Tuber sp).
Majority of edible mushrooms are the
species of basidomyces. It is estimated that
there are around 4,000 species of
basidiomyces. Of these, around 200 are
edible, and a dozen of them are cultivated on
large scale. Some of the most important
edible mushrooms, their common names
and the substrates used are given in Table
29.3.

We use cookies on our website to give you the most relevant experience by remembering your preferences
and repeat visits. By clicking “Accept”, you consent to the use of ALL the cookies.
Do not sell my personal information.

The cultivation
Cookie Settings Accept
of edible mushrooms is one
of the rare examples of a microbial culture
https://www.biologydiscussion.com/single-cell-protein/production-of-single-cell-protein-and-mushrooms/10392 26/43
1/28/24, 10:22 AM Production of Single-Cell Protein and Mushrooms

wherein the cultivated macroscopic

organism itself is directly used as human
food. Mushroom growing is one of the
fastest developing biotechnological
industries world over. Further growth of
mushroom industry is expected for the
production of enzymes, and pharmaceutical
compounds, including antitumor agents and
antibiotics.

Poisonous mushrooms:

There are certain poisonous mushrooms

also. They usually possess unpleasant taste
and odour. These mushrooms produce some
poisonous substances like phallin and
muscarine. The examples of poisonous
mushrooms are Amanita phalloides, A.
muscaria, A. viraosa, Lepiota morgani and
Boletus satanas.

Nutritive value of edible mushrooms:

Some people regard edible mushrooms as

vegetable meat. Mushrooms contain 80-
90% water, depending on the growth
conditions (temperature, humidity). Edible
mushrooms are rich sources of protein (35-
45% of dry weight). However, all these
We use cookies on our website to give you the most relevant experience by remembering your preferences
proteins are not easily digestible by humans.
and repeat visits. By clicking “Accept”, you consent to the use of ALL the cookies.
Mushrooms
Do not sell also contain
my personal information. fats and free fatty
acids (7-10%), carbohydrates (5-15%) and
Cookie Settings Accept
minerals in good concentration. Certain

https://www.biologydiscussion.com/single-cell-protein/production-of-single-cell-protein-and-mushrooms/10392 27/43
1/28/24, 10:22 AM Production of Single-Cell Protein and Mushrooms

undesirable substances may also be present

in edible mushrooms e.g. cadmium,
chromium.

Many delicious recipes of edible mushrooms

can be prepared. This actually depends on
the dietary habits of the people. Some of the
common recipes are mushroom soup,
mushroom paneer, mushroom pulao, and
mushroom omelets.

Advantages of edible mushroom

biotechnology:

1. Mushrooms can be produced by utilizing

cheap and often waste substrates (industrial
and wood wastes).

2. They are of high nutritive value being rich

in proteins, vitamins and minerals.

3. Many delicious recipes can be prepared

from mushrooms.

4. Due to low carbohydrate content,

consumption of mushrooms is advocated to
diabetic patients.

Production of Edible Mushrooms:

Mushroom
We use cookies production
on our website isthe
to give you basically a experience by remembering your preferences
most relevant
and repeat visits. By clicking “Accept”, you consent to the use of ALL the cookies.
fermentation process. This is mostly carried
Do not sell my personal information.
out by solid-substrate fermentation. A wide
Cookie Settings Accept
range of substrates (straw, saw dust,

https://www.biologydiscussion.com/single-cell-protein/production-of-single-cell-protein-and-mushrooms/10392 28/43
1/28/24, 10:22 AM Production of Single-Cell Protein and Mushrooms

compost, wooden logs) depending the

organism can be used (Refer Table 29.3).
Mushroom production is a good example of
a low technology utilization in an otherwise
sophisticated modern biotechnology.

The most common edible mushroom

cultivated world over (that may constitute
about 20% world mushroom produce) is the
white button mushroom, Agaricus bisporus.
Lentinula edodes is the second most
cultivated mushroom in the world. The
substrates straw, compost or horse manure
can be used. The substrate selection depends
on the local factors.

A schematic representation of mushroom

production is depicted in Fig. 29.5. The
compost with desired formulation is
prepared and sterilized. It is spread into the
trays which are then transferred to
production room and inoculated with
spawn. Spawn is the term used for the
mushroom
We use cookies inoculum
on our website to give containing spores
you the most relevant experience by remembering your preferences
and repeat
and/or small pieces of fruiting body. of ALL the cookies.
visits. By clicking “Accept”, you consent to the use
Do not sell my personal information.

Cookie Settings Accept

https://www.biologydiscussion.com/single-cell-protein/production-of-single-cell-protein-and-mushrooms/10392 29/43
1/28/24, 10:22 AM Production of Single-Cell Protein and Mushrooms

After inoculation (spawning), the culture is

maintained at optimal growth conditions.
The trays are regularly watered to maintain
70-80% humidity. The ideal temperature is
about 15°C, and pH about 7.0. It takes about
7-10 days for each crop of mushroom
production. It is possible to have 3-4 crops,
before terminating the production process.
The mushrooms can be harvested and
marketed.

Mushrooms have a very short life 8-12

hours, unless stored at low temperature
(refrigerator 2-5°C). Therefore, they should
be immediately consumed, stored or canned.
We use cookies on our website to give you the most relevant experience by remembering your preferences
Variations
and repeat in culturing
visits. By clicking mushrooms:
“Accept”, you The
consent to the use of ALL the cookies.
Do not sell my personal information.
production of mushrooms is highly variable
Cookieand mostlyAccept
Settings depends on the organism and
the substrate used, besides several other
https://www.biologydiscussion.com/single-cell-protein/production-of-single-cell-protein-and-mushrooms/10392 30/43
1/28/24, 10:22 AM Production of Single-Cell Protein and Mushrooms

local factors. There are distinct differences

in the mushroom cultivation methods
between different countries. For instance,
garden and field cultivation methods are
used in Europe, while in USA, cave and
house cultivation techniques are employed.

Some mushrooms (e.g. Volirariella sp) are

suitable for cultivation in summer and rainy
reason while others grow well in winter
(Agaricus bisporus, Pleurotus sp). It is
however, possible to grow these mushrooms
any time in a year with appropriate
temperature and humidity control
arrangements.

Related Articles:
1. Single Cell Protein (SCP): Production
and Advantages
2. Substrates used for SCP Production |
Food Microbiology

You May Like Sponsored Links by Taboola

High Sugar level Problem ? Read Here Before It

gets deleted
Sheopals Diabetes Care

Punjab: Insurance That Pays You for Being Fit

Aditya Birla Capital Get Offer

We use cookies on our website to give you the most relevant experience by remembering your preferences
and repeat visits. By clicking “Accept”, you consent to the use of ALL the cookies.
Reduce
Do not sell Bellyinformation.
my personal Fat Within Few Days
Tummy Trimmer Shop Now
Cookie Settings Accept

https://www.biologydiscussion.com/single-cell-protein/production-of-single-cell-protein-and-mushrooms/10392 31/43