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MICRO PRE TEST - Unlocked
MICRO PRE TEST - Unlocked
MICRO PRE TEST - Unlocked
INSTRUCTION: Select the correct answer for each of the Thiosulfate-Citrate Bile Salts Sucrose
following questions. Mark only one answer for each item by agar (TCBS) - selective for VIBRIO due to
shading the box corresponding to the letter of your choice on the presence of thiosulfate and bile salts.
the answer sheet provided. - maintain moist environment, with
STRICTLY NO ERASURES ALLOWED. nutrients to maintain growth.
- maintains organisms’ viability during
1. Which of the following generally refers to instances transport.
where there is a disproportionately larger number of JEMBEC/TRANSGROW - _________
infected individuals in a fairly short amount of time? STUARTS’S - Viral Transport Media
a. Endemic CARY BLAIR - __________________
b. Epidemic
c. Pandemic
5. Stain for capsule, except:
d. Outbreak
a. Neisser
b. Muir
A larger than normal number of diseased or c. Anthony’s
infected individuals that occurs over a short d. Hiss
period.
Muir, Anthony’s, Tyler, Hiss, Welch’s, Grins
An epidemic that spans the world.
Dorner’s, Schaeffer-fulton, Wirtz &
A larger than normal number of diseased or Conklin, Heat and Acetic acid method
infected individuals in a particular location.
Fisher and Conn, Gray’s, Loeffler’s,
A disease constantly present at some rate of Leifson, Caesares Gil, Van ermenger
occurrence in a particular location
Feulgen, Acridine Orange
Wayson
2. Which of the following toxins requires cell death for
Levaditi, Fontana, Warthin starry
release?
Gimenez, Macchiavelo, Giemsa
a. Endotoxin
b. Neurotoxin Neisser, Alberts, Ljubinsky, Lamb
c. Enterotoxin
d. Cytotoxin 6. Correct arrangement in PCR Amplification
a. Annealing, Denaturation, Extension
EXOTOXIN ENDOTOXIN b. Annealing, Denaturation, Exit
c. Denaturation, Annealing, Exit
d. Denaturation, Annealing, Extension
94 °C
50-58 °C
Composition: _________ Composition: _______________ 72 °C
15. Which of the following species of Bacillus is 22. Which of the following refers to a biochemical test
nonmotile? used to separate Staphylococci, Micrococci from
a. B. cereus
Streptococci?
b. B. subtilis
a. Coagulase
c. B. anthracis
b. Catalase
d. B. thuringiensis
c. Modified Oxidase
d. Bacitracin
16. Causative agent of erysipeloid
_______________- A biochemical test used to separate
a. S. pyogenes
Staphylococci (+), Micrococci (+) from Streptococci (-)
b. E. rhusiopathiae
➢ (+) Result : ____________________
c. C. minutissimum
➢ Uses ___________________________
d. Lactobacillus
➢ Do not use colonies from BAP (False Positive
Reaction); can use any media except _________
17. CCFA is used for the recovery of __________
a. Yersinia enterocolitica
b. Yersinia pestis
c. Clostridium perfringens 3% H2O2 for ___________________________
d. Clostridium difficile 30% H2O2 for ___________________________
15% H2O2 for _______________________
18. Minimal branching aerial hyphae, except:
a. Nocardia 23. Which of the following must be incubated in a
b. Rhodococcus microaerophilic environment for optimal recovery of
c. Gordonia the organism?
d. Tsukamurella a. E. coli
b. P. aeruginosa
c. C. jejuni
d. P. mirabilis
24. In the following pairs of organisms, which two are 29. Causative agent of Cat-scratch disease
hardest to distinguish from each other by Gram a. B. bacilliformis
stain? b. B. quintana
a. Micrococcus, Streptoccous c. B. hensenlae
b. Neisseria, Microccus d. B. elizabethae
c. Bacillus, Sarcina
d. Clostridium, Veilonella Species DISEASE
Bartonella ________________
Micrococcus, Staphylococcus, Streptococcus bacilliformis Orroya or Verruga peruana
Rochalimaea/Barto ________________, endocarditis
Peptococcus, Peptostreptoccocus, Sarcina, nella quintana
Ruminococcus, Coprococcus
Bartonella ________________________________
Branhamella, Neisseria
hensenlae Endocarditis
Veilonella, Acidaminococcus, Megasphera Peliosis Hepatitis
Bartonella ________________________________
Bacillus, Corynebacterium, Erysipelothrix, clarridgeiae NO endocarditis
Listeria, Mycobacterium, Nocardia Bartonella Endocarditis
Actinomyces, Clostridium, elizabethae
Propionobacterium, Bifidobacterium,
Eubacterium, Lactobacillus, Mobiluncus
30. Which of the following dyes are used to differentiate
Acinetobacter, Aeromonas, Alcaligenes,
Bordetella, Brucella Enterics, Francisella, Brucella species?
Legionella, Pasteurella, Pseudomonas, a. Wright and Giemsa
Vibrio b. Methylene blue and Basic Fuchsin
Fusobacterium, Bacteroides, Prevotella, c. Thionine and Basic Fuchsin
Porphyromonas d. Basic Fuchsin and Giemsa
25. Stage of bacterial growth where the organisms Brucella spp Host H2S Prod. Basic Thionine
become susceptible to antibiotics Fuchsin
a. Lag B. abortus +
b. Log B. melitensis +
c. Stationary B. suis -
d. Death B. canis +/-
Increase in size but not in number but
bacterial enzyme is very active 31. VP (+) and MR (-) Enterobacteriaceae
Growth rate is 0% a. Klebsiella
Stage where the organisms are most b. E. coli
metabolically active c. Salmonella
Growth rate starts to increase d. Shigella
____________________________________
____________________________________ 32. Also known as the Hansen’s bacillus
The number of dying organisms is equal to a. M. tuberculosis
the number of viable organisms. b. M. kansasii
NO net growth c. M. leprae
The number of dying organisms is greater d. M. terrae
than the number of viable organisms.
Mycobacterium tuberculosis Mycobacterium gordonae
26. Organism with yellow colonies on TCBS -________________ -________________
a. V. cholerae
b. V. parahaemolyticus Mycobacterium leprae Mycobacterium terrae
c. V. mimicus -________________ -________________
d. V. vulnificus
Mycobacterium kansasii M. intracellulare
Species TCBS -________________ -________________
Vibrio cholerae
Vibrio parahaemolyticus Mycobacterium phlei
Vibrio vulnificus -________________
Vibrio mimicus
Vibrio alginolyticus 33. Part of MTB Complex, except:
Vibrio fluvialis a. M. bovis
Vibrio furnissii b. M. africanum
c. M. kansasii
27. The species of Vibrio closely associated with rapidly d. M. microti
progressing wound infections seen in patients with
underlying liver disease is Mycobacterium tuberculosis complex – causes TB
a. V. alginolyticus 1. M. ________________– pulmonary TB
b. V. cholerae 2. M. ______– intestinal tuberculosis, BCG vaccine source
c. V. vulnificus 3. M. ________________– pulmonary TB in Africa
d. V. parahaemolyticus 4. M. ________________– smooth strain of M. tuberculosis
5. M. _______– isolated from TB patients in both
28. Which of the following Haemophilus does not immunocompetent and immunocompromised
require X and V Factor?
a. H. influenzae
b. H. ducreyi 34. Vector of B. burgdorferi
c. H. parahaemophilus a. Ticks
d. H. aphrophilus b. Mites
c. Louse
V-FACTOR X-FACTOR d. Chigger
H.influenzae
H.aegypticus Lyme Disease Stages
H.haemolyticus STAGE 1; ________________________________)
H.parainfluenzae STAGE 2: Most important features: Neurologic disorders
H.parahaemolyticus (Meningitis) and Carditis
H.paraphrophilus STAGE 3:________________________________________
H.aphrophilus
H.ducreyi
48. Lumpy jaw is caused by: 54. FECT arrange from top to bottom
a. C. tetani a. Ether > Debris > Formalin > Sediment
b. A. israelii b. Debris > Formalin > Ether> Sediment
c. B. anthracis c. Formalin > Debris > Ether > Sediment
d. C. botulinum d. Ether > Formalin > Debris > Sediment
2. ___________
-two species live together, but one specie benefits from
relationship without harming or benefiting one another.
74. All of the following fungi can cause -Large, round oval, yeast cell (15-30um)
Phaehypomycosis, except: with multiple buds attached to mother cell
a. Alternaria by narrow constrictions resembling
b. Bipolaris “Mariner’s Wheel” & “Mickey mouse
cup”
c. Wangiella
d. Fonsecaea
Fungal topography
furrowed or convoluted
slightly raised in the center),
75. All of the following can cause White Granules furrows radiate out from the
Mycetoma, except: center).
a. Madurella Texture of fungal colony
b. Scedosporidium loose, high aerial
c. Pseudoallescheria mycelium
d. Acremonium low aerial mycelium
resembling a
White Granules Mycetoma Black Granules Mycetoma velvet cloth
smooth surface with no
aerial
mycelium
dense, powdery, resembling
sugar
granules
76. Which dematiaceous mold forms flask-shaped high aerial mycelium that
phialides, each with a flask-shaped collarette? appears
a. Phialophora spp. slightly matted down).
b. Exophiala spp.
c. Wangiella spp. 82. What is the 2nd step in the identification of unknown
d. All of these options yeast isolate?
a. Germ tube
77. Trichophyton rubrum and T. mentagrophytes may be b. Morphology on cornmeal agar
differentiated by the c. CHO Utilization
a. Consistently different appearance of their colonies d. India Ink
b. Endothrix hair infection produced by T. rubrum
c. Fluorescence of hairs infected with T. rubrum Identification of unknown yeast isolate
d. In vitro hair penetration by T. mentagrophytes
First step
Second step
Species Urease Test Hair-baiting
Most common conventional
Trichophyton
definitive identification
mentagrophytes
Trichophyton rubrum 83. Infectious form of fungi
a. Yeast
78. Hyaline septate hyphae, branched or unbranched b. Mold
conidiophores, and multicelled banana-shaped c. Sexual
conidia are characteristic of which of the following? d. Asexual
a. Fusarium
b. Curvularia YEAST MOLD
c. Acremonium ✓ ______________ ✓ ______________,
d. Trichophyton ✓ Colonies are moist filamentous
creamy, opaque and ✓ Colonies are fluffy,
79. All of the following fungi can produce arthroconidia, pasty cottony, wooly or
except: ✓ Grows at 35-37 degC powdery
a. Coccidiodes ✓ Usually the ✓ Grows at ROOM
b. Trichosporon ___________________ TEMPERATURE
c. Geotrichum _________ ✓ ______________
d. Candida
84. A black pigment produced by colonies growing on
80. Which of the following refers to a large yeast with bird seed agar is due to
single bud on broad base? a. Urease
a. Paracoccidioides brasiliensis b. Phenol oxidase
b. Sporothrix schenckii c. Sucrose assimilation
c. Coccidioides immitis d. Arthroconidia production
d. B. dermatitidis
85. Used as a stain-preservative for fungi
Fungal Agent 35 degC (Yeast) a. PAS Stain.
-Thick-walled, large yeast with single bud b. Gomori Methenamine Silver
on BROAD BASE (8-12um) c. Lactophenol cotton blue
d. Giemsa stain
-Large, round thick walled SPHERULES
(30-60 um) with endospores; Cob-web -LACTIC ACID: PHENOL:
like colonies -COTTON BLUE:
-Small budding, oval yeast cells (2-5 um);
Lactophenol Cotton Blue - stain-preservative; ID of
INTRACELLULAR MACROPHAGES
seen with Giemsa or Wright’s MOLDS
*H. duboisii - Double cell/Figure *; causes
african histoplasmosis
86. Viral transport medium containing penicillin, 98. Most common symptoms of COVID-19, except:
gentamicin and amphotericin is used to collect and a. Fever
transport specimen for virus culture because this b. Dry cough
medium: c. Tiredness
a. Enables rapid viral growth during transport d. Headache
time
b. Inhibits bacterial and fungal growth Most common symptoms: ____________________________
c. Destroys nonpathogenic viruses Least common symptoms: Aches and pain, sore throat,
d. Inhibits complement-fixing antibodies diarrhea, conjunctivitis, ______________, loss of taste or smell,
a rash on skin, or discoloration of finger or toes.
87. EBV is associated with which of the following? Source: WHO
a. Chickenpox
b. Hodgkin lymphoma 99. Many of the symptoms of COVID-19 are common to
c. Burkitt lymphoma other respiratory or viral illnesses, ______ appears
d. Smallpox to be more specific to COVID-19
a. Sore throat
88. Colds and other acute respiratory diseases are most b. Fever
often associated with: c. Loss of ability to smell
a. EBV d. Shortness of breath
b. Adenovirus
c. Coxsackie virus 100. Few persons with SARS were identified as
d. Reovirus a. Super human
b. Super spreaders
89. The genus of virus associated with anogenital warts, c. Case
cervical dysplasia and neoplasia is: d. Contact
a. HSV .
b. HPV
c. CMV
d. RSV